Restriction map for
pF1KB6167
(Circular) (Six-base) MAPSORT of: pF1KB6167.seq Check: 9269 from: 1 to: 5060
pF1KB6167 5060 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 309 1120 1687 309
Size: 811 567 3682
Fragments arranged by size:
3682 811 567
AccI GT'mk_AC
Cuts at: 2098 2098
Size: 5060
Acc65I G'GTAC_C
Cuts at: 2076 2076
Size: 5060
AclI AA'CG_TT
Cuts at: 4900 4900
Size: 5060
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1019 1627 1984 2885 3317 4064 4332 376
Size: 643 608 357 901 432 747 268 1104
Fragments arranged by size:
1104 901 747 643 608 432 357 268
AflIII A'CryG_T
Cuts at: 2412 3516 4493 2412
Size: 1104 977 2979
Fragments arranged by size:
2979 1104 977
AgeI A'CCGG_T
Cuts at: 3448 3448
Size: 5060
AlwNI CAG_nnn'CTG
Cuts at: 1116 2365 3932 1116
Size: 1249 1567 2244
Fragments arranged by size:
2244 1567 1249
ApaLI G'TGCA_C
Cuts at: 3830 3830
Size: 5060
ApoI r'AATT_y
Cuts at: 173 179 572 908 1121 1199 1962 2064 4413
Size: 6 393 336 213 78 763 102 2349
Cuts at: 4413 4669 173
Size: 256 564
Fragments arranged by size:
2349 763 564 393 336 256 213 102
78 6
AseI AT'TA_AT
Cuts at: 20 550 1031 20
Size: 530 481 4049
Fragments arranged by size:
4049 530 481
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5060
AvaI C'yCGr_G
Cuts at: 2080 2080
Size: 5060
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1750 1783 1750
Size: 33 5027
BamHI G'GATC_C
Cuts at: 2085 2085
Size: 5060
BanI G'GyrC_C
Cuts at: 2076 2490 2738 2773 2076
Size: 414 248 35 4363
Fragments arranged by size:
4363 414 248 35
BanII G_rGCy'C
Cuts at: 2074 3104 2074
Size: 1030 4030
BbeI G_GCGC'C
Cuts at: 2494 2742 2494
Size: 248 4812
BbsI GAAGACnn'nnnn_
Cuts at: 548 1784 548
Size: 1236 3824
BciVI GTATCCnnnnn_n'
Cuts at: 2958 3719 5060 2958
Size: 761 1341 2958
Fragments arranged by size:
2958 1341 761
BclI T'GATC_A
Cuts at: 2580 2580
Size: 5060
BglII A'GATC_T
Cuts at: 1590 1728 2575 4612 1590
Size: 138 847 2037 2038
Fragments arranged by size:
2038 2037 847 138
BlpI GC'TnA_GC
Cuts at: 2172 2172
Size: 5060
Bme1580I G_kGCm'C
Cuts at: 2685 2778 3834 2685
Size: 93 1056 3911
Fragments arranged by size:
3911 1056 93
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5060
BmrI ACTGGGnnnn_n'
Cuts at: 2272 2353 2688 4832 2272
Size: 81 335 2144 2500
Fragments arranged by size:
2500 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 764 3427 764
Size: 2663 2397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 1753 662
Size: 1091 3969
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2236 3115 3607 3905 2236
Size: 879 492 298 3391
Fragments arranged by size:
3391 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4764 4764
Size: 5060
BsaAI yAC'GTr
Cuts at: 2413 2413
Size: 5060
BsaBI GATnn'nnATC
Cuts at: 6 2574 4611 6
Size: 2568 2037 455
Fragments arranged by size:
2568 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 2491 2739 3441 4937 112
Size: 2379 248 702 1496 235
Fragments arranged by size:
2379 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 2249 2770 3448 3722 3869 4280 2249
Size: 521 678 274 147 411 3029
Fragments arranged by size:
3029 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2485 3243 3820 4316 2485
Size: 758 577 496 3229
Fragments arranged by size:
3229 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1340 1697 1340
Size: 357 4703
BsiEI CG_ry'CG
Cuts at: 104 2602 2648 3856 104
Size: 2498 46 1208 1308
Fragments arranged by size:
2498 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2074 2852 3042 3834 2074
Size: 778 190 792 3300
Fragments arranged by size:
3300 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1919 1919
Size: 5060
Bsp1286I G_dGCh'C
Cuts at: 2074 2685 2778 2852 3042 3104 3834 2074
Size: 611 93 74 190 62 730 3300
Fragments arranged by size:
3300 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4280 4280
Size: 5060
BspHI T'CATG_A
Cuts at: 4236 4236
Size: 5060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 309 1120 1312 1687 2110 2324 2626 3007 309
Size: 811 192 375 423 214 302 381 2362
Fragments arranged by size:
2362 811 423 381 375 302 214 192
BsrBI CCG'CTC
Cuts at: 50 3352 3406 4890 5055 50
Size: 3302 54 1484 165 55
Fragments arranged by size:
3302 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 288 2972 288
Size: 2684 2376
BsrFI r'CCGG_y
Cuts at: 3058 3239 3448 3058
Size: 181 209 4670
Fragments arranged by size:
4670 209 181
BssSI C'ACGA_G
Cuts at: 1721 3331 3689 1721
Size: 1610 358 3092
Fragments arranged by size:
3092 1610 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1222 2437 1222
Size: 1215 3845
BstBI TT'CG_AA
Cuts at: 3421 3421
Size: 5060
BstXI CCAn_nnnn'nTGG
Cuts at: 1312 1312
Size: 5060
BstYI r'GATC_y
Cuts at: 1 1590 1601 1728 2085 2552 2575 2910 3156
Size: 1589 11 127 357 467 23 335 246
Cuts at: 3156 4168 4254 4266 4612 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1589 1012 467 449 357 346 335 246
127 86 23 12 11
BtgI C'CryG_G
Cuts at: 107 328 1289 107
Size: 221 961 3878
Fragments arranged by size:
3878 961 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3010 3191 3354 3010
Size: 181 163 4716
Fragments arranged by size:
4716 181 163
BtsI GCAGTG_nn'
Cuts at: 256 771 2303 256
Size: 515 1532 3013
Fragments arranged by size:
3013 1532 515
ClaI AT'CG_AT
Cuts at: 2045 2045
Size: 5060
DraI TTT'AAA
Cuts at: 369 560 2059 369
Size: 191 1499 3370
Fragments arranged by size:
3370 1499 191
DrdI GACnn_nn'nnGTC
Cuts at: 1322 2766 3624 1322
Size: 1444 858 2758
Fragments arranged by size:
2758 1444 858
EaeI y'GGCC_r
Cuts at: 2645 2819 3210 3237 4582 2645
Size: 174 391 27 1345 3123
Fragments arranged by size:
3123 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2645 2645
Size: 5060
EarI CTCTTCn'nnn_
Cuts at: 3083 3293 3083
Size: 210 4850
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3268 3578 3724 4870 3268
Size: 310 146 1146 3458
Fragments arranged by size:
3458 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2072 2072
Size: 5060
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 662 1019 1627 1753 1984 2885 3317 4064
Size: 286 357 608 126 231 901 432 747
Cuts at: 4064 4332 376
Size: 268 1104
Fragments arranged by size:
1104 901 747 608 432 357 286 268
231 126
EcoNI CCTnn'n_nnAGG
Cuts at: 452 1609 452
Size: 1157 3903
EcoO109I rG'GnC_Cy
Cuts at: 1825 2199 1825
Size: 374 4686
EcoRI G'AATT_C
Cuts at: 1121 2064 1121
Size: 943 4117
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1855 3079 1855
Size: 1224 3836
FspI TGC'GCA
Cuts at: 2841 2841
Size: 5060
HaeII r_GCGC'y
Cuts at: 2494 2742 3764 4743 2494
Size: 248 1022 979 2811
Fragments arranged by size:
2811 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 885 917 1423 1455 1580 1612 2902 2932 2934
Size: 32 506 32 125 32 1290 30 2
Cuts at: 2934 2964 885
Size: 30 2981
Fragments arranged by size:
2981 1290 506 125 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2099 2099
Size: 5060
HindIII A'AGCT_T
Cuts at: 1423 1423
Size: 5060
KasI G'GCGC_C
Cuts at: 2490 2738 2490
Size: 248 4812
KpnI G_GTAC'C
Cuts at: 2080 2080
Size: 5060
MluI A'CGCG_T
Cuts at: 4493 4493
Size: 5060
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3731 3915 3731
Size: 184 4876
MscI TGG'CCA
Cuts at: 2821 2821
Size: 5060
MslI CAynn'nnrTG
Cuts at: 1130 1493 3049 3176 1130
Size: 363 1556 127 3014
Fragments arranged by size:
3014 1556 363 127
MspA1I CmG'CkG
Cuts at: 2171 2485 2845 3858 4103 2171
Size: 314 360 1013 245 3128
Fragments arranged by size:
3128 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3241 3241
Size: 5060
NarI GG'CG_CC
Cuts at: 2491 2739 2491
Size: 248 4812
NcoI C'CATG_G
Cuts at: 107 328 1289 107
Size: 221 961 3878
Fragments arranged by size:
3878 961 221
NgoMIV G'CCGG_C
Cuts at: 3239 3239
Size: 5060
NspI r_CATG'y
Cuts at: 166 616 1520 1861 1998 2113 2613 3520 166
Size: 450 904 341 137 115 500 907 1706
Fragments arranged by size:
1706 907 904 500 450 341 137 115
PciI A'CATG_T
Cuts at: 3516 3516
Size: 5060
PflMI CCAn_nnn'nTGG
Cuts at: 334 552 334
Size: 218 4842
PmeI GTTT'AAAC
Cuts at: 2059 2059
Size: 5060
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 4199
Size: 32 5028
PpuMI rG'GwC_Cy
Cuts at: 1825 1825
Size: 5060
PsiI TTA'TAA
Cuts at: 812 812
Size: 5060
PstI C_TGCA'G
Cuts at: 1588 2107 2792 1588
Size: 519 685 3856
Fragments arranged by size:
3856 685 519
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5060
PvuII CAG'CTG
Cuts at: 2485 2845 2485
Size: 360 4700
RsrII CG'GwC_CG
Cuts at: 3255 3255
Size: 5060
SacI G_AGCT'C
Cuts at: 2074 2074
Size: 5060
SalI G'TCGA_C
Cuts at: 2097 2097
Size: 5060
SapI GCTCTTCn'nnn_
Cuts at: 3083 3293 3083
Size: 210 4850
SbfI CC_TGCA'GG
Cuts at: 2107 2107
Size: 5060
ScaI AGT'ACT
Cuts at: 1582 4577 1582
Size: 2995 2065
SfcI C'TryA_G
Cuts at: 33 449 777 1584 1782 2103 2788 3781 3972
Size: 416 328 807 198 321 685 993 191
Cuts at: 3972 33
Size: 1121
Fragments arranged by size:
1121 993 807 685 416 328 321 198
191
SfoI GGC'GCC
Cuts at: 2492 2740 2492
Size: 248 4812
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5060
SmaI CCC'GGG
Cuts at: 2082 2082
Size: 5060
SmlI C'TyrA_G
Cuts at: 2215 3130 3622 3884 2215
Size: 915 492 262 3391
Fragments arranged by size:
3391 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5060
SphI G_CATG'C
Cuts at: 2113 2613 2113
Size: 500 4560
StyI C'CwwG_G
Cuts at: 107 328 1289 2194 2548 107
Size: 221 961 905 354 2619
Fragments arranged by size:
2619 961 905 354 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2643 2776 3235 3444 2643
Size: 133 459 209 4259
Fragments arranged by size:
4259 459 209 133
TatI w'GTAC_w
Cuts at: 1355 1580 4575 1355
Size: 225 2995 1840
Fragments arranged by size:
2995 1840 225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4577 4609 4577
Size: 32 5028
Tth111I GACn'n_nGTC
Cuts at: 1881 2857 1881
Size: 976 4084
XbaI T'CTAG_A
Cuts at: 2091 2091
Size: 5060
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2555 2555
Size: 5060
XmaI C'CCGG_G
Cuts at: 2080 2080
Size: 5060
XmnI GAAnn'nnTTC
Cuts at: 323 1386 1897 323
Size: 1063 511 3486
Fragments arranged by size:
3486 1063 511
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsaXI BseRI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI