Restriction map for
pF1KB6143
(Circular) (Six-base) MAPSORT of: pF1KB6143.seq Check: 379 from: 1 to: 4898
pF1KB6143 4898 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 1936 1936
Size: 4898
Acc65I G'GTAC_C
Cuts at: 1914 1914
Size: 4898
AclI AA'CG_TT
Cuts at: 4738 4738
Size: 4898
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1084 2723 3155 3902 4170 1084
Size: 1639 432 747 268 1812
Fragments arranged by size:
1812 1639 747 432 268
AfeI AGC'GCT
Cuts at: 1384 1384
Size: 4898
AflIII A'CryG_T
Cuts at: 1745 2250 3354 4331 1745
Size: 505 1104 977 2312
Fragments arranged by size:
2312 1104 977 505
AgeI A'CCGG_T
Cuts at: 3286 3286
Size: 4898
AhdI GACnn_n'nnGTC
Cuts at: 429 429
Size: 4898
AlwNI CAG_nnn'CTG
Cuts at: 331 583 2203 3770 331
Size: 252 1620 1567 1459
Fragments arranged by size:
1620 1567 1459 252
ApaI G_GGCC'C
Cuts at: 489 623 1396 489
Size: 134 773 3991
Fragments arranged by size:
3991 773 134
ApaLI G'TGCA_C
Cuts at: 3668 3668
Size: 4898
ApoI r'AATT_y
Cuts at: 281 321 713 727 800 1072 1170 1902 4251
Size: 40 392 14 73 272 98 732 2349
Cuts at: 4251 4507 281
Size: 256 672
Fragments arranged by size:
2349 732 672 392 272 256 98 73
40 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4898
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
AvaI C'yCGr_G
Cuts at: 122 1918 122
Size: 1796 3102
BamHI G'GATC_C
Cuts at: 1923 1923
Size: 4898
BanI G'GyrC_C
Cuts at: 1266 1914 2328 2576 2611 1266
Size: 648 414 248 35 3553
Fragments arranged by size:
3553 648 414 248 35
BanII G_rGCy'C
Cuts at: 489 510 623 1396 1912 2942 489
Size: 21 113 773 516 1030 2445
Fragments arranged by size:
2445 1030 773 516 113 21
BbeI G_GCGC'C
Cuts at: 2332 2580 2332
Size: 248 4650
BbsI GAAGACnn'nnnn_
Cuts at: 614 614
Size: 4898
BbvCI CC'TCA_GC
Cuts at: 1861 1873 1861
Size: 12 4886
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1831 1865 1831
Size: 34 4864
BciVI GTATCCnnnnn_n'
Cuts at: 2796 3557 4898 2796
Size: 761 1341 2796
Fragments arranged by size:
2796 1341 761
BclI T'GATC_A
Cuts at: 2418 2418
Size: 4898
BglI GCCn_nnn'nGGC
Cuts at: 118 118
Size: 4898
BglII A'GATC_T
Cuts at: 1482 1560 2413 4450 1482
Size: 78 853 2037 1930
Fragments arranged by size:
2037 1930 853 78
BlpI GC'TnA_GC
Cuts at: 674 2010 674
Size: 1336 3562
Bme1580I G_kGCm'C
Cuts at: 489 623 1396 2523 2616 3672 489
Size: 134 773 1127 93 1056 1715
Fragments arranged by size:
1715 1127 1056 773 134 93
BmrI ACTGGGnnnn_n'
Cuts at: 284 471 546 609 621 2110 2191 2526 4670
Size: 187 75 63 12 1489 81 335 2144
Cuts at: 4670 284
Size: 512
Fragments arranged by size:
2144 1489 512 335 187 81 75 63
12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3265 3265
Size: 4898
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 524 1840 459
Size: 65 1316 3517
Fragments arranged by size:
3517 1316 65
Bpu10I CC'TnA_GC
Cuts at: 371 1861 1873 371
Size: 1490 12 3396
Fragments arranged by size:
3396 1490 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 2953 3445 3743 2074
Size: 879 492 298 3229
Fragments arranged by size:
3229 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4602 4602
Size: 4898
BsaAI yAC'GTr
Cuts at: 2251 2251
Size: 4898
BsaBI GATnn'nnATC
Cuts at: 6 2412 4449 6
Size: 2406 2037 455
Fragments arranged by size:
2406 2037 455
BsaHI Gr'CG_yC
Cuts at: 2329 2577 3279 4775 2329
Size: 248 702 1496 2452
Fragments arranged by size:
2452 1496 702 248
BsaWI w'CCGG_w
Cuts at: 204 1781 2087 2608 3286 3560 3707 4118 204
Size: 1577 306 521 678 274 147 411 984
Fragments arranged by size:
1577 984 678 521 411 306 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 510 540 510
Size: 30 4868
BseYI C'CCAG_C
Cuts at: 479 488 2323 3081 3658 4154 479
Size: 9 1835 758 577 496 1223
Fragments arranged by size:
1835 1223 758 577 496 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1371 1371
Size: 4898
BsiEI CG_ry'CG
Cuts at: 104 2440 2486 3694 104
Size: 2336 46 1208 1308
Fragments arranged by size:
2336 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1376 1912 2690 2880 3672 1376
Size: 536 778 190 792 2602
Fragments arranged by size:
2602 792 778 536 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1000 1000
Size: 4898
Bsp1286I G_dGCh'C
Cuts at: 489 510 623 1376 1396 1912 2523 2616 2690
Size: 21 113 753 20 516 611 93 74
Cuts at: 2690 2880 2942 3672 489
Size: 190 62 730 1715
Fragments arranged by size:
1715 753 730 611 516 190 113 93
74 62 21 20
BspEI T'CCGG_A
Cuts at: 204 4118 204
Size: 3914 984
BspHI T'CATG_A
Cuts at: 1238 4074 1238
Size: 2836 2062
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1948 2162 2464 2845 1948
Size: 214 302 381 4001
Fragments arranged by size:
4001 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3190 3244 4728 4893 50
Size: 3140 54 1484 165 55
Fragments arranged by size:
3140 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 426 2810 426
Size: 2384 2514
BsrFI r'CCGG_y
Cuts at: 2896 3077 3286 2896
Size: 181 209 4508
Fragments arranged by size:
4508 209 181
BssSI C'ACGA_G
Cuts at: 1537 1588 3169 3527 1537
Size: 51 1581 358 2908
Fragments arranged by size:
2908 1581 358 51
BstAPI GCAn_nnn'nTGC
Cuts at: 1830 2275 1830
Size: 445 4453
BstBI TT'CG_AA
Cuts at: 3259 3259
Size: 4898
BstXI CCAn_nnnn'nTGG
Cuts at: 1332 1539 1332
Size: 207 4691
BstYI r'GATC_y
Cuts at: 1 873 1482 1560 1784 1923 2390 2413 2748
Size: 872 609 78 224 139 467 23 335
Cuts at: 2748 2994 4006 4092 4104 4450 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 872 609 467 449 346 335 246
224 139 86 78 23 12
BtgI C'CryG_G
Cuts at: 107 1253 1331 107
Size: 1146 78 3674
Fragments arranged by size:
3674 1146 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2848 3029 3192 2848
Size: 181 163 4554
Fragments arranged by size:
4554 181 163
BtsI GCAGTG_nn'
Cuts at: 420 542 2141 420
Size: 122 1599 3177
Fragments arranged by size:
3177 1599 122
ClaI AT'CG_AT
Cuts at: 948 948
Size: 4898
DraI TTT'AAA
Cuts at: 1897 1897
Size: 4898
DrdI GACnn_nn'nnGTC
Cuts at: 1048 2604 3462 1048
Size: 1556 858 2484
Fragments arranged by size:
2484 1556 858
EaeI y'GGCC_r
Cuts at: 110 552 770 1442 2483 2657 3048 3075 4420
Size: 442 218 672 1041 174 391 27 1345
Cuts at: 4420 110
Size: 588
Fragments arranged by size:
1345 1041 672 588 442 391 218 174
27
EagI C'GGCC_G
Cuts at: 2483 2483
Size: 4898
EarI CTCTTCn'nnn_
Cuts at: 636 1040 1061 1717 2921 3131 636
Size: 404 21 656 1204 210 2403
Fragments arranged by size:
2403 1204 656 404 210 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1798 3106 3416 3562 4708 1798
Size: 1308 310 146 1146 1988
Fragments arranged by size:
1988 1308 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1910 1910
Size: 4898
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 524 1084 1840 2723 3155 3902 4170 459
Size: 65 560 756 883 432 747 268 1187
Fragments arranged by size:
1187 883 756 747 560 432 268 65
EcoO109I rG'GnC_Cy
Cuts at: 485 1869 2037 485
Size: 1384 168 3346
Fragments arranged by size:
3346 1384 168
EcoRI G'AATT_C
Cuts at: 1902 1902
Size: 4898
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 361 1621 2917 361
Size: 1260 1296 2342
Fragments arranged by size:
2342 1296 1260
FspI TGC'GCA
Cuts at: 1087 2679 1087
Size: 1592 3306
FspAI rTGC'GCAy
Cuts at: 1087 1087
Size: 4898
HaeII r_GCGC'y
Cuts at: 674 1386 2332 2580 3602 4581 674
Size: 712 946 248 1022 979 991
Fragments arranged by size:
1022 991 979 946 712 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 173 205 340 372 415 447 637 669 1718
Size: 32 135 32 43 32 190 32 1049
Cuts at: 1718 1750 2740 2770 2772 2802 173
Size: 32 990 30 2 30 2269
Fragments arranged by size:
2269 1049 990 190 135 43 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1937 1937
Size: 4898
KasI G'GCGC_C
Cuts at: 2328 2576 2328
Size: 248 4650
KpnI G_GTAC'C
Cuts at: 1918 1918
Size: 4898
MluI A'CGCG_T
Cuts at: 1745 4331 1745
Size: 2586 2312
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 795 3569 3753 795
Size: 2774 184 1940
Fragments arranged by size:
2774 1940 184
MscI TGG'CCA
Cuts at: 112 772 1444 2659 112
Size: 660 672 1215 2351
Fragments arranged by size:
2351 1215 672 660
MslI CAynn'nnrTG
Cuts at: 2887 3014 2887
Size: 127 4771
MspA1I CmG'CkG
Cuts at: 658 2009 2323 2683 3696 3941 658
Size: 1351 314 360 1013 245 1615
Fragments arranged by size:
1615 1351 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3079 3079
Size: 4898
NarI GG'CG_CC
Cuts at: 2329 2577 2329
Size: 248 4650
NcoI C'CATG_G
Cuts at: 107 1253 1331 107
Size: 1146 78 3674
Fragments arranged by size:
3674 1146 78
NgoMIV G'CCGG_C
Cuts at: 3077 3077
Size: 4898
NspI r_CATG'y
Cuts at: 1951 2451 3358 1951
Size: 500 907 3491
Fragments arranged by size:
3491 907 500
PciI A'CATG_T
Cuts at: 3354 3354
Size: 4898
PflMI CCAn_nnn'nTGG
Cuts at: 536 583 536
Size: 47 4851
PfoI T'CCnGG_A
Cuts at: 732 732
Size: 4898
PmeI GTTT'AAAC
Cuts at: 1897 1897
Size: 4898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 245 277 4037 4069 245
Size: 32 3760 32 1074
Fragments arranged by size:
3760 1074 32 32
PspOMI G'GGCC_C
Cuts at: 485 619 1392 485
Size: 134 773 3991
Fragments arranged by size:
3991 773 134
PstI C_TGCA'G
Cuts at: 1945 2630 1945
Size: 685 4213
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4898
PvuII CAG'CTG
Cuts at: 658 2323 2683 658
Size: 1665 360 2873
Fragments arranged by size:
2873 1665 360
RsrII CG'GwC_CG
Cuts at: 3093 3093
Size: 4898
SacI G_AGCT'C
Cuts at: 1912 1912
Size: 4898
SalI G'TCGA_C
Cuts at: 1935 1935
Size: 4898
SapI GCTCTTCn'nnn_
Cuts at: 2921 3131 2921
Size: 210 4688
SbfI CC_TGCA'GG
Cuts at: 1945 1945
Size: 4898
ScaI AGT'ACT
Cuts at: 4415 4415
Size: 4898
SexAI A'CCwGG_T
Cuts at: 404 404
Size: 4898
SfcI C'TryA_G
Cuts at: 33 1941 2626 3619 3810 33
Size: 1908 685 993 191 1121
Fragments arranged by size:
1908 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2330 2578 2330
Size: 248 4650
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
SmaI CCC'GGG
Cuts at: 1920 1920
Size: 4898
SmlI C'TyrA_G
Cuts at: 2053 2968 3460 3722 2053
Size: 915 492 262 3229
Fragments arranged by size:
3229 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1674 67
Size: 1607 3291
SphI G_CATG'C
Cuts at: 1951 2451 1951
Size: 500 4398
StuI AGG'CCT
Cuts at: 565 1105 1429 565
Size: 540 324 4034
Fragments arranged by size:
4034 540 324
StyI C'CwwG_G
Cuts at: 107 116 570 1253 1325 1331 1403 2032 2386
Size: 9 454 683 72 6 72 629 354
Cuts at: 2386 107
Size: 2619
Fragments arranged by size:
2619 683 629 454 354 72 72 9
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2481 2614 3073 3282 2481
Size: 133 459 209 4097
Fragments arranged by size:
4097 459 209 133
TatI w'GTAC_w
Cuts at: 4413 4413
Size: 4898
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 311 343 510 542 4415 4447 311
Size: 32 167 32 3873 32 762
Fragments arranged by size:
3873 762 167 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2695 2695
Size: 4898
XbaI T'CTAG_A
Cuts at: 1929 1929
Size: 4898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 412 2393 412
Size: 1981 2917
XmaI C'CCGG_G
Cuts at: 1918 1918
Size: 4898
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BseRI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV
FseI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI