Restriction map for
pF1KB6153
(Circular) (Six-base) MAPSORT of: pF1KB6153.seq Check: 8007 from: 1 to: 4904
pF1KB6153 4904 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1329 1329
Size: 4904
AccI GT'mk_AC
Cuts at: 1791 1942 1791
Size: 151 4753
Acc65I G'GTAC_C
Cuts at: 1920 1920
Size: 4904
AclI AA'CG_TT
Cuts at: 4744 4744
Size: 4904
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 563 767 880 1219 1351 1555 2729 3161 3908
Size: 204 113 339 132 204 1174 432 747
Cuts at: 3908 4176 563
Size: 268 1291
Fragments arranged by size:
1291 1174 747 432 339 268 204 204
132 113
AflII C'TTAA_G
Cuts at: 757 757
Size: 4904
AflIII A'CryG_T
Cuts at: 824 2256 3360 4337 824
Size: 1432 1104 977 1391
Fragments arranged by size:
1432 1391 1104 977
AgeI A'CCGG_T
Cuts at: 3292 3292
Size: 4904
AlwNI CAG_nnn'CTG
Cuts at: 1288 1344 1741 2209 3776 1288
Size: 56 397 468 1567 2416
Fragments arranged by size:
2416 1567 468 397 56
ApaLI G'TGCA_C
Cuts at: 1396 3674 1396
Size: 2278 2626
ApoI r'AATT_y
Cuts at: 363 703 789 817 1908 4257 4513 363
Size: 340 86 28 1091 2349 256 754
Fragments arranged by size:
2349 1091 754 340 256 86 28
AseI AT'TA_AT
Cuts at: 20 293 20
Size: 273 4631
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4904
AvaI C'yCGr_G
Cuts at: 1924 1924
Size: 4904
BamHI G'GATC_C
Cuts at: 1729 1929 1729
Size: 200 4704
BanI G'GyrC_C
Cuts at: 1456 1516 1920 2334 2582 2617 1456
Size: 60 404 414 248 35 3743
Fragments arranged by size:
3743 414 404 248 60 35
BanII G_rGCy'C
Cuts at: 1363 1918 2948 1363
Size: 555 1030 3319
Fragments arranged by size:
3319 1030 555
BbeI G_GCGC'C
Cuts at: 2338 2586 2338
Size: 248 4656
BbsI GAAGACnn'nnnn_
Cuts at: 269 569 1066 1478 269
Size: 300 497 412 3695
Fragments arranged by size:
3695 497 412 300
BbvCI CC'TCA_GC
Cuts at: 405 405
Size: 4904
BciVI GTATCCnnnnn_n'
Cuts at: 2802 3563 4904 2802
Size: 761 1341 2802
Fragments arranged by size:
2802 1341 761
BclI T'GATC_A
Cuts at: 2424 2424
Size: 4904
BfrBI ATG'CAT
Cuts at: 672 1881 672
Size: 1209 3695
BglI GCCn_nnn'nGGC
Cuts at: 508 508
Size: 4904
BglII A'GATC_T
Cuts at: 867 1811 2419 4456 867
Size: 944 608 2037 1315
Fragments arranged by size:
2037 1315 944 608
BlpI GC'TnA_GC
Cuts at: 2016 2016
Size: 4904
Bme1580I G_kGCm'C
Cuts at: 634 1400 2529 2622 3678 634
Size: 766 1129 93 1056 1860
Fragments arranged by size:
1860 1129 1056 766 93
BmrI ACTGGGnnnn_n'
Cuts at: 2116 2197 2532 4676 2116
Size: 81 335 2144 2344
Fragments arranged by size:
2344 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1286 3271 1286
Size: 1985 2919
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 184
Size: 4904
Bpu10I CC'TnA_GC
Cuts at: 405 405
Size: 4904
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2080 2959 3451 3749 2080
Size: 879 492 298 3235
Fragments arranged by size:
3235 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4608 4608
Size: 4904
BsaAI yAC'GTr
Cuts at: 825 2257 825
Size: 1432 3472
BsaBI GATnn'nnATC
Cuts at: 6 1742 2418 4455 6
Size: 1736 676 2037 455
Fragments arranged by size:
2037 1736 676 455
BsaHI Gr'CG_yC
Cuts at: 2335 2583 3285 4781 2335
Size: 248 702 1496 2458
Fragments arranged by size:
2458 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2093 2614 3292 3566 3713 4124 2093
Size: 521 678 274 147 411 2873
Fragments arranged by size:
2873 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1015 1045 1440 1470 1015
Size: 30 395 30 4449
Fragments arranged by size:
4449 395 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1039 1039
Size: 4904
BseYI C'CCAG_C
Cuts at: 2329 3087 3664 4160 2329
Size: 758 577 496 3073
Fragments arranged by size:
3073 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 833 833
Size: 4904
BsiEI CG_ry'CG
Cuts at: 104 1017 2446 2492 3700 104
Size: 913 1429 46 1208 1308
Fragments arranged by size:
1429 1308 1208 913 46
BsiHKAI G_wGCw'C
Cuts at: 1400 1918 2696 2886 3678 1400
Size: 518 778 190 792 2626
Fragments arranged by size:
2626 792 778 518 190
BsmI GAATG_Cn'
Cuts at: 1219 1219
Size: 4904
Bsp1286I G_dGCh'C
Cuts at: 634 1363 1400 1918 2529 2622 2696 2886 2948
Size: 729 37 518 611 93 74 190 62
Cuts at: 2948 3678 634
Size: 730 1860
Fragments arranged by size:
1860 730 729 611 518 190 93 74
62 37
BspEI T'CCGG_A
Cuts at: 4124 4124
Size: 4904
BspHI T'CATG_A
Cuts at: 4080 4080
Size: 4904
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1329 1954 2168 2470 2851 1329
Size: 625 214 302 381 3382
Fragments arranged by size:
3382 625 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3196 3250 4734 4899 50
Size: 3146 54 1484 165 55
Fragments arranged by size:
3146 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2816 2816
Size: 4904
BsrFI r'CCGG_y
Cuts at: 2902 3083 3292 2902
Size: 181 209 4514
Fragments arranged by size:
4514 209 181
BsrGI T'GTAC_A
Cuts at: 372 372
Size: 4904
BssSI C'ACGA_G
Cuts at: 3175 3533 3175
Size: 358 4546
BstAPI GCAn_nnn'nTGC
Cuts at: 2281 2281
Size: 4904
BstBI TT'CG_AA
Cuts at: 3265 3265
Size: 4904
BstXI CCAn_nnnn'nTGG
Cuts at: 536 1005 1786 536
Size: 469 781 3654
Fragments arranged by size:
3654 781 469
BstYI r'GATC_y
Cuts at: 1 867 1729 1811 1929 2396 2419 2754 3000
Size: 866 862 82 118 467 23 335 246
Cuts at: 3000 4012 4098 4110 4456 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 866 862 467 449 346 335 246
118 86 82 23 12
Bsu36I CC'TnA_GG
Cuts at: 1261 1261
Size: 4904
BtgI C'CryG_G
Cuts at: 107 107
Size: 4904
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1840 2854 3035 3198 1840
Size: 1014 181 163 3546
Fragments arranged by size:
3546 1014 181 163
BtsI GCAGTG_nn'
Cuts at: 610 829 1010 1061 2147 610
Size: 219 181 51 1086 3367
Fragments arranged by size:
3367 1086 219 181 51
ClaI AT'CG_AT
Cuts at: 570 570
Size: 4904
DraI TTT'AAA
Cuts at: 1599 1903 1599
Size: 304 4600
DrdI GACnn_nn'nnGTC
Cuts at: 182 2610 3468 182
Size: 2428 858 1618
Fragments arranged by size:
2428 1618 858
EaeI y'GGCC_r
Cuts at: 1014 1890 2489 2663 3054 3081 4426 1014
Size: 876 599 174 391 27 1345 1492
Fragments arranged by size:
1492 1345 876 599 391 174 27
EagI C'GGCC_G
Cuts at: 1014 2489 1014
Size: 1475 3429
EarI CTCTTCn'nnn_
Cuts at: 968 1043 1147 2927 3137 968
Size: 75 104 1780 210 2735
Fragments arranged by size:
2735 1780 210 104 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3112 3422 3568 4714 3112
Size: 310 146 1146 3302
Fragments arranged by size:
3302 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1916 1916
Size: 4904
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 563 767 880 1219 1351 1555 2729 3161
Size: 379 204 113 339 132 204 1174 432
Cuts at: 3161 3908 4176 184
Size: 747 268 912
Fragments arranged by size:
1174 912 747 432 379 339 268 204
204 132 113
EcoNI CCTnn'n_nnAGG
Cuts at: 256 256
Size: 4904
EcoO109I rG'GnC_Cy
Cuts at: 2043 2043
Size: 4904
EcoRI G'AATT_C
Cuts at: 363 1908 363
Size: 1545 3359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 282 772 2923 282
Size: 490 2151 2263
Fragments arranged by size:
2263 2151 490
FspI TGC'GCA
Cuts at: 2685 2685
Size: 4904
HaeII r_GCGC'y
Cuts at: 2338 2586 3608 4587 2338
Size: 248 1022 979 2655
Fragments arranged by size:
2655 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 559 591 946 978 1657 1689 1714 1746 2746
Size: 32 355 32 679 32 25 32 1000
Cuts at: 2746 2776 2778 2808 559
Size: 30 2 30 2655
Fragments arranged by size:
2655 1000 679 355 32 32 32 32
30 30 25 2
HincII GTy'rAC
Cuts at: 1632 1943 1632
Size: 311 4593
HindIII A'AGCT_T
Cuts at: 1201 1201
Size: 4904
KasI G'GCGC_C
Cuts at: 2334 2582 2334
Size: 248 4656
KpnI G_GTAC'C
Cuts at: 1924 1924
Size: 4904
MluI A'CGCG_T
Cuts at: 4337 4337
Size: 4904
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 545 1139 3575 3759 545
Size: 594 2436 184 1690
Fragments arranged by size:
2436 1690 594 184
MscI TGG'CCA
Cuts at: 1892 2665 1892
Size: 773 4131
MslI CAynn'nnrTG
Cuts at: 911 1372 2893 3020 911
Size: 461 1521 127 2795
Fragments arranged by size:
2795 1521 461 127
MspA1I CmG'CkG
Cuts at: 162 1329 2015 2329 2689 3702 3947 162
Size: 1167 686 314 360 1013 245 1119
Fragments arranged by size:
1167 1119 1013 686 360 314 245
NaeI GCC'GGC
Cuts at: 3085 3085
Size: 4904
NarI GG'CG_CC
Cuts at: 2335 2583 2335
Size: 248 4656
NcoI C'CATG_G
Cuts at: 107 107
Size: 4904
NgoMIV G'CCGG_C
Cuts at: 3083 3083
Size: 4904
NsiI A_TGCA'T
Cuts at: 674 1883 674
Size: 1209 3695
NspI r_CATG'y
Cuts at: 676 1876 1957 2457 3364 676
Size: 1200 81 500 907 2216
Fragments arranged by size:
2216 1200 907 500 81
PciI A'CATG_T
Cuts at: 3360 3360
Size: 4904
PfoI T'CCnGG_A
Cuts at: 146 146
Size: 4904
PmeI GTTT'AAAC
Cuts at: 1903 1903
Size: 4904
PmlI CAC'GTG
Cuts at: 825 825
Size: 4904
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 4043
Size: 32 4872
PsiI TTA'TAA
Cuts at: 644 1253 644
Size: 609 4295
PstI C_TGCA'G
Cuts at: 1340 1951 2636 1340
Size: 611 685 3608
Fragments arranged by size:
3608 685 611
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4904
PvuII CAG'CTG
Cuts at: 162 1329 2329 2689 162
Size: 1167 1000 360 2377
Fragments arranged by size:
2377 1167 1000 360
RsrII CG'GwC_CG
Cuts at: 3099 3099
Size: 4904
SacI G_AGCT'C
Cuts at: 1918 1918
Size: 4904
SalI G'TCGA_C
Cuts at: 1941 1941
Size: 4904
SapI GCTCTTCn'nnn_
Cuts at: 968 2927 3137 968
Size: 1959 210 2735
Fragments arranged by size:
2735 1959 210
SbfI CC_TGCA'GG
Cuts at: 1951 1951
Size: 4904
ScaI AGT'ACT
Cuts at: 4421 4421
Size: 4904
SfcI C'TryA_G
Cuts at: 33 1336 1947 2632 3625 3816 33
Size: 1303 611 685 993 191 1121
Fragments arranged by size:
1303 1121 993 685 611 191
SfoI GGC'GCC
Cuts at: 2336 2584 2336
Size: 248 4656
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4904
SmaI CCC'GGG
Cuts at: 1926 1926
Size: 4904
SmlI C'TyrA_G
Cuts at: 757 2059 2974 3466 3728 757
Size: 1302 915 492 262 1933
Fragments arranged by size:
1933 1302 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4904
SphI G_CATG'C
Cuts at: 676 1957 2457 676
Size: 1281 500 3123
Fragments arranged by size:
3123 1281 500
SspI AAT'ATT
Cuts at: 1702 1702
Size: 4904
StyI C'CwwG_G
Cuts at: 107 1785 2038 2392 107
Size: 1678 253 354 2619
Fragments arranged by size:
2619 1678 354 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2487 2620 3079 3288 2487
Size: 133 459 209 4103
Fragments arranged by size:
4103 459 209 133
TatI w'GTAC_w
Cuts at: 230 372 4419 230
Size: 142 4047 715
Fragments arranged by size:
4047 715 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 900 932 1015 1047 1440 1472 4421 4453 900
Size: 32 83 32 393 32 2949 32 1351
Fragments arranged by size:
2949 1351 393 83 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2701 2701
Size: 4904
XbaI T'CTAG_A
Cuts at: 225 1935 225
Size: 1710 3194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 558 723 2399 558
Size: 165 1676 3063
Fragments arranged by size:
3063 1676 165
XmaI C'CCGG_G
Cuts at: 1924 1924
Size: 4904
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PfoI PmeI PmlI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BmgBI BmtI BsiWI BsmBI BssHII
BstEII BstZ17I CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI PacI PasI PflMI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI