Restriction map for
pF1KB5981
(Circular) (Six-base) MAPSORT of: pF1KB5981.seq Check: 7763 from: 1 to: 4496
pF1KB5981 4496 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 741 1534 741
Size: 793 3703
Acc65I G'GTAC_C
Cuts at: 1512 1512
Size: 4496
AclI AA'CG_TT
Cuts at: 4336 4336
Size: 4496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 339 786 1206 1290 1374 1454 2321 2753
Size: 138 447 420 84 84 80 867 432
Cuts at: 2753 3500 3768 201
Size: 747 268 929
Fragments arranged by size:
929 867 747 447 432 420 268 138
84 84 80
AflIII A'CryG_T
Cuts at: 526 662 1848 2952 3929 526
Size: 136 1186 1104 977 1093
Fragments arranged by size:
1186 1104 1093 977 136
AgeI A'CCGG_T
Cuts at: 2884 2884
Size: 4496
AlwNI CAG_nnn'CTG
Cuts at: 1801 3368 1801
Size: 1567 2929
ApaLI G'TGCA_C
Cuts at: 3266 3266
Size: 4496
ApoI r'AATT_y
Cuts at: 278 1256 1424 1500 3849 4105 278
Size: 978 168 76 2349 256 669
Fragments arranged by size:
2349 978 669 256 168 76
AseI AT'TA_AT
Cuts at: 20 20
Size: 4496
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
AvaI C'yCGr_G
Cuts at: 1516 1516
Size: 4496
BamHI G'GATC_C
Cuts at: 171 1521 171
Size: 1350 3146
BanI G'GyrC_C
Cuts at: 1512 1926 2174 2209 1512
Size: 414 248 35 3799
Fragments arranged by size:
3799 414 248 35
BanII G_rGCy'C
Cuts at: 1510 2540 1510
Size: 1030 3466
BbeI G_GCGC'C
Cuts at: 1930 2178 1930
Size: 248 4248
BbsI GAAGACnn'nnnn_
Cuts at: 257 272 663 1083 257
Size: 15 391 420 3670
Fragments arranged by size:
3670 420 391 15
BciVI GTATCCnnnnn_n'
Cuts at: 2394 3155 4496 2394
Size: 761 1341 2394
Fragments arranged by size:
2394 1341 761
BclI T'GATC_A
Cuts at: 2016 2016
Size: 4496
BglII A'GATC_T
Cuts at: 2011 4048 2011
Size: 2037 2459
BlpI GC'TnA_GC
Cuts at: 1608 1608
Size: 4496
Bme1580I G_kGCm'C
Cuts at: 2121 2214 3270 2121
Size: 93 1056 3347
Fragments arranged by size:
3347 1056 93
BmgBI CAC'GTC
Cuts at: 484 484
Size: 4496
BmrI ACTGGGnnnn_n'
Cuts at: 722 1142 1474 1708 1789 2124 4268 722
Size: 420 332 234 81 335 2144 950
Fragments arranged by size:
2144 950 420 335 332 234 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 416 614 782 866 1202 1286 1370 1454
Size: 244 198 168 84 336 84 84 84
Cuts at: 1454 172
Size: 3214
Fragments arranged by size:
3214 336 244 198 168 84 84 84
84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 570 1672 2551 3043 3341 570
Size: 1102 879 492 298 1725
Fragments arranged by size:
1725 1102 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4200 4200
Size: 4496
BsaAI yAC'GTr
Cuts at: 529 577 1849 529
Size: 48 1272 3176
Fragments arranged by size:
3176 1272 48
BsaBI GATnn'nnATC
Cuts at: 6 2010 4047 6
Size: 2004 2037 455
Fragments arranged by size:
2037 2004 455
BsaHI Gr'CG_yC
Cuts at: 1927 2175 2877 4373 1927
Size: 248 702 1496 2050
Fragments arranged by size:
2050 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1012 1685 2206 2884 3158 3305 3716 1012
Size: 673 521 678 274 147 411 1792
Fragments arranged by size:
1792 678 673 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 169
Size: 4496
BseYI C'CCAG_C
Cuts at: 1921 2679 3256 3752 1921
Size: 758 577 496 2665
Fragments arranged by size:
2665 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2038 2084 3292 104
Size: 1934 46 1208 1308
Fragments arranged by size:
1934 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1510 2288 2478 3270 1510
Size: 778 190 792 2736
Fragments arranged by size:
2736 792 778 190
BsmI GAATG_Cn'
Cuts at: 650 784 868 1045 650
Size: 134 84 177 4101
Fragments arranged by size:
4101 177 134 84
Bsp1286I G_dGCh'C
Cuts at: 1510 2121 2214 2288 2478 2540 3270 1510
Size: 611 93 74 190 62 730 2736
Fragments arranged by size:
2736 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3716 3716
Size: 4496
BspHI T'CATG_A
Cuts at: 3672 3672
Size: 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1546 1760 2062 2443 1546
Size: 214 302 381 3599
Fragments arranged by size:
3599 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2788 2842 4326 4491 50
Size: 2738 54 1484 165 55
Fragments arranged by size:
2738 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 793 1107 1213 1381 2408 793
Size: 314 106 168 1027 2881
Fragments arranged by size:
2881 1027 314 168 106
BsrFI r'CCGG_y
Cuts at: 2494 2675 2884 2494
Size: 181 209 4106
Fragments arranged by size:
4106 209 181
BsrGI T'GTAC_A
Cuts at: 1285 1285
Size: 4496
BssSI C'ACGA_G
Cuts at: 997 2767 3125 997
Size: 1770 358 2368
Fragments arranged by size:
2368 1770 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1873 1873
Size: 4496
BstBI TT'CG_AA
Cuts at: 654 906 990 1158 1242 2857 654
Size: 252 84 168 84 1615 2293
Fragments arranged by size:
2293 1615 252 168 84 84
BstYI r'GATC_y
Cuts at: 1 171 1521 1988 2011 2346 2592 3604 3690
Size: 170 1350 467 23 335 246 1012 86
Cuts at: 3690 3702 4048 1
Size: 12 346 449
Fragments arranged by size:
1350 1012 467 449 346 335 246 170
86 23 12
BstZ17I GTA'TAC
Cuts at: 742 742
Size: 4496
BtgI C'CryG_G
Cuts at: 107 107
Size: 4496
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 544 2446 2627 2790 544
Size: 1902 181 163 2250
Fragments arranged by size:
2250 1902 181 163
BtsI GCAGTG_nn'
Cuts at: 423 877 1739 423
Size: 454 862 3180
Fragments arranged by size:
3180 862 454
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 771 806 855 890 771
Size: 35 49 35 4377
Fragments arranged by size:
4377 49 35 35
DraI TTT'AAA
Cuts at: 1495 1495
Size: 4496
DrdI GACnn_nn'nnGTC
Cuts at: 2202 3060 2202
Size: 858 3638
EaeI y'GGCC_r
Cuts at: 2081 2255 2646 2673 4018 2081
Size: 174 391 27 1345 2559
Fragments arranged by size:
2559 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2081 2081
Size: 4496
EarI CTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2704 3014 3160 4306 2704
Size: 310 146 1146 2894
Fragments arranged by size:
2894 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1508 1508
Size: 4496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 201 339 416 614 782 786 866 1202
Size: 29 138 77 198 168 4 80 336
Cuts at: 1202 1206 1286 1290 1370 1374 1454 2321 2753
Size: 4 80 4 80 4 80 867 432
Cuts at: 2753 3500 3768 172
Size: 747 268 900
Fragments arranged by size:
900 867 747 432 336 268 198 168
138 80 80 80 80 77 29 4
4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 442 1172 1635 442
Size: 730 463 3303
Fragments arranged by size:
3303 730 463
EcoRI G'AATT_C
Cuts at: 1256 1500 1256
Size: 244 4252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4496
FspI TGC'GCA
Cuts at: 2277 2277
Size: 4496
HaeII r_GCGC'y
Cuts at: 1930 2178 3200 4179 1930
Size: 248 1022 979 2247
Fragments arranged by size:
2247 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 543 575 2338 2368 2370 2400 543
Size: 32 1763 30 2 30 2639
Fragments arranged by size:
2639 1763 32 30 30 2
HincII GTy'rAC
Cuts at: 668 1535 668
Size: 867 3629
HindIII A'AGCT_T
Cuts at: 881 881
Size: 4496
HpaI GTT'AAC
Cuts at: 668 668
Size: 4496
KasI G'GCGC_C
Cuts at: 1926 2174 1926
Size: 248 4248
KpnI G_GTAC'C
Cuts at: 1516 1516
Size: 4496
MluI A'CGCG_T
Cuts at: 3929 3929
Size: 4496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3167 3351 3167
Size: 184 4312
MscI TGG'CCA
Cuts at: 2257 2257
Size: 4496
MslI CAynn'nnrTG
Cuts at: 2485 2612 2485
Size: 127 4369
MspA1I CmG'CkG
Cuts at: 1607 1921 2281 3294 3539 1607
Size: 314 360 1013 245 2564
Fragments arranged by size:
2564 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2677 2677
Size: 4496
NarI GG'CG_CC
Cuts at: 1927 2175 1927
Size: 248 4248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4496
NgoMIV G'CCGG_C
Cuts at: 2675 2675
Size: 4496
NspI r_CATG'y
Cuts at: 666 1549 2049 2956 666
Size: 883 500 907 2206
Fragments arranged by size:
2206 907 883 500
PciI A'CATG_T
Cuts at: 662 2952 662
Size: 2290 2206
PfoI T'CCnGG_A
Cuts at: 393 591 393
Size: 198 4298
PmeI GTTT'AAAC
Cuts at: 1495 1495
Size: 4496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3635 3667 3635
Size: 32 4464
PpuMI rG'GwC_Cy
Cuts at: 442 1172 442
Size: 730 3766
PsiI TTA'TAA
Cuts at: 1112 1112
Size: 4496
PstI C_TGCA'G
Cuts at: 1543 2228 1543
Size: 685 3811
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4496
PvuII CAG'CTG
Cuts at: 1921 2281 1921
Size: 360 4136
RsrII CG'GwC_CG
Cuts at: 2691 2691
Size: 4496
SacI G_AGCT'C
Cuts at: 1510 1510
Size: 4496
SalI G'TCGA_C
Cuts at: 1533 1533
Size: 4496
SapI GCTCTTCn'nnn_
Cuts at: 2519 2729 2519
Size: 210 4286
SbfI CC_TGCA'GG
Cuts at: 1543 1543
Size: 4496
ScaI AGT'ACT
Cuts at: 4013 4013
Size: 4496
SfcI C'TryA_G
Cuts at: 33 192 233 1539 2224 3217 3408 33
Size: 159 41 1306 685 993 191 1121
Fragments arranged by size:
1306 1121 993 685 191 159 41
SfoI GGC'GCC
Cuts at: 1928 2176 1928
Size: 248 4248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4496
SmaI CCC'GGG
Cuts at: 1518 1518
Size: 4496
SmlI C'TyrA_G
Cuts at: 549 1651 2566 3058 3320 549
Size: 1102 915 492 262 1725
Fragments arranged by size:
1725 1102 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4496
SphI G_CATG'C
Cuts at: 1549 2049 1549
Size: 500 3996
StyI C'CwwG_G
Cuts at: 107 1630 1984 107
Size: 1523 354 2619
Fragments arranged by size:
2619 1523 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2079 2212 2671 2880 2079
Size: 133 459 209 3695
Fragments arranged by size:
3695 459 209 133
TatI w'GTAC_w
Cuts at: 899 1285 1413 4011 899
Size: 386 128 2598 1384
Fragments arranged by size:
2598 1384 386 128
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 4464
Tth111I GACn'n_nGTC
Cuts at: 2293 2293
Size: 4496
XbaI T'CTAG_A
Cuts at: 1527 1527
Size: 4496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 367 1991 114
Size: 253 1624 2619
Fragments arranged by size:
2619 1624 253
XmaI C'CCGG_G
Cuts at: 1516 1516
Size: 4496
XmnI GAAnn'nnTTC
Cuts at: 799 883 1219 1256 1303 1387 1424 799
Size: 84 336 37 47 84 37 3871
Fragments arranged by size:
3871 336 84 84 47 37 37
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BssHII BstEII
BstXI Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI