Restriction map for
pF1KB6133
(Circular) (Six-base) MAPSORT of: pF1KB6133.seq Check: 7988 from: 1 to: 4883
pF1KB6133 4883 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AccI GT'mk_AC
Cuts at: 1921 1921
Size: 4883
Acc65I G'GTAC_C
Cuts at: 1899 1899
Size: 4883
AclI AA'CG_TT
Cuts at: 4723 4723
Size: 4883
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 339 952 1749 2708 3140 3887 4155 141
Size: 198 613 797 959 432 747 268 869
Fragments arranged by size:
959 869 797 747 613 432 268 198
AflIII A'CryG_T
Cuts at: 2235 3339 4316 2235
Size: 1104 977 2802
Fragments arranged by size:
2802 1104 977
AgeI A'CCGG_T
Cuts at: 362 3271 362
Size: 2909 1974
AleI CACnn'nnGTG
Cuts at: 208 1072 208
Size: 864 4019
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 745 777 745
Size: 32 4851
AlwNI CAG_nnn'CTG
Cuts at: 750 855 1293 2188 3755 750
Size: 105 438 895 1567 1878
Fragments arranged by size:
1878 1567 895 438 105
ApaLI G'TGCA_C
Cuts at: 3653 3653
Size: 4883
ApoI r'AATT_y
Cuts at: 1834 1887 4236 4492 1834
Size: 53 2349 256 2225
Fragments arranged by size:
2349 2225 256 53
AseI AT'TA_AT
Cuts at: 20 20
Size: 4883
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4883
AvaI C'yCGr_G
Cuts at: 1903 1903
Size: 4883
AvrII C'CTAG_G
Cuts at: 1731 1731
Size: 4883
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 885 918 885
Size: 33 4850
BamHI G'GATC_C
Cuts at: 1908 1908
Size: 4883
BanI G'GyrC_C
Cuts at: 406 727 1899 2313 2561 2596 406
Size: 321 1172 414 248 35 2693
Fragments arranged by size:
2693 1172 414 321 248 35
BanII G_rGCy'C
Cuts at: 319 458 1475 1745 1759 1897 2927 319
Size: 139 1017 270 14 138 1030 2275
Fragments arranged by size:
2275 1030 1017 270 139 138 14
BbeI G_GCGC'C
Cuts at: 2317 2565 2317
Size: 248 4635
BbsI GAAGACnn'nnnn_
Cuts at: 869 869
Size: 4883
BbvCI CC'TCA_GC
Cuts at: 1127 1127
Size: 4883
BciVI GTATCCnnnnn_n'
Cuts at: 535 2781 3542 4883 535
Size: 2246 761 1341 535
Fragments arranged by size:
2246 1341 761 535
BclI T'GATC_A
Cuts at: 2403 2403
Size: 4883
BglI GCCn_nnn'nGGC
Cuts at: 1232 1232
Size: 4883
BglII A'GATC_T
Cuts at: 1262 2398 4435 1262
Size: 1136 2037 1710
Fragments arranged by size:
2037 1710 1136
BlpI GC'TnA_GC
Cuts at: 1995 1995
Size: 4883
Bme1580I G_kGCm'C
Cuts at: 730 2508 2601 3657 730
Size: 1778 93 1056 1956
Fragments arranged by size:
1956 1778 1056 93
BmgBI CAC'GTC
Cuts at: 1500 1500
Size: 4883
BmrI ACTGGGnnnn_n'
Cuts at: 1134 2095 2176 2511 4655 1134
Size: 961 81 335 2144 1362
Fragments arranged by size:
2144 1362 961 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3250 3250
Size: 4883
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 539 694 772 994 1432 1549 1692 1809
Size: 138 155 78 222 438 117 143 117
Cuts at: 1809 401
Size: 3475
Fragments arranged by size:
3475 438 222 155 143 138 117 117
78
Bpu10I CC'TnA_GC
Cuts at: 1127 1127
Size: 4883
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 2059 2938 3430 3728 1366
Size: 693 879 492 298 2521
Fragments arranged by size:
2521 879 693 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4587 4587
Size: 4883
BsaAI yAC'GTr
Cuts at: 2236 2236
Size: 4883
BsaBI GATnn'nnATC
Cuts at: 6 331 2397 4434 6
Size: 325 2066 2037 455
Fragments arranged by size:
2066 2037 455 325
BsaHI Gr'CG_yC
Cuts at: 2314 2562 3264 4760 2314
Size: 248 702 1496 2437
Fragments arranged by size:
2437 1496 702 248
BsaWI w'CCGG_w
Cuts at: 134 362 1277 2072 2593 3271 3545 3692 4103
Size: 228 915 795 521 678 274 147 411
Cuts at: 4103 134
Size: 914
Fragments arranged by size:
915 914 795 678 521 411 274 228
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 745 775 1547 1577 745
Size: 30 772 30 4051
Fragments arranged by size:
4051 772 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 622 1162 1219 1429 1842 1845 622
Size: 540 57 210 413 3 3660
Fragments arranged by size:
3660 540 413 210 57 3
BseYI C'CCAG_C
Cuts at: 583 1788 2308 3066 3643 4139 583
Size: 1205 520 758 577 496 1327
Fragments arranged by size:
1327 1205 758 577 520 496
BsiEI CG_ry'CG
Cuts at: 104 2425 2471 3679 104
Size: 2321 46 1208 1308
Fragments arranged by size:
2321 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1475 1897 2675 2865 3657 1475
Size: 422 778 190 792 2701
Fragments arranged by size:
2701 792 778 422 190
BsmI GAATG_Cn'
Cuts at: 1546 1546
Size: 4883
Bsp1286I G_dGCh'C
Cuts at: 319 458 730 1475 1745 1759 1897 2508 2601
Size: 139 272 745 270 14 138 611 93
Cuts at: 2601 2675 2865 2927 3657 319
Size: 74 190 62 730 1545
Fragments arranged by size:
1545 745 730 611 272 270 190 139
138 93 74 62 14
BspEI T'CCGG_A
Cuts at: 134 4103 134
Size: 3969 914
BspHI T'CATG_A
Cuts at: 1013 1475 4059 1013
Size: 462 2584 1837
Fragments arranged by size:
2584 1837 462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 578 1933 2147 2449 2830 578
Size: 1355 214 302 381 2631
Fragments arranged by size:
2631 1355 381 302 214
BsrBI CCG'CTC
Cuts at: 50 140 3175 3229 4713 4878 50
Size: 90 3035 54 1484 165 55
Fragments arranged by size:
3035 1484 165 90 55 54
BsrDI GCAATG_nn'
Cuts at: 1346 2795 1346
Size: 1449 3434
BsrFI r'CCGG_y
Cuts at: 362 409 1226 2881 3062 3271 362
Size: 47 817 1655 181 209 1974
Fragments arranged by size:
1974 1655 817 209 181 47
BssSI C'ACGA_G
Cuts at: 3154 3512 3154
Size: 358 4525
BstAPI GCAn_nnn'nTGC
Cuts at: 2260 2260
Size: 4883
BstBI TT'CG_AA
Cuts at: 3244 3244
Size: 4883
BstYI r'GATC_y
Cuts at: 1 1262 1908 2375 2398 2733 2979 3991 4077
Size: 1261 646 467 23 335 246 1012 86
Cuts at: 4077 4089 4435 1
Size: 12 346 449
Fragments arranged by size:
1261 1012 646 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 784 784
Size: 4883
BtgI C'CryG_G
Cuts at: 1085 1085
Size: 4883
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1107 1250 2833 3014 3177 1107
Size: 143 1583 181 163 2813
Fragments arranged by size:
2813 1583 181 163 143
BtsI GCAGTG_nn'
Cuts at: 905 1602 1623 1629 1698 1722 1782 1812 2126
Size: 697 21 6 69 24 60 30 314
Cuts at: 2126 905
Size: 3662
Fragments arranged by size:
3662 697 314 69 60 30 24 21
6
DraI TTT'AAA
Cuts at: 1882 1882
Size: 4883
DraIII CAC_nnn'GTG
Cuts at: 704 704
Size: 4883
DrdI GACnn_nn'nnGTC
Cuts at: 2589 3447 2589
Size: 858 4025
EaeI y'GGCC_r
Cuts at: 1224 1403 1779 2468 2642 3033 3060 4405 1224
Size: 179 376 689 174 391 27 1345 1702
Fragments arranged by size:
1702 1345 689 391 376 179 174 27
EagI C'GGCC_G
Cuts at: 2468 2468
Size: 4883
EarI CTCTTCn'nnn_
Cuts at: 1858 2906 3116 1858
Size: 1048 210 3625
Fragments arranged by size:
3625 1048 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 479 3091 3401 3547 4693 479
Size: 2612 310 146 1146 669
Fragments arranged by size:
2612 1146 669 310 146
EcoICRI GAG'CTC
Cuts at: 1473 1895 1473
Size: 422 4461
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 339 401 539 694 772 952 994 1432
Size: 198 62 138 155 78 180 42 438
Cuts at: 1432 1549 1692 1749 1809 2708 3140 3887 4155
Size: 117 143 57 60 899 432 747 268
Cuts at: 4155 141
Size: 869
Fragments arranged by size:
899 869 747 438 432 268 198 180
155 143 138 117 78 62 60 57
42
EcoNI CCTnn'n_nnAGG
Cuts at: 518 715 1426 518
Size: 197 711 3975
Fragments arranged by size:
3975 711 197
EcoO109I rG'GnC_Cy
Cuts at: 852 1419 2022 852
Size: 567 603 3713
Fragments arranged by size:
3713 603 567
EcoRI G'AATT_C
Cuts at: 1887 1887
Size: 4883
EcoRV GAT'ATC
Cuts at: 329 329
Size: 4883
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1041 2902 1041
Size: 1861 3022
FspI TGC'GCA
Cuts at: 2664 2664
Size: 4883
HaeII r_GCGC'y
Cuts at: 2317 2565 3587 4566 2317
Size: 248 1022 979 2634
Fragments arranged by size:
2634 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2725 2755 2757 2787 2725
Size: 30 2 30 4821
Fragments arranged by size:
4821 30 30 2
HincII GTy'rAC
Cuts at: 543 1575 1922 543
Size: 1032 347 3504
Fragments arranged by size:
3504 1032 347
KasI G'GCGC_C
Cuts at: 2313 2561 2313
Size: 248 4635
KpnI G_GTAC'C
Cuts at: 1903 1903
Size: 4883
MluI A'CGCG_T
Cuts at: 4316 4316
Size: 4883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1545 3554 3738 1545
Size: 2009 184 2690
Fragments arranged by size:
2690 2009 184
MscI TGG'CCA
Cuts at: 2644 2644
Size: 4883
MslI CAynn'nnrTG
Cuts at: 208 1018 1072 1235 2872 2999 208
Size: 810 54 163 1637 127 2092
Fragments arranged by size:
2092 1637 810 163 127 54
MspA1I CmG'CkG
Cuts at: 795 1222 1994 2308 2668 3681 3926 795
Size: 427 772 314 360 1013 245 1752
Fragments arranged by size:
1752 1013 772 427 360 314 245
NaeI GCC'GGC
Cuts at: 1228 3064 1228
Size: 1836 3047
NarI GG'CG_CC
Cuts at: 2314 2562 2314
Size: 248 4635
NcoI C'CATG_G
Cuts at: 1085 1085
Size: 4883
NgoMIV G'CCGG_C
Cuts at: 1226 3062 1226
Size: 1836 3047
NspI r_CATG'y
Cuts at: 849 1060 1936 2436 3343 849
Size: 211 876 500 907 2389
Fragments arranged by size:
2389 907 876 500 211
PciI A'CATG_T
Cuts at: 3339 3339
Size: 4883
PfoI T'CCnGG_A
Cuts at: 516 671 1858 516
Size: 155 1187 3541
Fragments arranged by size:
3541 1187 155
PmeI GTTT'AAAC
Cuts at: 1882 1882
Size: 4883
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 745 777 4022 4054 745
Size: 32 3245 32 1574
Fragments arranged by size:
3245 1574 32 32
PpuMI rG'GwC_Cy
Cuts at: 852 852
Size: 4883
PshAI GACnn'nnGTC
Cuts at: 1057 1057
Size: 4883
PstI C_TGCA'G
Cuts at: 719 1214 1340 1427 1930 2615 719
Size: 495 126 87 503 685 2987
Fragments arranged by size:
2987 685 503 495 126 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4883
PvuII CAG'CTG
Cuts at: 795 1222 2308 2668 795
Size: 427 1086 360 3010
Fragments arranged by size:
3010 1086 427 360
RsrII CG'GwC_CG
Cuts at: 218 3078 218
Size: 2860 2023
SacI G_AGCT'C
Cuts at: 1475 1897 1475
Size: 422 4461
SalI G'TCGA_C
Cuts at: 1920 1920
Size: 4883
SapI GCTCTTCn'nnn_
Cuts at: 1858 2906 3116 1858
Size: 1048 210 3625
Fragments arranged by size:
3625 1048 210
SbfI CC_TGCA'GG
Cuts at: 1930 1930
Size: 4883
ScaI AGT'ACT
Cuts at: 4400 4400
Size: 4883
SexAI A'CCwGG_T
Cuts at: 854 854
Size: 4883
SfcI C'TryA_G
Cuts at: 33 715 1210 1336 1423 1926 2611 3604 3795
Size: 682 495 126 87 503 685 993 191
Cuts at: 3795 33
Size: 1121
Fragments arranged by size:
1121 993 685 682 503 495 191 126
87
SfoI GGC'GCC
Cuts at: 2315 2563 2315
Size: 248 4635
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4883
SmaI CCC'GGG
Cuts at: 1905 1905
Size: 4883
SmlI C'TyrA_G
Cuts at: 1381 2038 2953 3445 3707 1381
Size: 657 915 492 262 2557
Fragments arranged by size:
2557 915 657 492 262
SpeI A'CTAG_T
Cuts at: 67 340 67
Size: 273 4610
SphI G_CATG'C
Cuts at: 1936 2436 1936
Size: 500 4383
StyI C'CwwG_G
Cuts at: 980 1085 1731 2017 2371 980
Size: 105 646 286 354 3492
Fragments arranged by size:
3492 646 354 286 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 268 619 2466 2599 3058 3267 268
Size: 351 1847 133 459 209 1884
Fragments arranged by size:
1884 1847 459 351 209 133
TatI w'GTAC_w
Cuts at: 770 4398 770
Size: 3628 1255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 688 720 4400 4432 688
Size: 32 3680 32 1139
Fragments arranged by size:
3680 1139 32 32
Tth111I GACn'n_nGTC
Cuts at: 1312 2680 1312
Size: 1368 3515
XbaI T'CTAG_A
Cuts at: 1914 1914
Size: 4883
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1398 1440 1491 2378 1398
Size: 42 51 887 3903
Fragments arranged by size:
3903 887 51 42
XmaI C'CCGG_G
Cuts at: 1903 1903
Size: 4883
XmnI GAAnn'nnTTC
Cuts at: 1021 1867 1021
Size: 846 4037
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
BcgI BfrBI BmtI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI