Restriction map for
pF1KB6128
(Circular) (Six-base) MAPSORT of: pF1KB6128.seq Check: 5225 from: 1 to: 4994
pF1KB6128 4994 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 457 457
Size: 4994
AccI GT'mk_AC
Cuts at: 2032 2032
Size: 4994
Acc65I G'GTAC_C
Cuts at: 2010 2010
Size: 4994
AclI AA'CG_TT
Cuts at: 4834 4834
Size: 4994
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 1520 1654 2819 3251 3998 4266 1282
Size: 238 134 1165 432 747 268 2010
Fragments arranged by size:
2010 1165 747 432 268 238 134
AflIII A'CryG_T
Cuts at: 2346 3450 4427 2346
Size: 1104 977 2913
Fragments arranged by size:
2913 1104 977
AgeI A'CCGG_T
Cuts at: 3382 3382
Size: 4994
AleI CACnn'nnGTG
Cuts at: 1039 1481 1039
Size: 442 4552
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1864 1864
Size: 4994
AlwNI CAG_nnn'CTG
Cuts at: 849 1207 1254 2299 3866 849
Size: 358 47 1045 1567 1977
Fragments arranged by size:
1977 1567 1045 358 47
ApaI G_GGCC'C
Cuts at: 903 903
Size: 4994
ApaLI G'TGCA_C
Cuts at: 3764 3764
Size: 4994
ApoI r'AATT_y
Cuts at: 506 1998 4347 4603 506
Size: 1492 2349 256 897
Fragments arranged by size:
2349 1492 897 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4994
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4994
AvaI C'yCGr_G
Cuts at: 914 1108 1867 2014 914
Size: 194 759 147 3894
Fragments arranged by size:
3894 759 194 147
AvrII C'CTAG_G
Cuts at: 1968 1968
Size: 4994
BamHI G'GATC_C
Cuts at: 2019 2019
Size: 4994
BanI G'GyrC_C
Cuts at: 693 1840 2010 2424 2672 2707 693
Size: 1147 170 414 248 35 2980
Fragments arranged by size:
2980 1147 414 248 170 35
BanII G_rGCy'C
Cuts at: 259 580 837 903 979 1022 1109 1944 2008
Size: 321 257 66 76 43 87 835 64
Cuts at: 2008 3038 259
Size: 1030 2215
Fragments arranged by size:
2215 1030 835 321 257 87 76 66
64 43
BbeI G_GCGC'C
Cuts at: 2428 2676 2428
Size: 248 4746
BbsI GAAGACnn'nnnn_
Cuts at: 1180 1906 1180
Size: 726 4268
BbvCI CC'TCA_GC
Cuts at: 265 698 1906 265
Size: 433 1208 3353
Fragments arranged by size:
3353 1208 433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 597 631 651 685 718 752 1279 1313 597
Size: 34 20 34 33 34 527 34 4278
Fragments arranged by size:
4278 527 34 34 34 34 33 20
BciVI GTATCCnnnnn_n'
Cuts at: 2892 3653 4994 2892
Size: 761 1341 2892
Fragments arranged by size:
2892 1341 761
BclI T'GATC_A
Cuts at: 239 2514 239
Size: 2275 2719
BglI GCCn_nnn'nGGC
Cuts at: 152 157 1970 152
Size: 5 1813 3176
Fragments arranged by size:
3176 1813 5
BglII A'GATC_T
Cuts at: 1646 2509 4546 1646
Size: 863 2037 2094
Fragments arranged by size:
2094 2037 863
BlpI GC'TnA_GC
Cuts at: 422 836 2106 422
Size: 414 1270 3310
Fragments arranged by size:
3310 1270 414
Bme1580I G_kGCm'C
Cuts at: 903 1341 2619 2712 3768 903
Size: 438 1278 93 1056 2129
Fragments arranged by size:
2129 1278 1056 438 93
BmrI ACTGGGnnnn_n'
Cuts at: 377 798 2206 2287 2622 4766 377
Size: 421 1408 81 335 2144 605
Fragments arranged by size:
2144 1408 605 421 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 338 3361 338
Size: 3023 1971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 556 571 1036 1570 1621 1936 152
Size: 404 15 465 534 51 315 3210
Fragments arranged by size:
3210 534 465 404 315 51 15
Bpu10I CC'TnA_GC
Cuts at: 265 323 698 1094 1906 265
Size: 58 375 396 812 3353
Fragments arranged by size:
3353 812 396 375 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2170 3049 3541 3839 2170
Size: 879 492 298 3325
Fragments arranged by size:
3325 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 395 1856 4698 395
Size: 1461 2842 691
Fragments arranged by size:
2842 1461 691
BsaAI yAC'GTr
Cuts at: 1085 2347 1085
Size: 1262 3732
BsaBI GATnn'nnATC
Cuts at: 6 2508 4545 6
Size: 2502 2037 455
Fragments arranged by size:
2502 2037 455
BsaHI Gr'CG_yC
Cuts at: 1184 2425 2673 3375 4871 1184
Size: 1241 248 702 1496 1307
Fragments arranged by size:
1496 1307 1241 702 248
BsaWI w'CCGG_w
Cuts at: 797 1619 1706 2183 2704 3382 3656 3803 4214
Size: 822 87 477 521 678 274 147 411
Cuts at: 4214 797
Size: 1577
Fragments arranged by size:
1577 822 678 521 477 411 274 147
87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 951 981 1096 1126 951
Size: 30 115 30 4819
Fragments arranged by size:
4819 115 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 475 715 1168 1381 1534 1885 127
Size: 348 240 453 213 153 351 3236
Fragments arranged by size:
3236 453 351 348 240 213 153
BseYI C'CCAG_C
Cuts at: 884 949 1340 1589 1890 2419 3177 3754 4250
Size: 65 391 249 301 529 758 577 496
Cuts at: 4250 884
Size: 1628
Fragments arranged by size:
1628 758 577 529 496 391 301 249
65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1216 1459 1780 196
Size: 1020 243 321 3410
Fragments arranged by size:
3410 1020 321 243
BsiEI CG_ry'CG
Cuts at: 104 2536 2582 3790 104
Size: 2432 46 1208 1308
Fragments arranged by size:
2432 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 969 1109 2008 2786 2976 3768 969
Size: 140 899 778 190 792 2195
Fragments arranged by size:
2195 899 792 778 190 140
Bsp1286I G_dGCh'C
Cuts at: 259 580 837 903 969 979 1022 1109 1341
Size: 321 257 66 66 10 43 87 232
Cuts at: 1341 1944 2008 2619 2712 2786 2976 3038 3768
Size: 603 64 611 93 74 190 62 730
Cuts at: 3768 259
Size: 1485
Fragments arranged by size:
1485 730 611 603 321 257 232 190
93 87 74 66 66 64 62 43
10
BspEI T'CCGG_A
Cuts at: 1619 4214 1619
Size: 2595 2399
BspHI T'CATG_A
Cuts at: 4170 4170
Size: 4994
BspMI ACCTGCnnnn'nnnn_
Cuts at: 413 457 2044 2258 2560 2941 413
Size: 44 1587 214 302 381 2466
Fragments arranged by size:
2466 1587 381 302 214 44
BsrBI CCG'CTC
Cuts at: 50 3286 3340 4824 4989 50
Size: 3236 54 1484 165 55
Fragments arranged by size:
3236 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2906 2906
Size: 4994
BsrFI r'CCGG_y
Cuts at: 151 770 1214 2992 3173 3382 151
Size: 619 444 1778 181 209 1763
Fragments arranged by size:
1778 1763 619 444 209 181
BssHII G'CGCG_C
Cuts at: 186 186
Size: 4994
BssSI C'ACGA_G
Cuts at: 1798 3265 3623 1798
Size: 1467 358 3169
Fragments arranged by size:
3169 1467 358
BstAPI GCAn_nnn'nTGC
Cuts at: 183 849 1254 2371 183
Size: 666 405 1117 2806
Fragments arranged by size:
2806 1117 666 405
BstBI TT'CG_AA
Cuts at: 3355 3355
Size: 4994
BstXI CCAn_nnnn'nTGG
Cuts at: 204 318 612 204
Size: 114 294 4586
Fragments arranged by size:
4586 294 114
BstYI r'GATC_y
Cuts at: 1 1463 1646 1922 2019 2486 2509 2844 3090
Size: 1462 183 276 97 467 23 335 246
Cuts at: 3090 4102 4188 4200 4546 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1462 1012 467 449 346 335 276 246
183 97 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 971 971
Size: 4994
BtgI C'CryG_G
Cuts at: 107 646 107
Size: 539 4455
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2944 3125 3288 2944
Size: 181 163 4650
Fragments arranged by size:
4650 181 163
BtsI GCAGTG_nn'
Cuts at: 523 2237 523
Size: 1714 3280
DraI TTT'AAA
Cuts at: 1993 1993
Size: 4994
DrdI GACnn_nn'nnGTC
Cuts at: 2700 3558 2700
Size: 858 4136
EaeI y'GGCC_r
Cuts at: 308 772 2579 2753 3144 3171 4516 308
Size: 464 1807 174 391 27 1345 786
Fragments arranged by size:
1807 1345 786 464 391 174 27
EagI C'GGCC_G
Cuts at: 2579 2579
Size: 4994
EarI CTCTTCn'nnn_
Cuts at: 418 1258 1496 1628 3017 3227 418
Size: 840 238 132 1389 210 2185
Fragments arranged by size:
2185 1389 840 238 210 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 133 292 1936 3202 3512 3658 4804 133
Size: 159 1644 1266 310 146 1146 323
Fragments arranged by size:
1644 1266 1146 323 310 159 146
EcoICRI GAG'CTC
Cuts at: 1107 2006 1107
Size: 899 4095
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 556 571 1036 1282 1520 1570 1621 1654
Size: 404 15 465 246 238 50 51 33
Cuts at: 1654 1936 2819 3251 3998 4266 152
Size: 282 883 432 747 268 880
Fragments arranged by size:
883 880 747 465 432 404 282 268
246 238 51 50 33 15
EcoNI CCTnn'n_nnAGG
Cuts at: 322 1948 322
Size: 1626 3368
EcoO109I rG'GnC_Cy
Cuts at: 534 888 899 900 918 1251 1383 2133 534
Size: 354 11 1 18 333 132 750 3395
Fragments arranged by size:
3395 750 354 333 132 18 11 1
EcoRI G'AATT_C
Cuts at: 1998 1998
Size: 4994
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1023 3013 1023
Size: 1990 3004
FspI TGC'GCA
Cuts at: 2775 2775
Size: 4994
HaeII r_GCGC'y
Cuts at: 2428 2676 3698 4677 2428
Size: 248 1022 979 2745
Fragments arranged by size:
2745 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 855 887 951 983 1096 1128 1426 1458 2836
Size: 32 64 32 113 32 298 32 1378
Cuts at: 2836 2866 2868 2898 855
Size: 30 2 30 2951
Fragments arranged by size:
2951 1378 298 113 64 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2033 2033
Size: 4994
KasI G'GCGC_C
Cuts at: 2424 2672 2424
Size: 248 4746
KpnI G_GTAC'C
Cuts at: 2014 2014
Size: 4994
MluI A'CGCG_T
Cuts at: 4427 4427
Size: 4994
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 687 3665 3849 687
Size: 2978 184 1832
Fragments arranged by size:
2978 1832 184
MscI TGG'CCA
Cuts at: 310 2755 310
Size: 2445 2549
MslI CAynn'nnrTG
Cuts at: 1039 1481 1846 2983 3110 1039
Size: 442 365 1137 127 2923
Fragments arranged by size:
2923 1137 442 365 127
MspA1I CmG'CkG
Cuts at: 227 519 840 846 1128 1344 1536 2105 2419
Size: 292 321 6 282 216 192 569 314
Cuts at: 2419 2779 3792 4037 227
Size: 360 1013 245 1184
Fragments arranged by size:
1184 1013 569 360 321 314 292 282
245 216 192 6
NaeI GCC'GGC
Cuts at: 153 1216 3175 153
Size: 1063 1959 1972
Fragments arranged by size:
1972 1959 1063
NarI GG'CG_CC
Cuts at: 2425 2673 2425
Size: 248 4746
NcoI C'CATG_G
Cuts at: 107 107
Size: 4994
NgoMIV G'CCGG_C
Cuts at: 151 1214 3173 151
Size: 1063 1959 1972
Fragments arranged by size:
1972 1959 1063
NspI r_CATG'y
Cuts at: 2047 2547 3454 2047
Size: 500 907 3587
Fragments arranged by size:
3587 907 500
PasI CC'CwG_GG
Cuts at: 1179 1179
Size: 4994
PciI A'CATG_T
Cuts at: 3450 3450
Size: 4994
PfoI T'CCnGG_A
Cuts at: 1598 1925 1979 1598
Size: 327 54 4613
Fragments arranged by size:
4613 327 54
PmeI GTTT'AAAC
Cuts at: 1993 1993
Size: 4994
PmlI CAC'GTG
Cuts at: 1085 1085
Size: 4994
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1091 1123 1720 1752 4133 4165 1091
Size: 32 597 32 2381 32 1920
Fragments arranged by size:
2381 1920 597 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 534 918 1383 534
Size: 384 465 4145
Fragments arranged by size:
4145 465 384
PspOMI G'GGCC_C
Cuts at: 899 899
Size: 4994
PspXI vC'TCGA_Gb
Cuts at: 1108 1867 1108
Size: 759 4235
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 361 393 361
Size: 32 4962
PstI C_TGCA'G
Cuts at: 468 566 746 854 938 1319 1424 1810 2041
Size: 98 180 108 84 381 105 386 231
Cuts at: 2041 2726 468
Size: 685 2736
Fragments arranged by size:
2736 685 386 381 231 180 108 105
98 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4994
PvuII CAG'CTG
Cuts at: 519 1128 1536 2419 2779 519
Size: 609 408 883 360 2734
Fragments arranged by size:
2734 883 609 408 360
RsrII CG'GwC_CG
Cuts at: 120 3189 120
Size: 3069 1925
SacI G_AGCT'C
Cuts at: 1109 2008 1109
Size: 899 4095
SalI G'TCGA_C
Cuts at: 2031 2031
Size: 4994
SanDI GG'GwC_CC
Cuts at: 534 1383 534
Size: 849 4145
SapI GCTCTTCn'nnn_
Cuts at: 1258 3017 3227 1258
Size: 1759 210 3025
Fragments arranged by size:
3025 1759 210
SbfI CC_TGCA'GG
Cuts at: 468 2041 468
Size: 1573 3421
ScaI AGT'ACT
Cuts at: 4511 4511
Size: 4994
SfcI C'TryA_G
Cuts at: 33 464 562 742 850 934 1315 1420 1806
Size: 431 98 180 108 84 381 105 386
Cuts at: 1806 2037 2722 3715 3906 33
Size: 231 685 993 191 1121
Fragments arranged by size:
1121 993 685 431 386 381 231 191
180 108 105 98 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 1970 1970
Size: 4994
SfoI GGC'GCC
Cuts at: 2426 2674 2426
Size: 248 4746
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4994
SmaI CCC'GGG
Cuts at: 916 2016 916
Size: 1100 3894
SmlI C'TyrA_G
Cuts at: 1108 1867 2149 3064 3556 3818 1108
Size: 759 282 915 492 262 2284
Fragments arranged by size:
2284 915 759 492 282 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4994
SphI G_CATG'C
Cuts at: 2047 2547 2047
Size: 500 4494
StuI AGG'CCT
Cuts at: 328 940 1774 328
Size: 612 834 3548
Fragments arranged by size:
3548 834 612
StyI C'CwwG_G
Cuts at: 107 350 529 1968 2128 2482 107
Size: 243 179 1439 160 354 2619
Fragments arranged by size:
2619 1439 354 243 179 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 137 1603 2577 2710 3169 3378 137
Size: 1466 974 133 459 209 1753
Fragments arranged by size:
1753 1466 974 459 209 133
TatI w'GTAC_w
Cuts at: 1231 4509 1231
Size: 3278 1716
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 147 179 638 670 787 819 4511 4543 147
Size: 32 459 32 117 32 3692 32 598
Fragments arranged by size:
3692 598 459 117 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 790 2791 790
Size: 2001 2993
XbaI T'CTAG_A
Cuts at: 2025 2025
Size: 4994
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1957 2489 1957
Size: 532 4462
XhoI C'TCGA_G
Cuts at: 1108 1867 1108
Size: 759 4235
XmaI C'CCGG_G
Cuts at: 914 2014 914
Size: 1100 3894
XmnI GAAnn'nnTTC
Cuts at: 595 595
Size: 4994
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI BaeI BfrBI
BmgBI BmtI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PshAI PsiI SacII SexAI SgrAI SnaBI SrfI SspI
SwaI ZraI