Restriction map for
pF1KB5949
(Circular) (Six-base) MAPSORT of: pF1KB5949.seq Check: 528 from: 1 to: 4640
pF1KB5949 4640 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1678 1678
Size: 4640
Acc65I G'GTAC_C
Cuts at: 1656 1656
Size: 4640
AclI AA'CG_TT
Cuts at: 4480 4480
Size: 4640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 923 1403 2465 2897 3644 3912 923
Size: 480 1062 432 747 268 1651
Fragments arranged by size:
1651 1062 747 480 432 268
AflIII A'CryG_T
Cuts at: 1572 1992 3096 4073 1572
Size: 420 1104 977 2139
Fragments arranged by size:
2139 1104 977 420
AgeI A'CCGG_T
Cuts at: 3028 3028
Size: 4640
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 581 581
Size: 4640
AlwNI CAG_nnn'CTG
Cuts at: 318 1146 1945 3512 318
Size: 828 799 1567 1446
Fragments arranged by size:
1567 1446 828 799
ApaLI G'TGCA_C
Cuts at: 791 3410 791
Size: 2619 2021
ApoI r'AATT_y
Cuts at: 433 1235 1644 3993 4249 433
Size: 802 409 2349 256 824
Fragments arranged by size:
2349 824 802 409 256
AseI AT'TA_AT
Cuts at: 20 613 20
Size: 593 4047
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
AvaI C'yCGr_G
Cuts at: 1660 1660
Size: 4640
BamHI G'GATC_C
Cuts at: 1665 1665
Size: 4640
BanI G'GyrC_C
Cuts at: 1656 2070 2318 2353 1656
Size: 414 248 35 3943
Fragments arranged by size:
3943 414 248 35
BanII G_rGCy'C
Cuts at: 508 1654 2684 508
Size: 1146 1030 2464
Fragments arranged by size:
2464 1146 1030
BbeI G_GCGC'C
Cuts at: 2074 2322 2074
Size: 248 4392
BbsI GAAGACnn'nnnn_
Cuts at: 1112 1274 1389 1112
Size: 162 115 4363
Fragments arranged by size:
4363 162 115
BbvCI CC'TCA_GC
Cuts at: 141 141
Size: 4640
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 610 644 610
Size: 34 4606
BciVI GTATCCnnnnn_n'
Cuts at: 2538 3299 4640 2538
Size: 761 1341 2538
Fragments arranged by size:
2538 1341 761
BclI T'GATC_A
Cuts at: 2160 2160
Size: 4640
BglI GCCn_nnn'nGGC
Cuts at: 112 151 112
Size: 39 4601
BglII A'GATC_T
Cuts at: 1155 2155 4192 1155
Size: 1000 2037 1603
Fragments arranged by size:
2037 1603 1000
BlpI GC'TnA_GC
Cuts at: 1752 1752
Size: 4640
Bme1580I G_kGCm'C
Cuts at: 795 2265 2358 3414 795
Size: 1470 93 1056 2021
Fragments arranged by size:
2021 1470 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1852 1933 2268 4412 1852
Size: 81 335 2144 2080
Fragments arranged by size:
2144 2080 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 4640
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 315 440 1480 197
Size: 118 125 1040 3357
Fragments arranged by size:
3357 1040 125 118
Bpu10I CC'TnA_GC
Cuts at: 141 810 141
Size: 669 3971
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1816 2695 3187 3485 1816
Size: 879 492 298 2971
Fragments arranged by size:
2971 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4344 4344
Size: 4640
BsaAI yAC'GTr
Cuts at: 1528 1993 1528
Size: 465 4175
BsaBI GATnn'nnATC
Cuts at: 6 2154 4191 6
Size: 2148 2037 455
Fragments arranged by size:
2148 2037 455
BsaHI Gr'CG_yC
Cuts at: 2071 2319 3021 4517 2071
Size: 248 702 1496 2194
Fragments arranged by size:
2194 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1829 2350 3028 3302 3449 3860 1829
Size: 521 678 274 147 411 2609
Fragments arranged by size:
2609 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1164 2065 2823 3400 3896 1164
Size: 901 758 577 496 1908
Fragments arranged by size:
1908 901 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 1238 120
Size: 1118 3522
BsiEI CG_ry'CG
Cuts at: 104 2182 2228 3436 104
Size: 2078 46 1208 1308
Fragments arranged by size:
2078 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 795 1654 2432 2622 3414 795
Size: 859 778 190 792 2021
Fragments arranged by size:
2021 859 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 508 795 1654 2265 2358 2432 2622 2684 3414
Size: 287 859 611 93 74 190 62 730
Cuts at: 3414 508
Size: 1734
Fragments arranged by size:
1734 859 730 611 287 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3860 3860
Size: 4640
BspHI T'CATG_A
Cuts at: 3816 3816
Size: 4640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1690 1904 2206 2587 1690
Size: 214 302 381 3743
Fragments arranged by size:
3743 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2932 2986 4470 4635 50
Size: 2882 54 1484 165 55
Fragments arranged by size:
2882 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 993 1393 2552 993
Size: 400 1159 3081
Fragments arranged by size:
3081 1159 400
BsrFI r'CCGG_y
Cuts at: 196 2638 2819 3028 196
Size: 2442 181 209 1808
Fragments arranged by size:
2442 1808 209 181
BssSI C'ACGA_G
Cuts at: 2911 3269 2911
Size: 358 4282
BstAPI GCAn_nnn'nTGC
Cuts at: 193 2017 193
Size: 1824 2816
BstBI TT'CG_AA
Cuts at: 3001 3001
Size: 4640
BstEII G'GTnAC_C
Cuts at: 199 199
Size: 4640
BstYI r'GATC_y
Cuts at: 1 314 841 1155 1564 1665 2132 2155 2490
Size: 313 527 314 409 101 467 23 335
Cuts at: 2490 2736 3748 3834 3846 4192 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 527 467 449 409 346 335 314
313 246 101 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4640
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 342 2590 2771 2934 342
Size: 2248 181 163 2048
Fragments arranged by size:
2248 2048 181 163
BtsI GCAGTG_nn'
Cuts at: 1883 1883
Size: 4640
DraI TTT'AAA
Cuts at: 1639 1639
Size: 4640
DrdI GACnn_nn'nnGTC
Cuts at: 2346 3204 2346
Size: 858 3782
EaeI y'GGCC_r
Cuts at: 113 152 168 2225 2399 2790 2817 4162 113
Size: 39 16 2057 174 391 27 1345 591
Fragments arranged by size:
2057 1345 591 391 174 39 27 16
EagI C'GGCC_G
Cuts at: 2225 2225
Size: 4640
EarI CTCTTCn'nnn_
Cuts at: 236 641 2663 2873 236
Size: 405 2022 210 2003
Fragments arranged by size:
2022 2003 405 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2848 3158 3304 4450 2848
Size: 310 146 1146 3038
Fragments arranged by size:
3038 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1652 1652
Size: 4640
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 315 440 923 1403 1480 2465 2897 3644
Size: 118 125 483 480 77 985 432 747
Cuts at: 3644 3912 197
Size: 268 925
Fragments arranged by size:
985 925 747 483 480 432 268 125
118 77
EcoO109I rG'GnC_Cy
Cuts at: 1779 1779
Size: 4640
EcoRI G'AATT_C
Cuts at: 1235 1644 1235
Size: 409 4231
EcoRV GAT'ATC
Cuts at: 1092 1092
Size: 4640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1291 1433 2659 1291
Size: 142 1226 3272
Fragments arranged by size:
3272 1226 142
FspI TGC'GCA
Cuts at: 2421 2421
Size: 4640
HaeII r_GCGC'y
Cuts at: 2074 2322 3344 4323 2074
Size: 248 1022 979 2391
Fragments arranged by size:
2391 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1140 1172 1615 1647 2482 2512 2514 2544 1140
Size: 32 443 32 835 30 2 30 3236
Fragments arranged by size:
3236 835 443 32 32 30 30 2
HincII GTy'rAC
Cuts at: 441 738 926 1679 441
Size: 297 188 753 3402
Fragments arranged by size:
3402 753 297 188
HindIII A'AGCT_T
Cuts at: 697 697
Size: 4640
HpaI GTT'AAC
Cuts at: 441 738 441
Size: 297 4343
KasI G'GCGC_C
Cuts at: 2070 2318 2070
Size: 248 4392
KpnI G_GTAC'C
Cuts at: 1660 1660
Size: 4640
MluI A'CGCG_T
Cuts at: 4073 4073
Size: 4640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 231 3311 3495 231
Size: 3080 184 1376
Fragments arranged by size:
3080 1376 184
MscI TGG'CCA
Cuts at: 2401 2401
Size: 4640
MslI CAynn'nnrTG
Cuts at: 2629 2756 2629
Size: 127 4513
MspA1I CmG'CkG
Cuts at: 217 1168 1751 2065 2425 3438 3683 217
Size: 951 583 314 360 1013 245 1174
Fragments arranged by size:
1174 1013 951 583 360 314 245
NaeI GCC'GGC
Cuts at: 2821 2821
Size: 4640
NarI GG'CG_CC
Cuts at: 2071 2319 2071
Size: 248 4392
NcoI C'CATG_G
Cuts at: 107 107
Size: 4640
NgoMIV G'CCGG_C
Cuts at: 2819 2819
Size: 4640
NspI r_CATG'y
Cuts at: 1576 1583 1693 2193 3100 1576
Size: 7 110 500 907 3116
Fragments arranged by size:
3116 907 500 110 7
PciI A'CATG_T
Cuts at: 1572 3096 1572
Size: 1524 3116
PfoI T'CCnGG_A
Cuts at: 1496 1496
Size: 4640
PmeI GTTT'AAAC
Cuts at: 1639 1639
Size: 4640
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 3779
Size: 32 4608
PstI C_TGCA'G
Cuts at: 633 1687 2372 633
Size: 1054 685 2901
Fragments arranged by size:
2901 1054 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4640
PvuII CAG'CTG
Cuts at: 217 1168 2065 2425 217
Size: 951 897 360 2432
Fragments arranged by size:
2432 951 897 360
RsrII CG'GwC_CG
Cuts at: 2835 2835
Size: 4640
SacI G_AGCT'C
Cuts at: 1654 1654
Size: 4640
SalI G'TCGA_C
Cuts at: 1677 1677
Size: 4640
SapI GCTCTTCn'nnn_
Cuts at: 641 2663 2873 641
Size: 2022 210 2408
Fragments arranged by size:
2408 2022 210
SbfI CC_TGCA'GG
Cuts at: 1687 1687
Size: 4640
ScaI AGT'ACT
Cuts at: 4157 4157
Size: 4640
SfcI C'TryA_G
Cuts at: 33 629 1138 1223 1683 2368 3361 3552 33
Size: 596 509 85 460 685 993 191 1121
Fragments arranged by size:
1121 993 685 596 509 460 191 85
SfoI GGC'GCC
Cuts at: 2072 2320 2072
Size: 248 4392
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4640
SmaI CCC'GGG
Cuts at: 1662 1662
Size: 4640
SmlI C'TyrA_G
Cuts at: 1795 2710 3202 3464 1795
Size: 915 492 262 2971
Fragments arranged by size:
2971 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4640
SphI G_CATG'C
Cuts at: 1693 2193 1693
Size: 500 4140
SspI AAT'ATT
Cuts at: 285 684 285
Size: 399 4241
StyI C'CwwG_G
Cuts at: 107 929 1774 2128 107
Size: 822 845 354 2619
Fragments arranged by size:
2619 845 822 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 218 2223 2356 2815 3024 218
Size: 2005 133 459 209 1834
Fragments arranged by size:
2005 1834 459 209 133
TatI w'GTAC_w
Cuts at: 1491 4155 1491
Size: 2664 1976
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4157 4189 4157
Size: 32 4608
Tth111I GACn'n_nGTC
Cuts at: 2437 2437
Size: 4640
XbaI T'CTAG_A
Cuts at: 1671 1671
Size: 4640
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2135 2135
Size: 4640
XmaI C'CCGG_G
Cuts at: 1660 1660
Size: 4640
XmnI GAAnn'nnTTC
Cuts at: 285 285
Size: 4640
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BfrBI BmgBI BmtI BsaXI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI FseI FspAI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
ZraI