Restriction map for
pF1KB5972
(Circular) (Six-base) MAPSORT of: pF1KB5972.seq Check: 9404 from: 1 to: 4796
pF1KB5972 4796 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 1834 1834
Size: 4796
Acc65I G'GTAC_C
Cuts at: 1812 1812
Size: 4796
AclI AA'CG_TT
Cuts at: 4636 4636
Size: 4796
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 467 1098 2621 3053 3800 4068 467
Size: 631 1523 432 747 268 1195
Fragments arranged by size:
1523 1195 747 631 432 268
AflIII A'CryG_T
Cuts at: 2148 3252 4229 2148
Size: 1104 977 2715
Fragments arranged by size:
2715 1104 977
AgeI A'CCGG_T
Cuts at: 3184 3184
Size: 4796
AleI CACnn'nnGTG
Cuts at: 1325 1409 1325
Size: 84 4712
AlwNI CAG_nnn'CTG
Cuts at: 180 488 2101 3668 180
Size: 308 1613 1567 1308
Fragments arranged by size:
1613 1567 1308 308
ApaLI G'TGCA_C
Cuts at: 3566 3566
Size: 4796
ApoI r'AATT_y
Cuts at: 322 579 1038 1064 1444 1800 4149 4405 322
Size: 257 459 26 380 356 2349 256 713
Fragments arranged by size:
2349 713 459 380 356 257 256 26
AseI AT'TA_AT
Cuts at: 20 349 353 20
Size: 329 4 4463
Fragments arranged by size:
4463 329 4
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
AvaI C'yCGr_G
Cuts at: 1816 1816
Size: 4796
BamHI G'GATC_C
Cuts at: 1821 1821
Size: 4796
BanI G'GyrC_C
Cuts at: 1812 2226 2474 2509 1812
Size: 414 248 35 4099
Fragments arranged by size:
4099 414 248 35
BanII G_rGCy'C
Cuts at: 1810 2840 1810
Size: 1030 3766
BbeI G_GCGC'C
Cuts at: 2230 2478 2230
Size: 248 4548
BbsI GAAGACnn'nnnn_
Cuts at: 506 1030 1491 506
Size: 524 461 3811
Fragments arranged by size:
3811 524 461
BciVI GTATCCnnnnn_n'
Cuts at: 2694 3455 4796 2694
Size: 761 1341 2694
Fragments arranged by size:
2694 1341 761
BclI T'GATC_A
Cuts at: 110 2316 110
Size: 2206 2590
BfrBI ATG'CAT
Cuts at: 1267 1267
Size: 4796
BglII A'GATC_T
Cuts at: 2311 4348 2311
Size: 2037 2759
BlpI GC'TnA_GC
Cuts at: 1908 1908
Size: 4796
Bme1580I G_kGCm'C
Cuts at: 2421 2514 3570 2421
Size: 93 1056 3647
Fragments arranged by size:
3647 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2008 2089 2424 4568 2008
Size: 81 335 2144 2236
Fragments arranged by size:
2236 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3163 3163
Size: 4796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 241
Size: 4796
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 265 1972 2851 3343 3641 146
Size: 119 1707 879 492 298 1301
Fragments arranged by size:
1707 1301 879 492 298 119
BsaI GGTCTCn'nnnn_
Cuts at: 1074 4500 1074
Size: 3426 1370
BsaAI yAC'GTr
Cuts at: 2149 2149
Size: 4796
BsaBI GATnn'nnATC
Cuts at: 6 2310 4347 6
Size: 2304 2037 455
Fragments arranged by size:
2304 2037 455
BsaHI Gr'CG_yC
Cuts at: 2227 2475 3177 4673 2227
Size: 248 702 1496 2350
Fragments arranged by size:
2350 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1462 1985 2506 3184 3458 3605 4016 1462
Size: 523 521 678 274 147 411 2242
Fragments arranged by size:
2242 678 523 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 313 181
Size: 132 4664
BseYI C'CCAG_C
Cuts at: 2221 2979 3556 4052 2221
Size: 758 577 496 2965
Fragments arranged by size:
2965 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2338 2384 3592 104
Size: 2234 46 1208 1308
Fragments arranged by size:
2234 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1810 2588 2778 3570 1810
Size: 778 190 792 3036
Fragments arranged by size:
3036 792 778 190
BsmI GAATG_Cn'
Cuts at: 257 1264 257
Size: 1007 3789
Bsp1286I G_dGCh'C
Cuts at: 1810 2421 2514 2588 2778 2840 3570 1810
Size: 611 93 74 190 62 730 3036
Fragments arranged by size:
3036 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4016 4016
Size: 4796
BspHI T'CATG_A
Cuts at: 927 1011 1095 1179 3972 927
Size: 84 84 84 2793 1751
Fragments arranged by size:
2793 1751 84 84 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 634 1846 2060 2362 2743 634
Size: 1212 214 302 381 2687
Fragments arranged by size:
2687 1212 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3088 3142 4626 4791 50
Size: 3038 54 1484 165 55
Fragments arranged by size:
3038 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1637 2708 1637
Size: 1071 3725
BsrFI r'CCGG_y
Cuts at: 2794 2975 3184 2794
Size: 181 209 4406
Fragments arranged by size:
4406 209 181
BsrGI T'GTAC_A
Cuts at: 203 594 1725 203
Size: 391 1131 3274
Fragments arranged by size:
3274 1131 391
BssSI C'ACGA_G
Cuts at: 3067 3425 3067
Size: 358 4438
BstAPI GCAn_nnn'nTGC
Cuts at: 2173 2173
Size: 4796
BstBI TT'CG_AA
Cuts at: 3157 3157
Size: 4796
BstYI r'GATC_y
Cuts at: 1 1821 2288 2311 2646 2892 3904 3990 4002
Size: 1820 467 23 335 246 1012 86 12
Cuts at: 4002 4348 1
Size: 346 449
Fragments arranged by size:
1820 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 304 304
Size: 4796
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2746 2927 3090 2746
Size: 181 163 4452
Fragments arranged by size:
4452 181 163
BtsI GCAGTG_nn'
Cuts at: 2039 2039
Size: 4796
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 151 186 540 575 151
Size: 35 354 35 4372
Fragments arranged by size:
4372 354 35 35
DraI TTT'AAA
Cuts at: 1795 1795
Size: 4796
DrdI GACnn_nn'nnGTC
Cuts at: 2502 3360 2502
Size: 858 3938
EaeI y'GGCC_r
Cuts at: 2381 2555 2946 2973 4318 2381
Size: 174 391 27 1345 2859
Fragments arranged by size:
2859 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2381 2381
Size: 4796
EarI CTCTTCn'nnn_
Cuts at: 443 592 2819 3029 443
Size: 149 2227 210 2210
Fragments arranged by size:
2227 2210 210 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3004 3314 3460 4606 3004
Size: 310 146 1146 3194
Fragments arranged by size:
3194 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1808 1808
Size: 4796
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 467 1098 2621 3053 3800 4068 241
Size: 226 631 1523 432 747 268 969
Fragments arranged by size:
1523 969 747 631 432 268 226
EcoO109I rG'GnC_Cy
Cuts at: 197 1935 197
Size: 1738 3058
EcoRI G'AATT_C
Cuts at: 579 1038 1064 1800 579
Size: 459 26 736 3575
Fragments arranged by size:
3575 736 459 26
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4796
FspI TGC'GCA
Cuts at: 2577 2577
Size: 4796
HaeII r_GCGC'y
Cuts at: 2230 2478 3500 4479 2230
Size: 248 1022 979 2547
Fragments arranged by size:
2547 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 716 748 2638 2668 2670 2700 716
Size: 32 1890 30 2 30 2812
Fragments arranged by size:
2812 1890 32 30 30 2
HincII GTy'rAC
Cuts at: 1127 1379 1835 1127
Size: 252 456 4088
Fragments arranged by size:
4088 456 252
HindIII A'AGCT_T
Cuts at: 566 566
Size: 4796
KasI G'GCGC_C
Cuts at: 2226 2474 2226
Size: 248 4548
KpnI G_GTAC'C
Cuts at: 1816 1816
Size: 4796
MluI A'CGCG_T
Cuts at: 4229 4229
Size: 4796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 266 3467 3651 266
Size: 3201 184 1411
Fragments arranged by size:
3201 1411 184
MscI TGG'CCA
Cuts at: 2557 2557
Size: 4796
MslI CAynn'nnrTG
Cuts at: 764 932 1016 1100 1173 1184 1325 1409 2785
Size: 168 84 84 73 11 141 84 1376
Cuts at: 2785 2912 764
Size: 127 2648
Fragments arranged by size:
2648 1376 168 141 127 84 84 84
73 11
MspA1I CmG'CkG
Cuts at: 1907 2221 2581 3594 3839 1907
Size: 314 360 1013 245 2864
Fragments arranged by size:
2864 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2977 2977
Size: 4796
NarI GG'CG_CC
Cuts at: 2227 2475 2227
Size: 248 4548
NcoI C'CATG_G
Cuts at: 304 304
Size: 4796
NdeI CA'TA_TG
Cuts at: 1170 1170
Size: 4796
NgoMIV G'CCGG_C
Cuts at: 2975 2975
Size: 4796
NsiI A_TGCA'T
Cuts at: 1269 1269
Size: 4796
NspI r_CATG'y
Cuts at: 1271 1849 2349 3256 1271
Size: 578 500 907 2811
Fragments arranged by size:
2811 907 578 500
PacI TTA_AT'TAA
Cuts at: 353 353
Size: 4796
PasI CC'CwG_GG
Cuts at: 120 120
Size: 4796
PciI A'CATG_T
Cuts at: 3252 3252
Size: 4796
PmeI GTTT'AAAC
Cuts at: 1795 1795
Size: 4796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 4764
PpuMI rG'GwC_Cy
Cuts at: 197 197
Size: 4796
PstI C_TGCA'G
Cuts at: 1843 2528 1843
Size: 685 4111
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4796
PvuII CAG'CTG
Cuts at: 2221 2581 2221
Size: 360 4436
RsrII CG'GwC_CG
Cuts at: 2991 2991
Size: 4796
SacI G_AGCT'C
Cuts at: 1810 1810
Size: 4796
SalI G'TCGA_C
Cuts at: 1833 1833
Size: 4796
SapI GCTCTTCn'nnn_
Cuts at: 2819 3029 2819
Size: 210 4586
SbfI CC_TGCA'GG
Cuts at: 1843 1843
Size: 4796
ScaI AGT'ACT
Cuts at: 4313 4313
Size: 4796
SfcI C'TryA_G
Cuts at: 33 228 1839 2524 3517 3708 33
Size: 195 1611 685 993 191 1121
Fragments arranged by size:
1611 1121 993 685 195 191
SfoI GGC'GCC
Cuts at: 2228 2476 2228
Size: 248 4548
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
SmaI CCC'GGG
Cuts at: 1818 1818
Size: 4796
SmlI C'TyrA_G
Cuts at: 161 280 1951 2866 3358 3620 161
Size: 119 1671 915 492 262 1337
Fragments arranged by size:
1671 1337 915 492 262 119
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4796
SphI G_CATG'C
Cuts at: 1849 2349 1849
Size: 500 4296
SspI AAT'ATT
Cuts at: 831 831
Size: 4796
StyI C'CwwG_G
Cuts at: 304 1930 2284 304
Size: 1626 354 2816
Fragments arranged by size:
2816 1626 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 964 2379 2512 2971 3180 964
Size: 1415 133 459 209 2580
Fragments arranged by size:
2580 1415 459 209 133
TatI w'GTAC_w
Cuts at: 203 594 1725 4311 203
Size: 391 1131 2586 688
Fragments arranged by size:
2586 1131 688 391
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 639 671 4313 4345 639
Size: 32 3642 32 1090
Fragments arranged by size:
3642 1090 32 32
Tth111I GACn'n_nGTC
Cuts at: 2593 2593
Size: 4796
XbaI T'CTAG_A
Cuts at: 1827 1827
Size: 4796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2291 2291
Size: 4796
XmaI C'CCGG_G
Cuts at: 1816 1816
Size: 4796
XmnI GAAnn'nnTTC
Cuts at: 275 323 369 437 484 568 746 1027 1111
Size: 48 46 68 47 84 178 281 84
Cuts at: 1111 1195 1363 1430 1531 275
Size: 84 168 67 101 3540
Fragments arranged by size:
3540 281 178 168 101 84 84 84
68 67 48 47 46
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PacI PasI PciI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NheI NotI NruI PflMI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI