Restriction map for
pF1KB6125
(Circular) (Six-base) MAPSORT of: pF1KB6125.seq Check: 7684 from: 1 to: 4871
pF1KB6125 4871 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 285 285
Size: 4871
AccI GT'mk_AC
Cuts at: 1909 1909
Size: 4871
Acc65I G'GTAC_C
Cuts at: 1887 1887
Size: 4871
AclI AA'CG_TT
Cuts at: 4711 4711
Size: 4871
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 1597 1781 2696 3128 3875 4143 625
Size: 972 184 915 432 747 268 1353
Fragments arranged by size:
1353 972 915 747 432 268 184
AflIII A'CryG_T
Cuts at: 2223 3327 4304 2223
Size: 1104 977 2790
Fragments arranged by size:
2790 1104 977
AgeI A'CCGG_T
Cuts at: 3259 3259
Size: 4871
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 884 884
Size: 4871
AlwNI CAG_nnn'CTG
Cuts at: 1423 2176 3743 1423
Size: 753 1567 2551
Fragments arranged by size:
2551 1567 753
ApaLI G'TGCA_C
Cuts at: 3641 3641
Size: 4871
ApoI r'AATT_y
Cuts at: 534 1462 1704 1875 4224 4480 534
Size: 928 242 171 2349 256 925
Fragments arranged by size:
2349 928 925 256 242 171
AseI AT'TA_AT
Cuts at: 20 20
Size: 4871
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4871
AvaI C'yCGr_G
Cuts at: 1310 1891 1310
Size: 581 4290
BamHI G'GATC_C
Cuts at: 1896 1896
Size: 4871
BanI G'GyrC_C
Cuts at: 496 1887 2301 2549 2584 496
Size: 1391 414 248 35 2783
Fragments arranged by size:
2783 1391 414 248 35
BanII G_rGCy'C
Cuts at: 178 224 332 1885 2915 178
Size: 46 108 1553 1030 2134
Fragments arranged by size:
2134 1553 1030 108 46
BbeI G_GCGC'C
Cuts at: 500 2305 2553 500
Size: 1805 248 2818
Fragments arranged by size:
2818 1805 248
BbvCI CC'TCA_GC
Cuts at: 1416 1416
Size: 4871
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 108 142 518 552 108
Size: 34 376 34 4427
Fragments arranged by size:
4427 376 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1317 2769 3530 4871 1317
Size: 1452 761 1341 1317
Fragments arranged by size:
1452 1341 1317 761
BclI T'GATC_A
Cuts at: 1520 2391 1520
Size: 871 4000
BglI GCCn_nnn'nGGC
Cuts at: 152 152
Size: 4871
BglII A'GATC_T
Cuts at: 2386 4423 2386
Size: 2037 2834
BlpI GC'TnA_GC
Cuts at: 172 1983 172
Size: 1811 3060
Bme1580I G_kGCm'C
Cuts at: 1675 2496 2589 3645 1675
Size: 821 93 1056 2901
Fragments arranged by size:
2901 1056 821 93
BmrI ACTGGGnnnn_n'
Cuts at: 615 2083 2164 2499 4643 615
Size: 1468 81 335 2144 843
Fragments arranged by size:
2144 1468 843 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3238 3238
Size: 4871
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 286 919 1811 181
Size: 105 633 892 3241
Fragments arranged by size:
3241 892 633 105
Bpu10I CC'TnA_GC
Cuts at: 636 1416 636
Size: 780 4091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 943 1305 1548 2047 2926 3418 3716 325
Size: 618 362 243 499 879 492 298 1480
Fragments arranged by size:
1480 879 618 499 492 362 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 349 754 4575 349
Size: 405 3821 645
Fragments arranged by size:
3821 645 405
BsaAI yAC'GTr
Cuts at: 1336 2224 1336
Size: 888 3983
BsaBI GATnn'nnATC
Cuts at: 6 2385 4422 6
Size: 2379 2037 455
Fragments arranged by size:
2379 2037 455
BsaHI Gr'CG_yC
Cuts at: 497 807 2302 2550 3252 4748 497
Size: 310 1495 248 702 1496 620
Fragments arranged by size:
1496 1495 702 620 310 248
BsaWI w'CCGG_w
Cuts at: 2060 2581 3259 3533 3680 4091 2060
Size: 521 678 274 147 411 2840
Fragments arranged by size:
2840 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1706 1736 1784 1814 1706
Size: 30 48 30 4763
Fragments arranged by size:
4763 48 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 325 451 763 907 1732 1783 1786 1789
Size: 102 126 312 144 825 51 3 3
Cuts at: 1789 223
Size: 3305
Fragments arranged by size:
3305 825 312 144 126 102 51 3
3
BseYI C'CCAG_C
Cuts at: 426 1022 1511 2296 3054 3631 4127 426
Size: 596 489 785 758 577 496 1170
Fragments arranged by size:
1170 785 758 596 577 496 489
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 545
Size: 4871
BsiEI CG_ry'CG
Cuts at: 104 324 2413 2459 3667 104
Size: 220 2089 46 1208 1308
Fragments arranged by size:
2089 1308 1208 220 46
BsiHKAI G_wGCw'C
Cuts at: 224 332 1885 2663 2853 3645 224
Size: 108 1553 778 190 792 1450
Fragments arranged by size:
1553 1450 792 778 190 108
BsmI GAATG_Cn'
Cuts at: 933 933
Size: 4871
BsmBI CGTCTCn'nnnn_
Cuts at: 262 262
Size: 4871
Bsp1286I G_dGCh'C
Cuts at: 178 224 332 1675 1885 2496 2589 2663 2853
Size: 46 108 1343 210 611 93 74 190
Cuts at: 2853 2915 3645 178
Size: 62 730 1404
Fragments arranged by size:
1404 1343 730 611 210 190 108 93
74 62 46
BspEI T'CCGG_A
Cuts at: 4091 4091
Size: 4871
BspHI T'CATG_A
Cuts at: 846 4047 846
Size: 3201 1670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 285 1921 2135 2437 2818 285
Size: 1636 214 302 381 2338
Fragments arranged by size:
2338 1636 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3163 3217 4701 4866 50
Size: 3113 54 1484 165 55
Fragments arranged by size:
3113 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2783 2783
Size: 4871
BsrFI r'CCGG_y
Cuts at: 230 334 2869 3050 3259 230
Size: 104 2535 181 209 1842
Fragments arranged by size:
2535 1842 209 181 104
BsrGI T'GTAC_A
Cuts at: 242 242
Size: 4871
BssHII G'CGCG_C
Cuts at: 281 380 405 281
Size: 99 25 4747
Fragments arranged by size:
4747 99 25
BssSI C'ACGA_G
Cuts at: 1665 3142 3500 1665
Size: 1477 358 3036
Fragments arranged by size:
3036 1477 358
BstAPI GCAn_nnn'nTGC
Cuts at: 553 2248 553
Size: 1695 3176
BstBI TT'CG_AA
Cuts at: 538 3232 538
Size: 2694 2177
BstYI r'GATC_y
Cuts at: 1 600 1896 2363 2386 2721 2967 3979 4065
Size: 599 1296 467 23 335 246 1012 86
Cuts at: 4065 4077 4423 1
Size: 12 346 449
Fragments arranged by size:
1296 1012 599 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 133 107
Size: 26 4845
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2821 3002 3165 2821
Size: 181 163 4527
Fragments arranged by size:
4527 181 163
BtsI GCAGTG_nn'
Cuts at: 2114 2114
Size: 4871
DraI TTT'AAA
Cuts at: 1746 1870 1746
Size: 124 4747
DraIII CAC_nnn'GTG
Cuts at: 568 568
Size: 4871
DrdI GACnn_nn'nnGTC
Cuts at: 1543 2577 3435 1543
Size: 1034 858 2979
Fragments arranged by size:
2979 1034 858
EaeI y'GGCC_r
Cuts at: 321 365 2456 2630 3021 3048 4393 321
Size: 44 2091 174 391 27 1345 799
Fragments arranged by size:
2091 1345 799 391 174 44 27
EagI C'GGCC_G
Cuts at: 321 2456 321
Size: 2135 2736
EarI CTCTTCn'nnn_
Cuts at: 740 1256 1351 1760 2894 3104 740
Size: 516 95 409 1134 210 2507
Fragments arranged by size:
2507 1134 516 409 210 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 457 1162 1401 3079 3389 3535 4681 457
Size: 705 239 1678 310 146 1146 647
Fragments arranged by size:
1678 1146 705 647 310 239 146
EcoICRI GAG'CTC
Cuts at: 222 330 1883 222
Size: 108 1553 3210
Fragments arranged by size:
3210 1553 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 286 625 919 1597 1781 1811 2696 3128
Size: 105 339 294 678 184 30 885 432
Cuts at: 3128 3875 4143 181
Size: 747 268 909
Fragments arranged by size:
909 885 747 678 432 339 294 268
184 105 30
EcoNI CCTnn'n_nnAGG
Cuts at: 712 712
Size: 4871
EcoO109I rG'GnC_Cy
Cuts at: 2010 2010
Size: 4871
EcoRI G'AATT_C
Cuts at: 534 1875 534
Size: 1341 3530
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1307 2890 1307
Size: 1583 3288
FspI TGC'GCA
Cuts at: 259 2652 259
Size: 2393 2478
HaeII r_GCGC'y
Cuts at: 349 500 2305 2553 3575 4554 349
Size: 151 1805 248 1022 979 666
Fragments arranged by size:
1805 1022 979 666 248 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 368 400 2713 2743 2745 2775 368
Size: 32 2313 30 2 30 2464
Fragments arranged by size:
2464 2313 32 30 30 2
HincII GTy'rAC
Cuts at: 966 1910 966
Size: 944 3927
HindIII A'AGCT_T
Cuts at: 508 508
Size: 4871
KasI G'GCGC_C
Cuts at: 496 2301 2549 496
Size: 1805 248 2818
Fragments arranged by size:
2818 1805 248
KpnI G_GTAC'C
Cuts at: 1891 1891
Size: 4871
MluI A'CGCG_T
Cuts at: 4304 4304
Size: 4871
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 605 3542 3726 605
Size: 2937 184 1750
Fragments arranged by size:
2937 1750 184
MscI TGG'CCA
Cuts at: 2632 2632
Size: 4871
MslI CAynn'nnrTG
Cuts at: 2860 2987 2860
Size: 127 4744
MspA1I CmG'CkG
Cuts at: 135 171 291 456 1176 1789 1982 2296 2656
Size: 36 120 165 720 613 193 314 360
Cuts at: 2656 3669 3914 135
Size: 1013 245 1092
Fragments arranged by size:
1092 1013 720 613 360 314 245 193
165 120 36
NaeI GCC'GGC
Cuts at: 3052 3052
Size: 4871
NarI GG'CG_CC
Cuts at: 497 2302 2550 497
Size: 1805 248 2818
Fragments arranged by size:
2818 1805 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4871
NgoMIV G'CCGG_C
Cuts at: 3050 3050
Size: 4871
NspI r_CATG'y
Cuts at: 741 1058 1122 1924 2424 3331 741
Size: 317 64 802 500 907 2281
Fragments arranged by size:
2281 907 802 500 317 64
PciI A'CATG_T
Cuts at: 3327 3327
Size: 4871
PfoI T'CCnGG_A
Cuts at: 197 197
Size: 4871
PmeI GTTT'AAAC
Cuts at: 1870 1870
Size: 4871
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1648 1680 4010 4042 1648
Size: 32 2330 32 2477
Fragments arranged by size:
2477 2330 32 32
PsiI TTA'TAA
Cuts at: 1553 1553
Size: 4871
PstI C_TGCA'G
Cuts at: 290 296 419 645 1918 2603 290
Size: 6 123 226 1273 685 2558
Fragments arranged by size:
2558 1273 685 226 123 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4871
PvuII CAG'CTG
Cuts at: 291 456 1176 1789 2296 2656 291
Size: 165 720 613 507 360 2506
Fragments arranged by size:
2506 720 613 507 360 165
RsrII CG'GwC_CG
Cuts at: 3066 3066
Size: 4871
SacI G_AGCT'C
Cuts at: 224 332 1885 224
Size: 108 1553 3210
Fragments arranged by size:
3210 1553 108
SacII CC_GC'GG
Cuts at: 136 136
Size: 4871
SalI G'TCGA_C
Cuts at: 1908 1908
Size: 4871
SapI GCTCTTCn'nnn_
Cuts at: 2894 3104 2894
Size: 210 4661
SbfI CC_TGCA'GG
Cuts at: 1918 1918
Size: 4871
ScaI AGT'ACT
Cuts at: 4388 4388
Size: 4871
SfcI C'TryA_G
Cuts at: 33 286 292 415 641 1036 1914 2599 3592
Size: 253 6 123 226 395 878 685 993
Cuts at: 3592 3783 33
Size: 191 1121
Fragments arranged by size:
1121 993 878 685 395 253 226 191
123 6
SfoI GGC'GCC
Cuts at: 498 2303 2551 498
Size: 1805 248 2818
Fragments arranged by size:
2818 1805 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4871
SmaI CCC'GGG
Cuts at: 1893 1893
Size: 4871
SmlI C'TyrA_G
Cuts at: 340 922 1320 1563 2026 2941 3433 3695 340
Size: 582 398 243 463 915 492 262 1516
Fragments arranged by size:
1516 915 582 492 463 398 262 243
SnaBI TAC'GTA
Cuts at: 1336 1336
Size: 4871
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4871
SphI G_CATG'C
Cuts at: 1122 1924 2424 1122
Size: 802 500 3569
Fragments arranged by size:
3569 802 500
StyI C'CwwG_G
Cuts at: 107 2005 2359 107
Size: 1898 354 2619
Fragments arranged by size:
2619 1898 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 373 2454 2587 3046 3255 373
Size: 2081 133 459 209 1989
Fragments arranged by size:
2081 1989 459 209 133
TatI w'GTAC_w
Cuts at: 242 824 1735 4386 242
Size: 582 911 2651 727
Fragments arranged by size:
2651 911 727 582
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4388 4420 4388
Size: 32 4839
Tth111I GACn'n_nGTC
Cuts at: 2668 2668
Size: 4871
XbaI T'CTAG_A
Cuts at: 1048 1902 1048
Size: 854 4017
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1508 2366 1508
Size: 858 4013
XmaI C'CCGG_G
Cuts at: 1891 1891
Size: 4871
XmnI GAAnn'nnTTC
Cuts at: 1067 1193 1067
Size: 126 4745
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BfrBI BmgBI BmtI BsiWI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI ZraI