Restriction map for
pF1KB5977
(Circular) (Six-base) MAPSORT of: pF1KB5977.seq Check: 6253 from: 1 to: 4670
pF1KB5977 4670 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AccI GT'mk_AC
Cuts at: 1708 1708
Size: 4670
Acc65I G'GTAC_C
Cuts at: 1686 1686
Size: 4670
AclI AA'CG_TT
Cuts at: 4510 4510
Size: 4670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1418 1667 2495 2927 3674 3942 1418
Size: 249 828 432 747 268 2146
Fragments arranged by size:
2146 828 747 432 268 249
AflIII A'CryG_T
Cuts at: 705 2022 3126 4103 705
Size: 1317 1104 977 1272
Fragments arranged by size:
1317 1272 1104 977
AgeI A'CCGG_T
Cuts at: 3058 3058
Size: 4670
AlwNI CAG_nnn'CTG
Cuts at: 933 1975 3542 933
Size: 1042 1567 2061
Fragments arranged by size:
2061 1567 1042
ApaLI G'TGCA_C
Cuts at: 3440 3440
Size: 4670
ApoI r'AATT_y
Cuts at: 637 1674 4023 4279 637
Size: 1037 2349 256 1028
Fragments arranged by size:
2349 1037 1028 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4670
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
AvaI C'yCGr_G
Cuts at: 1690 1690
Size: 4670
BamHI G'GATC_C
Cuts at: 870 1695 870
Size: 825 3845
BanI G'GyrC_C
Cuts at: 1686 2100 2348 2383 1686
Size: 414 248 35 3973
Fragments arranged by size:
3973 414 248 35
BanII G_rGCy'C
Cuts at: 1684 2714 1684
Size: 1030 3640
BbeI G_GCGC'C
Cuts at: 2104 2352 2104
Size: 248 4422
BbsI GAAGACnn'nnnn_
Cuts at: 238 785 238
Size: 547 4123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1325 1359 1325
Size: 34 4636
BciVI GTATCCnnnnn_n'
Cuts at: 2568 3329 4670 2568
Size: 761 1341 2568
Fragments arranged by size:
2568 1341 761
BclI T'GATC_A
Cuts at: 2190 2190
Size: 4670
BfrBI ATG'CAT
Cuts at: 1018 1018
Size: 4670
BglI GCCn_nnn'nGGC
Cuts at: 112 586 112
Size: 474 4196
BglII A'GATC_T
Cuts at: 321 2185 4222 321
Size: 1864 2037 769
Fragments arranged by size:
2037 1864 769
BlpI GC'TnA_GC
Cuts at: 1782 1782
Size: 4670
Bme1580I G_kGCm'C
Cuts at: 2295 2388 3444 2295
Size: 93 1056 3521
Fragments arranged by size:
3521 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1638 1882 1963 2298 4442 1638
Size: 244 81 335 2144 1866
Fragments arranged by size:
2144 1866 335 244 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 143 836 3037 143
Size: 693 2201 1776
Fragments arranged by size:
2201 1776 693
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 215 716 190
Size: 25 501 4144
Fragments arranged by size:
4144 501 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1846 2725 3217 3515 1846
Size: 879 492 298 3001
Fragments arranged by size:
3001 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4374 4374
Size: 4670
BsaAI yAC'GTr
Cuts at: 2023 2023
Size: 4670
BsaBI GATnn'nnATC
Cuts at: 6 2184 4221 6
Size: 2178 2037 455
Fragments arranged by size:
2178 2037 455
BsaHI Gr'CG_yC
Cuts at: 2101 2349 3051 4547 2101
Size: 248 702 1496 2224
Fragments arranged by size:
2224 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1859 2380 3058 3332 3479 3890 1859
Size: 521 678 274 147 411 2639
Fragments arranged by size:
2639 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 119 1234 119
Size: 1115 3555
BseYI C'CCAG_C
Cuts at: 135 2095 2853 3430 3926 135
Size: 1960 758 577 496 879
Fragments arranged by size:
1960 879 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 116 2212 2258 3466 104
Size: 12 2096 46 1208 1308
Fragments arranged by size:
2096 1308 1208 46 12
BsiHKAI G_wGCw'C
Cuts at: 1684 2462 2652 3444 1684
Size: 778 190 792 2910
Fragments arranged by size:
2910 792 778 190
BsmI GAATG_Cn'
Cuts at: 582 1636 582
Size: 1054 3616
Bsp1286I G_dGCh'C
Cuts at: 1684 2295 2388 2462 2652 2714 3444 1684
Size: 611 93 74 190 62 730 2910
Fragments arranged by size:
2910 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3890 3890
Size: 4670
BspHI T'CATG_A
Cuts at: 3846 3846
Size: 4670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1720 1934 2236 2617 1720
Size: 214 302 381 3773
Fragments arranged by size:
3773 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2962 3016 4500 4665 50
Size: 2912 54 1484 165 55
Fragments arranged by size:
2912 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 431 1658 2582 431
Size: 1227 924 2519
Fragments arranged by size:
2519 1227 924
BsrFI r'CCGG_y
Cuts at: 2668 2849 3058 2668
Size: 181 209 4280
Fragments arranged by size:
4280 209 181
BssSI C'ACGA_G
Cuts at: 2941 3299 2941
Size: 358 4312
BstAPI GCAn_nnn'nTGC
Cuts at: 2047 2047
Size: 4670
BstBI TT'CG_AA
Cuts at: 1521 3031 1521
Size: 1510 3160
BstYI r'GATC_y
Cuts at: 1 165 321 484 549 870 1695 2162 2185
Size: 164 156 163 65 321 825 467 23
Cuts at: 2185 2520 2766 3778 3864 3876 4222 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 825 467 449 346 335 321 246
164 163 156 86 65 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4670
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2620 2801 2964 2620
Size: 181 163 4326
Fragments arranged by size:
4326 181 163
BtsI GCAGTG_nn'
Cuts at: 1319 1913 1319
Size: 594 4076
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 141 176 279 314 780 815 141
Size: 35 103 35 466 35 3996
Fragments arranged by size:
3996 466 103 35 35 35
DraI TTT'AAA
Cuts at: 1566 1669 1566
Size: 103 4567
DrdI GACnn_nn'nnGTC
Cuts at: 2376 3234 2376
Size: 858 3812
EaeI y'GGCC_r
Cuts at: 113 2255 2429 2820 2847 4192 113
Size: 2142 174 391 27 1345 591
Fragments arranged by size:
2142 1345 591 391 174 27
EagI C'GGCC_G
Cuts at: 113 2255 113
Size: 2142 2528
EarI CTCTTCn'nnn_
Cuts at: 488 2693 2903 488
Size: 2205 210 2255
Fragments arranged by size:
2255 2205 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2878 3188 3334 4480 2878
Size: 310 146 1146 3068
Fragments arranged by size:
3068 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1682 1682
Size: 4670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 215 716 1418 1667 2495 2927 3674 3942
Size: 25 501 702 249 828 432 747 268
Cuts at: 3942 190
Size: 918
Fragments arranged by size:
918 828 747 702 501 432 268 249
25
EcoO109I rG'GnC_Cy
Cuts at: 183 1809 183
Size: 1626 3044
EcoRI G'AATT_C
Cuts at: 1674 1674
Size: 4670
EcoRV GAT'ATC
Cuts at: 1508 1508
Size: 4670
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2689 2689
Size: 4670
FspI TGC'GCA
Cuts at: 2451 2451
Size: 4670
HaeII r_GCGC'y
Cuts at: 2104 2352 3374 4353 2104
Size: 248 1022 979 2421
Fragments arranged by size:
2421 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 772 804 2512 2542 2544 2574 772
Size: 32 1708 30 2 30 2868
Fragments arranged by size:
2868 1708 32 30 30 2
HincII GTy'rAC
Cuts at: 984 1709 984
Size: 725 3945
HindIII A'AGCT_T
Cuts at: 1250 1250
Size: 4670
KasI G'GCGC_C
Cuts at: 2100 2348 2100
Size: 248 4422
KpnI G_GTAC'C
Cuts at: 1690 1690
Size: 4670
MluI A'CGCG_T
Cuts at: 4103 4103
Size: 4670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 880 3341 3525 880
Size: 2461 184 2025
Fragments arranged by size:
2461 2025 184
MscI TGG'CCA
Cuts at: 2431 2431
Size: 4670
MslI CAynn'nnrTG
Cuts at: 1082 2659 2786 1082
Size: 1577 127 2966
Fragments arranged by size:
2966 1577 127
MspA1I CmG'CkG
Cuts at: 135 1132 1617 1781 2095 2455 3468 3713 135
Size: 997 485 164 314 360 1013 245 1092
Fragments arranged by size:
1092 1013 997 485 360 314 245 164
NaeI GCC'GGC
Cuts at: 2851 2851
Size: 4670
NarI GG'CG_CC
Cuts at: 2101 2349 2101
Size: 248 4422
NcoI C'CATG_G
Cuts at: 107 107
Size: 4670
NgoMIV G'CCGG_C
Cuts at: 2849 2849
Size: 4670
NsiI A_TGCA'T
Cuts at: 1020 1020
Size: 4670
NspI r_CATG'y
Cuts at: 709 1723 2223 3130 709
Size: 1014 500 907 2249
Fragments arranged by size:
2249 1014 907 500
PciI A'CATG_T
Cuts at: 705 3126 705
Size: 2421 2249
PmeI GTTT'AAAC
Cuts at: 1669 1669
Size: 4670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 172 204 3809 3841 172
Size: 32 3605 32 1001
Fragments arranged by size:
3605 1001 32 32
PpuMI rG'GwC_Cy
Cuts at: 183 183
Size: 4670
PshAI GACnn'nnGTC
Cuts at: 760 760
Size: 4670
PstI C_TGCA'G
Cuts at: 255 405 1314 1717 2402 255
Size: 150 909 403 685 2523
Fragments arranged by size:
2523 909 685 403 150
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4670
PvuII CAG'CTG
Cuts at: 1132 1617 2095 2455 1132
Size: 485 478 360 3347
Fragments arranged by size:
3347 485 478 360
RsrII CG'GwC_CG
Cuts at: 2865 2865
Size: 4670
SacI G_AGCT'C
Cuts at: 1684 1684
Size: 4670
SalI G'TCGA_C
Cuts at: 1707 1707
Size: 4670
SapI GCTCTTCn'nnn_
Cuts at: 2693 2903 2693
Size: 210 4460
SbfI CC_TGCA'GG
Cuts at: 1717 1717
Size: 4670
ScaI AGT'ACT
Cuts at: 1585 4187 1585
Size: 2602 2068
SfcI C'TryA_G
Cuts at: 33 251 401 865 1310 1713 2398 3391 3582
Size: 218 150 464 445 403 685 993 191
Cuts at: 3582 33
Size: 1121
Fragments arranged by size:
1121 993 685 464 445 403 218 191
150
SfoI GGC'GCC
Cuts at: 2102 2350 2102
Size: 248 4422
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
SmaI CCC'GGG
Cuts at: 1692 1692
Size: 4670
SmlI C'TyrA_G
Cuts at: 1825 2740 3232 3494 1825
Size: 915 492 262 3001
Fragments arranged by size:
3001 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1596 67
Size: 1529 3141
SphI G_CATG'C
Cuts at: 1723 2223 1723
Size: 500 4170
SspI AAT'ATT
Cuts at: 700 950 700
Size: 250 4420
StyI C'CwwG_G
Cuts at: 107 758 1094 1804 2158 107
Size: 651 336 710 354 2619
Fragments arranged by size:
2619 710 651 354 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2253 2386 2845 3054 2253
Size: 133 459 209 3869
Fragments arranged by size:
3869 459 209 133
TatI w'GTAC_w
Cuts at: 1583 4185 1583
Size: 2602 2068
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4187 4219 4187
Size: 32 4638
Tth111I GACn'n_nGTC
Cuts at: 979 2467 979
Size: 1488 3182
XbaI T'CTAG_A
Cuts at: 1189 1701 1189
Size: 512 4158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 765 2165 765
Size: 1400 3270
XmaI C'CCGG_G
Cuts at: 1690 1690
Size: 4670
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI FseI FspAI HpaI
MfeI NdeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI