Restriction map for
pF1KB6108
(Circular) (Six-base) MAPSORT of: pF1KB6108.seq Check: 1241 from: 1 to: 4964
pF1KB6108 4964 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1242 1776 1242
Size: 534 4430
AccI GT'mk_AC
Cuts at: 584 741 2002 584
Size: 157 1261 3546
Fragments arranged by size:
3546 1261 157
Acc65I G'GTAC_C
Cuts at: 1980 1980
Size: 4964
AclI AA'CG_TT
Cuts at: 4804 4804
Size: 4964
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1168 1459 1486 1908 2789 3221 3968 4236 1168
Size: 291 27 422 881 432 747 268 1896
Fragments arranged by size:
1896 881 747 432 422 291 268 27
AflIII A'CryG_T
Cuts at: 823 2316 3420 4397 823
Size: 1493 1104 977 1390
Fragments arranged by size:
1493 1390 1104 977
AgeI A'CCGG_T
Cuts at: 3352 3352
Size: 4964
AleI CACnn'nnGTG
Cuts at: 1285 1285
Size: 4964
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1520 1520
Size: 4964
AlwNI CAG_nnn'CTG
Cuts at: 1099 1170 1234 1500 2269 3836 1099
Size: 71 64 266 769 1567 2227
Fragments arranged by size:
2227 1567 769 266 71 64
ApaLI G'TGCA_C
Cuts at: 3734 3734
Size: 4964
ApoI r'AATT_y
Cuts at: 440 542 1084 1968 4317 4573 440
Size: 102 542 884 2349 256 831
Fragments arranged by size:
2349 884 831 542 256 102
AseI AT'TA_AT
Cuts at: 20 20
Size: 4964
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4964
AvaI C'yCGr_G
Cuts at: 1984 1984
Size: 4964
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1852 1885 1852
Size: 33 4931
BamHI G'GATC_C
Cuts at: 1989 1989
Size: 4964
BanI G'GyrC_C
Cuts at: 1980 2394 2642 2677 1980
Size: 414 248 35 4267
Fragments arranged by size:
4267 414 248 35
BanII G_rGCy'C
Cuts at: 1269 1558 1807 1814 1978 3008 1269
Size: 289 249 7 164 1030 3225
Fragments arranged by size:
3225 1030 289 249 164 7
BbeI G_GCGC'C
Cuts at: 2398 2646 2398
Size: 248 4716
BbsI GAAGACnn'nnnn_
Cuts at: 954 954
Size: 4964
BbvCI CC'TCA_GC
Cuts at: 746 1643 746
Size: 897 4067
BciVI GTATCCnnnnn_n'
Cuts at: 2862 3623 4964 2862
Size: 761 1341 2862
Fragments arranged by size:
2862 1341 761
BclI T'GATC_A
Cuts at: 1820 2484 1820
Size: 664 4300
BglI GCCn_nnn'nGGC
Cuts at: 121 121
Size: 4964
BglII A'GATC_T
Cuts at: 2479 4516 2479
Size: 2037 2927
BlpI GC'TnA_GC
Cuts at: 340 1242 1310 2076 340
Size: 902 68 766 3228
Fragments arranged by size:
3228 902 766 68
Bme1580I G_kGCm'C
Cuts at: 2589 2682 3738 2589
Size: 93 1056 3815
Fragments arranged by size:
3815 1056 93
BmgBI CAC'GTC
Cuts at: 207 207
Size: 4964
BmrI ACTGGGnnnn_n'
Cuts at: 544 713 2176 2257 2592 4736 544
Size: 169 1463 81 335 2144 772
Fragments arranged by size:
2144 1463 772 335 169 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3331 3331
Size: 4964
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 554 1277 1460 1831 1837 376
Size: 178 723 183 371 6 3503
Fragments arranged by size:
3503 723 371 183 178 6
Bpu10I CC'TnA_GC
Cuts at: 746 1643 746
Size: 897 4067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1439 1919 2140 3019 3511 3809 1439
Size: 480 221 879 492 298 2594
Fragments arranged by size:
2594 879 492 480 298 221
BsaI GGTCTCn'nnnn_
Cuts at: 1118 4668 1118
Size: 3550 1414
BsaAI yAC'GTr
Cuts at: 2317 2317
Size: 4964
BsaBI GATnn'nnATC
Cuts at: 6 2478 4515 6
Size: 2472 2037 455
Fragments arranged by size:
2472 2037 455
BsaHI Gr'CG_yC
Cuts at: 2395 2643 3345 4841 2395
Size: 248 702 1496 2518
Fragments arranged by size:
2518 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2153 2674 3352 3626 3773 4184 2153
Size: 521 678 274 147 411 2933
Fragments arranged by size:
2933 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1391 1421 1650 1680 1391
Size: 30 229 30 4675
Fragments arranged by size:
4675 229 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1106 1610 1777 1106
Size: 504 167 4293
Fragments arranged by size:
4293 504 167
BseYI C'CCAG_C
Cuts at: 2389 3147 3724 4220 2389
Size: 758 577 496 3133
Fragments arranged by size:
3133 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2506 2552 3760 104
Size: 2402 46 1208 1308
Fragments arranged by size:
2402 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1269 1547 1558 1978 2756 2946 3738 1269
Size: 278 11 420 778 190 792 2495
Fragments arranged by size:
2495 792 778 420 278 190 11
BsmI GAATG_Cn'
Cuts at: 469 602 469
Size: 133 4831
BsmBI CGTCTCn'nnnn_
Cuts at: 1676 1676
Size: 4964
Bsp1286I G_dGCh'C
Cuts at: 1269 1547 1558 1807 1814 1978 2589 2682 2756
Size: 278 11 249 7 164 611 93 74
Cuts at: 2756 2946 3008 3738 1269
Size: 190 62 730 2495
Fragments arranged by size:
2495 730 611 278 249 190 164 93
74 62 11 7
BspEI T'CCGG_A
Cuts at: 4184 4184
Size: 4964
BspHI T'CATG_A
Cuts at: 4140 4140
Size: 4964
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1242 1776 2014 2228 2530 2911 1242
Size: 534 238 214 302 381 3295
Fragments arranged by size:
3295 534 381 302 238 214
BsrBI CCG'CTC
Cuts at: 50 1736 3256 3310 4794 4959 50
Size: 1686 1520 54 1484 165 55
Fragments arranged by size:
1686 1520 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2876 2876
Size: 4964
BsrFI r'CCGG_y
Cuts at: 1046 1514 2962 3143 3352 1046
Size: 468 1448 181 209 2658
Fragments arranged by size:
2658 1448 468 209 181
BsrGI T'GTAC_A
Cuts at: 227 1143 1482 227
Size: 916 339 3709
Fragments arranged by size:
3709 916 339
BssSI C'ACGA_G
Cuts at: 1136 3235 3593 1136
Size: 2099 358 2507
Fragments arranged by size:
2507 2099 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1500 2341 1500
Size: 841 4123
BstBI TT'CG_AA
Cuts at: 3325 3325
Size: 4964
BstXI CCAn_nnnn'nTGG
Cuts at: 219 219
Size: 4964
BstYI r'GATC_y
Cuts at: 1 1989 2456 2479 2814 3060 4072 4158 4170
Size: 1988 467 23 335 246 1012 86 12
Cuts at: 4170 4516 1
Size: 346 449
Fragments arranged by size:
1988 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 742 742
Size: 4964
BtgI C'CryG_G
Cuts at: 107 116 1688 107
Size: 9 1572 3383
Fragments arranged by size:
3383 1572 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2914 3095 3258 2914
Size: 181 163 4620
Fragments arranged by size:
4620 181 163
BtsI GCAGTG_nn'
Cuts at: 1861 2207 1861
Size: 346 4618
DraI TTT'AAA
Cuts at: 1963 1963
Size: 4964
DraIII CAC_nnn'GTG
Cuts at: 629 629
Size: 4964
DrdI GACnn_nn'nnGTC
Cuts at: 2670 3528 2670
Size: 858 4106
EaeI y'GGCC_r
Cuts at: 209 237 1516 1526 2549 2723 3114 3141 4486
Size: 28 1279 10 1023 174 391 27 1345
Cuts at: 4486 209
Size: 687
Fragments arranged by size:
1345 1279 1023 687 391 174 28 27
10
EagI C'GGCC_G
Cuts at: 2549 2549
Size: 4964
EarI CTCTTCn'nnn_
Cuts at: 1371 1927 2987 3197 1371
Size: 556 1060 210 3138
Fragments arranged by size:
3138 1060 556 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3172 3482 3628 4774 3172
Size: 310 146 1146 3362
Fragments arranged by size:
3362 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1267 1556 1976 1267
Size: 289 420 4255
Fragments arranged by size:
4255 420 289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 554 1168 1277 1459 1460 1486 1831 1837
Size: 178 614 109 182 1 26 345 6
Cuts at: 1837 1908 2789 3221 3968 4236 376
Size: 71 881 432 747 268 1104
Fragments arranged by size:
1104 881 747 614 432 345 268 182
178 109 71 26 6 1
EcoNI CCTnn'n_nnAGG
Cuts at: 1751 1751
Size: 4964
EcoO109I rG'GnC_Cy
Cuts at: 396 1096 2103 396
Size: 700 1007 3257
Fragments arranged by size:
3257 1007 700
EcoRI G'AATT_C
Cuts at: 1968 1968
Size: 4964
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 320 913 2983 320
Size: 593 2070 2301
Fragments arranged by size:
2301 2070 593
FspI TGC'GCA
Cuts at: 2745 2745
Size: 4964
HaeII r_GCGC'y
Cuts at: 177 2398 2646 3668 4647 177
Size: 2221 248 1022 979 494
Fragments arranged by size:
2221 1022 979 494 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 416 448 1391 1423 1437 1469 2806 2836 2838
Size: 32 943 32 14 32 1337 30 2
Cuts at: 2838 2868 416
Size: 30 2512
Fragments arranged by size:
2512 1337 943 32 32 32 30 30
14 2
HincII GTy'rAC
Cuts at: 2003 2003
Size: 4964
HindIII A'AGCT_T
Cuts at: 895 1471 895
Size: 576 4388
KasI G'GCGC_C
Cuts at: 2394 2642 2394
Size: 248 4716
KpnI G_GTAC'C
Cuts at: 1984 1984
Size: 4964
MluI A'CGCG_T
Cuts at: 4397 4397
Size: 4964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 411 3635 3819 411
Size: 3224 184 1556
Fragments arranged by size:
3224 1556 184
MscI TGG'CCA
Cuts at: 211 2725 211
Size: 2514 2450
MslI CAynn'nnrTG
Cuts at: 328 1285 2953 3080 328
Size: 957 1668 127 2212
Fragments arranged by size:
2212 1668 957 127
MspA1I CmG'CkG
Cuts at: 1246 1500 2075 2389 2749 3762 4007 1246
Size: 254 575 314 360 1013 245 2203
Fragments arranged by size:
2203 1013 575 360 314 254 245
NaeI GCC'GGC
Cuts at: 3145 3145
Size: 4964
NarI GG'CG_CC
Cuts at: 2395 2643 2395
Size: 248 4716
NcoI C'CATG_G
Cuts at: 107 1688 107
Size: 1581 3383
NgoMIV G'CCGG_C
Cuts at: 3143 3143
Size: 4964
NspI r_CATG'y
Cuts at: 827 1507 2017 2517 3424 827
Size: 680 510 500 907 2367
Fragments arranged by size:
2367 907 680 510 500
PasI CC'CwG_GG
Cuts at: 1195 1195
Size: 4964
PciI A'CATG_T
Cuts at: 823 3420 823
Size: 2597 2367
PflMI CCAn_nnn'nTGG
Cuts at: 254 316 254
Size: 62 4902
PfoI T'CCnGG_A
Cuts at: 392 1293 1582 1853 392
Size: 901 289 271 3503
Fragments arranged by size:
3503 901 289 271
PmeI GTTT'AAAC
Cuts at: 1963 1963
Size: 4964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4103 4135 4103
Size: 32 4932
PpuMI rG'GwC_Cy
Cuts at: 396 1096 396
Size: 700 4264
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 624 656 624
Size: 32 4932
PstI C_TGCA'G
Cuts at: 173 2011 2696 173
Size: 1838 685 2441
Fragments arranged by size:
2441 1838 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4964
PvuII CAG'CTG
Cuts at: 1246 1500 2389 2749 1246
Size: 254 889 360 3461
Fragments arranged by size:
3461 889 360 254
RsrII CG'GwC_CG
Cuts at: 3159 3159
Size: 4964
SacI G_AGCT'C
Cuts at: 1269 1558 1978 1269
Size: 289 420 4255
Fragments arranged by size:
4255 420 289
SalI G'TCGA_C
Cuts at: 2001 2001
Size: 4964
SapI GCTCTTCn'nnn_
Cuts at: 2987 3197 2987
Size: 210 4754
SbfI CC_TGCA'GG
Cuts at: 2011 2011
Size: 4964
ScaI AGT'ACT
Cuts at: 4481 4481
Size: 4964
SfcI C'TryA_G
Cuts at: 33 169 411 1658 2007 2692 3685 3876 33
Size: 136 242 1247 349 685 993 191 1121
Fragments arranged by size:
1247 1121 993 685 349 242 191 136
SfoI GGC'GCC
Cuts at: 2396 2644 2396
Size: 248 4716
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4964
SmaI CCC'GGG
Cuts at: 1986 1986
Size: 4964
SmlI C'TyrA_G
Cuts at: 1454 1934 2119 3034 3526 3788 1454
Size: 480 185 915 492 262 2630
Fragments arranged by size:
2630 915 492 480 262 185
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4964
SphI G_CATG'C
Cuts at: 1507 2017 2517 1507
Size: 510 500 3954
Fragments arranged by size:
3954 510 500
StuI AGG'CCT
Cuts at: 1324 1324
Size: 4964
StyI C'CwwG_G
Cuts at: 107 1523 1574 1688 2098 2452 107
Size: 1416 51 114 410 354 2619
Fragments arranged by size:
2619 1416 410 354 114 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1037 1614 2547 2680 3139 3348 1037
Size: 577 933 133 459 209 2653
Fragments arranged by size:
2653 933 577 459 209 133
TatI w'GTAC_w
Cuts at: 227 1143 1222 1482 4479 227
Size: 916 79 260 2997 712
Fragments arranged by size:
2997 916 712 260 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4481 4513 4481
Size: 32 4932
Tth111I GACn'n_nGTC
Cuts at: 2761 2761
Size: 4964
XbaI T'CTAG_A
Cuts at: 1995 1995
Size: 4964
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 529 2459 114
Size: 415 1930 2619
Fragments arranged by size:
2619 1930 415
XmaI C'CCGG_G
Cuts at: 1984 1984
Size: 4964
XmnI GAAnn'nnTTC
Cuts at: 1171 1171
Size: 4964
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BcgI BfrBI BmtI BsgI BsiWI BssHII BstEII Bsu36I
ClaI CspCI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI