Restriction map for
pF1KB6162
(Circular) (Six-base) MAPSORT of: pF1KB6162.seq Check: 2906 from: 1 to: 5054
pF1KB6162 5054 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 1201 2092 1201
Size: 891 4163
Acc65I G'GTAC_C
Cuts at: 1429 2005 2070 1429
Size: 576 65 4413
Fragments arranged by size:
4413 576 65
AclI AA'CG_TT
Cuts at: 4894 4894
Size: 5054
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1587 2879 3311 4058 4326 1587
Size: 1292 432 747 268 2315
Fragments arranged by size:
2315 1292 747 432 268
AflIII A'CryG_T
Cuts at: 374 2406 3510 4487 374
Size: 2032 1104 977 941
Fragments arranged by size:
2032 1104 977 941
AgeI A'CCGG_T
Cuts at: 3442 3442
Size: 5054
AleI CACnn'nnGTG
Cuts at: 1657 1657
Size: 5054
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 625 625
Size: 5054
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 212 244 212
Size: 32 5022
AlwNI CAG_nnn'CTG
Cuts at: 1782 2359 3926 1782
Size: 577 1567 2910
Fragments arranged by size:
2910 1567 577
ApaLI G'TGCA_C
Cuts at: 3824 3824
Size: 5054
ApoI r'AATT_y
Cuts at: 322 2058 4407 4663 322
Size: 1736 2349 256 713
Fragments arranged by size:
2349 1736 713 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5054
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5054
AvaI C'yCGr_G
Cuts at: 143 215 481 2023 2074 143
Size: 72 266 1542 51 3123
Fragments arranged by size:
3123 1542 266 72 51
BamHI G'GATC_C
Cuts at: 1409 2079 1409
Size: 670 4384
BanI G'GyrC_C
Cuts at: 195 948 973 1242 1429 2005 2070 2484 2732
Size: 753 25 269 187 576 65 414 248
Cuts at: 2732 2767 195
Size: 35 2482
Fragments arranged by size:
2482 753 576 414 269 248 187 65
35 25
BanII G_rGCy'C
Cuts at: 1598 2068 3098 1598
Size: 470 1030 3554
Fragments arranged by size:
3554 1030 470
BbeI G_GCGC'C
Cuts at: 977 2488 2736 977
Size: 1511 248 3295
Fragments arranged by size:
3295 1511 248
BbsI GAAGACnn'nnnn_
Cuts at: 1573 1655 1688 1573
Size: 82 33 4939
Fragments arranged by size:
4939 82 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1251 1285 1251
Size: 34 5020
BciVI GTATCCnnnnn_n'
Cuts at: 2952 3713 5054 2952
Size: 761 1341 2952
Fragments arranged by size:
2952 1341 761
BclI T'GATC_A
Cuts at: 2574 2574
Size: 5054
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5054
BglII A'GATC_T
Cuts at: 2036 2569 4606 2036
Size: 533 2037 2484
Fragments arranged by size:
2484 2037 533
BlpI GC'TnA_GC
Cuts at: 2166 2166
Size: 5054
Bme1580I G_kGCm'C
Cuts at: 953 1159 1247 2679 2772 3828 953
Size: 206 88 1432 93 1056 2179
Fragments arranged by size:
2179 1432 1056 206 93 88
BmgBI CAC'GTC
Cuts at: 912 912
Size: 5054
BmrI ACTGGGnnnn_n'
Cuts at: 2266 2347 2682 4826 2266
Size: 81 335 2144 2494
Fragments arranged by size:
2494 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3421 3421
Size: 5054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1008 1815 1980 1008
Size: 807 165 4082
Fragments arranged by size:
4082 807 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 744 1048 1062 1492 2230 3109 3601 3899 744
Size: 304 14 430 738 879 492 298 1899
Fragments arranged by size:
1899 879 738 492 430 304 298 14
BsaI GGTCTCn'nnnn_
Cuts at: 718 4758 718
Size: 4040 1014
BsaAI yAC'GTr
Cuts at: 128 2407 128
Size: 2279 2775
BsaBI GATnn'nnATC
Cuts at: 6 2568 4605 6
Size: 2562 2037 455
Fragments arranged by size:
2562 2037 455
BsaHI Gr'CG_yC
Cuts at: 974 1295 2485 2733 3435 4931 974
Size: 321 1190 248 702 1496 1097
Fragments arranged by size:
1496 1190 1097 702 321 248
BsaWI w'CCGG_w
Cuts at: 2243 2764 3442 3716 3863 4274 2243
Size: 521 678 274 147 411 3023
Fragments arranged by size:
3023 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 212 242 1226 1256 212
Size: 30 984 30 4010
Fragments arranged by size:
4010 984 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 233 574 1084 233
Size: 341 510 4203
Fragments arranged by size:
4203 510 341
BseYI C'CCAG_C
Cuts at: 176 303 585 868 1083 1246 1667 2479 3237
Size: 127 282 283 215 163 421 812 758
Cuts at: 3237 3814 4310 176
Size: 577 496 920
Fragments arranged by size:
920 812 758 577 496 421 283 282
215 163 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 1537 731
Size: 806 4248
BsiEI CG_ry'CG
Cuts at: 104 363 2596 2642 3850 104
Size: 259 2233 46 1208 1308
Fragments arranged by size:
2233 1308 1208 259 46
BsiHKAI G_wGCw'C
Cuts at: 655 1386 1598 2068 2846 3036 3828 655
Size: 731 212 470 778 190 792 1881
Fragments arranged by size:
1881 792 778 731 470 212 190
BsmI GAATG_Cn'
Cuts at: 540 540
Size: 5054
BsmBI CGTCTCn'nnnn_
Cuts at: 1629 1629
Size: 5054
Bsp1286I G_dGCh'C
Cuts at: 655 953 1159 1247 1386 1598 2068 2679 2772
Size: 298 206 88 139 212 470 611 93
Cuts at: 2772 2846 3036 3098 3828 655
Size: 74 190 62 730 1881
Fragments arranged by size:
1881 730 611 470 298 212 206 190
139 93 88 74 62
BspEI T'CCGG_A
Cuts at: 4274 4274
Size: 5054
BspHI T'CATG_A
Cuts at: 896 4230 896
Size: 3334 1720
BspMI ACCTGCnnnn'nnnn_
Cuts at: 743 2104 2318 2620 3001 743
Size: 1361 214 302 381 2796
Fragments arranged by size:
2796 1361 381 302 214
BsrBI CCG'CTC
Cuts at: 50 248 1829 3346 3400 4884 5049 50
Size: 198 1581 1517 54 1484 165 55
Fragments arranged by size:
1581 1517 1484 198 165 55 54
BsrDI GCAATG_nn'
Cuts at: 417 1253 1304 2966 417
Size: 836 51 1662 2505
Fragments arranged by size:
2505 1662 836 51
BsrFI r'CCGG_y
Cuts at: 682 1975 3052 3233 3442 682
Size: 1293 1077 181 209 2294
Fragments arranged by size:
2294 1293 1077 209 181
BssSI C'ACGA_G
Cuts at: 648 3325 3683 648
Size: 2677 358 2019
Fragments arranged by size:
2677 2019 358
BstAPI GCAn_nnn'nTGC
Cuts at: 186 1395 2431 186
Size: 1209 1036 2809
Fragments arranged by size:
2809 1209 1036
BstBI TT'CG_AA
Cuts at: 3415 3415
Size: 5054
BstXI CCAn_nnnn'nTGG
Cuts at: 720 720
Size: 5054
BstYI r'GATC_y
Cuts at: 1 1409 2036 2079 2546 2569 2904 3150 4162
Size: 1408 627 43 467 23 335 246 1012
Cuts at: 4162 4248 4260 4606 1
Size: 86 12 346 449
Fragments arranged by size:
1408 1012 627 467 449 346 335 246
86 43 23 12
BstZ17I GTA'TAC
Cuts at: 1202 1202
Size: 5054
Bsu36I CC'TnA_GG
Cuts at: 1987 1987
Size: 5054
BtgI C'CryG_G
Cuts at: 107 424 738 1109 1764 107
Size: 317 314 371 655 3397
Fragments arranged by size:
3397 655 371 317 314
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 678 693 3004 3185 3348 678
Size: 15 2311 181 163 2384
Fragments arranged by size:
2384 2311 181 163 15
BtsI GCAGTG_nn'
Cuts at: 161 1491 1748 2297 161
Size: 1330 257 549 2918
Fragments arranged by size:
2918 1330 549 257
DraI TTT'AAA
Cuts at: 2053 2053
Size: 5054
DraIII CAC_nnn'GTG
Cuts at: 1291 1291
Size: 5054
DrdI GACnn_nn'nnGTC
Cuts at: 2760 3618 2760
Size: 858 4196
EaeI y'GGCC_r
Cuts at: 122 917 1112 1635 2639 2813 3204 3231 4576
Size: 795 195 523 1004 174 391 27 1345
Cuts at: 4576 122
Size: 600
Fragments arranged by size:
1345 1004 795 600 523 391 195 174
27
EagI C'GGCC_G
Cuts at: 2639 2639
Size: 5054
EarI CTCTTCn'nnn_
Cuts at: 449 503 2023 3077 3287 449
Size: 54 1520 1054 210 2216
Fragments arranged by size:
2216 1520 1054 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 370 1723 3262 3572 3718 4864 370
Size: 1353 1539 310 146 1146 560
Fragments arranged by size:
1539 1353 1146 560 310 146
EcoICRI GAG'CTC
Cuts at: 1596 2066 1596
Size: 470 4584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1008 1587 1815 1980 2879 3311 4058 4326 1008
Size: 579 228 165 899 432 747 268 1736
Fragments arranged by size:
1736 899 747 579 432 268 228 165
EcoNI CCTnn'n_nnAGG
Cuts at: 550 550
Size: 5054
EcoO109I rG'GnC_Cy
Cuts at: 755 1467 1678 2193 755
Size: 712 211 515 3616
Fragments arranged by size:
3616 712 515 211
EcoRI G'AATT_C
Cuts at: 322 2058 322
Size: 1736 3318
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 342 1188 1416 3073 342
Size: 846 228 1657 2323
Fragments arranged by size:
2323 1657 846 228
FspI TGC'GCA
Cuts at: 1804 2835 1804
Size: 1031 4023
HaeII r_GCGC'y
Cuts at: 258 546 977 1380 1725 2488 2736 3758 4737
Size: 288 431 403 345 763 248 1022 979
Cuts at: 4737 258
Size: 575
Fragments arranged by size:
1022 979 763 575 431 403 345 288
248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 325 357 952 984 2896 2926 2928 2958 325
Size: 32 595 32 1912 30 2 30 2421
Fragments arranged by size:
2421 1912 595 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2093 2093
Size: 5054
KasI G'GCGC_C
Cuts at: 973 2484 2732 973
Size: 1511 248 3295
Fragments arranged by size:
3295 1511 248
KpnI G_GTAC'C
Cuts at: 1433 2009 2074 1433
Size: 576 65 4413
Fragments arranged by size:
4413 576 65
MluI A'CGCG_T
Cuts at: 4487 4487
Size: 5054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1358 3725 3909 1358
Size: 2367 184 2503
Fragments arranged by size:
2503 2367 184
MscI TGG'CCA
Cuts at: 124 919 1114 2815 124
Size: 795 195 1701 2363
Fragments arranged by size:
2363 1701 795 195
MslI CAynn'nnrTG
Cuts at: 697 910 1135 1657 3043 3170 697
Size: 213 225 522 1386 127 2581
Fragments arranged by size:
2581 1386 522 225 213 127
MspA1I CmG'CkG
Cuts at: 618 740 1365 1481 2165 2479 2839 3852 4097
Size: 122 625 116 684 314 360 1013 245
Cuts at: 4097 618
Size: 1575
Fragments arranged by size:
1575 1013 684 625 360 314 245 122
116
NaeI GCC'GGC
Cuts at: 1977 3235 1977
Size: 1258 3796
NarI GG'CG_CC
Cuts at: 974 2485 2733 974
Size: 1511 248 3295
Fragments arranged by size:
3295 1511 248
NcoI C'CATG_G
Cuts at: 107 1109 107
Size: 1002 4052
NgoMIV G'CCGG_C
Cuts at: 1975 3233 1975
Size: 1258 3796
NruI TCG'CGA
Cuts at: 365 365
Size: 5054
NspI r_CATG'y
Cuts at: 378 1776 1791 2107 2607 3514 378
Size: 1398 15 316 500 907 1918
Fragments arranged by size:
1918 1398 907 500 316 15
PasI CC'CwG_GG
Cuts at: 1614 1614
Size: 5054
PciI A'CATG_T
Cuts at: 374 3510 374
Size: 3136 1918
PflMI CCAn_nnn'nTGG
Cuts at: 1674 1674
Size: 5054
PmeI GTTT'AAAC
Cuts at: 2053 2053
Size: 5054
PmlI CAC'GTG
Cuts at: 128 128
Size: 5054
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 212 244 4193 4225 212
Size: 32 3949 32 1041
Fragments arranged by size:
3949 1041 32 32
PpuMI rG'GwC_Cy
Cuts at: 755 1678 755
Size: 923 4131
PstI C_TGCA'G
Cuts at: 1451 2101 2786 1451
Size: 650 685 3719
Fragments arranged by size:
3719 685 650
PvuI CG_AT'CG
Cuts at: 104 363 104
Size: 259 4795
PvuII CAG'CTG
Cuts at: 2479 2839 2479
Size: 360 4694
RsrII CG'GwC_CG
Cuts at: 3249 3249
Size: 5054
SacI G_AGCT'C
Cuts at: 1598 2068 1598
Size: 470 4584
SacII CC_GC'GG
Cuts at: 741 741
Size: 5054
SalI G'TCGA_C
Cuts at: 2091 2091
Size: 5054
SanDI GG'GwC_CC
Cuts at: 1678 1678
Size: 5054
SapI GCTCTTCn'nnn_
Cuts at: 449 3077 3287 449
Size: 2628 210 2216
Fragments arranged by size:
2628 2216 210
SbfI CC_TGCA'GG
Cuts at: 2101 2101
Size: 5054
ScaI AGT'ACT
Cuts at: 352 4571 352
Size: 4219 835
SfcI C'TryA_G
Cuts at: 33 1447 2097 2782 3775 3966 33
Size: 1414 650 685 993 191 1121
Fragments arranged by size:
1414 1121 993 685 650 191
SfoI GGC'GCC
Cuts at: 975 2486 2734 975
Size: 1511 248 3295
Fragments arranged by size:
3295 1511 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5054
SmaI CCC'GGG
Cuts at: 2076 2076
Size: 5054
SmlI C'TyrA_G
Cuts at: 215 759 1041 1063 1471 2209 3124 3616 3878
Size: 544 282 22 408 738 915 492 262
Cuts at: 3878 215
Size: 1391
Fragments arranged by size:
1391 915 738 544 492 408 282 262
22
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5054
SphI G_CATG'C
Cuts at: 1776 2107 2607 1776
Size: 331 500 4223
Fragments arranged by size:
4223 500 331
StuI AGG'CCT
Cuts at: 119 445 796 119
Size: 326 351 4377
Fragments arranged by size:
4377 351 326
StyI C'CwwG_G
Cuts at: 107 1109 2188 2542 107
Size: 1002 1079 354 2619
Fragments arranged by size:
2619 1079 1002 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 536 693 2637 2770 3229 3438 536
Size: 157 1944 133 459 209 2152
Fragments arranged by size:
2152 1944 459 209 157 133
TatI w'GTAC_w
Cuts at: 350 822 1451 4569 350
Size: 472 629 3118 835
Fragments arranged by size:
3118 835 629 472
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1226 1258 4571 4603 1226
Size: 32 3313 32 1677
Fragments arranged by size:
3313 1677 32 32
Tth111I GACn'n_nGTC
Cuts at: 964 2851 964
Size: 1887 3167
XbaI T'CTAG_A
Cuts at: 1560 2085 1560
Size: 525 4529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2549 2549
Size: 5054
XhoI C'TCGA_G
Cuts at: 215 215
Size: 5054
XmaI C'CCGG_G
Cuts at: 2074 2074
Size: 5054
XmnI GAAnn'nnTTC
Cuts at: 322 322
Size: 5054
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BbvCI BfrBI BmtI Bpu10I BsiWI BsrGI BssHII
BstEII ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PfoI PshAI
PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI ZraI