Restriction map for
pF1KB6148
(Circular) (Six-base) MAPSORT of: pF1KB6148.seq Check: 685 from: 1 to: 5033
pF1KB6148 5033 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 2071 2071
Size: 5033
Acc65I G'GTAC_C
Cuts at: 2049 2049
Size: 5033
AclI AA'CG_TT
Cuts at: 4873 4873
Size: 5033
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 775 1652 2858 3290 4037 4305 775
Size: 877 1206 432 747 268 1503
Fragments arranged by size:
1503 1206 877 747 432 268
AfeI AGC'GCT
Cuts at: 148 148
Size: 5033
AflII C'TTAA_G
Cuts at: 1576 1576
Size: 5033
AflIII A'CryG_T
Cuts at: 2385 3489 4466 2385
Size: 1104 977 2952
Fragments arranged by size:
2952 1104 977
AgeI A'CCGG_T
Cuts at: 3421 3421
Size: 5033
AhdI GACnn_n'nnGTC
Cuts at: 948 948
Size: 5033
AlwNI CAG_nnn'CTG
Cuts at: 640 2338 3905 640
Size: 1698 1567 1768
Fragments arranged by size:
1768 1698 1567
ApaLI G'TGCA_C
Cuts at: 3803 3803
Size: 5033
ApoI r'AATT_y
Cuts at: 255 311 602 861 1284 1620 1996 2037 4386
Size: 56 291 259 423 336 376 41 2349
Cuts at: 4386 4642 255
Size: 256 646
Fragments arranged by size:
2349 646 423 376 336 291 259 256
56 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 5033
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5033
AvaI C'yCGr_G
Cuts at: 232 2053 232
Size: 1821 3212
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1350 1383 1350
Size: 33 5000
BamHI G'GATC_C
Cuts at: 2058 2058
Size: 5033
BanI G'GyrC_C
Cuts at: 1003 2049 2463 2711 2746 1003
Size: 1046 414 248 35 3290
Fragments arranged by size:
3290 1046 414 248 35
BanII G_rGCy'C
Cuts at: 2047 3077 2047
Size: 1030 4003
BbeI G_GCGC'C
Cuts at: 2467 2715 2467
Size: 248 4785
BciVI GTATCCnnnnn_n'
Cuts at: 922 2931 3692 5033 922
Size: 2009 761 1341 922
Fragments arranged by size:
2009 1341 922 761
BclI T'GATC_A
Cuts at: 2553 2553
Size: 5033
BglI GCCn_nnn'nGGC
Cuts at: 112 757 112
Size: 645 4388
BglII A'GATC_T
Cuts at: 1572 2548 4585 1572
Size: 976 2037 2020
Fragments arranged by size:
2037 2020 976
BlpI GC'TnA_GC
Cuts at: 2145 2145
Size: 5033
Bme1580I G_kGCm'C
Cuts at: 2658 2751 3807 2658
Size: 93 1056 3884
Fragments arranged by size:
3884 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2245 2326 2661 4805 2245
Size: 81 335 2144 2473
Fragments arranged by size:
2473 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3400 3400
Size: 5033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 449 775 1295 1596 1832 405
Size: 44 326 520 301 236 3606
Fragments arranged by size:
3606 520 326 301 236 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 602 1313 2209 3088 3580 3878 602
Size: 711 896 879 492 298 1757
Fragments arranged by size:
1757 896 879 711 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4737 4737
Size: 5033
BsaAI yAC'GTr
Cuts at: 2386 2386
Size: 5033
BsaBI GATnn'nnATC
Cuts at: 6 2547 4584 6
Size: 2541 2037 455
Fragments arranged by size:
2541 2037 455
BsaHI Gr'CG_yC
Cuts at: 2464 2712 3414 4910 2464
Size: 248 702 1496 2587
Fragments arranged by size:
2587 1496 702 248
BsaWI w'CCGG_w
Cuts at: 771 2222 2743 3421 3695 3842 4253 771
Size: 1451 521 678 274 147 411 1551
Fragments arranged by size:
1551 1451 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 808 838 1282 1312 808
Size: 30 444 30 4529
Fragments arranged by size:
4529 444 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 154 547 1046 1262 1646 154
Size: 393 499 216 384 3541
Fragments arranged by size:
3541 499 393 384 216
BseYI C'CCAG_C
Cuts at: 171 437 2458 3216 3793 4289 171
Size: 266 2021 758 577 496 915
Fragments arranged by size:
2021 915 758 577 496 266
BsiEI CG_ry'CG
Cuts at: 104 2575 2621 3829 104
Size: 2471 46 1208 1308
Fragments arranged by size:
2471 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 968 2047 2825 3015 3807 968
Size: 1079 778 190 792 2194
Fragments arranged by size:
2194 1079 792 778 190
BsmI GAATG_Cn'
Cuts at: 999 999
Size: 5033
BsmBI CGTCTCn'nnnn_
Cuts at: 208 992 1034 1719 208
Size: 784 42 685 3522
Fragments arranged by size:
3522 784 685 42
Bsp1286I G_dGCh'C
Cuts at: 968 2047 2658 2751 2825 3015 3077 3807 968
Size: 1079 611 93 74 190 62 730 2194
Fragments arranged by size:
2194 1079 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4253 4253
Size: 5033
BspHI T'CATG_A
Cuts at: 1875 4209 1875
Size: 2334 2699
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2083 2297 2599 2980 2083
Size: 214 302 381 4136
Fragments arranged by size:
4136 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3325 3379 4863 5028 50
Size: 3275 54 1484 165 55
Fragments arranged by size:
3275 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2945 2945
Size: 5033
BsrFI r'CCGG_y
Cuts at: 3031 3212 3421 3031
Size: 181 209 4643
Fragments arranged by size:
4643 209 181
BssSI C'ACGA_G
Cuts at: 509 3304 3662 509
Size: 2795 358 1880
Fragments arranged by size:
2795 1880 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2410 2410
Size: 5033
BstBI TT'CG_AA
Cuts at: 684 3394 684
Size: 2710 2323
BstXI CCAn_nnnn'nTGG
Cuts at: 799 799
Size: 5033
BstYI r'GATC_y
Cuts at: 1 1572 2058 2525 2548 2883 3129 4141 4227
Size: 1571 486 467 23 335 246 1012 86
Cuts at: 4227 4239 4585 1
Size: 12 346 449
Fragments arranged by size:
1571 1012 486 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 386 386
Size: 5033
BtgI C'CryG_G
Cuts at: 107 297 1658 107
Size: 190 1361 3482
Fragments arranged by size:
3482 1361 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2983 3164 3327 2983
Size: 181 163 4689
Fragments arranged by size:
4689 181 163
BtsI GCAGTG_nn'
Cuts at: 1649 2276 1649
Size: 627 4406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 555 590 555
Size: 35 4998
DraI TTT'AAA
Cuts at: 903 2032 903
Size: 1129 3904
DrdI GACnn_nn'nnGTC
Cuts at: 2739 3597 2739
Size: 858 4175
EaeI y'GGCC_r
Cuts at: 2618 2792 3183 3210 4555 2618
Size: 174 391 27 1345 3096
Fragments arranged by size:
3096 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2618 2618
Size: 5033
EarI CTCTTCn'nnn_
Cuts at: 1580 3056 3266 1580
Size: 1476 210 3347
Fragments arranged by size:
3347 1476 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3241 3551 3697 4843 3241
Size: 310 146 1146 3431
Fragments arranged by size:
3431 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2045 2045
Size: 5033
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 449 775 1295 1596 1652 1832 2858 3290
Size: 44 326 520 301 56 180 1026 432
Cuts at: 3290 4037 4305 405
Size: 747 268 1133
Fragments arranged by size:
1133 1026 747 520 432 326 301 268
180 56 44
EcoNI CCTnn'n_nnAGG
Cuts at: 136 136
Size: 5033
EcoO109I rG'GnC_Cy
Cuts at: 129 870 2172 129
Size: 741 1302 2990
Fragments arranged by size:
2990 1302 741
EcoRI G'AATT_C
Cuts at: 861 2037 861
Size: 1176 3857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1764 3052 1764
Size: 1288 3745
FspI TGC'GCA
Cuts at: 2814 2814
Size: 5033
HaeII r_GCGC'y
Cuts at: 150 232 1842 2467 2715 3737 4716 150
Size: 82 1610 625 248 1022 979 467
Fragments arranged by size:
1610 1022 979 625 467 248 82
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 263 295 523 555 808 840 934 966 1033
Size: 32 228 32 253 32 94 32 67
Cuts at: 1033 1065 2008 2040 2875 2905 2907 2937 263
Size: 32 943 32 835 30 2 30 2359
Fragments arranged by size:
2359 943 835 253 228 94 67 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 656 1344 2072 656
Size: 688 728 3617
Fragments arranged by size:
3617 728 688
KasI G'GCGC_C
Cuts at: 2463 2711 2463
Size: 248 4785
KpnI G_GTAC'C
Cuts at: 2053 2053
Size: 5033
MfeI C'AATT_G
Cuts at: 444 1673 444
Size: 1229 3804
MluI A'CGCG_T
Cuts at: 4466 4466
Size: 5033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1319 3704 3888 1319
Size: 2385 184 2464
Fragments arranged by size:
2464 2385 184
MscI TGG'CCA
Cuts at: 2794 2794
Size: 5033
MslI CAynn'nnrTG
Cuts at: 3022 3149 3022
Size: 127 4906
MspA1I CmG'CkG
Cuts at: 1200 1239 2144 2458 2818 3831 4076 1200
Size: 39 905 314 360 1013 245 2157
Fragments arranged by size:
2157 1013 905 360 314 245 39
NaeI GCC'GGC
Cuts at: 3214 3214
Size: 5033
NarI GG'CG_CC
Cuts at: 2464 2712 2464
Size: 248 4785
NcoI C'CATG_G
Cuts at: 107 1658 107
Size: 1551 3482
NdeI CA'TA_TG
Cuts at: 582 582
Size: 5033
NgoMIV G'CCGG_C
Cuts at: 3212 3212
Size: 5033
NspI r_CATG'y
Cuts at: 202 1786 2086 2586 3493 202
Size: 1584 300 500 907 1742
Fragments arranged by size:
1742 1584 907 500 300
PasI CC'CwG_GG
Cuts at: 828 828
Size: 5033
PciI A'CATG_T
Cuts at: 3489 3489
Size: 5033
PmeI GTTT'AAAC
Cuts at: 2032 2032
Size: 5033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4172 4204 4172
Size: 32 5001
PpuMI rG'GwC_Cy
Cuts at: 870 870
Size: 5033
PsiI TTA'TAA
Cuts at: 1739 1739
Size: 5033
PstI C_TGCA'G
Cuts at: 1002 1191 2080 2765 1002
Size: 189 889 685 3270
Fragments arranged by size:
3270 889 685 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5033
PvuII CAG'CTG
Cuts at: 2458 2818 2458
Size: 360 4673
RsrII CG'GwC_CG
Cuts at: 3228 3228
Size: 5033
SacI G_AGCT'C
Cuts at: 2047 2047
Size: 5033
SalI G'TCGA_C
Cuts at: 2070 2070
Size: 5033
SapI GCTCTTCn'nnn_
Cuts at: 3056 3266 3056
Size: 210 4823
SbfI CC_TGCA'GG
Cuts at: 2080 2080
Size: 5033
ScaI AGT'ACT
Cuts at: 4550 4550
Size: 5033
SfcI C'TryA_G
Cuts at: 33 998 1187 1799 2076 2761 3754 3945 33
Size: 965 189 612 277 685 993 191 1121
Fragments arranged by size:
1121 993 965 685 612 277 191 189
SfiI GGCCn_nnn'nGGCC
Cuts at: 757 757
Size: 5033
SfoI GGC'GCC
Cuts at: 2465 2713 2465
Size: 248 4785
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5033
SmaI CCC'GGG
Cuts at: 2055 2055
Size: 5033
SmlI C'TyrA_G
Cuts at: 232 617 1328 1576 2188 3103 3595 3857 232
Size: 385 711 248 612 915 492 262 1408
Fragments arranged by size:
1408 915 711 612 492 385 262 248
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5033
SphI G_CATG'C
Cuts at: 1786 2086 2586 1786
Size: 300 500 4233
Fragments arranged by size:
4233 500 300
StuI AGG'CCT
Cuts at: 760 1193 760
Size: 433 4600
StyI C'CwwG_G
Cuts at: 107 288 1658 2167 2521 107
Size: 181 1370 509 354 2619
Fragments arranged by size:
2619 1370 509 354 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2616 2749 3208 3417 2616
Size: 133 459 209 4232
Fragments arranged by size:
4232 459 209 133
TatI w'GTAC_w
Cuts at: 1769 4548 1769
Size: 2779 2254
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1474 1506 4550 4582 1474
Size: 32 3044 32 1925
Fragments arranged by size:
3044 1925 32 32
Tth111I GACn'n_nGTC
Cuts at: 2830 2830
Size: 5033
XbaI T'CTAG_A
Cuts at: 970 2064 970
Size: 1094 3939
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2528 2528
Size: 5033
XhoI C'TCGA_G
Cuts at: 232 232
Size: 5033
XmaI C'CCGG_G
Cuts at: 2053 2053
Size: 5033
XmnI GAAnn'nnTTC
Cuts at: 1955 1955
Size: 5033
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AleI AlfI AloI ApaI AscI AvrII
BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsgI
BsiWI BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
ZraI