Restriction map for
pF1KB5983
(Circular) (Six-base) MAPSORT of: pF1KB5983.seq Check: 5000 from: 1 to: 4676
pF1KB5983 4676 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AccI GT'mk_AC
Cuts at: 1714 1714
Size: 4676
Acc65I G'GTAC_C
Cuts at: 1692 1692
Size: 4676
AclI AA'CG_TT
Cuts at: 4516 4516
Size: 4676
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 587 659 667 1558 2501 2933 3680 3948
Size: 105 72 8 891 943 432 747 268
Cuts at: 3948 482
Size: 1210
Fragments arranged by size:
1210 943 891 747 432 268 105 72
8
AfeI AGC'GCT
Cuts at: 415 1203 415
Size: 788 3888
AflIII A'CryG_T
Cuts at: 2028 3132 4109 2028
Size: 1104 977 2595
Fragments arranged by size:
2595 1104 977
AgeI A'CCGG_T
Cuts at: 1093 3064 1093
Size: 1971 2705
AlwNI CAG_nnn'CTG
Cuts at: 293 1149 1981 3548 293
Size: 856 832 1567 1421
Fragments arranged by size:
1567 1421 856 832
ApaLI G'TGCA_C
Cuts at: 3446 3446
Size: 4676
ApoI r'AATT_y
Cuts at: 130 136 973 1680 4029 4285 130
Size: 6 837 707 2349 256 521
Fragments arranged by size:
2349 837 707 521 256 6
AseI AT'TA_AT
Cuts at: 20 20
Size: 4676
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4676
AvaI C'yCGr_G
Cuts at: 1658 1696 1658
Size: 38 4638
BamHI G'GATC_C
Cuts at: 1701 1701
Size: 4676
BanI G'GyrC_C
Cuts at: 1692 2106 2354 2389 1692
Size: 414 248 35 3979
Fragments arranged by size:
3979 414 248 35
BanII G_rGCy'C
Cuts at: 1690 2720 1690
Size: 1030 3646
BbeI G_GCGC'C
Cuts at: 2110 2358 2110
Size: 248 4428
BbsI GAAGACnn'nnnn_
Cuts at: 1285 1285
Size: 4676
BbvCI CC'TCA_GC
Cuts at: 1211 1211
Size: 4676
BciVI GTATCCnnnnn_n'
Cuts at: 2574 3335 4676 2574
Size: 761 1341 2574
Fragments arranged by size:
2574 1341 761
BclI T'GATC_A
Cuts at: 2196 2196
Size: 4676
BglI GCCn_nnn'nGGC
Cuts at: 1633 1633
Size: 4676
BglII A'GATC_T
Cuts at: 2191 4228 2191
Size: 2037 2639
BlpI GC'TnA_GC
Cuts at: 1127 1788 1127
Size: 661 4015
Bme1580I G_kGCm'C
Cuts at: 1467 2301 2394 3450 1467
Size: 834 93 1056 2693
Fragments arranged by size:
2693 1056 834 93
BmrI ACTGGGnnnn_n'
Cuts at: 1031 1888 1969 2304 4448 1031
Size: 857 81 335 2144 1259
Fragments arranged by size:
2144 1259 857 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1047 1410 3043 1047
Size: 363 1633 2680
Fragments arranged by size:
2680 1633 363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 679
Size: 4676
Bpu10I CC'TnA_GC
Cuts at: 1211 1211
Size: 4676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1852 2731 3223 3521 1852
Size: 879 492 298 3007
Fragments arranged by size:
3007 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4380 4380
Size: 4676
BsaAI yAC'GTr
Cuts at: 2029 2029
Size: 4676
BsaBI GATnn'nnATC
Cuts at: 6 341 2190 4227 6
Size: 335 1849 2037 455
Fragments arranged by size:
2037 1849 455 335
BsaHI Gr'CG_yC
Cuts at: 2107 2355 3057 4553 2107
Size: 248 702 1496 2230
Fragments arranged by size:
2230 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1093 1865 2386 3064 3338 3485 3896 1093
Size: 772 521 678 274 147 411 1873
Fragments arranged by size:
1873 772 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 405 405
Size: 4676
BseYI C'CCAG_C
Cuts at: 293 843 2101 2859 3436 3932 293
Size: 550 1258 758 577 496 1037
Fragments arranged by size:
1258 1037 758 577 550 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 1249
Size: 4676
BsiEI CG_ry'CG
Cuts at: 104 2218 2264 3472 104
Size: 2114 46 1208 1308
Fragments arranged by size:
2114 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 397 1690 2468 2658 3450 397
Size: 1293 778 190 792 1623
Fragments arranged by size:
1623 1293 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 397 1467 1690 2301 2394 2468 2658 2720 3450
Size: 1070 223 611 93 74 190 62 730
Cuts at: 3450 397
Size: 1623
Fragments arranged by size:
1623 1070 730 611 223 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3896 3896
Size: 4676
BspHI T'CATG_A
Cuts at: 3852 3852
Size: 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 410 1726 1940 2242 2623 410
Size: 1316 214 302 381 2463
Fragments arranged by size:
2463 1316 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2968 3022 4506 4671 50
Size: 2918 54 1484 165 55
Fragments arranged by size:
2918 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1138 2588 1138
Size: 1450 3226
BsrFI r'CCGG_y
Cuts at: 1093 1223 2674 2855 3064 1093
Size: 130 1451 181 209 2705
Fragments arranged by size:
2705 1451 209 181 130
BssSI C'ACGA_G
Cuts at: 2947 3305 2947
Size: 358 4318
BstAPI GCAn_nnn'nTGC
Cuts at: 2053 2053
Size: 4676
BstBI TT'CG_AA
Cuts at: 3037 3037
Size: 4676
BstEII G'GTnAC_C
Cuts at: 1429 1429
Size: 4676
BstXI CCAn_nnnn'nTGG
Cuts at: 277 277
Size: 4676
BstYI r'GATC_y
Cuts at: 1 251 1701 2168 2191 2526 2772 3784 3870
Size: 250 1450 467 23 335 246 1012 86
Cuts at: 3870 3882 4228 1
Size: 12 346 449
Fragments arranged by size:
1450 1012 467 449 346 335 250 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 388 388
Size: 4676
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2626 2807 2970 2626
Size: 181 163 4332
Fragments arranged by size:
4332 181 163
BtsI GCAGTG_nn'
Cuts at: 1919 1919
Size: 4676
DraI TTT'AAA
Cuts at: 135 1675 135
Size: 1540 3136
DrdI GACnn_nn'nnGTC
Cuts at: 2382 3240 2382
Size: 858 3818
EaeI y'GGCC_r
Cuts at: 2261 2435 2826 2853 4198 2261
Size: 174 391 27 1345 2739
Fragments arranged by size:
2739 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2261 2261
Size: 4676
EarI CTCTTCn'nnn_
Cuts at: 1264 2699 2909 1264
Size: 1435 210 3031
Fragments arranged by size:
3031 1435 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1031 1230 2884 3194 3340 4486 1031
Size: 199 1654 310 146 1146 1221
Fragments arranged by size:
1654 1221 1146 310 199 146
EcoICRI GAG'CTC
Cuts at: 1688 1688
Size: 4676
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 587 659 667 679 1558 2501 2933 3680
Size: 105 72 8 12 879 943 432 747
Cuts at: 3680 3948 482
Size: 268 1210
Fragments arranged by size:
1210 943 879 747 432 268 105 72
12 8
EcoNI CCTnn'n_nnAGG
Cuts at: 868 868
Size: 4676
EcoO109I rG'GnC_Cy
Cuts at: 1389 1815 1389
Size: 426 4250
EcoRI G'AATT_C
Cuts at: 1680 1680
Size: 4676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1536 2695 1536
Size: 1159 3517
FspI TGC'GCA
Cuts at: 2457 2457
Size: 4676
HaeII r_GCGC'y
Cuts at: 417 1205 1224 2110 2358 3380 4359 417
Size: 788 19 886 248 1022 979 734
Fragments arranged by size:
1022 979 886 788 734 248 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 472 504 780 812 2518 2548 2550 2580 472
Size: 32 276 32 1706 30 2 30 2568
Fragments arranged by size:
2568 1706 276 32 32 30 30 2
HincII GTy'rAC
Cuts at: 861 1715 861
Size: 854 3822
HindIII A'AGCT_T
Cuts at: 1402 1402
Size: 4676
KasI G'GCGC_C
Cuts at: 2106 2354 2106
Size: 248 4428
KpnI G_GTAC'C
Cuts at: 1696 1696
Size: 4676
MluI A'CGCG_T
Cuts at: 4109 4109
Size: 4676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 262 3347 3531 262
Size: 3085 184 1407
Fragments arranged by size:
3085 1407 184
MscI TGG'CCA
Cuts at: 2437 2437
Size: 4676
MslI CAynn'nnrTG
Cuts at: 275 829 2665 2792 275
Size: 554 1836 127 2159
Fragments arranged by size:
2159 1836 554 127
MspA1I CmG'CkG
Cuts at: 657 1787 2101 2461 3474 3719 657
Size: 1130 314 360 1013 245 1614
Fragments arranged by size:
1614 1130 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1225 2857 1225
Size: 1632 3044
NarI GG'CG_CC
Cuts at: 2107 2355 2107
Size: 248 4428
NdeI CA'TA_TG
Cuts at: 936 936
Size: 4676
NgoMIV G'CCGG_C
Cuts at: 1223 2855 1223
Size: 1632 3044
NspI r_CATG'y
Cuts at: 820 1251 1729 2229 3136 820
Size: 431 478 500 907 2360
Fragments arranged by size:
2360 907 500 478 431
PciI A'CATG_T
Cuts at: 3132 3132
Size: 4676
PfoI T'CCnGG_A
Cuts at: 1396 1396
Size: 4676
PmeI GTTT'AAAC
Cuts at: 1675 1675
Size: 4676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 3815
Size: 32 4644
PpuMI rG'GwC_Cy
Cuts at: 1389 1389
Size: 4676
PstI C_TGCA'G
Cuts at: 421 1723 2408 421
Size: 1302 685 2689
Fragments arranged by size:
2689 1302 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4676
PvuII CAG'CTG
Cuts at: 657 2101 2461 657
Size: 1444 360 2872
Fragments arranged by size:
2872 1444 360
RsrII CG'GwC_CG
Cuts at: 2871 2871
Size: 4676
SacI G_AGCT'C
Cuts at: 1690 1690
Size: 4676
SalI G'TCGA_C
Cuts at: 1713 1713
Size: 4676
SapI GCTCTTCn'nnn_
Cuts at: 1264 2699 2909 1264
Size: 1435 210 3031
Fragments arranged by size:
3031 1435 210
SbfI CC_TGCA'GG
Cuts at: 1723 1723
Size: 4676
ScaI AGT'ACT
Cuts at: 4193 4193
Size: 4676
SfcI C'TryA_G
Cuts at: 33 377 417 510 626 1719 2404 3397 3588
Size: 344 40 93 116 1093 685 993 191
Cuts at: 3588 33
Size: 1121
Fragments arranged by size:
1121 1093 993 685 344 191 116 93
40
SfoI GGC'GCC
Cuts at: 2108 2356 2108
Size: 248 4428
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4676
SgrAI Cr'CCGG_yG
Cuts at: 1093 1093
Size: 4676
SmaI CCC'GGG
Cuts at: 1660 1698 1660
Size: 38 4638
SmlI C'TyrA_G
Cuts at: 1831 2746 3238 3500 1831
Size: 915 492 262 3007
Fragments arranged by size:
3007 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4676
SphI G_CATG'C
Cuts at: 1251 1729 2229 1251
Size: 478 500 3698
Fragments arranged by size:
3698 500 478
StuI AGG'CCT
Cuts at: 452 792 452
Size: 340 4336
StyI C'CwwG_G
Cuts at: 182 1506 1810 2164 182
Size: 1324 304 354 2694
Fragments arranged by size:
2694 1324 354 304
SwaI ATTT'AAAT
Cuts at: 135 135
Size: 4676
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2259 2392 2851 3060 2259
Size: 133 459 209 3875
Fragments arranged by size:
3875 459 209 133
TatI w'GTAC_w
Cuts at: 4191 4191
Size: 4676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4193 4225 4193
Size: 32 4644
Tth111I GACn'n_nGTC
Cuts at: 439 966 2473 439
Size: 527 1507 2642
Fragments arranged by size:
2642 1507 527
XbaI T'CTAG_A
Cuts at: 1707 1707
Size: 4676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2171 2171
Size: 4676
XmaI C'CCGG_G
Cuts at: 1658 1696 1658
Size: 38 4638
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NcoI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI SspI XhoI XmnI ZraI