Restriction map for
pF1KB6127
(Circular) (Six-base) MAPSORT of: pF1KB6127.seq Check: 8686 from: 1 to: 4874
pF1KB6127 4874 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AatII G_ACGT'C
Cuts at: 1286 1286
Size: 4874
AccI GT'mk_AC
Cuts at: 1912 1912
Size: 4874
Acc65I G'GTAC_C
Cuts at: 1890 1890
Size: 4874
AclI AA'CG_TT
Cuts at: 4714 4714
Size: 4874
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1702 2699 3131 3878 4146 1702
Size: 997 432 747 268 2430
Fragments arranged by size:
2430 997 747 432 268
AflIII A'CryG_T
Cuts at: 875 1232 2226 3330 4307 875
Size: 357 994 1104 977 1442
Fragments arranged by size:
1442 1104 994 977 357
AgeI A'CCGG_T
Cuts at: 3262 3262
Size: 4874
AhdI GACnn_n'nnGTC
Cuts at: 876 876
Size: 4874
AlwNI CAG_nnn'CTG
Cuts at: 732 1029 1341 2179 3746 732
Size: 297 312 838 1567 1860
Fragments arranged by size:
1860 1567 838 312 297
ApaI G_GGCC'C
Cuts at: 207 1628 207
Size: 1421 3453
ApaLI G'TGCA_C
Cuts at: 3644 3644
Size: 4874
ApoI r'AATT_y
Cuts at: 1878 4227 4483 1878
Size: 2349 256 2269
Fragments arranged by size:
2349 2269 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4874
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4874
AvaI C'yCGr_G
Cuts at: 200 575 1165 1894 200
Size: 375 590 729 3180
Fragments arranged by size:
3180 729 590 375
BamHI G'GATC_C
Cuts at: 1899 1899
Size: 4874
BanI G'GyrC_C
Cuts at: 273 943 1161 1264 1890 2304 2552 2587 273
Size: 670 218 103 626 414 248 35 2560
Fragments arranged by size:
2560 670 626 414 248 218 103 35
BanII G_rGCy'C
Cuts at: 207 365 1475 1568 1628 1888 2918 207
Size: 158 1110 93 60 260 1030 2163
Fragments arranged by size:
2163 1110 1030 260 158 93 60
BbeI G_GCGC'C
Cuts at: 277 1165 1268 2308 2556 277
Size: 888 103 1040 248 2595
Fragments arranged by size:
2595 1040 888 248 103
BbsI GAAGACnn'nnnn_
Cuts at: 334 1193 334
Size: 859 4015
BciVI GTATCCnnnnn_n'
Cuts at: 2772 3533 4874 2772
Size: 761 1341 2772
Fragments arranged by size:
2772 1341 761
BclI T'GATC_A
Cuts at: 335 2394 335
Size: 2059 2815
BglI GCCn_nnn'nGGC
Cuts at: 416 1067 1493 416
Size: 651 426 3797
Fragments arranged by size:
3797 651 426
BglII A'GATC_T
Cuts at: 2389 4426 2389
Size: 2037 2837
BlpI GC'TnA_GC
Cuts at: 1129 1986 1129
Size: 857 4017
Bme1580I G_kGCm'C
Cuts at: 207 1628 2499 2592 3648 207
Size: 1421 871 93 1056 1433
Fragments arranged by size:
1433 1421 1056 871 93
BmgBI CAC'GTC
Cuts at: 1146 1146
Size: 4874
BmrI ACTGGGnnnn_n'
Cuts at: 1719 2086 2167 2502 4646 1719
Size: 367 81 335 2144 1947
Fragments arranged by size:
2144 1947 367 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3241 3241
Size: 4874
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 736 1138 1512 150
Size: 586 402 374 3512
Fragments arranged by size:
3512 586 402 374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 843 1423 2050 2929 3421 3719 843
Size: 580 627 879 492 298 1998
Fragments arranged by size:
1998 879 627 580 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4578 4578
Size: 4874
BsaAI yAC'GTr
Cuts at: 1653 2227 1653
Size: 574 4300
BsaBI GATnn'nnATC
Cuts at: 6 2388 4425 6
Size: 2382 2037 455
Fragments arranged by size:
2382 2037 455
BsaHI Gr'CG_yC
Cuts at: 274 950 1070 1162 1265 1283 1367 2305 2553
Size: 676 120 92 103 18 84 938 248
Cuts at: 2553 3255 4751 274
Size: 702 1496 397
Fragments arranged by size:
1496 938 702 676 397 248 120 103
92 84 18
BsaWI w'CCGG_w
Cuts at: 2063 2584 3262 3536 3683 4094 2063
Size: 521 678 274 147 411 2843
Fragments arranged by size:
2843 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 165 195 737 767 979 1009 165
Size: 30 542 30 212 30 4030
Fragments arranged by size:
4030 542 212 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 758 928 758
Size: 170 4704
BseYI C'CCAG_C
Cuts at: 266 1567 1616 2299 3057 3634 4130 266
Size: 1301 49 683 758 577 496 1010
Fragments arranged by size:
1301 1010 758 683 577 496 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 161 373 525 658 673 970 1042 1066
Size: 18 212 152 133 15 297 72 24
Cuts at: 1066 1597 1684 143
Size: 531 87 3333
Fragments arranged by size:
3333 531 297 212 152 133 87 72
24 18 15
BsiEI CG_ry'CG
Cuts at: 104 1094 1556 2416 2462 3670 104
Size: 990 462 860 46 1208 1308
Fragments arranged by size:
1308 1208 990 860 462 46
BsiHKAI G_wGCw'C
Cuts at: 1888 2666 2856 3648 1888
Size: 778 190 792 3114
Fragments arranged by size:
3114 792 778 190
BsmI GAATG_Cn'
Cuts at: 771 771
Size: 4874
Bsp1286I G_dGCh'C
Cuts at: 207 365 1475 1568 1628 1888 2499 2592 2666
Size: 158 1110 93 60 260 611 93 74
Cuts at: 2666 2856 2918 3648 207
Size: 190 62 730 1433
Fragments arranged by size:
1433 1110 730 611 260 190 158 93
93 74 62 60
BspEI T'CCGG_A
Cuts at: 4094 4094
Size: 4874
BspHI T'CATG_A
Cuts at: 4050 4050
Size: 4874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1523 1924 2138 2440 2821 1523
Size: 401 214 302 381 3576
Fragments arranged by size:
3576 401 381 302 214
BsrBI CCG'CTC
Cuts at: 50 588 685 740 1824 3166 3220 4704 4869
Size: 538 97 55 1084 1342 54 1484 165
Cuts at: 4869 50
Size: 55
Fragments arranged by size:
1484 1342 1084 538 165 97 55 55
54
BsrDI GCAATG_nn'
Cuts at: 714 2786 714
Size: 2072 2802
BsrFI r'CCGG_y
Cuts at: 195 1035 1190 1512 2872 3053 3262 195
Size: 840 155 322 1360 181 209 1807
Fragments arranged by size:
1807 1360 840 322 209 181 155
BssHII G'CGCG_C
Cuts at: 1133 1135 1170 1172 1174 1133
Size: 2 35 2 2 4833
Fragments arranged by size:
4833 35 2 2 2
BssSI C'ACGA_G
Cuts at: 391 691 1300 3145 3503 391
Size: 300 609 1845 358 1762
Fragments arranged by size:
1845 1762 609 358 300
BstAPI GCAn_nnn'nTGC
Cuts at: 1029 1341 2251 1029
Size: 312 910 3652
Fragments arranged by size:
3652 910 312
BstBI TT'CG_AA
Cuts at: 1776 3235 1776
Size: 1459 3415
BstEII G'GTnAC_C
Cuts at: 1038 1038
Size: 4874
BstYI r'GATC_y
Cuts at: 1 728 1899 2366 2389 2724 2970 3982 4068
Size: 727 1171 467 23 335 246 1012 86
Cuts at: 4068 4080 4426 1
Size: 12 346 449
Fragments arranged by size:
1171 1012 727 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 672 1314 1350 1550 672
Size: 642 36 200 3996
Fragments arranged by size:
3996 642 200 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 326 975 2824 3005 3168 326
Size: 649 1849 181 163 2032
Fragments arranged by size:
2032 1849 649 181 163
BtsI GCAGTG_nn'
Cuts at: 173 532 2117 173
Size: 359 1585 2930
Fragments arranged by size:
2930 1585 359
ClaI AT'CG_AT
Cuts at: 1634 1634
Size: 4874
DraI TTT'AAA
Cuts at: 1873 1873
Size: 4874
DraIII CAC_nnn'GTG
Cuts at: 943 943
Size: 4874
DrdI GACnn_nn'nnGTC
Cuts at: 718 1714 2580 3438 718
Size: 996 866 858 2154
Fragments arranged by size:
2154 996 866 858
EaeI y'GGCC_r
Cuts at: 408 794 1091 1304 1553 2459 2633 3024 3051
Size: 386 297 213 249 906 174 391 27
Cuts at: 3051 4396 408
Size: 1345 886
Fragments arranged by size:
1345 906 886 391 386 297 249 213
174 27
EagI C'GGCC_G
Cuts at: 1091 1553 2459 1091
Size: 462 906 3506
Fragments arranged by size:
3506 906 462
EarI CTCTTCn'nnn_
Cuts at: 311 2897 3107 311
Size: 2586 210 2078
Fragments arranged by size:
2586 2078 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1300 3082 3392 3538 4684 1300
Size: 1782 310 146 1146 1490
Fragments arranged by size:
1782 1490 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1886 1886
Size: 4874
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 736 1138 1512 1702 2699 3131 3878 4146
Size: 586 402 374 190 997 432 747 268
Cuts at: 4146 150
Size: 878
Fragments arranged by size:
997 878 747 586 432 402 374 268
190
EcoNI CCTnn'n_nnAGG
Cuts at: 475 889 1530 475
Size: 414 641 3819
Fragments arranged by size:
3819 641 414
EcoO109I rG'GnC_Cy
Cuts at: 204 1624 2013 204
Size: 1420 389 3065
Fragments arranged by size:
3065 1420 389
EcoRI G'AATT_C
Cuts at: 1878 1878
Size: 4874
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 990 2893 990
Size: 1903 2971
FspI TGC'GCA
Cuts at: 1327 2655 1327
Size: 1328 3546
HaeII r_GCGC'y
Cuts at: 277 1126 1165 1268 2308 2556 3578 4557 277
Size: 849 39 103 1040 248 1022 979 594
Fragments arranged by size:
1040 1022 979 849 594 248 103 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 685 717 862 894 1579 1611 2716 2746 2748
Size: 32 145 32 685 32 1105 30 2
Cuts at: 2748 2778 685
Size: 30 2781
Fragments arranged by size:
2781 1105 685 145 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1224 1913 1224
Size: 689 4185
KasI G'GCGC_C
Cuts at: 273 1161 1264 2304 2552 273
Size: 888 103 1040 248 2595
Fragments arranged by size:
2595 1040 888 248 103
KpnI G_GTAC'C
Cuts at: 1894 1894
Size: 4874
MluI A'CGCG_T
Cuts at: 875 4307 875
Size: 3432 1442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 590 794 3545 3729 590
Size: 204 2751 184 1735
Fragments arranged by size:
2751 1735 204 184
MscI TGG'CCA
Cuts at: 410 1306 2635 410
Size: 896 1329 2649
Fragments arranged by size:
2649 1329 896
MslI CAynn'nnrTG
Cuts at: 1408 2863 2990 1408
Size: 1455 127 3292
Fragments arranged by size:
3292 1455 127
MspA1I CmG'CkG
Cuts at: 270 387 665 674 984 1080 1158 1338 1552
Size: 117 278 9 310 96 78 180 214
Cuts at: 1552 1616 1985 2299 2659 3672 3917 270
Size: 64 369 314 360 1013 245 1227
Fragments arranged by size:
1227 1013 369 360 314 310 278 245
214 180 117 96 78 64 9
NaeI GCC'GGC
Cuts at: 197 1514 3055 197
Size: 1317 1541 2016
Fragments arranged by size:
2016 1541 1317
NarI GG'CG_CC
Cuts at: 274 1162 1265 2305 2553 274
Size: 888 103 1040 248 2595
Fragments arranged by size:
2595 1040 888 248 103
NgoMIV G'CCGG_C
Cuts at: 195 1512 3053 195
Size: 1317 1541 2016
Fragments arranged by size:
2016 1541 1317
NspI r_CATG'y
Cuts at: 1927 2427 3334 1927
Size: 500 907 3467
Fragments arranged by size:
3467 907 500
PasI CC'CwG_GG
Cuts at: 448 448
Size: 4874
PciI A'CATG_T
Cuts at: 3330 3330
Size: 4874
PfoI T'CCnGG_A
Cuts at: 1643 1643
Size: 4874
PmeI GTTT'AAAC
Cuts at: 1873 1873
Size: 4874
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 4842
PspOMI G'GGCC_C
Cuts at: 203 1624 203
Size: 1421 3453
PstI C_TGCA'G
Cuts at: 437 476 1921 2606 437
Size: 39 1445 685 2705
Fragments arranged by size:
2705 1445 685 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4874
PvuII CAG'CTG
Cuts at: 270 1338 2299 2659 270
Size: 1068 961 360 2485
Fragments arranged by size:
2485 1068 961 360
RsrII CG'GwC_CG
Cuts at: 3069 3069
Size: 4874
SacI G_AGCT'C
Cuts at: 1888 1888
Size: 4874
SacII CC_GC'GG
Cuts at: 675 1553 675
Size: 878 3996
SalI G'TCGA_C
Cuts at: 1911 1911
Size: 4874
SapI GCTCTTCn'nnn_
Cuts at: 2897 3107 2897
Size: 210 4664
SbfI CC_TGCA'GG
Cuts at: 476 1921 476
Size: 1445 3429
ScaI AGT'ACT
Cuts at: 133 4391 133
Size: 4258 616
SfcI C'TryA_G
Cuts at: 33 433 472 1011 1917 2602 3595 3786 33
Size: 400 39 539 906 685 993 191 1121
Fragments arranged by size:
1121 993 906 685 539 400 191 39
SfoI GGC'GCC
Cuts at: 275 1163 1266 2306 2554 275
Size: 888 103 1040 248 2595
Fragments arranged by size:
2595 1040 888 248 103
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4874
SmaI CCC'GGG
Cuts at: 202 1896 202
Size: 1694 3180
SmlI C'TyrA_G
Cuts at: 822 1438 2029 2944 3436 3698 822
Size: 616 591 915 492 262 1998
Fragments arranged by size:
1998 915 616 591 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4874
SphI G_CATG'C
Cuts at: 1927 2427 1927
Size: 500 4374
SrfI GCCC'GGGC
Cuts at: 202 202
Size: 4874
StuI AGG'CCT
Cuts at: 322 820 895 322
Size: 498 75 4301
Fragments arranged by size:
4301 498 75
StyI C'CwwG_G
Cuts at: 222 749 2008 2362 222
Size: 527 1259 354 2734
Fragments arranged by size:
2734 1259 527 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1879 2457 2590 3049 3258 1879
Size: 578 133 459 209 3495
Fragments arranged by size:
3495 578 459 209 133
TatI w'GTAC_w
Cuts at: 131 1208 4389 131
Size: 1077 3181 616
Fragments arranged by size:
3181 1077 616
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 165 197 737 769 979 1011 4391 4423 165
Size: 32 540 32 210 32 3380 32 616
Fragments arranged by size:
3380 616 540 210 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2671 2671
Size: 4874
XbaI T'CTAG_A
Cuts at: 1905 1905
Size: 4874
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2369 2369
Size: 4874
XmaI C'CCGG_G
Cuts at: 200 1894 200
Size: 1694 3180
ZraI GAC'GTC
Cuts at: 1284 1284
Size: 4874
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI
BsrGI BstXI BstZ17I Bsu36I CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PpuMI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SspI SwaI
XhoI XmnI