Restriction map for
pF1KB6002
(Circular) (Six-base) MAPSORT of: pF1KB6002.seq Check: 5444 from: 1 to: 8171
pF1KB6002 8171 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 720 720
Size: 8171
AccI GT'mk_AC
Cuts at: 4991 5209 4991
Size: 218 7953
Acc65I G'GTAC_C
Cuts at: 5187 5187
Size: 8171
AclI AA'CG_TT
Cuts at: 2802 8011 2802
Size: 5209 2962
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1620 3426 3776 3791 4109 4746 4748 4886 5996
Size: 1806 350 15 318 637 2 138 1110
Cuts at: 5996 6428 7175 7443 1620
Size: 432 747 268 2348
Fragments arranged by size:
2348 1806 1110 747 637 432 350 318
268 138 15 2
AflIII A'CryG_T
Cuts at: 1426 2515 3192 3896 5523 6627 7604 1426
Size: 1089 677 704 1627 1104 977 1993
Fragments arranged by size:
1993 1627 1104 1089 977 704 677
AgeI A'CCGG_T
Cuts at: 6559 6559
Size: 8171
AleI CACnn'nnGTG
Cuts at: 3475 3475
Size: 8171
AlwNI CAG_nnn'CTG
Cuts at: 5476 7043 5476
Size: 1567 6604
ApaLI G'TGCA_C
Cuts at: 6941 6941
Size: 8171
ApoI r'AATT_y
Cuts at: 811 907 1288 2088 2192 2307 2405 2559 2953
Size: 96 381 800 104 115 98 154 394
Cuts at: 2953 3062 4448 4501 4946 5070 5175 7524 7780
Size: 109 1386 53 445 124 105 2349 256
Cuts at: 7780 811
Size: 1202
Fragments arranged by size:
2349 1386 1202 800 445 394 381 256
154 124 115 109 105 104 98 96
53
AseI AT'TA_AT
Cuts at: 20 3506 4004 20
Size: 3486 498 4187
Fragments arranged by size:
4187 3486 498
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8171
AvaI C'yCGr_G
Cuts at: 5191 5191
Size: 8171
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1693 1726 1693
Size: 33 8138
BamHI G'GATC_C
Cuts at: 927 1011 3718 5196 927
Size: 84 2707 1478 3902
Fragments arranged by size:
3902 2707 1478 84
BanI G'GyrC_C
Cuts at: 5187 5601 5849 5884 5187
Size: 414 248 35 7474
Fragments arranged by size:
7474 414 248 35
BanII G_rGCy'C
Cuts at: 481 2008 3969 4328 5185 6215 481
Size: 1527 1961 359 857 1030 2437
Fragments arranged by size:
2437 1961 1527 1030 857 359
BbeI G_GCGC'C
Cuts at: 5605 5853 5605
Size: 248 7923
BbsI GAAGACnn'nnnn_
Cuts at: 664 1288 1985 2824 4300 4476 664
Size: 624 697 839 1476 176 4359
Fragments arranged by size:
4359 1476 839 697 624 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1870 1904 1870
Size: 34 8137
BciVI GTATCCnnnnn_n'
Cuts at: 6069 6830 8171 6069
Size: 761 1341 6069
Fragments arranged by size:
6069 1341 761
BclI T'GATC_A
Cuts at: 2961 5691 2961
Size: 2730 5441
BfrBI ATG'CAT
Cuts at: 4845 4845
Size: 8171
BglII A'GATC_T
Cuts at: 3024 3531 3581 3603 4854 5686 7723 3024
Size: 507 50 22 1251 832 2037 3472
Fragments arranged by size:
3472 2037 1251 832 507 50 22
BlpI GC'TnA_GC
Cuts at: 4289 5283 4289
Size: 994 7177
Bme1580I G_kGCm'C
Cuts at: 2118 5796 5889 6945 2118
Size: 3678 93 1056 3344
Fragments arranged by size:
3678 3344 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 5383 5464 5799 7943 5383
Size: 81 335 2144 5611
Fragments arranged by size:
5611 2144 335 81
BmtI G_CTAG'C
Cuts at: 1006 1006
Size: 8171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6538 6538
Size: 8171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2149 4757 2149
Size: 2608 5563
Bpu10I CC'TnA_GC
Cuts at: 3751 3751
Size: 8171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 2648 3376 5136 5347 6226 6718 7016 427
Size: 2221 728 1760 211 879 492 298 1582
Fragments arranged by size:
2221 1760 1582 879 728 492 298 211
BsaI GGTCTCn'nnnn_
Cuts at: 1389 7875 1389
Size: 6486 1685
BsaAI yAC'GTr
Cuts at: 1427 5524 1427
Size: 4097 4074
BsaBI GATnn'nnATC
Cuts at: 6 326 1775 4846 5685 7722 6
Size: 320 1449 3071 839 2037 455
Fragments arranged by size:
3071 2037 1449 839 455 320
BsaHI Gr'CG_yC
Cuts at: 5602 5850 6552 8048 5602
Size: 248 702 1496 5725
Fragments arranged by size:
5725 1496 702 248
BsaWI w'CCGG_w
Cuts at: 159 5360 5881 6559 6833 6980 7391 159
Size: 5201 521 678 274 147 411 939
Fragments arranged by size:
5201 939 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 4778 4808 4778
Size: 30 8141
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1258 1988 2651 3293 4802 1258
Size: 730 663 642 1509 4627
Fragments arranged by size:
4627 1509 730 663 642
BseYI C'CCAG_C
Cuts at: 1475 5596 6354 6931 7427 1475
Size: 4121 758 577 496 2219
Fragments arranged by size:
4121 2219 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 5072 5072
Size: 8171
BsiEI CG_ry'CG
Cuts at: 104 5713 5759 6967 104
Size: 5609 46 1208 1308
Fragments arranged by size:
5609 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3207 5185 5963 6153 6945 3207
Size: 1978 778 190 792 4433
Fragments arranged by size:
4433 1978 792 778 190
BsmI GAATG_Cn'
Cuts at: 607 638 3486 4659 607
Size: 31 2848 1173 4119
Fragments arranged by size:
4119 2848 1173 31
BsmBI CGTCTCn'nnnn_
Cuts at: 3388 5119 3388
Size: 1731 6440
Bsp1286I G_dGCh'C
Cuts at: 481 2008 2118 3207 3969 4328 5185 5796 5889
Size: 1527 110 1089 762 359 857 611 93
Cuts at: 5889 5963 6153 6215 6945 481
Size: 74 190 62 730 1707
Fragments arranged by size:
1707 1527 1089 857 762 730 611 359
190 110 93 74 62
BspEI T'CCGG_A
Cuts at: 159 7391 159
Size: 7232 939
BspHI T'CATG_A
Cuts at: 1575 7347 1575
Size: 5772 2399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 720 4744 5221 5435 5737 6118 720
Size: 4024 477 214 302 381 2773
Fragments arranged by size:
4024 2773 477 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6463 6517 8001 8166 50
Size: 6413 54 1484 165 55
Fragments arranged by size:
6413 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1560 1649 2893 4250 6083 1560
Size: 89 1244 1357 1833 3648
Fragments arranged by size:
3648 1833 1357 1244 89
BsrFI r'CCGG_y
Cuts at: 6169 6350 6559 6169
Size: 181 209 7781
Fragments arranged by size:
7781 209 181
BsrGI T'GTAC_A
Cuts at: 3042 3168 3264 3042
Size: 126 96 7949
Fragments arranged by size:
7949 126 96
BssSI C'ACGA_G
Cuts at: 6442 6800 6442
Size: 358 7813
BstAPI GCAn_nnn'nTGC
Cuts at: 5548 5548
Size: 8171
BstBI TT'CG_AA
Cuts at: 1131 3882 6532 1131
Size: 2751 2650 2770
Fragments arranged by size:
2770 2751 2650
BstEII G'GTnAC_C
Cuts at: 4130 4130
Size: 8171
BstXI CCAn_nnnn'nTGG
Cuts at: 3108 3108
Size: 8171
BstYI r'GATC_y
Cuts at: 1 927 1011 2262 2974 3024 3531 3581 3603
Size: 926 84 1251 712 50 507 50 22
Cuts at: 3603 3718 4854 5196 5663 5686 6021 6267 7279
Size: 115 1136 342 467 23 335 246 1012
Cuts at: 7279 7365 7377 7723 1
Size: 86 12 346 449
Fragments arranged by size:
1251 1136 1012 926 712 507 467 449
346 342 335 246 115 86 84 50
50 23 22 12
BstZ17I GTA'TAC
Cuts at: 4992 4992
Size: 8171
Bsu36I CC'TnA_GG
Cuts at: 944 3685 944
Size: 2741 5430
BtgI C'CryG_G
Cuts at: 107 1688 107
Size: 1581 6590
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6121 6302 6465 6121
Size: 181 163 7827
Fragments arranged by size:
7827 181 163
BtsI GCAGTG_nn'
Cuts at: 2427 5414 2427
Size: 2987 5184
ClaI AT'CG_AT
Cuts at: 377 377
Size: 8171
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 164 199 3386 3421 164
Size: 35 3187 35 4914
Fragments arranged by size:
4914 3187 35 35
DraI TTT'AAA
Cuts at: 138 818 1124 1697 2271 2411 2502 3726 5170
Size: 680 306 573 574 140 91 1224 1444
Cuts at: 5170 138
Size: 3139
Fragments arranged by size:
3139 1444 1224 680 574 573 306 140
91
DrdI GACnn_nn'nnGTC
Cuts at: 4370 5877 6735 4370
Size: 1507 858 5806
Fragments arranged by size:
5806 1507 858
EaeI y'GGCC_r
Cuts at: 532 5756 5930 6321 6348 7693 532
Size: 5224 174 391 27 1345 1010
Fragments arranged by size:
5224 1345 1010 391 174 27
EagI C'GGCC_G
Cuts at: 5756 5756
Size: 8171
EarI CTCTTCn'nnn_
Cuts at: 974 1031 1220 1805 6194 6404 974
Size: 57 189 585 4389 210 2741
Fragments arranged by size:
4389 2741 585 210 189 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6379 6689 6835 7981 6379
Size: 310 146 1146 6569
Fragments arranged by size:
6569 1146 310 146
EcoICRI GAG'CTC
Cuts at: 5183 5183
Size: 8171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1620 2149 3426 3776 3791 4109 4746 4748 4757
Size: 529 1277 350 15 318 637 2 9
Cuts at: 4757 4886 5996 6428 7175 7443 1620
Size: 129 1110 432 747 268 2348
Fragments arranged by size:
2348 1277 1110 747 637 529 432 350
318 268 129 15 9 2
EcoNI CCTnn'n_nnAGG
Cuts at: 4772 4772
Size: 8171
EcoO109I rG'GnC_Cy
Cuts at: 5310 5310
Size: 8171
EcoRI G'AATT_C
Cuts at: 5175 5175
Size: 8171
EcoRV GAT'ATC
Cuts at: 119 375 900 2360 119
Size: 256 525 1460 5930
Fragments arranged by size:
5930 1460 525 256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 421 1136 2328 2746 4284 6190 421
Size: 715 1192 418 1538 1906 2402
Fragments arranged by size:
2402 1906 1538 1192 715 418
FspI TGC'GCA
Cuts at: 5952 5952
Size: 8171
HaeII r_GCGC'y
Cuts at: 2855 5605 5853 6875 7854 2855
Size: 2750 248 1022 979 3172
Fragments arranged by size:
3172 2750 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 243 275 1610 1642 6013 6043 6045 6075 243
Size: 32 1335 32 4371 30 2 30 2339
Fragments arranged by size:
4371 2339 1335 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2670 5210 2670
Size: 2540 5631
HindIII A'AGCT_T
Cuts at: 738 2465 2783 3047 738
Size: 1727 318 264 5862
Fragments arranged by size:
5862 1727 318 264
KasI G'GCGC_C
Cuts at: 5601 5849 5601
Size: 248 7923
KpnI G_GTAC'C
Cuts at: 5191 5191
Size: 8171
MfeI C'AATT_G
Cuts at: 2365 2365
Size: 8171
MluI A'CGCG_T
Cuts at: 7604 7604
Size: 8171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4993 6842 7026 4993
Size: 1849 184 6138
Fragments arranged by size:
6138 1849 184
MscI TGG'CCA
Cuts at: 534 5932 534
Size: 5398 2773
MslI CAynn'nnrTG
Cuts at: 1459 3475 4837 4840 6160 6287 1459
Size: 2016 1362 3 1320 127 3343
Fragments arranged by size:
3343 2016 1362 1320 127 3
MspA1I CmG'CkG
Cuts at: 131 5145 5282 5596 5956 6969 7214 131
Size: 5014 137 314 360 1013 245 1088
Fragments arranged by size:
5014 1088 1013 360 314 245 137
NaeI GCC'GGC
Cuts at: 6352 6352
Size: 8171
NarI GG'CG_CC
Cuts at: 5602 5850 5602
Size: 248 7923
NcoI C'CATG_G
Cuts at: 107 107
Size: 8171
NdeI CA'TA_TG
Cuts at: 1357 3996 4890 1357
Size: 2639 894 4638
Fragments arranged by size:
4638 2639 894
NgoMIV G'CCGG_C
Cuts at: 6350 6350
Size: 8171
NheI G'CTAG_C
Cuts at: 1002 1002
Size: 8171
NsiI A_TGCA'T
Cuts at: 4847 4847
Size: 8171
NspI r_CATG'y
Cuts at: 2519 3196 3900 3934 5224 5724 6631 2519
Size: 677 704 34 1290 500 907 4059
Fragments arranged by size:
4059 1290 907 704 677 500 34
PasI CC'CwG_GG
Cuts at: 1155 1155
Size: 8171
PciI A'CATG_T
Cuts at: 2515 3192 3896 6627 2515
Size: 677 704 2731 4059
Fragments arranged by size:
4059 2731 704 677
PfoI T'CCnGG_A
Cuts at: 675 2568 675
Size: 1893 6278
PmeI GTTT'AAAC
Cuts at: 5170 5170
Size: 8171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7310 7342 7310
Size: 32 8139
PsiI TTA'TAA
Cuts at: 311 1459 311
Size: 1148 7023
PstI C_TGCA'G
Cuts at: 1553 2058 4749 5218 5903 1553
Size: 505 2691 469 685 3821
Fragments arranged by size:
3821 2691 685 505 469
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8171
PvuII CAG'CTG
Cuts at: 5145 5596 5956 5145
Size: 451 360 7360
Fragments arranged by size:
7360 451 360
RsrII CG'GwC_CG
Cuts at: 6366 6366
Size: 8171
SacI G_AGCT'C
Cuts at: 5185 5185
Size: 8171
SalI G'TCGA_C
Cuts at: 5208 5208
Size: 8171
SapI GCTCTTCn'nnn_
Cuts at: 1805 6194 6404 1805
Size: 4389 210 3572
Fragments arranged by size:
4389 3572 210
SbfI CC_TGCA'GG
Cuts at: 5218 5218
Size: 8171
ScaI AGT'ACT
Cuts at: 768 7688 768
Size: 6920 1251
SfcI C'TryA_G
Cuts at: 33 650 874 1445 1549 1860 2054 2105 2614
Size: 617 224 571 104 311 194 51 509
Cuts at: 2614 4193 4745 5214 5899 6892 7083 33
Size: 1579 552 469 685 993 191 1121
Fragments arranged by size:
1579 1121 993 685 617 571 552 509
469 311 224 194 191 104 51
SfoI GGC'GCC
Cuts at: 5603 5851 5603
Size: 248 7923
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8171
SmaI CCC'GGG
Cuts at: 5193 5193
Size: 8171
SmlI C'TyrA_G
Cuts at: 406 2663 3391 5115 5326 6241 6733 6995 406
Size: 2257 728 1724 211 915 492 262 1582
Fragments arranged by size:
2257 1724 1582 915 728 492 262 211
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8171
SphI G_CATG'C
Cuts at: 5224 5724 5224
Size: 500 7671
SspI AAT'ATT
Cuts at: 1128 2843 3423 1128
Size: 1715 580 5876
Fragments arranged by size:
5876 1715 580
StuI AGG'CCT
Cuts at: 4063 4063
Size: 8171
StyI C'CwwG_G
Cuts at: 107 1015 1566 1936 5305 5659 107
Size: 908 551 370 3369 354 2619
Fragments arranged by size:
3369 2619 908 551 370 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 409 2837 3190 5071 5754 5887 6346 6555 409
Size: 2428 353 1881 683 133 459 209 2025
Fragments arranged by size:
2428 2025 1881 683 459 353 209 133
TatI w'GTAC_w
Cuts at: 766 802 1233 3042 3168 3264 3351 4690 5043
Size: 36 431 1809 126 96 87 1339 353
Cuts at: 5043 7686 766
Size: 2643 1251
Fragments arranged by size:
2643 1809 1339 1251 431 353 126 96
87 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4778 4810 4912 4944 7688 7720 4778
Size: 32 102 32 2744 32 5229
Fragments arranged by size:
5229 2744 102 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5968 5968
Size: 8171
XbaI T'CTAG_A
Cuts at: 5202 5202
Size: 8171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2089 2864 3703 3743 5666 2089
Size: 775 839 40 1923 4594
Fragments arranged by size:
4594 1923 839 775 40
XmaI C'CCGG_G
Cuts at: 5191 5191
Size: 8171
XmnI GAAnn'nnTTC
Cuts at: 1403 2311 2381 3242 3533 5074 5107 1403
Size: 908 70 861 291 1541 33 4467
Fragments arranged by size:
4467 1541 908 861 291 70 33
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NsiI NspI PasI PciI PfoI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BbvCI BglI BmgBI BsiWI BssHII DraIII FseI
FspAI HpaI NotI NruI PacI PflMI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI