Restriction map for
pF1KB5974
(Circular) (Six-base) MAPSORT of: pF1KB5974.seq Check: 9784 from: 1 to: 4793
pF1KB5974 4793 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 1288 1831 1288
Size: 543 4250
Acc65I G'GTAC_C
Cuts at: 1809 1809
Size: 4793
AclI AA'CG_TT
Cuts at: 4633 4633
Size: 4793
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 395 411 1101 1202 2618 3050 3797 4065
Size: 226 16 690 101 1416 432 747 268
Cuts at: 4065 169
Size: 897
Fragments arranged by size:
1416 897 747 690 432 268 226 101
16
AflIII A'CryG_T
Cuts at: 2145 3249 4226 2145
Size: 1104 977 2712
Fragments arranged by size:
2712 1104 977
AgeI A'CCGG_T
Cuts at: 3181 3181
Size: 4793
AlwNI CAG_nnn'CTG
Cuts at: 1705 2098 3665 1705
Size: 393 1567 2833
Fragments arranged by size:
2833 1567 393
ApaLI G'TGCA_C
Cuts at: 1736 3563 1736
Size: 1827 2966
ApoI r'AATT_y
Cuts at: 343 807 1797 4146 4402 343
Size: 464 990 2349 256 734
Fragments arranged by size:
2349 990 734 464 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4793
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4793
AvaI C'yCGr_G
Cuts at: 1813 1813
Size: 4793
BamHI G'GATC_C
Cuts at: 432 1818 432
Size: 1386 3407
BanI G'GyrC_C
Cuts at: 1809 2223 2471 2506 1809
Size: 414 248 35 4096
Fragments arranged by size:
4096 414 248 35
BanII G_rGCy'C
Cuts at: 1807 2837 1807
Size: 1030 3763
BbeI G_GCGC'C
Cuts at: 2227 2475 2227
Size: 248 4545
BbsI GAAGACnn'nnnn_
Cuts at: 1543 1543
Size: 4793
BciVI GTATCCnnnnn_n'
Cuts at: 2691 3452 4793 2691
Size: 761 1341 2691
Fragments arranged by size:
2691 1341 761
BclI T'GATC_A
Cuts at: 1137 2313 1137
Size: 1176 3617
BfrBI ATG'CAT
Cuts at: 529 841 529
Size: 312 4481
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4793
BglII A'GATC_T
Cuts at: 406 2308 4345 406
Size: 1902 2037 854
Fragments arranged by size:
2037 1902 854
BlpI GC'TnA_GC
Cuts at: 1905 1905
Size: 4793
Bme1580I G_kGCm'C
Cuts at: 637 1740 2418 2511 3567 637
Size: 1103 678 93 1056 1863
Fragments arranged by size:
1863 1103 1056 678 93
BmrI ACTGGGnnnn_n'
Cuts at: 646 1193 2005 2086 2421 4565 646
Size: 547 812 81 335 2144 874
Fragments arranged by size:
2144 874 812 547 335 81
BmtI G_CTAG'C
Cuts at: 1310 1310
Size: 4793
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3160 3160
Size: 4793
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 966 1260 1727 722
Size: 244 294 467 3788
Fragments arranged by size:
3788 467 294 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 1969 2848 3340 3638 653
Size: 1316 879 492 298 1808
Fragments arranged by size:
1808 1316 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4497 4497
Size: 4793
BsaAI yAC'GTr
Cuts at: 2146 2146
Size: 4793
BsaBI GATnn'nnATC
Cuts at: 6 2307 4344 6
Size: 2301 2037 455
Fragments arranged by size:
2301 2037 455
BsaHI Gr'CG_yC
Cuts at: 2224 2472 3174 4670 2224
Size: 248 702 1496 2347
Fragments arranged by size:
2347 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1982 2503 3181 3455 3602 4013 1982
Size: 521 678 274 147 411 2762
Fragments arranged by size:
2762 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1524 1554 1524
Size: 30 4763
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 734 1490 151
Size: 583 756 3454
Fragments arranged by size:
3454 756 583
BseYI C'CCAG_C
Cuts at: 2218 2976 3553 4049 2218
Size: 758 577 496 2962
Fragments arranged by size:
2962 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 604 1331 593
Size: 11 727 4055
Fragments arranged by size:
4055 727 11
BsiEI CG_ry'CG
Cuts at: 104 239 2335 2381 3589 104
Size: 135 2096 46 1208 1308
Fragments arranged by size:
2096 1308 1208 135 46
BsiHKAI G_wGCw'C
Cuts at: 1740 1807 2585 2775 3567 1740
Size: 67 778 190 792 2966
Fragments arranged by size:
2966 792 778 190 67
Bsp1286I G_dGCh'C
Cuts at: 637 1740 1807 2418 2511 2585 2775 2837 3567
Size: 1103 67 611 93 74 190 62 730
Cuts at: 3567 637
Size: 1863
Fragments arranged by size:
1863 1103 730 611 190 93 74 67
62
BspEI T'CCGG_A
Cuts at: 4013 4013
Size: 4793
BspHI T'CATG_A
Cuts at: 3969 3969
Size: 4793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1843 2057 2359 2740 1843
Size: 214 302 381 3896
Fragments arranged by size:
3896 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3085 3139 4623 4788 50
Size: 3035 54 1484 165 55
Fragments arranged by size:
3035 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1320 2705 1320
Size: 1385 3408
BsrFI r'CCGG_y
Cuts at: 169 2791 2972 3181 169
Size: 2622 181 209 1781
Fragments arranged by size:
2622 1781 209 181
BssSI C'ACGA_G
Cuts at: 3064 3422 3064
Size: 358 4435
BstAPI GCAn_nnn'nTGC
Cuts at: 826 1096 2170 826
Size: 270 1074 3449
Fragments arranged by size:
3449 1074 270
BstBI TT'CG_AA
Cuts at: 3154 3154
Size: 4793
BstXI CCAn_nnnn'nTGG
Cuts at: 513 513
Size: 4793
BstYI r'GATC_y
Cuts at: 1 406 432 1818 2285 2308 2643 2889 3901
Size: 405 26 1386 467 23 335 246 1012
Cuts at: 3901 3987 3999 4345 1
Size: 86 12 346 449
Fragments arranged by size:
1386 1012 467 449 405 346 335 246
86 26 23 12
BstZ17I GTA'TAC
Cuts at: 1289 1289
Size: 4793
Bsu36I CC'TnA_GG
Cuts at: 572 572
Size: 4793
BtgI C'CryG_G
Cuts at: 107 1520 107
Size: 1413 3380
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2743 2924 3087 2743
Size: 181 163 4449
Fragments arranged by size:
4449 181 163
BtsI GCAGTG_nn'
Cuts at: 616 1499 2036 616
Size: 883 537 3373
Fragments arranged by size:
3373 883 537
DraI TTT'AAA
Cuts at: 1792 1792
Size: 4793
DraIII CAC_nnn'GTG
Cuts at: 300 300
Size: 4793
DrdI GACnn_nn'nnGTC
Cuts at: 2499 3357 2499
Size: 858 3935
EaeI y'GGCC_r
Cuts at: 113 2378 2552 2943 2970 4315 113
Size: 2265 174 391 27 1345 591
Fragments arranged by size:
2265 1345 591 391 174 27
EagI C'GGCC_G
Cuts at: 2378 2378
Size: 4793
EarI CTCTTCn'nnn_
Cuts at: 2816 3026 2816
Size: 210 4583
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3001 3311 3457 4603 3001
Size: 310 146 1146 3191
Fragments arranged by size:
3191 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1805 1805
Size: 4793
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 395 411 722 966 1101 1202 1260 1727
Size: 226 16 311 244 135 101 58 467
Cuts at: 1727 2618 3050 3797 4065 169
Size: 891 432 747 268 897
Fragments arranged by size:
897 891 747 467 432 311 268 244
226 135 101 58 16
EcoO109I rG'GnC_Cy
Cuts at: 542 1932 542
Size: 1390 3403
EcoRI G'AATT_C
Cuts at: 343 807 1797 343
Size: 464 990 3339
Fragments arranged by size:
3339 990 464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1232 2812 1232
Size: 1580 3213
FspI TGC'GCA
Cuts at: 2574 2574
Size: 4793
HaeII r_GCGC'y
Cuts at: 2227 2475 3497 4476 2227
Size: 248 1022 979 2544
Fragments arranged by size:
2544 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 391 423 473 505 703 735 2635 2665 2667
Size: 32 50 32 198 32 1900 30 2
Cuts at: 2667 2697 391
Size: 30 2487
Fragments arranged by size:
2487 1900 198 50 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 228 752 1832 228
Size: 524 1080 3189
Fragments arranged by size:
3189 1080 524
HpaI GTT'AAC
Cuts at: 228 228
Size: 4793
KasI G'GCGC_C
Cuts at: 2223 2471 2223
Size: 248 4545
KpnI G_GTAC'C
Cuts at: 1813 1813
Size: 4793
MluI A'CGCG_T
Cuts at: 4226 4226
Size: 4793
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1780 3464 3648 1780
Size: 1684 184 2925
Fragments arranged by size:
2925 1684 184
MscI TGG'CCA
Cuts at: 2554 2554
Size: 4793
MslI CAynn'nnrTG
Cuts at: 362 1513 2782 2909 362
Size: 1151 1269 127 2246
Fragments arranged by size:
2246 1269 1151 127
MspA1I CmG'CkG
Cuts at: 1036 1486 1702 1904 2218 2578 3591 3836 1036
Size: 450 216 202 314 360 1013 245 1993
Fragments arranged by size:
1993 1013 450 360 314 245 216 202
NaeI GCC'GGC
Cuts at: 2974 2974
Size: 4793
NarI GG'CG_CC
Cuts at: 2224 2472 2224
Size: 248 4545
NcoI C'CATG_G
Cuts at: 107 107
Size: 4793
NgoMIV G'CCGG_C
Cuts at: 2972 2972
Size: 4793
NheI G'CTAG_C
Cuts at: 1306 1306
Size: 4793
NsiI A_TGCA'T
Cuts at: 531 843 531
Size: 312 4481
NspI r_CATG'y
Cuts at: 1518 1846 2346 3253 1518
Size: 328 500 907 3058
Fragments arranged by size:
3058 907 500 328
PciI A'CATG_T
Cuts at: 3249 3249
Size: 4793
PmeI GTTT'AAAC
Cuts at: 1792 1792
Size: 4793
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 3932
Size: 32 4761
PpuMI rG'GwC_Cy
Cuts at: 542 542
Size: 4793
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 990 1022 990
Size: 32 4761
PstI C_TGCA'G
Cuts at: 1080 1269 1840 2525 1080
Size: 189 571 685 3348
Fragments arranged by size:
3348 685 571 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4793
PvuII CAG'CTG
Cuts at: 1036 1702 2218 2578 1036
Size: 666 516 360 3251
Fragments arranged by size:
3251 666 516 360
RsrII CG'GwC_CG
Cuts at: 2988 2988
Size: 4793
SacI G_AGCT'C
Cuts at: 1807 1807
Size: 4793
SalI G'TCGA_C
Cuts at: 1830 1830
Size: 4793
SapI GCTCTTCn'nnn_
Cuts at: 2816 3026 2816
Size: 210 4583
SbfI CC_TGCA'GG
Cuts at: 1840 1840
Size: 4793
ScaI AGT'ACT
Cuts at: 4310 4310
Size: 4793
SfcI C'TryA_G
Cuts at: 33 758 917 1076 1265 1545 1836 2521 3514
Size: 725 159 159 189 280 291 685 993
Cuts at: 3514 3705 33
Size: 191 1121
Fragments arranged by size:
1121 993 725 685 291 280 191 189
159 159
SfoI GGC'GCC
Cuts at: 2225 2473 2225
Size: 248 4545
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4793
SmaI CCC'GGG
Cuts at: 1815 1815
Size: 4793
SmlI C'TyrA_G
Cuts at: 668 1948 2863 3355 3617 668
Size: 1280 915 492 262 1844
Fragments arranged by size:
1844 1280 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4793
SphI G_CATG'C
Cuts at: 1518 1846 2346 1518
Size: 328 500 3965
Fragments arranged by size:
3965 500 328
StyI C'CwwG_G
Cuts at: 107 1927 2281 107
Size: 1820 354 2619
Fragments arranged by size:
2619 1820 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2376 2509 2968 3177 2376
Size: 133 459 209 3992
Fragments arranged by size:
3992 459 209 133
TatI w'GTAC_w
Cuts at: 4308 4308
Size: 4793
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1524 1556 4310 4342 1524
Size: 32 2754 32 1975
Fragments arranged by size:
2754 1975 32 32
Tth111I GACn'n_nGTC
Cuts at: 2590 2590
Size: 4793
XbaI T'CTAG_A
Cuts at: 1824 1824
Size: 4793
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 919 2288 919
Size: 1369 3424
XmaI C'CCGG_G
Cuts at: 1813 1813
Size: 4793
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PciI PmeI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BmgBI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII MfeI NdeI NotI
NruI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI