Restriction map for
pF1KB6142
(Circular) (Six-base) MAPSORT of: pF1KB6142.seq Check: 5394 from: 1 to: 5027
pF1KB6142 5027 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1444 1444
Size: 5027
AccI GT'mk_AC
Cuts at: 2065 2065
Size: 5027
Acc65I G'GTAC_C
Cuts at: 2043 2043
Size: 5027
AclI AA'CG_TT
Cuts at: 2021 4867 2021
Size: 2846 2181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 575 657 1155 1879 2852 3284 4031 4299
Size: 258 82 498 724 973 432 747 268
Cuts at: 4299 317
Size: 1045
Fragments arranged by size:
1045 973 747 724 498 432 268 258
82
AflIII A'CryG_T
Cuts at: 1019 1781 2379 3483 4460 1019
Size: 762 598 1104 977 1586
Fragments arranged by size:
1586 1104 977 762 598
AgeI A'CCGG_T
Cuts at: 189 3415 189
Size: 3226 1801
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 841 1579 841
Size: 738 4289
AlwNI CAG_nnn'CTG
Cuts at: 372 1341 2332 3899 372
Size: 969 991 1567 1500
Fragments arranged by size:
1567 1500 991 969
ApaI G_GGCC'C
Cuts at: 919 950 919
Size: 31 4996
ApaLI G'TGCA_C
Cuts at: 3797 3797
Size: 5027
ApoI r'AATT_y
Cuts at: 1417 1602 2031 4380 4636 1417
Size: 185 429 2349 256 1808
Fragments arranged by size:
2349 1808 429 256 185
AseI AT'TA_AT
Cuts at: 20 20
Size: 5027
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
AvaI C'yCGr_G
Cuts at: 950 1195 1270 1799 2047 950
Size: 245 75 529 248 3930
Fragments arranged by size:
3930 529 248 245 75
BamHI G'GATC_C
Cuts at: 2052 2052
Size: 5027
BanI G'GyrC_C
Cuts at: 1456 1696 1804 2043 2457 2705 2740 1456
Size: 240 108 239 414 248 35 3743
Fragments arranged by size:
3743 414 248 240 239 108 35
BanII G_rGCy'C
Cuts at: 919 950 959 2041 3071 919
Size: 31 9 1082 1030 2875
Fragments arranged by size:
2875 1082 1030 31 9
BbeI G_GCGC'C
Cuts at: 2461 2709 2461
Size: 248 4779
BbsI GAAGACnn'nnnn_
Cuts at: 186 351 1799 186
Size: 165 1448 3414
Fragments arranged by size:
3414 1448 165
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1389 1423 1389
Size: 34 4993
BciVI GTATCCnnnnn_n'
Cuts at: 565 2925 3686 5027 565
Size: 2360 761 1341 565
Fragments arranged by size:
2360 1341 761 565
BclI T'GATC_A
Cuts at: 503 2547 503
Size: 2044 2983
BglI GCCn_nnn'nGGC
Cuts at: 1001 1207 1273 1001
Size: 206 66 4755
Fragments arranged by size:
4755 206 66
BglII A'GATC_T
Cuts at: 2542 4579 2542
Size: 2037 2990
BlpI GC'TnA_GC
Cuts at: 182 1714 2139 182
Size: 1532 425 3070
Fragments arranged by size:
3070 1532 425
Bme1580I G_kGCm'C
Cuts at: 919 950 1699 1899 2652 2745 3801 919
Size: 31 749 200 753 93 1056 2145
Fragments arranged by size:
2145 1056 753 749 200 93 31
BmrI ACTGGGnnnn_n'
Cuts at: 123 521 2239 2320 2655 4799 123
Size: 398 1718 81 335 2144 351
Fragments arranged by size:
2144 1718 398 351 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 882 1164 3394 882
Size: 282 2230 2515
Fragments arranged by size:
2515 2230 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1062 1321 1639 1837 1984 394
Size: 668 259 318 198 147 3437
Fragments arranged by size:
3437 668 318 259 198 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2203 3082 3574 3872 2203
Size: 879 492 298 3358
Fragments arranged by size:
3358 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 374 1658 4731 374
Size: 1284 3073 670
Fragments arranged by size:
3073 1284 670
BsaAI yAC'GTr
Cuts at: 1020 1784 2380 1020
Size: 764 596 3667
Fragments arranged by size:
3667 764 596
BsaBI GATnn'nnATC
Cuts at: 6 460 895 2541 4578 6
Size: 454 435 1646 2037 455
Fragments arranged by size:
2037 1646 455 454 435
BsaHI Gr'CG_yC
Cuts at: 1583 1722 1796 2458 2706 3408 4904 1583
Size: 139 74 662 248 702 1496 1706
Fragments arranged by size:
1706 1496 702 662 248 139 74
BsaWI w'CCGG_w
Cuts at: 189 821 2216 2737 3415 3689 3836 4247 189
Size: 632 1395 521 678 274 147 411 969
Fragments arranged by size:
1395 969 678 632 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 625 655 776 806 1508 1538 625
Size: 30 121 30 702 30 4114
Fragments arranged by size:
4114 702 121 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 855 1676 1972 855
Size: 821 296 3910
Fragments arranged by size:
3910 821 296
BseYI C'CCAG_C
Cuts at: 567 911 1821 2452 3210 3787 4283 567
Size: 344 910 631 758 577 496 1311
Fragments arranged by size:
1311 910 758 631 577 496 344
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 764 1218 1546 764
Size: 454 328 4245
Fragments arranged by size:
4245 454 328
BsiEI CG_ry'CG
Cuts at: 104 143 190 2569 2615 3823 104
Size: 39 47 2379 46 1208 1308
Fragments arranged by size:
2379 1308 1208 47 46 39
BsiHKAI G_wGCw'C
Cuts at: 959 1352 2041 2819 3009 3801 959
Size: 393 689 778 190 792 2185
Fragments arranged by size:
2185 792 778 689 393 190
BsmI GAATG_Cn'
Cuts at: 1245 1245
Size: 5027
Bsp1286I G_dGCh'C
Cuts at: 919 950 959 1352 1699 1899 2041 2652 2745
Size: 31 9 393 347 200 142 611 93
Cuts at: 2745 2819 3009 3071 3801 919
Size: 74 190 62 730 2145
Fragments arranged by size:
2145 730 611 393 347 200 190 142
93 74 62 31 9
BspEI T'CCGG_A
Cuts at: 821 4247 821
Size: 3426 1601
BspHI T'CATG_A
Cuts at: 842 1769 4203 842
Size: 927 2434 1666
Fragments arranged by size:
2434 1666 927
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1444 2077 2291 2593 2974 1444
Size: 633 214 302 381 3497
Fragments arranged by size:
3497 633 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3319 3373 4857 5022 50
Size: 3269 54 1484 165 55
Fragments arranged by size:
3269 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1407 2939 1407
Size: 1532 3495
BsrFI r'CCGG_y
Cuts at: 189 1201 1718 3025 3206 3415 189
Size: 1012 517 1307 181 209 1801
Fragments arranged by size:
1801 1307 1012 517 209 181
BssSI C'ACGA_G
Cuts at: 3298 3656 3298
Size: 358 4669
BstAPI GCAn_nnn'nTGC
Cuts at: 2404 2404
Size: 5027
BstBI TT'CG_AA
Cuts at: 3388 3388
Size: 5027
BstYI r'GATC_y
Cuts at: 1 2052 2519 2542 2877 3123 4135 4221 4233
Size: 2051 467 23 335 246 1012 86 12
Cuts at: 4233 4579 1
Size: 346 449
Fragments arranged by size:
2051 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 729 971 996 1460 1985 729
Size: 242 25 464 525 3771
Fragments arranged by size:
3771 525 464 242 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2977 3158 3321 2977
Size: 181 163 4683
Fragments arranged by size:
4683 181 163
BtsI GCAGTG_nn'
Cuts at: 499 2270 499
Size: 1771 3256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 762 797 762
Size: 35 4992
DraI TTT'AAA
Cuts at: 2026 2026
Size: 5027
DraIII CAC_nnn'GTG
Cuts at: 1512 1512
Size: 5027
DrdI GACnn_nn'nnGTC
Cuts at: 2733 3591 2733
Size: 858 4169
EaeI y'GGCC_r
Cuts at: 140 974 1940 2612 2786 3177 3204 4549 140
Size: 834 966 672 174 391 27 1345 618
Fragments arranged by size:
1345 966 834 672 618 391 174 27
EagI C'GGCC_G
Cuts at: 140 2612 140
Size: 2472 2555
EarI CTCTTCn'nnn_
Cuts at: 160 1155 1653 1964 3050 3260 160
Size: 995 498 311 1086 210 1927
Fragments arranged by size:
1927 1086 995 498 311 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3235 3545 3691 4837 3235
Size: 310 146 1146 3425
Fragments arranged by size:
3425 1146 310 146
EcoICRI GAG'CTC
Cuts at: 957 2039 957
Size: 1082 3945
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 394 575 657 1062 1155 1321 1639 1837
Size: 77 181 82 405 93 166 318 198
Cuts at: 1837 1879 1984 2852 3284 4031 4299 317
Size: 42 105 868 432 747 268 1045
Fragments arranged by size:
1045 868 747 432 405 318 268 198
181 166 105 93 82 77 42
EcoNI CCTnn'n_nnAGG
Cuts at: 331 331
Size: 5027
EcoO109I rG'GnC_Cy
Cuts at: 734 915 916 947 1443 1530 2166 734
Size: 181 1 31 496 87 636 3595
Fragments arranged by size:
3595 636 496 181 87 31 1
EcoRI G'AATT_C
Cuts at: 2031 2031
Size: 5027
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 306 1767 3046 306
Size: 1461 1279 2287
Fragments arranged by size:
2287 1461 1279
FspI TGC'GCA
Cuts at: 2808 2808
Size: 5027
HaeII r_GCGC'y
Cuts at: 908 1841 2461 2709 3731 4710 908
Size: 933 620 248 1022 979 1225
Fragments arranged by size:
1225 1022 979 933 620 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 606 625 638 657 1707 1723 1739 1755 1915
Size: 19 13 19 1050 16 16 16 160
Cuts at: 1915 1947 2869 2899 2901 2931 606
Size: 32 922 30 2 30 2702
Fragments arranged by size:
2702 1050 922 160 32 30 30 19
19 16 16 16 13 2
HincII GTy'rAC
Cuts at: 1647 2066 1647
Size: 419 4608
HindIII A'AGCT_T
Cuts at: 283 283
Size: 5027
KasI G'GCGC_C
Cuts at: 2457 2705 2457
Size: 248 4779
KpnI G_GTAC'C
Cuts at: 2047 2047
Size: 5027
MluI A'CGCG_T
Cuts at: 4460 4460
Size: 5027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 668 768 3698 3882 668
Size: 100 2930 184 1813
Fragments arranged by size:
2930 1813 184 100
MscI TGG'CCA
Cuts at: 1942 2788 1942
Size: 846 4181
MslI CAynn'nnrTG
Cuts at: 466 823 1018 1678 3016 3143 466
Size: 357 195 660 1338 127 2350
Fragments arranged by size:
2350 1338 660 357 195 127
MspA1I CmG'CkG
Cuts at: 372 888 911 998 1264 1279 2138 2452 2812
Size: 516 23 87 266 15 859 314 360
Cuts at: 2812 3825 4070 372
Size: 1013 245 1329
Fragments arranged by size:
1329 1013 859 516 360 314 266 245
87 23 15
NaeI GCC'GGC
Cuts at: 1720 3208 1720
Size: 1488 3539
NarI GG'CG_CC
Cuts at: 2458 2706 2458
Size: 248 4779
NcoI C'CATG_G
Cuts at: 971 1460 971
Size: 489 4538
NdeI CA'TA_TG
Cuts at: 878 878
Size: 5027
NgoMIV G'CCGG_C
Cuts at: 1718 3206 1718
Size: 1488 3539
NotI GC'GGCC_GC
Cuts at: 140 140
Size: 5027
NspI r_CATG'y
Cuts at: 1086 1335 1627 2080 2580 3487 1086
Size: 249 292 453 500 907 2626
Fragments arranged by size:
2626 907 500 453 292 249
PasI CC'CwG_GG
Cuts at: 386 386
Size: 5027
PciI A'CATG_T
Cuts at: 3483 3483
Size: 5027
PflMI CCAn_nnn'nTGG
Cuts at: 1085 1512 1552 1085
Size: 427 40 4560
Fragments arranged by size:
4560 427 40
PmeI GTTT'AAAC
Cuts at: 2026 2026
Size: 5027
PmlI CAC'GTG
Cuts at: 1784 1784
Size: 5027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4166 4198 4166
Size: 32 4995
PpuMI rG'GwC_Cy
Cuts at: 734 1443 1530 734
Size: 709 87 4231
Fragments arranged by size:
4231 709 87
PspOMI G'GGCC_C
Cuts at: 915 946 915
Size: 31 4996
PstI C_TGCA'G
Cuts at: 968 1263 2074 2759 968
Size: 295 811 685 3236
Fragments arranged by size:
3236 811 685 295
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5027
PvuII CAG'CTG
Cuts at: 372 888 911 1264 1279 2452 2812 372
Size: 516 23 353 15 1173 360 2587
Fragments arranged by size:
2587 1173 516 360 353 23 15
RsrII CG'GwC_CG
Cuts at: 3222 3222
Size: 5027
SacI G_AGCT'C
Cuts at: 959 2041 959
Size: 1082 3945
SacII CC_GC'GG
Cuts at: 999 999
Size: 5027
SalI G'TCGA_C
Cuts at: 2064 2064
Size: 5027
SanDI GG'GwC_CC
Cuts at: 734 734
Size: 5027
SapI GCTCTTCn'nnn_
Cuts at: 1155 1964 3050 3260 1155
Size: 809 1086 210 2922
Fragments arranged by size:
2922 1086 809 210
SbfI CC_TGCA'GG
Cuts at: 2074 2074
Size: 5027
ScaI AGT'ACT
Cuts at: 4544 4544
Size: 5027
SexAI A'CCwGG_T
Cuts at: 1910 1910
Size: 5027
SfcI C'TryA_G
Cuts at: 33 900 964 1259 2070 2755 3748 3939 33
Size: 867 64 295 811 685 993 191 1121
Fragments arranged by size:
1121 993 867 811 685 295 191 64
SfoI GGC'GCC
Cuts at: 2459 2707 2459
Size: 248 4779
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
SmaI CCC'GGG
Cuts at: 952 2049 952
Size: 1097 3930
SmlI C'TyrA_G
Cuts at: 2182 3097 3589 3851 2182
Size: 915 492 262 3358
Fragments arranged by size:
3358 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5027
SphI G_CATG'C
Cuts at: 2080 2580 2080
Size: 500 4527
StuI AGG'CCT
Cuts at: 862 862
Size: 5027
StyI C'CwwG_G
Cuts at: 806 971 1157 1460 1726 1742 2161 2515 806
Size: 165 186 303 266 16 419 354 3318
Fragments arranged by size:
3318 419 354 303 266 186 165 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 437 2610 2743 3202 3411 437
Size: 2173 133 459 209 2053
Fragments arranged by size:
2173 2053 459 209 133
TatI w'GTAC_w
Cuts at: 4542 4542
Size: 5027
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 726 758 1508 1540 4544 4576 726
Size: 32 750 32 3004 32 1177
Fragments arranged by size:
3004 1177 750 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1381 2824 1381
Size: 1443 3584
XbaI T'CTAG_A
Cuts at: 2058 2058
Size: 5027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 393 600 1467 1557 2522 393
Size: 207 867 90 965 2898
Fragments arranged by size:
2898 965 867 207 90
XmaI C'CCGG_G
Cuts at: 950 2047 950
Size: 1097 3930
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI EcoRV
FseI FspAI HpaI MfeI NheI NruI NsiI PacI
PfoI PshAI PsiI PspXI PsrI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI