Restriction map for
pF1KB6113
(Circular) (Six-base) MAPSORT of: pF1KB6113.seq Check: 851 from: 1 to: 4973
pF1KB6113 4973 bp
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 1868 2011 1868
Size: 143 4830
Acc65I G'GTAC_C
Cuts at: 1989 1989
Size: 4973
AclI AA'CG_TT
Cuts at: 4813 4813
Size: 4973
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 611 641 1349 2798 3230 3977 4245 280
Size: 331 30 708 1449 432 747 268 1008
Fragments arranged by size:
1449 1008 747 708 432 331 268 30
AfeI AGC'GCT
Cuts at: 409 409
Size: 4973
AflIII A'CryG_T
Cuts at: 1863 2325 3429 4406 1863
Size: 462 1104 977 2430
Fragments arranged by size:
2430 1104 977 462
AgeI A'CCGG_T
Cuts at: 3361 3361
Size: 4973
AleI CACnn'nnGTG
Cuts at: 1201 1201
Size: 4973
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 489 521 489
Size: 32 4941
AlwNI CAG_nnn'CTG
Cuts at: 2278 3845 2278
Size: 1567 3406
ApaLI G'TGCA_C
Cuts at: 3743 3743
Size: 4973
ApoI r'AATT_y
Cuts at: 1663 1977 4326 4582 1663
Size: 314 2349 256 2054
Fragments arranged by size:
2349 2054 314 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4973
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4973
AvaI C'yCGr_G
Cuts at: 1993 1993
Size: 4973
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1096 1129 1806 1839 1096
Size: 33 677 33 4230
Fragments arranged by size:
4230 677 33 33
BamHI G'GATC_C
Cuts at: 1998 1998
Size: 4973
BanI G'GyrC_C
Cuts at: 456 1657 1989 2403 2651 2686 456
Size: 1201 332 414 248 35 2743
Fragments arranged by size:
2743 1201 414 332 248 35
BanII G_rGCy'C
Cuts at: 278 1196 1987 3017 278
Size: 918 791 1030 2234
Fragments arranged by size:
2234 1030 918 791
BbeI G_GCGC'C
Cuts at: 2407 2655 2407
Size: 248 4725
BbsI GAAGACnn'nnnn_
Cuts at: 599 1820 599
Size: 1221 3752
BciVI GTATCCnnnnn_n'
Cuts at: 1124 2871 3632 4973 1124
Size: 1747 761 1341 1124
Fragments arranged by size:
1747 1341 1124 761
BclI T'GATC_A
Cuts at: 1635 2493 1635
Size: 858 4115
BglI GCCn_nnn'nGGC
Cuts at: 220 220
Size: 4973
BglII A'GATC_T
Cuts at: 2488 4525 2488
Size: 2037 2936
BlpI GC'TnA_GC
Cuts at: 2085 2085
Size: 4973
Bme1580I G_kGCm'C
Cuts at: 540 2598 2691 3747 540
Size: 2058 93 1056 1766
Fragments arranged by size:
2058 1766 1056 93
BmgBI CAC'GTC
Cuts at: 1866 1866
Size: 4973
BmrI ACTGGGnnnn_n'
Cuts at: 1138 2185 2266 2601 4745 1138
Size: 1047 81 335 2144 1366
Fragments arranged by size:
2144 1366 1047 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1713 3340 1713
Size: 1627 3346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 535 1405 1908 371
Size: 164 870 503 3436
Fragments arranged by size:
3436 870 503 164
Bpu10I CC'TnA_GC
Cuts at: 1013 1013
Size: 4973
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2149 3028 3520 3818 2149
Size: 879 492 298 3304
Fragments arranged by size:
3304 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1471 1893 4677 1471
Size: 422 2784 1767
Fragments arranged by size:
2784 1767 422
BsaAI yAC'GTr
Cuts at: 2326 2326
Size: 4973
BsaBI GATnn'nnATC
Cuts at: 6 2487 4524 6
Size: 2481 2037 455
Fragments arranged by size:
2481 2037 455
BsaHI Gr'CG_yC
Cuts at: 2404 2652 3354 4850 2404
Size: 248 702 1496 2527
Fragments arranged by size:
2527 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1109 1825 2162 2683 3361 3635 3782 4193 1109
Size: 716 337 521 678 274 147 411 1889
Fragments arranged by size:
1889 716 678 521 411 337 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 489 519 1082 1112 489
Size: 30 563 30 4350
Fragments arranged by size:
4350 563 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1103 1336 1103
Size: 233 4740
BseYI C'CCAG_C
Cuts at: 410 1454 1485 2398 3156 3733 4229 410
Size: 1044 31 913 758 577 496 1154
Fragments arranged by size:
1154 1044 913 758 577 496 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1384 1384
Size: 4973
BsiEI CG_ry'CG
Cuts at: 104 2515 2561 3769 104
Size: 2411 46 1208 1308
Fragments arranged by size:
2411 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 278 1196 1427 1987 2765 2955 3747 278
Size: 918 231 560 778 190 792 1504
Fragments arranged by size:
1504 918 792 778 560 231 190
BsmI GAATG_Cn'
Cuts at: 564 564
Size: 4973
Bsp1286I G_dGCh'C
Cuts at: 278 540 1196 1427 1987 2598 2691 2765 2955
Size: 262 656 231 560 611 93 74 190
Cuts at: 2955 3017 3747 278
Size: 62 730 1504
Fragments arranged by size:
1504 730 656 611 560 262 231 190
93 74 62
BspEI T'CCGG_A
Cuts at: 4193 4193
Size: 4973
BspHI T'CATG_A
Cuts at: 4149 4149
Size: 4973
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2023 2237 2539 2920 2023
Size: 214 302 381 4076
Fragments arranged by size:
4076 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3265 3319 4803 4968 50
Size: 3215 54 1484 165 55
Fragments arranged by size:
3215 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 635 2885 635
Size: 2250 2723
BsrFI r'CCGG_y
Cuts at: 401 2971 3152 3361 401
Size: 2570 181 209 2013
Fragments arranged by size:
2570 2013 209 181
BsrGI T'GTAC_A
Cuts at: 1838 1838
Size: 4973
BssSI C'ACGA_G
Cuts at: 3244 3602 3244
Size: 358 4615
BstAPI GCAn_nnn'nTGC
Cuts at: 326 2350 326
Size: 2024 2949
BstBI TT'CG_AA
Cuts at: 3334 3334
Size: 4973
BstXI CCAn_nnnn'nTGG
Cuts at: 1084 1404 1084
Size: 320 4653
BstYI r'GATC_y
Cuts at: 1 1998 2465 2488 2823 3069 4081 4167 4179
Size: 1997 467 23 335 246 1012 86 12
Cuts at: 4179 4525 1
Size: 346 449
Fragments arranged by size:
1997 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 668 668
Size: 4973
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2923 3104 3267 2923
Size: 181 163 4629
Fragments arranged by size:
4629 181 163
BtsI GCAGTG_nn'
Cuts at: 679 1224 1884 2216 679
Size: 545 660 332 3436
Fragments arranged by size:
3436 660 545 332
DraI TTT'AAA
Cuts at: 186 1972 186
Size: 1786 3187
DrdI GACnn_nn'nnGTC
Cuts at: 1934 2679 3537 1934
Size: 745 858 3370
Fragments arranged by size:
3370 858 745
EaeI y'GGCC_r
Cuts at: 1912 2558 2732 3123 3150 4495 1912
Size: 646 174 391 27 1345 2390
Fragments arranged by size:
2390 1345 646 391 174 27
EagI C'GGCC_G
Cuts at: 2558 2558
Size: 4973
EarI CTCTTCn'nnn_
Cuts at: 581 689 1064 1301 1523 2996 3206 581
Size: 108 375 237 222 1473 210 2348
Fragments arranged by size:
2348 1473 375 237 222 210 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3181 3491 3637 4783 3181
Size: 310 146 1146 3371
Fragments arranged by size:
3371 1146 310 146
EcoICRI GAG'CTC
Cuts at: 276 1194 1985 276
Size: 918 791 3264
Fragments arranged by size:
3264 918 791
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 371 535 611 641 1349 1405 1908 2798
Size: 91 164 76 30 708 56 503 890
Cuts at: 2798 3230 3977 4245 280
Size: 432 747 268 1008
Fragments arranged by size:
1008 890 747 708 503 432 268 164
91 76 56 30
EcoNI CCTnn'n_nnAGG
Cuts at: 1387 1528 1387
Size: 141 4832
EcoO109I rG'GnC_Cy
Cuts at: 1392 2112 1392
Size: 720 4253
EcoRI G'AATT_C
Cuts at: 1977 1977
Size: 4973
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2992 2992
Size: 4973
FspI TGC'GCA
Cuts at: 2754 2754
Size: 4973
HaeII r_GCGC'y
Cuts at: 411 2407 2655 3677 4656 411
Size: 1996 248 1022 979 728
Fragments arranged by size:
1996 1022 979 728 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1717 1749 1866 1898 2815 2845 2847 2877 1717
Size: 32 117 32 917 30 2 30 3813
Fragments arranged by size:
3813 917 117 32 32 30 30 2
HincII GTy'rAC
Cuts at: 206 1939 2012 206
Size: 1733 73 3167
Fragments arranged by size:
3167 1733 73
HindIII A'AGCT_T
Cuts at: 520 520
Size: 4973
HpaI GTT'AAC
Cuts at: 206 206
Size: 4973
KasI G'GCGC_C
Cuts at: 2403 2651 2403
Size: 248 4725
KpnI G_GTAC'C
Cuts at: 1993 1993
Size: 4973
MluI A'CGCG_T
Cuts at: 4406 4406
Size: 4973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 574 3644 3828 574
Size: 3070 184 1719
Fragments arranged by size:
3070 1719 184
MscI TGG'CCA
Cuts at: 2734 2734
Size: 4973
MslI CAynn'nnrTG
Cuts at: 1201 1597 2962 3089 1201
Size: 396 1365 127 3085
Fragments arranged by size:
3085 1365 396 127
MspA1I CmG'CkG
Cuts at: 513 1368 1458 2084 2398 2758 3771 4016 513
Size: 855 90 626 314 360 1013 245 1470
Fragments arranged by size:
1470 1013 855 626 360 314 245 90
NaeI GCC'GGC
Cuts at: 403 3154 403
Size: 2751 2222
NarI GG'CG_CC
Cuts at: 2404 2652 2404
Size: 248 4725
NgoMIV G'CCGG_C
Cuts at: 401 3152 401
Size: 2751 2222
NspI r_CATG'y
Cuts at: 1494 1538 2026 2526 3433 1494
Size: 44 488 500 907 3034
Fragments arranged by size:
3034 907 500 488 44
PciI A'CATG_T
Cuts at: 3429 3429
Size: 4973
PfoI T'CCnGG_A
Cuts at: 125 740 907 125
Size: 615 167 4191
Fragments arranged by size:
4191 615 167
PmeI GTTT'AAAC
Cuts at: 1972 1972
Size: 4973
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 489 521 4112 4144 489
Size: 32 3591 32 1318
Fragments arranged by size:
3591 1318 32 32
PstI C_TGCA'G
Cuts at: 918 1476 2020 2705 918
Size: 558 544 685 3186
Fragments arranged by size:
3186 685 558 544
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4973
PvuII CAG'CTG
Cuts at: 513 1368 2398 2758 513
Size: 855 1030 360 2728
Fragments arranged by size:
2728 1030 855 360
RsrII CG'GwC_CG
Cuts at: 3168 3168
Size: 4973
SacI G_AGCT'C
Cuts at: 278 1196 1987 278
Size: 918 791 3264
Fragments arranged by size:
3264 918 791
SalI G'TCGA_C
Cuts at: 2010 2010
Size: 4973
SapI GCTCTTCn'nnn_
Cuts at: 2996 3206 2996
Size: 210 4763
SbfI CC_TGCA'GG
Cuts at: 2020 2020
Size: 4973
ScaI AGT'ACT
Cuts at: 700 4490 700
Size: 3790 1183
SfcI C'TryA_G
Cuts at: 33 914 1472 1710 2016 2701 3694 3885 33
Size: 881 558 238 306 685 993 191 1121
Fragments arranged by size:
1121 993 881 685 558 306 238 191
SfoI GGC'GCC
Cuts at: 2405 2653 2405
Size: 248 4725
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4973
SmaI CCC'GGG
Cuts at: 1995 1995
Size: 4973
SmlI C'TyrA_G
Cuts at: 2128 3043 3535 3797 2128
Size: 915 492 262 3304
Fragments arranged by size:
3304 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4973
SphI G_CATG'C
Cuts at: 2026 2526 2026
Size: 500 4473
StuI AGG'CCT
Cuts at: 1094 1094
Size: 4973
StyI C'CwwG_G
Cuts at: 944 2107 2461 944
Size: 1163 354 3456
Fragments arranged by size:
3456 1163 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 402 1164 1468 2556 2689 3148 3357 402
Size: 762 304 1088 133 459 209 2018
Fragments arranged by size:
2018 1088 762 459 304 209 133
TatI w'GTAC_w
Cuts at: 698 1640 1838 4488 698
Size: 942 198 2650 1183
Fragments arranged by size:
2650 1183 942 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 478 510 1082 1114 4490 4522 478
Size: 32 572 32 3376 32 929
Fragments arranged by size:
3376 929 572 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2770 2770
Size: 4973
XbaI T'CTAG_A
Cuts at: 2004 2004
Size: 4973
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 216 748 1569 2468 216
Size: 532 821 899 2721
Fragments arranged by size:
2721 899 821 532
XmaI C'CCGG_G
Cuts at: 1993 1993
Size: 4973
XmnI GAAnn'nnTTC
Cuts at: 168 860 168
Size: 692 4281
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI ApaI AscI AvrII
BbvCI BcgI BfrBI BmtI BsiWI BsmBI BssHII BstEII
BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI FspAI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI