Restriction map for
pF1KB5990
(Circular) (Six-base) MAPSORT of: pF1KB5990.seq Check: 1615 from: 1 to: 4940
pF1KB5990 4940 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 119 1318 119
Size: 1199 3741
AatII G_ACGT'C
Cuts at: 849 849
Size: 4940
AccI GT'mk_AC
Cuts at: 487 1978 487
Size: 1491 3449
Acc65I G'GTAC_C
Cuts at: 1956 1956
Size: 4940
AclI AA'CG_TT
Cuts at: 779 1542 4780 779
Size: 763 3238 939
Fragments arranged by size:
3238 939 763
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2765 3197 3944 4212 2765
Size: 432 747 268 3493
Fragments arranged by size:
3493 747 432 268
AflIII A'CryG_T
Cuts at: 2292 3396 4373 2292
Size: 1104 977 2859
Fragments arranged by size:
2859 1104 977
AgeI A'CCGG_T
Cuts at: 3328 3328
Size: 4940
AlwNI CAG_nnn'CTG
Cuts at: 867 2245 3812 867
Size: 1378 1567 1995
Fragments arranged by size:
1995 1567 1378
ApaLI G'TGCA_C
Cuts at: 3710 3710
Size: 4940
ApoI r'AATT_y
Cuts at: 583 1944 4293 4549 583
Size: 1361 2349 256 974
Fragments arranged by size:
2349 1361 974 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4940
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4940
AvaI C'yCGr_G
Cuts at: 1430 1960 1430
Size: 530 4410
AvrII C'CTAG_G
Cuts at: 1605 1605
Size: 4940
BamHI G'GATC_C
Cuts at: 1231 1965 1231
Size: 734 4206
BanI G'GyrC_C
Cuts at: 331 1956 2370 2618 2653 331
Size: 1625 414 248 35 2618
Fragments arranged by size:
2618 1625 414 248 35
BanII G_rGCy'C
Cuts at: 1954 2984 1954
Size: 1030 3910
BbeI G_GCGC'C
Cuts at: 335 2374 2622 335
Size: 2039 248 2653
Fragments arranged by size:
2653 2039 248
BbsI GAAGACnn'nnnn_
Cuts at: 1461 1819 1461
Size: 358 4582
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 859 893 859
Size: 34 4906
BciVI GTATCCnnnnn_n'
Cuts at: 2838 3599 4940 2838
Size: 761 1341 2838
Fragments arranged by size:
2838 1341 761
BclI T'GATC_A
Cuts at: 2460 2460
Size: 4940
BglII A'GATC_T
Cuts at: 2455 4492 2455
Size: 2037 2903
BlpI GC'TnA_GC
Cuts at: 1669 2052 1669
Size: 383 4557
Bme1580I G_kGCm'C
Cuts at: 2565 2658 3714 2565
Size: 93 1056 3791
Fragments arranged by size:
3791 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2152 2233 2568 4712 2152
Size: 81 335 2144 2380
Fragments arranged by size:
2380 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3307 3307
Size: 4940
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 1697 535
Size: 1162 3778
Bpu10I CC'TnA_GC
Cuts at: 664 664
Size: 4940
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2116 2995 3487 3785 2116
Size: 879 492 298 3271
Fragments arranged by size:
3271 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 185 1026 1715 4644 185
Size: 841 689 2929 481
Fragments arranged by size:
2929 841 689 481
BsaAI yAC'GTr
Cuts at: 2293 2293
Size: 4940
BsaBI GATnn'nnATC
Cuts at: 6 2454 4491 6
Size: 2448 2037 455
Fragments arranged by size:
2448 2037 455
BsaHI Gr'CG_yC
Cuts at: 332 846 2371 2619 3321 4817 332
Size: 514 1525 248 702 1496 455
Fragments arranged by size:
1525 1496 702 514 455 248
BsaWI w'CCGG_w
Cuts at: 2129 2650 3328 3602 3749 4160 2129
Size: 521 678 274 147 411 2909
Fragments arranged by size:
2909 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1169 1199 1408 1438 1169
Size: 30 209 30 4671
Fragments arranged by size:
4671 209 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 538 1514 538
Size: 976 3964
BseYI C'CCAG_C
Cuts at: 2365 3123 3700 4196 2365
Size: 758 577 496 3109
Fragments arranged by size:
3109 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 1107 1634 165
Size: 942 527 3471
Fragments arranged by size:
3471 942 527
BsiEI CG_ry'CG
Cuts at: 104 2482 2528 3736 104
Size: 2378 46 1208 1308
Fragments arranged by size:
2378 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1954 2732 2922 3714 1954
Size: 778 190 792 3180
Fragments arranged by size:
3180 792 778 190
BsmI GAATG_Cn'
Cuts at: 623 958 623
Size: 335 4605
Bsp1286I G_dGCh'C
Cuts at: 1954 2565 2658 2732 2922 2984 3714 1954
Size: 611 93 74 190 62 730 3180
Fragments arranged by size:
3180 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4160 4160
Size: 4940
BspHI T'CATG_A
Cuts at: 4116 4116
Size: 4940
BspMI ACCTGCnnnn'nnnn_
Cuts at: 119 234 1318 1990 2204 2506 2887 119
Size: 115 1084 672 214 302 381 2172
Fragments arranged by size:
2172 1084 672 381 302 214 115
BsrBI CCG'CTC
Cuts at: 50 3232 3286 4770 4935 50
Size: 3182 54 1484 165 55
Fragments arranged by size:
3182 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 522 2852 522
Size: 2330 2610
BsrFI r'CCGG_y
Cuts at: 2938 3119 3328 2938
Size: 181 209 4550
Fragments arranged by size:
4550 209 181
BssSI C'ACGA_G
Cuts at: 3211 3569 3211
Size: 358 4582
BstAPI GCAn_nnn'nTGC
Cuts at: 2317 2317
Size: 4940
BstBI TT'CG_AA
Cuts at: 3301 3301
Size: 4940
BstXI CCAn_nnnn'nTGG
Cuts at: 906 906
Size: 4940
BstYI r'GATC_y
Cuts at: 1 603 1231 1965 2432 2455 2790 3036 4048
Size: 602 628 734 467 23 335 246 1012
Cuts at: 4048 4134 4146 4492 1
Size: 86 12 346 449
Fragments arranged by size:
1012 734 628 602 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 488 488
Size: 4940
Bsu36I CC'TnA_GG
Cuts at: 1553 1553
Size: 4940
BtgI C'CryG_G
Cuts at: 107 1036 107
Size: 929 4011
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2890 3071 3234 2890
Size: 181 163 4596
Fragments arranged by size:
4596 181 163
BtsI GCAGTG_nn'
Cuts at: 792 815 1095 1293 1680 1763 2183 792
Size: 23 280 198 387 83 420 3549
Fragments arranged by size:
3549 420 387 280 198 83 23
DraI TTT'AAA
Cuts at: 1939 1939
Size: 4940
DraIII CAC_nnn'GTG
Cuts at: 1918 1918
Size: 4940
DrdI GACnn_nn'nnGTC
Cuts at: 2646 3504 2646
Size: 858 4082
EaeI y'GGCC_r
Cuts at: 1365 1721 2525 2699 3090 3117 4462 1365
Size: 356 804 174 391 27 1345 1843
Fragments arranged by size:
1843 1345 804 391 356 174 27
EagI C'GGCC_G
Cuts at: 2525 2525
Size: 4940
EarI CTCTTCn'nnn_
Cuts at: 503 533 713 2963 3173 503
Size: 30 180 2250 210 2270
Fragments arranged by size:
2270 2250 210 180 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3148 3458 3604 4750 3148
Size: 310 146 1146 3338
Fragments arranged by size:
3338 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1952 1952
Size: 4940
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 1697 2765 3197 3944 4212 535
Size: 1162 1068 432 747 268 1263
Fragments arranged by size:
1263 1162 1068 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1589 2079 1589
Size: 490 4450
EcoRI G'AATT_C
Cuts at: 583 1944 583
Size: 1361 3579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2959 2959
Size: 4940
FspI TGC'GCA
Cuts at: 127 2721 127
Size: 2594 2346
HaeII r_GCGC'y
Cuts at: 335 2374 2622 3644 4623 335
Size: 2039 248 1022 979 652
Fragments arranged by size:
2039 1022 979 652 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 745 777 1169 1201 1219 1251 1363 1392 1395
Size: 32 392 32 18 32 112 29 3
Cuts at: 1395 1424 1504 1509 1536 1541 2782 2812 2814
Size: 29 80 5 27 5 1241 30 2
Cuts at: 2814 2844 745
Size: 30 2841
Fragments arranged by size:
2841 1241 392 112 80 32 32 32
30 30 29 29 27 18 5 5
3 2
HincII GTy'rAC
Cuts at: 1979 1979
Size: 4940
KasI G'GCGC_C
Cuts at: 331 2370 2618 331
Size: 2039 248 2653
Fragments arranged by size:
2653 2039 248
KpnI G_GTAC'C
Cuts at: 1960 1960
Size: 4940
MluI A'CGCG_T
Cuts at: 4373 4373
Size: 4940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3611 3795 3611
Size: 184 4756
MscI TGG'CCA
Cuts at: 1367 1723 2701 1367
Size: 356 978 3606
Fragments arranged by size:
3606 978 356
MslI CAynn'nnrTG
Cuts at: 648 2929 3056 648
Size: 2281 127 2532
Fragments arranged by size:
2532 2281 127
MspA1I CmG'CkG
Cuts at: 179 538 555 2051 2365 2725 3738 3983 179
Size: 359 17 1496 314 360 1013 245 1136
Fragments arranged by size:
1496 1136 1013 360 359 314 245 17
NaeI GCC'GGC
Cuts at: 3121 3121
Size: 4940
NarI GG'CG_CC
Cuts at: 332 2371 2619 332
Size: 2039 248 2653
Fragments arranged by size:
2653 2039 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4940
NgoMIV G'CCGG_C
Cuts at: 3119 3119
Size: 4940
NspI r_CATG'y
Cuts at: 237 877 1993 2493 3400 237
Size: 640 1116 500 907 1777
Fragments arranged by size:
1777 1116 907 640 500
PciI A'CATG_T
Cuts at: 3396 3396
Size: 4940
PflMI CCAn_nnn'nTGG
Cuts at: 1139 1706 1847 1139
Size: 567 141 4232
Fragments arranged by size:
4232 567 141
PmeI GTTT'AAAC
Cuts at: 1939 1939
Size: 4940
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 4079
Size: 32 4908
PpuMI rG'GwC_Cy
Cuts at: 1589 1589
Size: 4940
PstI C_TGCA'G
Cuts at: 872 1315 1987 2672 872
Size: 443 672 685 3140
Fragments arranged by size:
3140 685 672 443
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4940
PvuII CAG'CTG
Cuts at: 538 555 2365 2725 538
Size: 17 1810 360 2753
Fragments arranged by size:
2753 1810 360 17
RsrII CG'GwC_CG
Cuts at: 3135 3135
Size: 4940
SacI G_AGCT'C
Cuts at: 1954 1954
Size: 4940
SalI G'TCGA_C
Cuts at: 1977 1977
Size: 4940
SanDI GG'GwC_CC
Cuts at: 1589 1589
Size: 4940
SapI GCTCTTCn'nnn_
Cuts at: 2963 3173 2963
Size: 210 4730
SbfI CC_TGCA'GG
Cuts at: 1315 1987 1315
Size: 672 4268
ScaI AGT'ACT
Cuts at: 1064 1337 4457 1064
Size: 273 3120 1547
Fragments arranged by size:
3120 1547 273
SfcI C'TryA_G
Cuts at: 33 372 868 1311 1499 1574 1640 1661 1983
Size: 339 496 443 188 75 66 21 322
Cuts at: 1983 2668 3661 3852 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 496 443 339 322 191
188 75 66 21
SfoI GGC'GCC
Cuts at: 333 2372 2620 333
Size: 2039 248 2653
Fragments arranged by size:
2653 2039 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4940
SmaI CCC'GGG
Cuts at: 1962 1962
Size: 4940
SmlI C'TyrA_G
Cuts at: 2095 3010 3502 3764 2095
Size: 915 492 262 3271
Fragments arranged by size:
3271 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4940
SphI G_CATG'C
Cuts at: 237 1993 2493 237
Size: 1756 500 2684
Fragments arranged by size:
2684 1756 500
StyI C'CwwG_G
Cuts at: 107 186 269 1024 1605 2074 2428 107
Size: 79 83 755 581 469 354 2619
Fragments arranged by size:
2619 755 581 469 354 83 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 700 1351 2523 2656 3115 3324 700
Size: 651 1172 133 459 209 2316
Fragments arranged by size:
2316 1172 651 459 209 133
TatI w'GTAC_w
Cuts at: 1062 1335 4455 1062
Size: 273 3120 1547
Fragments arranged by size:
3120 1547 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1408 1440 4457 4489 1408
Size: 32 3017 32 1859
Fragments arranged by size:
3017 1859 32 32
Tth111I GACn'n_nGTC
Cuts at: 2737 2737
Size: 4940
XbaI T'CTAG_A
Cuts at: 1971 1971
Size: 4940
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2435 2435
Size: 4940
XmaI C'CCGG_G
Cuts at: 1960 1960
Size: 4940
XmnI GAAnn'nnTTC
Cuts at: 215 215
Size: 4940
ZraI GAC'GTC
Cuts at: 847 847
Size: 4940
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
BaeI BbvCI BfrBI BglI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI