Restriction map for
pF1KB6116
(Circular) (Six-base) MAPSORT of: pF1KB6116.seq Check: 687 from: 1 to: 4859
pF1KB6116 4859 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 567 567
Size: 4859
AccI GT'mk_AC
Cuts at: 1799 1897 1799
Size: 98 4761
Acc65I G'GTAC_C
Cuts at: 251 1875 251
Size: 1624 3235
AclI AA'CG_TT
Cuts at: 4699 4699
Size: 4859
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1834 2684 3116 3863 4131 1834
Size: 850 432 747 268 2562
Fragments arranged by size:
2562 850 747 432 268
AflIII A'CryG_T
Cuts at: 1754 2211 3315 4292 1754
Size: 457 1104 977 2321
Fragments arranged by size:
2321 1104 977 457
AgeI A'CCGG_T
Cuts at: 3247 3247
Size: 4859
AleI CACnn'nnGTG
Cuts at: 370 370
Size: 4859
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 607 1546 607
Size: 939 3920
AlwNI CAG_nnn'CTG
Cuts at: 165 269 759 1095 2164 3731 165
Size: 104 490 336 1069 1567 1293
Fragments arranged by size:
1567 1293 1069 490 336 104
ApaI G_GGCC'C
Cuts at: 124 226 1380 124
Size: 102 1154 3603
Fragments arranged by size:
3603 1154 102
ApaLI G'TGCA_C
Cuts at: 889 3629 889
Size: 2740 2119
ApoI r'AATT_y
Cuts at: 1863 4212 4468 1863
Size: 2349 256 2254
Fragments arranged by size:
2349 2254 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4859
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4859
AvaI C'yCGr_G
Cuts at: 275 1078 1396 1508 1559 1879 275
Size: 803 318 112 51 320 3255
Fragments arranged by size:
3255 803 320 318 112 51
BamHI G'GATC_C
Cuts at: 1884 1884
Size: 4859
BanI G'GyrC_C
Cuts at: 251 395 655 670 1006 1102 1345 1875 2289
Size: 144 260 15 336 96 243 530 414
Cuts at: 2289 2537 2572 251
Size: 248 35 2538
Fragments arranged by size:
2538 530 414 336 260 248 243 144
96 35 15
BanII G_rGCy'C
Cuts at: 124 226 506 932 1380 1509 1808 1853 1873
Size: 102 280 426 448 129 299 45 20
Cuts at: 1873 2903 124
Size: 1030 2080
Fragments arranged by size:
2080 1030 448 426 299 280 129 102
45 20
BbeI G_GCGC'C
Cuts at: 399 2293 2541 399
Size: 1894 248 2717
Fragments arranged by size:
2717 1894 248
BbsI GAAGACnn'nnnn_
Cuts at: 572 1634 572
Size: 1062 3797
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 660 694 996 1030 660
Size: 34 302 34 4489
Fragments arranged by size:
4489 302 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2757 3518 4859 2757
Size: 761 1341 2757
Fragments arranged by size:
2757 1341 761
BclI T'GATC_A
Cuts at: 737 2379 737
Size: 1642 3217
BglI GCCn_nnn'nGGC
Cuts at: 214 428 1439 214
Size: 214 1011 3634
Fragments arranged by size:
3634 1011 214
BglII A'GATC_T
Cuts at: 2374 4411 2374
Size: 2037 2822
BlpI GC'TnA_GC
Cuts at: 1971 1971
Size: 4859
Bme1580I G_kGCm'C
Cuts at: 124 226 893 1380 1478 2484 2577 3633 124
Size: 102 667 487 98 1006 93 1056 1350
Fragments arranged by size:
1350 1056 1006 667 487 102 98 93
BmgBI CAC'GTC
Cuts at: 873 888 873
Size: 15 4844
BmrI ACTGGGnnnn_n'
Cuts at: 2071 2152 2487 4631 2071
Size: 81 335 2144 2299
Fragments arranged by size:
2299 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1689 3226 1689
Size: 1537 3322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 797 946 1190 797
Size: 149 244 4466
Fragments arranged by size:
4466 244 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1676 1792 2035 2914 3406 3704 1676
Size: 116 243 879 492 298 2831
Fragments arranged by size:
2831 879 492 298 243 116
BsaI GGTCTCn'nnnn_
Cuts at: 1270 1282 4563 1270
Size: 12 3281 1566
Fragments arranged by size:
3281 1566 12
BsaAI yAC'GTr
Cuts at: 2212 2212
Size: 4859
BsaBI GATnn'nnATC
Cuts at: 6 796 2373 4410 6
Size: 790 1577 2037 455
Fragments arranged by size:
2037 1577 790 455
BsaHI Gr'CG_yC
Cuts at: 396 453 1713 2290 2538 3240 4736 396
Size: 57 1260 577 248 702 1496 519
Fragments arranged by size:
1496 1260 702 577 519 248 57
BsaWI w'CCGG_w
Cuts at: 513 1662 2048 2569 3247 3521 3668 4079 513
Size: 1149 386 521 678 274 147 411 1293
Fragments arranged by size:
1293 1149 678 521 411 386 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 658 688 976 1006 1540 1570 658
Size: 30 288 30 534 30 3947
Fragments arranged by size:
3947 534 288 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 1681 1846 1861 130
Size: 1551 165 15 3128
Fragments arranged by size:
3128 1551 165 15
BseYI C'CCAG_C
Cuts at: 127 198 762 1349 2284 3042 3619 4115 127
Size: 71 564 587 935 758 577 496 871
Fragments arranged by size:
935 871 758 587 577 564 496 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 304 595 199
Size: 105 291 4463
Fragments arranged by size:
4463 291 105
BsiEI CG_ry'CG
Cuts at: 104 218 2401 2447 3655 104
Size: 114 2183 46 1208 1308
Fragments arranged by size:
2183 1308 1208 114 46
BsiHKAI G_wGCw'C
Cuts at: 586 893 932 1808 1853 1873 2651 2841 3633
Size: 307 39 876 45 20 778 190 792
Cuts at: 3633 586
Size: 1812
Fragments arranged by size:
1812 876 792 778 307 190 45 39
20
BsmBI CGTCTCn'nnnn_
Cuts at: 913 913
Size: 4859
Bsp1286I G_dGCh'C
Cuts at: 124 226 506 586 893 932 1380 1478 1509
Size: 102 280 80 307 39 448 98 31
Cuts at: 1509 1808 1853 1873 2484 2577 2651 2841 2903
Size: 299 45 20 611 93 74 190 62
Cuts at: 2903 3633 124
Size: 730 1350
Fragments arranged by size:
1350 730 611 448 307 299 280 190
102 98 93 80 74 62 45 39
31 20
BspEI T'CCGG_A
Cuts at: 1662 4079 1662
Size: 2417 2442
BspHI T'CATG_A
Cuts at: 4035 4035
Size: 4859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 567 1288 1909 2123 2425 2806 567
Size: 721 621 214 302 381 2620
Fragments arranged by size:
2620 721 621 381 302 214
BsrBI CCG'CTC
Cuts at: 50 447 1836 3151 3205 4689 4854 50
Size: 397 1389 1315 54 1484 165 55
Fragments arranged by size:
1484 1389 1315 397 165 55 54
BsrDI GCAATG_nn'
Cuts at: 586 1290 2771 586
Size: 704 1481 2674
Fragments arranged by size:
2674 1481 704
BsrFI r'CCGG_y
Cuts at: 2857 3038 3247 2857
Size: 181 209 4469
Fragments arranged by size:
4469 209 181
BssSI C'ACGA_G
Cuts at: 1843 3130 3488 1843
Size: 1287 358 3214
Fragments arranged by size:
3214 1287 358
BstAPI GCAn_nnn'nTGC
Cuts at: 165 2236 165
Size: 2071 2788
BstBI TT'CG_AA
Cuts at: 3220 3220
Size: 4859
BstXI CCAn_nnnn'nTGG
Cuts at: 384 652 988 384
Size: 268 336 4255
Fragments arranged by size:
4255 336 268
BstYI r'GATC_y
Cuts at: 1 1178 1884 2351 2374 2709 2955 3967 4053
Size: 1177 706 467 23 335 246 1012 86
Cuts at: 4053 4065 4411 1
Size: 12 346 449
Fragments arranged by size:
1177 1012 706 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 116 185 1491 116
Size: 69 1306 3484
Fragments arranged by size:
3484 1306 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2809 2990 3153 2809
Size: 181 163 4515
Fragments arranged by size:
4515 181 163
BtsI GCAGTG_nn'
Cuts at: 603 1530 2102 603
Size: 927 572 3360
Fragments arranged by size:
3360 927 572
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1281 1316 1281
Size: 35 4824
DraI TTT'AAA
Cuts at: 1858 1858
Size: 4859
DrdI GACnn_nn'nnGTC
Cuts at: 718 2565 3423 718
Size: 1847 858 2154
Fragments arranged by size:
2154 1847 858
EaeI y'GGCC_r
Cuts at: 215 374 1267 2444 2618 3009 3036 4381 215
Size: 159 893 1177 174 391 27 1345 693
Fragments arranged by size:
1345 1177 893 693 391 174 159 27
EagI C'GGCC_G
Cuts at: 215 2444 215
Size: 2229 2630
EarI CTCTTCn'nnn_
Cuts at: 1018 2882 3092 1018
Size: 1864 210 2785
Fragments arranged by size:
2785 1864 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3067 3377 3523 4669 3067
Size: 310 146 1146 3257
Fragments arranged by size:
3257 1146 310 146
EcoICRI GAG'CTC
Cuts at: 930 1806 1851 1871 930
Size: 876 45 20 3918
Fragments arranged by size:
3918 876 45 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 797 946 1190 1834 2684 3116 3863 4131 797
Size: 149 244 644 850 432 747 268 1525
Fragments arranged by size:
1525 850 747 644 432 268 244 149
EcoNI CCTnn'n_nnAGG
Cuts at: 1642 1642
Size: 4859
EcoO109I rG'GnC_Cy
Cuts at: 121 222 223 266 1376 1377 1605 1656 1731
Size: 101 1 43 1110 1 228 51 75
Cuts at: 1731 1998 121
Size: 267 2982
Fragments arranged by size:
2982 1110 267 228 101 75 51 43
1 1
EcoRI G'AATT_C
Cuts at: 1863 1863
Size: 4859
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2878 2878
Size: 4859
FspI TGC'GCA
Cuts at: 2640 2640
Size: 4859
HaeII r_GCGC'y
Cuts at: 399 2293 2541 3563 4542 399
Size: 1894 248 1022 979 716
Fragments arranged by size:
1894 1022 979 716 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 658 690 1163 1195 1540 1572 2701 2731 2733
Size: 32 473 32 345 32 1129 30 2
Cuts at: 2733 2763 658
Size: 30 2754
Fragments arranged by size:
2754 1129 473 345 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 441 1800 1898 441
Size: 1359 98 3402
Fragments arranged by size:
3402 1359 98
HindIII A'AGCT_T
Cuts at: 1297 1297
Size: 4859
KasI G'GCGC_C
Cuts at: 395 2289 2537 395
Size: 1894 248 2717
Fragments arranged by size:
2717 1894 248
KpnI G_GTAC'C
Cuts at: 255 1879 255
Size: 1624 3235
MluI A'CGCG_T
Cuts at: 4292 4292
Size: 4859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3530 3714 3530
Size: 184 4675
MscI TGG'CCA
Cuts at: 376 2620 376
Size: 2244 2615
MslI CAynn'nnrTG
Cuts at: 370 382 409 430 650 986 1447 2848 2975
Size: 12 27 21 220 336 461 1401 127
Cuts at: 2975 370
Size: 2254
Fragments arranged by size:
2254 1401 461 336 220 127 27 21
12
MspA1I CmG'CkG
Cuts at: 118 1353 1970 2284 2644 3657 3902 118
Size: 1235 617 314 360 1013 245 1075
Fragments arranged by size:
1235 1075 1013 617 360 314 245
NaeI GCC'GGC
Cuts at: 3040 3040
Size: 4859
NarI GG'CG_CC
Cuts at: 396 2290 2538 396
Size: 1894 248 2717
Fragments arranged by size:
2717 1894 248
NgoMIV G'CCGG_C
Cuts at: 3038 3038
Size: 4859
NspI r_CATG'y
Cuts at: 435 771 1446 1758 1912 2412 3319 435
Size: 336 675 312 154 500 907 1975
Fragments arranged by size:
1975 907 675 500 336 312 154
PasI CC'CwG_GG
Cuts at: 263 263
Size: 4859
PciI A'CATG_T
Cuts at: 1754 3315 1754
Size: 1561 3298
PfoI T'CCnGG_A
Cuts at: 620 774 620
Size: 154 4705
PmeI GTTT'AAAC
Cuts at: 1858 1858
Size: 4859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3998 4030 3998
Size: 32 4827
PpuMI rG'GwC_Cy
Cuts at: 1605 1656 1731 1605
Size: 51 75 4733
Fragments arranged by size:
4733 75 51
PspOMI G'GGCC_C
Cuts at: 120 222 1376 120
Size: 102 1154 3603
Fragments arranged by size:
3603 1154 102
PstI C_TGCA'G
Cuts at: 161 1100 1439 1906 2591 161
Size: 939 339 467 685 2429
Fragments arranged by size:
2429 939 685 467 339
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4859
PvuII CAG'CTG
Cuts at: 1353 2284 2644 1353
Size: 931 360 3568
Fragments arranged by size:
3568 931 360
RsrII CG'GwC_CG
Cuts at: 3054 3054
Size: 4859
SacI G_AGCT'C
Cuts at: 932 1808 1853 1873 932
Size: 876 45 20 3918
Fragments arranged by size:
3918 876 45 20
SacII CC_GC'GG
Cuts at: 119 119
Size: 4859
SalI G'TCGA_C
Cuts at: 1798 1896 1798
Size: 98 4761
SapI GCTCTTCn'nnn_
Cuts at: 2882 3092 2882
Size: 210 4649
SbfI CC_TGCA'GG
Cuts at: 1439 1906 1439
Size: 467 4392
ScaI AGT'ACT
Cuts at: 4376 4376
Size: 4859
SfcI C'TryA_G
Cuts at: 33 157 690 1096 1435 1902 2587 3580 3771
Size: 124 533 406 339 467 685 993 191
Cuts at: 3771 33
Size: 1121
Fragments arranged by size:
1121 993 685 533 467 406 339 191
124
SfoI GGC'GCC
Cuts at: 397 2291 2539 397
Size: 1894 248 2717
Fragments arranged by size:
2717 1894 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4859
SmaI CCC'GGG
Cuts at: 277 1080 1510 1561 1881 277
Size: 803 430 51 320 3255
Fragments arranged by size:
3255 803 430 320 51
SmlI C'TyrA_G
Cuts at: 1691 1807 2014 2929 3421 3683 1691
Size: 116 207 915 492 262 2867
Fragments arranged by size:
2867 915 492 262 207 116
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4859
SphI G_CATG'C
Cuts at: 1912 2412 1912
Size: 500 4359
StuI AGG'CCT
Cuts at: 422 856 1421 422
Size: 434 565 3860
Fragments arranged by size:
3860 565 434
StyI C'CwwG_G
Cuts at: 368 1993 2347 368
Size: 1625 354 2880
Fragments arranged by size:
2880 1625 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 376 431 1363 2442 2575 3034 3243 376
Size: 55 932 1079 133 459 209 1992
Fragments arranged by size:
1992 1079 932 459 209 133 55
TatI w'GTAC_w
Cuts at: 4374 4374
Size: 4859
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4376 4408 4376
Size: 32 4827
Tth111I GACn'n_nGTC
Cuts at: 634 907 1260 2656 634
Size: 273 353 1396 2837
Fragments arranged by size:
2837 1396 353 273
XbaI T'CTAG_A
Cuts at: 1890 1890
Size: 4859
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 478 1498 2354 478
Size: 1020 856 2983
Fragments arranged by size:
2983 1020 856
XmaI C'CCGG_G
Cuts at: 275 1078 1508 1559 1879 275
Size: 803 430 51 320 3255
Fragments arranged by size:
3255 803 430 320 51
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BfrBI BmtI Bpu10I BsiWI BsmI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PshAI PsiI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI