Restriction map for
pF1KB6124
(Circular) (Six-base) MAPSORT of: pF1KB6124.seq Check: 2551 from: 1 to: 4991
pF1KB6124 4991 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 1166 2029 1166
Size: 863 4128
Acc65I G'GTAC_C
Cuts at: 193 402 2007 193
Size: 209 1605 3177
Fragments arranged by size:
3177 1605 209
AclI AA'CG_TT
Cuts at: 4831 4831
Size: 4991
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 860 2816 3248 3995 4263 860
Size: 1956 432 747 268 1588
Fragments arranged by size:
1956 1588 747 432 268
AflIII A'CryG_T
Cuts at: 2343 3447 4424 2343
Size: 1104 977 2910
Fragments arranged by size:
2910 1104 977
AgeI A'CCGG_T
Cuts at: 3379 3379
Size: 4991
AlwNI CAG_nnn'CTG
Cuts at: 631 1939 2296 3863 631
Size: 1308 357 1567 1759
Fragments arranged by size:
1759 1567 1308 357
ApaI G_GGCC'C
Cuts at: 166 166
Size: 4991
ApaLI G'TGCA_C
Cuts at: 3761 3761
Size: 4991
ApoI r'AATT_y
Cuts at: 1321 1995 4344 4600 1321
Size: 674 2349 256 1712
Fragments arranged by size:
2349 1712 674 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4991
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4991
AvaI C'yCGr_G
Cuts at: 159 752 1571 2011 159
Size: 593 819 440 3139
Fragments arranged by size:
3139 819 593 440
BamHI G'GATC_C
Cuts at: 2016 2016
Size: 4991
BanI G'GyrC_C
Cuts at: 193 402 487 2007 2421 2669 2704 193
Size: 209 85 1520 414 248 35 2480
Fragments arranged by size:
2480 1520 414 248 209 85 35
BanII G_rGCy'C
Cuts at: 166 2005 3035 166
Size: 1839 1030 2122
Fragments arranged by size:
2122 1839 1030
BbeI G_GCGC'C
Cuts at: 2425 2673 2425
Size: 248 4743
BbsI GAAGACnn'nnnn_
Cuts at: 627 896 1013 627
Size: 269 117 4605
Fragments arranged by size:
4605 269 117
BbvCI CC'TCA_GC
Cuts at: 1039 1607 1039
Size: 568 4423
BciVI GTATCCnnnnn_n'
Cuts at: 2889 3650 4991 2889
Size: 761 1341 2889
Fragments arranged by size:
2889 1341 761
BclI T'GATC_A
Cuts at: 2511 2511
Size: 4991
BglII A'GATC_T
Cuts at: 2506 4543 2506
Size: 2037 2954
BlpI GC'TnA_GC
Cuts at: 2103 2103
Size: 4991
Bme1580I G_kGCm'C
Cuts at: 166 2616 2709 3765 166
Size: 2450 93 1056 1392
Fragments arranged by size:
2450 1392 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1880 1954 2203 2284 2619 4763 1880
Size: 74 249 81 335 2144 2108
Fragments arranged by size:
2144 2108 335 249 81 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 747 1706 3358 747
Size: 959 1652 2380
Fragments arranged by size:
2380 1652 959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 1535 1647 1694 1727 851
Size: 684 112 47 33 4115
Fragments arranged by size:
4115 684 112 47 33
Bpu10I CC'TnA_GC
Cuts at: 451 1039 1607 1781 451
Size: 588 568 174 3661
Fragments arranged by size:
3661 588 568 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2167 3046 3538 3836 2167
Size: 879 492 298 3322
Fragments arranged by size:
3322 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4695 4695
Size: 4991
BsaAI yAC'GTr
Cuts at: 2344 2344
Size: 4991
BsaBI GATnn'nnATC
Cuts at: 6 2505 4542 6
Size: 2499 2037 455
Fragments arranged by size:
2499 2037 455
BsaHI Gr'CG_yC
Cuts at: 357 2422 2670 3372 4868 357
Size: 2065 248 702 1496 480
Fragments arranged by size:
2065 1496 702 480 248
BsaWI w'CCGG_w
Cuts at: 2180 2701 3379 3653 3800 4211 2180
Size: 521 678 274 147 411 2960
Fragments arranged by size:
2960 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 723 726 723
Size: 3 4988
BseYI C'CCAG_C
Cuts at: 1576 2416 3174 3751 4247 1576
Size: 840 758 577 496 2320
Fragments arranged by size:
2320 840 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 930 930
Size: 4991
BsiEI CG_ry'CG
Cuts at: 104 2533 2579 3787 104
Size: 2429 46 1208 1308
Fragments arranged by size:
2429 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 837 2005 2783 2973 3765 837
Size: 1168 778 190 792 2063
Fragments arranged by size:
2063 1168 792 778 190
BsmI GAATG_Cn'
Cuts at: 1068 1068
Size: 4991
Bsp1286I G_dGCh'C
Cuts at: 166 837 2005 2616 2709 2783 2973 3035 3765
Size: 671 1168 611 93 74 190 62 730
Cuts at: 3765 166
Size: 1392
Fragments arranged by size:
1392 1168 730 671 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4211 4211
Size: 4991
BspHI T'CATG_A
Cuts at: 1376 1916 4167 1376
Size: 540 2251 2200
Fragments arranged by size:
2251 2200 540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 182 701 919 2041 2255 2557 2938 182
Size: 519 218 1122 214 302 381 2235
Fragments arranged by size:
2235 1122 519 381 302 218 214
BsrBI CCG'CTC
Cuts at: 50 759 3283 3337 4821 4986 50
Size: 709 2524 54 1484 165 55
Fragments arranged by size:
2524 1484 709 165 55 54
BsrDI GCAATG_nn'
Cuts at: 425 1910 2903 425
Size: 1485 993 2513
Fragments arranged by size:
2513 1485 993
BsrFI r'CCGG_y
Cuts at: 2989 3170 3379 2989
Size: 181 209 4601
Fragments arranged by size:
4601 209 181
BssSI C'ACGA_G
Cuts at: 1680 3262 3620 1680
Size: 1582 358 3051
Fragments arranged by size:
3051 1582 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2368 2368
Size: 4991
BstBI TT'CG_AA
Cuts at: 3352 3352
Size: 4991
BstXI CCAn_nnnn'nTGG
Cuts at: 204 993 1443 204
Size: 789 450 3752
Fragments arranged by size:
3752 789 450
BstYI r'GATC_y
Cuts at: 1 641 2016 2483 2506 2841 3087 4099 4185
Size: 640 1375 467 23 335 246 1012 86
Cuts at: 4185 4197 4543 1
Size: 12 346 449
Fragments arranged by size:
1375 1012 640 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1975 107
Size: 1868 3123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1628 2941 3122 3285 1628
Size: 1313 181 163 3334
Fragments arranged by size:
3334 1313 181 163
BtsI GCAGTG_nn'
Cuts at: 2234 2234
Size: 4991
DraI TTT'AAA
Cuts at: 1326 1730 1990 1326
Size: 404 260 4327
Fragments arranged by size:
4327 404 260
DraIII CAC_nnn'GTG
Cuts at: 1223 1223
Size: 4991
DrdI GACnn_nn'nnGTC
Cuts at: 2697 3555 2697
Size: 858 4133
EaeI y'GGCC_r
Cuts at: 979 1978 2576 2750 3141 3168 4513 979
Size: 999 598 174 391 27 1345 1457
Fragments arranged by size:
1457 1345 999 598 391 174 27
EagI C'GGCC_G
Cuts at: 2576 2576
Size: 4991
EarI CTCTTCn'nnn_
Cuts at: 1710 3014 3224 1710
Size: 1304 210 3477
Fragments arranged by size:
3477 1304 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3199 3509 3655 4801 3199
Size: 310 146 1146 3389
Fragments arranged by size:
3389 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2003 2003
Size: 4991
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 860 1535 1647 1694 1727 2816 3248 3995
Size: 9 675 112 47 33 1089 432 747
Cuts at: 3995 4263 851
Size: 268 1579
Fragments arranged by size:
1579 1089 747 675 432 268 112 47
33 9
EcoO109I rG'GnC_Cy
Cuts at: 163 1455 1535 2130 163
Size: 1292 80 595 3024
Fragments arranged by size:
3024 1292 595 80
EcoRI G'AATT_C
Cuts at: 1995 1995
Size: 4991
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 633 3010 633
Size: 2377 2614
FspI TGC'GCA
Cuts at: 2772 2772
Size: 4991
HaeII r_GCGC'y
Cuts at: 2425 2673 3695 4674 2425
Size: 248 1022 979 2742
Fragments arranged by size:
2742 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 139 171 889 921 1858 1890 2833 2863 2865
Size: 32 718 32 937 32 943 30 2
Cuts at: 2865 2895 139
Size: 30 2235
Fragments arranged by size:
2235 943 937 718 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1959 2030 1959
Size: 71 4920
HindIII A'AGCT_T
Cuts at: 939 939
Size: 4991
KasI G'GCGC_C
Cuts at: 2421 2669 2421
Size: 248 4743
KpnI G_GTAC'C
Cuts at: 197 406 2011 197
Size: 209 1605 3177
Fragments arranged by size:
3177 1605 209
MfeI C'AATT_G
Cuts at: 1055 1055
Size: 4991
MluI A'CGCG_T
Cuts at: 4424 4424
Size: 4991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3662 3846 3662
Size: 184 4807
MscI TGG'CCA
Cuts at: 981 2752 981
Size: 1771 3220
MslI CAynn'nnrTG
Cuts at: 991 1073 1244 2980 3107 991
Size: 82 171 1736 127 2875
Fragments arranged by size:
2875 1736 171 127 82
MspA1I CmG'CkG
Cuts at: 1043 1983 2102 2416 2776 3789 4034 1043
Size: 940 119 314 360 1013 245 2000
Fragments arranged by size:
2000 1013 940 360 314 245 119
NaeI GCC'GGC
Cuts at: 3172 3172
Size: 4991
NarI GG'CG_CC
Cuts at: 2422 2670 2422
Size: 248 4743
NcoI C'CATG_G
Cuts at: 107 1975 107
Size: 1868 3123
NgoMIV G'CCGG_C
Cuts at: 3170 3170
Size: 4991
NspI r_CATG'y
Cuts at: 413 1506 2044 2544 3451 413
Size: 1093 538 500 907 1953
Fragments arranged by size:
1953 1093 907 538 500
PasI CC'CwG_GG
Cuts at: 808 808
Size: 4991
PciI A'CATG_T
Cuts at: 3447 3447
Size: 4991
PflMI CCAn_nnn'nTGG
Cuts at: 123 1494 123
Size: 1371 3620
PfoI T'CCnGG_A
Cuts at: 424 1442 1710 424
Size: 1018 268 3705
Fragments arranged by size:
3705 1018 268
PmeI GTTT'AAAC
Cuts at: 1990 1990
Size: 4991
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 4130
Size: 32 4959
PpuMI rG'GwC_Cy
Cuts at: 1455 1535 1455
Size: 80 4911
PspOMI G'GGCC_C
Cuts at: 162 162
Size: 4991
PstI C_TGCA'G
Cuts at: 2038 2723 2038
Size: 685 4306
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4991
PvuII CAG'CTG
Cuts at: 1043 2416 2776 1043
Size: 1373 360 3258
Fragments arranged by size:
3258 1373 360
RsrII CG'GwC_CG
Cuts at: 3186 3186
Size: 4991
SacI G_AGCT'C
Cuts at: 2005 2005
Size: 4991
SalI G'TCGA_C
Cuts at: 2028 2028
Size: 4991
SanDI GG'GwC_CC
Cuts at: 1455 1455
Size: 4991
SapI GCTCTTCn'nnn_
Cuts at: 3014 3224 3014
Size: 210 4781
SbfI CC_TGCA'GG
Cuts at: 2038 2038
Size: 4991
ScaI AGT'ACT
Cuts at: 4508 4508
Size: 4991
SfcI C'TryA_G
Cuts at: 33 651 852 2034 2719 3712 3903 33
Size: 618 201 1182 685 993 191 1121
Fragments arranged by size:
1182 1121 993 685 618 201 191
SfoI GGC'GCC
Cuts at: 2423 2671 2423
Size: 248 4743
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4991
SmaI CCC'GGG
Cuts at: 161 754 1573 2013 161
Size: 593 819 440 3139
Fragments arranged by size:
3139 819 593 440
SmlI C'TyrA_G
Cuts at: 2146 3061 3553 3815 2146
Size: 915 492 262 3322
Fragments arranged by size:
3322 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4991
SphI G_CATG'C
Cuts at: 1506 2044 2544 1506
Size: 538 500 3953
Fragments arranged by size:
3953 538 500
SspI AAT'ATT
Cuts at: 1234 1234
Size: 4991
StuI AGG'CCT
Cuts at: 873 873
Size: 4991
StyI C'CwwG_G
Cuts at: 107 306 1384 1391 1869 1975 2125 2479 107
Size: 199 1078 7 478 106 150 354 2619
Fragments arranged by size:
2619 1078 478 354 199 150 106 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 617 999 1936 2574 2707 3166 3375 617
Size: 382 937 638 133 459 209 2233
Fragments arranged by size:
2233 937 638 459 382 209 133
TatI w'GTAC_w
Cuts at: 4506 4506
Size: 4991
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4508 4540 4508
Size: 32 4959
Tth111I GACn'n_nGTC
Cuts at: 2788 2788
Size: 4991
XbaI T'CTAG_A
Cuts at: 2022 2022
Size: 4991
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2486 2486
Size: 4991
XmaI C'CCGG_G
Cuts at: 159 752 1571 2011 159
Size: 593 819 440 3139
Fragments arranged by size:
3139 819 593 440
XmnI GAAnn'nnTTC
Cuts at: 1235 1235
Size: 4991
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI