Restriction map for
pF1KB6102
(Circular) (Six-base) MAPSORT of: pF1KB6102.seq Check: 405 from: 1 to: 4955
pF1KB6102 4955 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1923 1923
Size: 4955
AccI GT'mk_AC
Cuts at: 1993 1993
Size: 4955
Acc65I G'GTAC_C
Cuts at: 1971 1971
Size: 4955
AclI AA'CG_TT
Cuts at: 4795 4795
Size: 4955
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 2780 3212 3959 4227 994
Size: 1786 432 747 268 1722
Fragments arranged by size:
1786 1722 747 432 268
AflIII A'CryG_T
Cuts at: 2307 3411 4388 2307
Size: 1104 977 2874
Fragments arranged by size:
2874 1104 977
AgeI A'CCGG_T
Cuts at: 3343 3343
Size: 4955
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 965 965
Size: 4955
AlwNI CAG_nnn'CTG
Cuts at: 747 1697 1776 2260 3827 747
Size: 950 79 484 1567 1875
Fragments arranged by size:
1875 1567 950 484 79
ApaLI G'TGCA_C
Cuts at: 823 3725 823
Size: 2902 2053
ApoI r'AATT_y
Cuts at: 607 1484 1959 4308 4564 607
Size: 877 475 2349 256 998
Fragments arranged by size:
2349 998 877 475 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4955
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
AvaI C'yCGr_G
Cuts at: 716 976 1975 716
Size: 260 999 3696
Fragments arranged by size:
3696 999 260
BamHI G'GATC_C
Cuts at: 1980 1980
Size: 4955
BanI G'GyrC_C
Cuts at: 216 382 1751 1971 2385 2633 2668 216
Size: 166 1369 220 414 248 35 2503
Fragments arranged by size:
2503 1369 414 248 220 166 35
BanII G_rGCy'C
Cuts at: 1792 1834 1969 2999 1792
Size: 42 135 1030 3748
Fragments arranged by size:
3748 1030 135 42
BbeI G_GCGC'C
Cuts at: 2389 2637 2389
Size: 248 4707
BbvCI CC'TCA_GC
Cuts at: 1324 1520 1324
Size: 196 4759
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 697 731 1880 1914 697
Size: 34 1149 34 3738
Fragments arranged by size:
3738 1149 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1423 2853 3614 4955 1423
Size: 1430 761 1341 1423
Fragments arranged by size:
1430 1423 1341 761
BclI T'GATC_A
Cuts at: 2475 2475
Size: 4955
BglI GCCn_nnn'nGGC
Cuts at: 1796 1796
Size: 4955
BglII A'GATC_T
Cuts at: 2470 4507 2470
Size: 2037 2918
BlpI GC'TnA_GC
Cuts at: 752 2067 752
Size: 1315 3640
Bme1580I G_kGCm'C
Cuts at: 387 827 965 1865 2580 2673 3729 387
Size: 440 138 900 715 93 1056 1613
Fragments arranged by size:
1613 1056 900 715 440 138 93
BmrI ACTGGGnnnn_n'
Cuts at: 735 839 1752 2167 2248 2583 4727 735
Size: 104 913 415 81 335 2144 963
Fragments arranged by size:
2144 963 913 415 335 104 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1365 3322 1365
Size: 1957 2998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 1335 326
Size: 1009 3946
Bpu10I CC'TnA_GC
Cuts at: 896 1324 1520 896
Size: 428 196 4331
Fragments arranged by size:
4331 428 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 2131 3010 3502 3800 655
Size: 1476 879 492 298 1810
Fragments arranged by size:
1810 1476 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1353 4659 1353
Size: 3306 1649
BsaAI yAC'GTr
Cuts at: 1434 2308 1434
Size: 874 4081
BsaBI GATnn'nnATC
Cuts at: 6 2469 4506 6
Size: 2463 2037 455
Fragments arranged by size:
2463 2037 455
BsaHI Gr'CG_yC
Cuts at: 2386 2634 3336 4832 2386
Size: 248 702 1496 2509
Fragments arranged by size:
2509 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2144 2665 3343 3617 3764 4175 2144
Size: 521 678 274 147 411 2924
Fragments arranged by size:
2924 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 876 906 876
Size: 30 4925
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 356 414 870 926 1654 1824 356
Size: 58 456 56 728 170 3487
Fragments arranged by size:
3487 728 456 170 58 56
BseYI C'CCAG_C
Cuts at: 187 1864 1869 2380 3138 3715 4211 187
Size: 1677 5 511 758 577 496 931
Fragments arranged by size:
1677 931 758 577 511 496 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 691 1394 422
Size: 269 703 3983
Fragments arranged by size:
3983 703 269
BsiEI CG_ry'CG
Cuts at: 104 1810 2497 2543 3751 104
Size: 1706 687 46 1208 1308
Fragments arranged by size:
1706 1308 1208 687 46
BsiHKAI G_wGCw'C
Cuts at: 477 827 1566 1969 2747 2937 3729 477
Size: 350 739 403 778 190 792 1703
Fragments arranged by size:
1703 792 778 739 403 350 190
Bsp1286I G_dGCh'C
Cuts at: 387 477 827 965 1566 1792 1834 1865 1969
Size: 90 350 138 601 226 42 31 104
Cuts at: 1969 2580 2673 2747 2937 2999 3729 387
Size: 611 93 74 190 62 730 1613
Fragments arranged by size:
1613 730 611 601 350 226 190 138
104 93 90 74 62 42 31
BspEI T'CCGG_A
Cuts at: 4175 4175
Size: 4955
BspHI T'CATG_A
Cuts at: 968 4131 968
Size: 3163 1792
BspMI ACCTGCnnnn'nnnn_
Cuts at: 689 1784 1923 2005 2219 2521 2902 689
Size: 1095 139 82 214 302 381 2742
Fragments arranged by size:
2742 1095 381 302 214 139 82
BsrBI CCG'CTC
Cuts at: 50 1749 3247 3301 4785 4950 50
Size: 1699 1498 54 1484 165 55
Fragments arranged by size:
1699 1498 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 354 417 534 782 1395 2867 354
Size: 63 117 248 613 1472 2442
Fragments arranged by size:
2442 1472 613 248 117 63
BsrFI r'CCGG_y
Cuts at: 1805 2953 3134 3343 1805
Size: 1148 181 209 3417
Fragments arranged by size:
3417 1148 209 181
BssSI C'ACGA_G
Cuts at: 3226 3584 3226
Size: 358 4597
BstAPI GCAn_nnn'nTGC
Cuts at: 793 1612 1697 2332 793
Size: 819 85 635 3416
Fragments arranged by size:
3416 819 635 85
BstBI TT'CG_AA
Cuts at: 3316 3316
Size: 4955
BstXI CCAn_nnnn'nTGG
Cuts at: 187 1108 187
Size: 921 4034
BstYI r'GATC_y
Cuts at: 1 262 1980 2447 2470 2805 3051 4063 4149
Size: 261 1718 467 23 335 246 1012 86
Cuts at: 4149 4161 4507 1
Size: 12 346 449
Fragments arranged by size:
1718 1012 467 449 346 335 261 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 153 153
Size: 4955
BtgI C'CryG_G
Cuts at: 107 1646 1794 107
Size: 1539 148 3268
Fragments arranged by size:
3268 1539 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 238 773 1883 2905 3086 3249 238
Size: 535 1110 1022 181 163 1944
Fragments arranged by size:
1944 1110 1022 535 181 163
BtsI GCAGTG_nn'
Cuts at: 708 1698 2198 708
Size: 990 500 3465
Fragments arranged by size:
3465 990 500
DraI TTT'AAA
Cuts at: 1954 1954
Size: 4955
DrdI GACnn_nn'nnGTC
Cuts at: 1103 2661 3519 1103
Size: 1558 858 2539
Fragments arranged by size:
2539 1558 858
EaeI y'GGCC_r
Cuts at: 126 722 1668 1782 1797 1807 2540 2714 3105
Size: 596 946 114 15 10 733 174 391
Cuts at: 3105 3132 4477 126
Size: 27 1345 604
Fragments arranged by size:
1345 946 733 604 596 391 174 114
27 15 10
EagI C'GGCC_G
Cuts at: 1807 2540 1807
Size: 733 4222
EarI CTCTTCn'nnn_
Cuts at: 832 857 1013 2978 3188 832
Size: 25 156 1965 210 2599
Fragments arranged by size:
2599 1965 210 156 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1836 3163 3473 3619 4765 1836
Size: 1327 310 146 1146 2026
Fragments arranged by size:
2026 1327 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1967 1967
Size: 4955
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 994 1335 2780 3212 3959 4227 326
Size: 668 341 1445 432 747 268 1054
Fragments arranged by size:
1445 1054 747 668 432 341 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1839 1839
Size: 4955
EcoO109I rG'GnC_Cy
Cuts at: 2094 2094
Size: 4955
EcoRI G'AATT_C
Cuts at: 607 1959 607
Size: 1352 3603
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2974 2974
Size: 4955
FseI GG_CCGG'CC
Cuts at: 1809 1809
Size: 4955
FspI TGC'GCA
Cuts at: 2736 2736
Size: 4955
HaeII r_GCGC'y
Cuts at: 1239 1260 2389 2637 3659 4638 1239
Size: 21 1129 248 1022 979 1556
Fragments arranged by size:
1556 1129 1022 979 248 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 831 863 1870 1902 2797 2827 2829 2859 831
Size: 32 1007 32 895 30 2 30 2927
Fragments arranged by size:
2927 1007 895 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1994 1994
Size: 4955
KasI G'GCGC_C
Cuts at: 2385 2633 2385
Size: 248 4707
KpnI G_GTAC'C
Cuts at: 1975 1975
Size: 4955
MfeI C'AATT_G
Cuts at: 454 599 887 454
Size: 145 288 4522
Fragments arranged by size:
4522 288 145
MluI A'CGCG_T
Cuts at: 4388 4388
Size: 4955
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3626 3810 3626
Size: 184 4771
MscI TGG'CCA
Cuts at: 128 1784 2716 128
Size: 1656 932 2367
Fragments arranged by size:
2367 1656 932
MslI CAynn'nnrTG
Cuts at: 820 830 2944 3071 820
Size: 10 2114 127 2704
Fragments arranged by size:
2704 2114 127 10
MspA1I CmG'CkG
Cuts at: 696 1812 2066 2380 2740 3753 3998 696
Size: 1116 254 314 360 1013 245 1653
Fragments arranged by size:
1653 1116 1013 360 314 254 245
NaeI GCC'GGC
Cuts at: 1807 3136 1807
Size: 1329 3626
NarI GG'CG_CC
Cuts at: 2386 2634 2386
Size: 248 4707
NcoI C'CATG_G
Cuts at: 107 107
Size: 4955
NgoMIV G'CCGG_C
Cuts at: 1805 3134 1805
Size: 1329 3626
NspI r_CATG'y
Cuts at: 1691 2008 2508 3415 1691
Size: 317 500 907 3231
Fragments arranged by size:
3231 907 500 317
PasI CC'CwG_GG
Cuts at: 1366 1366
Size: 4955
PciI A'CATG_T
Cuts at: 3411 3411
Size: 4955
PflMI CCAn_nnn'nTGG
Cuts at: 747 747
Size: 4955
PfoI T'CCnGG_A
Cuts at: 303 303
Size: 4955
PmeI GTTT'AAAC
Cuts at: 1954 1954
Size: 4955
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4094 4126 4094
Size: 32 4923
PstI C_TGCA'G
Cuts at: 695 1858 2002 2687 695
Size: 1163 144 685 2963
Fragments arranged by size:
2963 1163 685 144
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4955
PvuII CAG'CTG
Cuts at: 2380 2740 2380
Size: 360 4595
RsrII CG'GwC_CG
Cuts at: 3150 3150
Size: 4955
SacI G_AGCT'C
Cuts at: 1969 1969
Size: 4955
SalI G'TCGA_C
Cuts at: 1992 1992
Size: 4955
SapI GCTCTTCn'nnn_
Cuts at: 2978 3188 2978
Size: 210 4745
SbfI CC_TGCA'GG
Cuts at: 2002 2002
Size: 4955
ScaI AGT'ACT
Cuts at: 4472 4472
Size: 4955
SfcI C'TryA_G
Cuts at: 33 691 1820 1854 1998 2683 3676 3867 33
Size: 658 1129 34 144 685 993 191 1121
Fragments arranged by size:
1129 1121 993 685 658 191 144 34
SfoI GGC'GCC
Cuts at: 2387 2635 2387
Size: 248 4707
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4955
SmaI CCC'GGG
Cuts at: 978 1977 978
Size: 999 3956
SmlI C'TyrA_G
Cuts at: 670 2110 3025 3517 3779 670
Size: 1440 915 492 262 1846
Fragments arranged by size:
1846 1440 915 492 262
SnaBI TAC'GTA
Cuts at: 1434 1434
Size: 4955
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4955
SphI G_CATG'C
Cuts at: 2008 2508 2008
Size: 500 4455
StyI C'CwwG_G
Cuts at: 107 2089 2443 107
Size: 1982 354 2619
Fragments arranged by size:
2619 1982 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 327 1354 2538 2671 3130 3339 327
Size: 1027 1184 133 459 209 1943
Fragments arranged by size:
1943 1184 1027 459 209 133
TatI w'GTAC_w
Cuts at: 1503 4470 1503
Size: 2967 1988
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 224 256 624 656 876 908 4472 4504 224
Size: 32 368 32 220 32 3564 32 675
Fragments arranged by size:
3564 675 368 220 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2752 2752
Size: 4955
XbaI T'CTAG_A
Cuts at: 1986 1986
Size: 4955
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1762 2450 1762
Size: 688 4267
XmaI C'CCGG_G
Cuts at: 976 1975 976
Size: 999 3956
XmnI GAAnn'nnTTC
Cuts at: 764 764
Size: 4955
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI
StuI SwaI XhoI ZraI