Restriction map for
pF1KB5941
(Circular) (Six-base) MAPSORT of: pF1KB5941.seq Check: 1012 from: 1 to: 4637
pF1KB5941 4637 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1098 1098
Size: 4637
AatII G_ACGT'C
Cuts at: 634 659 634
Size: 25 4612
AccI GT'mk_AC
Cuts at: 1675 1675
Size: 4637
Acc65I G'GTAC_C
Cuts at: 1653 1653
Size: 4637
AclI AA'CG_TT
Cuts at: 4477 4477
Size: 4637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 2462 2894 3641 3909 961
Size: 1501 432 747 268 1689
Fragments arranged by size:
1689 1501 747 432 268
AfeI AGC'GCT
Cuts at: 315 1117 315
Size: 802 3835
AflIII A'CryG_T
Cuts at: 446 1989 3093 4070 446
Size: 1543 1104 977 1013
Fragments arranged by size:
1543 1104 1013 977
AgeI A'CCGG_T
Cuts at: 3025 3025
Size: 4637
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1338 1338
Size: 4637
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 193 225 193
Size: 32 4605
AlwNI CAG_nnn'CTG
Cuts at: 1270 1597 1942 3509 1270
Size: 327 345 1567 2398
Fragments arranged by size:
2398 1567 345 327
ApaI G_GGCC'C
Cuts at: 248 328 1168 248
Size: 80 840 3717
Fragments arranged by size:
3717 840 80
ApaLI G'TGCA_C
Cuts at: 3407 3407
Size: 4637
ApoI r'AATT_y
Cuts at: 1641 3990 4246 1641
Size: 2349 256 2032
Fragments arranged by size:
2349 2032 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4637
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
AvaI C'yCGr_G
Cuts at: 118 239 327 355 966 1657 118
Size: 121 88 28 611 691 3098
Fragments arranged by size:
3098 691 611 121 88 28
BamHI G'GATC_C
Cuts at: 1662 1662
Size: 4637
BanI G'GyrC_C
Cuts at: 180 1653 2067 2315 2350 180
Size: 1473 414 248 35 2467
Fragments arranged by size:
2467 1473 414 248 35
BanII G_rGCy'C
Cuts at: 248 328 939 973 1063 1168 1536 1651 2681
Size: 80 611 34 90 105 368 115 1030
Cuts at: 2681 248
Size: 2204
Fragments arranged by size:
2204 1030 611 368 115 105 90 80
34
BbeI G_GCGC'C
Cuts at: 184 2071 2319 184
Size: 1887 248 2502
Fragments arranged by size:
2502 1887 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 441 475 441
Size: 34 4603
BciVI GTATCCnnnnn_n'
Cuts at: 1197 2535 3296 4637 1197
Size: 1338 761 1341 1197
Fragments arranged by size:
1341 1338 1197 761
BclI T'GATC_A
Cuts at: 2157 2157
Size: 4637
BglI GCCn_nnn'nGGC
Cuts at: 178 232 178
Size: 54 4583
BglII A'GATC_T
Cuts at: 950 2152 4189 950
Size: 1202 2037 1398
Fragments arranged by size:
2037 1398 1202
BlpI GC'TnA_GC
Cuts at: 727 782 1598 1749 727
Size: 55 816 151 3615
Fragments arranged by size:
3615 816 151 55
Bme1580I G_kGCm'C
Cuts at: 248 328 464 1168 1435 2262 2355 3411 248
Size: 80 136 704 267 827 93 1056 1474
Fragments arranged by size:
1474 1056 827 704 267 136 93 80
BmgBI CAC'GTC
Cuts at: 449 449
Size: 4637
BmrI ACTGGGnnnn_n'
Cuts at: 1244 1849 1930 2265 4409 1244
Size: 605 81 335 2144 1472
Fragments arranged by size:
2144 1472 605 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1380 3004 1380
Size: 1624 3013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 825 1195 724
Size: 101 370 4166
Fragments arranged by size:
4166 370 101
Bpu10I CC'TnA_GC
Cuts at: 1436 1515 1436
Size: 79 4558
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1813 2692 3184 3482 1813
Size: 879 492 298 2968
Fragments arranged by size:
2968 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4341 4341
Size: 4637
BsaAI yAC'GTr
Cuts at: 591 1990 591
Size: 1399 3238
BsaBI GATnn'nnATC
Cuts at: 6 1208 2151 4188 6
Size: 1202 943 2037 455
Fragments arranged by size:
2037 1202 943 455
BsaHI Gr'CG_yC
Cuts at: 124 181 545 631 656 838 2068 2316 3018
Size: 57 364 86 25 182 1230 248 702
Cuts at: 3018 4514 124
Size: 1496 247
Fragments arranged by size:
1496 1230 702 364 248 247 182 86
57 25
BsaWI w'CCGG_w
Cuts at: 1826 2347 3025 3299 3446 3857 1826
Size: 521 678 274 147 411 2606
Fragments arranged by size:
2606 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 328 358 811 841 1387 1417 328
Size: 30 453 30 546 30 3548
Fragments arranged by size:
3548 546 453 30 30 30
BseYI C'CCAG_C
Cuts at: 166 316 1121 1348 1399 1406 1590 1612 2062
Size: 150 805 227 51 7 184 22 450
Cuts at: 2062 2820 3397 3893 166
Size: 758 577 496 910
Fragments arranged by size:
910 805 758 577 496 450 227 184
150 51 22 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 1050 370
Size: 680 3957
BsiEI CG_ry'CG
Cuts at: 104 665 2179 2225 3433 104
Size: 561 1514 46 1208 1308
Fragments arranged by size:
1514 1308 1208 561 46
BsiHKAI G_wGCw'C
Cuts at: 539 914 1651 2429 2619 3411 539
Size: 375 737 778 190 792 1765
Fragments arranged by size:
1765 792 778 737 375 190
BsmBI CGTCTCn'nnnn_
Cuts at: 638 663 638
Size: 25 4612
Bsp1286I G_dGCh'C
Cuts at: 248 328 464 539 914 939 973 1063 1168
Size: 80 136 75 375 25 34 90 105
Cuts at: 1168 1435 1536 1651 2262 2355 2429 2619 2681
Size: 267 101 115 611 93 74 190 62
Cuts at: 2681 3411 248
Size: 730 1474
Fragments arranged by size:
1474 730 611 375 267 190 136 115
105 101 93 90 80 75 74 62
34 25
BspEI T'CCGG_A
Cuts at: 3857 3857
Size: 4637
BspHI T'CATG_A
Cuts at: 3813 3813
Size: 4637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 743 1098 1687 1901 2203 2584 743
Size: 355 589 214 302 381 2796
Fragments arranged by size:
2796 589 381 355 302 214
BsrBI CCG'CTC
Cuts at: 50 273 584 2929 2983 4467 4632 50
Size: 223 311 2345 54 1484 165 55
Fragments arranged by size:
2345 1484 311 223 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2549 2549
Size: 4637
BsrFI r'CCGG_y
Cuts at: 664 2635 2816 3025 664
Size: 1971 181 209 2276
Fragments arranged by size:
2276 1971 209 181
BssHII G'CGCG_C
Cuts at: 770 770
Size: 4637
BssSI C'ACGA_G
Cuts at: 404 883 2908 3266 404
Size: 479 2025 358 1775
Fragments arranged by size:
2025 1775 479 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2014 2014
Size: 4637
BstBI TT'CG_AA
Cuts at: 2998 2998
Size: 4637
BstXI CCAn_nnnn'nTGG
Cuts at: 1042 1620 1042
Size: 578 4059
BstYI r'GATC_y
Cuts at: 1 698 950 1662 2129 2152 2487 2733 3745
Size: 697 252 712 467 23 335 246 1012
Cuts at: 3745 3831 3843 4189 1
Size: 86 12 346 449
Fragments arranged by size:
1012 712 697 467 449 346 335 252
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 367 398 551 1295 1420 107
Size: 260 31 153 744 125 3324
Fragments arranged by size:
3324 744 260 153 125 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2587 2768 2931 2587
Size: 181 163 4293
Fragments arranged by size:
4293 181 163
BtsI GCAGTG_nn'
Cuts at: 1145 1194 1284 1880 1145
Size: 49 90 596 3902
Fragments arranged by size:
3902 596 90 49
DraI TTT'AAA
Cuts at: 1636 1636
Size: 4637
DraIII CAC_nnn'GTG
Cuts at: 594 719 594
Size: 125 4512
DrdI GACnn_nn'nnGTC
Cuts at: 2343 3201 2343
Size: 858 3779
EaeI y'GGCC_r
Cuts at: 585 662 1292 2222 2396 2787 2814 4159 585
Size: 77 630 930 174 391 27 1345 1063
Fragments arranged by size:
1345 1063 930 630 391 174 77 27
EagI C'GGCC_G
Cuts at: 662 2222 662
Size: 1560 3077
EarI CTCTTCn'nnn_
Cuts at: 245 1000 2660 2870 245
Size: 755 1660 210 2012
Fragments arranged by size:
2012 1660 755 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2845 3155 3301 4447 2845
Size: 310 146 1146 3035
Fragments arranged by size:
3035 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1649 1649
Size: 4637
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 825 961 1195 2462 2894 3641 3909 724
Size: 101 136 234 1267 432 747 268 1452
Fragments arranged by size:
1452 1267 747 432 268 234 136 101
EcoNI CCTnn'n_nnAGG
Cuts at: 388 388
Size: 4637
EcoO109I rG'GnC_Cy
Cuts at: 244 1083 1164 1165 1257 1267 1776 244
Size: 839 81 1 92 10 509 3105
Fragments arranged by size:
3105 839 509 92 81 10 1
EcoRI G'AATT_C
Cuts at: 1641 1641
Size: 4637
EcoRV GAT'ATC
Cuts at: 505 505
Size: 4637
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1032 2656 1032
Size: 1624 3013
FspI TGC'GCA
Cuts at: 2418 2418
Size: 4637
HaeII r_GCGC'y
Cuts at: 184 317 497 812 1119 2071 2319 3341 4320
Size: 133 180 315 307 952 248 1022 979
Cuts at: 4320 184
Size: 501
Fragments arranged by size:
1022 979 952 501 315 307 248 180
133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 811 843 1291 1323 1387 1419 1498 1530 2479
Size: 32 448 32 64 32 79 32 949
Cuts at: 2479 2509 2511 2541 811
Size: 30 2 30 2907
Fragments arranged by size:
2907 949 448 79 64 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1676 1676
Size: 4637
KasI G'GCGC_C
Cuts at: 180 2067 2315 180
Size: 1887 248 2502
Fragments arranged by size:
2502 1887 248
KpnI G_GTAC'C
Cuts at: 1657 1657
Size: 4637
MluI A'CGCG_T
Cuts at: 4070 4070
Size: 4637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 257 797 1589 3308 3492 257
Size: 540 792 1719 184 1402
Fragments arranged by size:
1719 1402 792 540 184
MscI TGG'CCA
Cuts at: 1294 2398 1294
Size: 1104 3533
MslI CAynn'nnrTG
Cuts at: 524 883 895 2626 2753 524
Size: 359 12 1731 127 2408
Fragments arranged by size:
2408 1731 359 127 12
MspA1I CmG'CkG
Cuts at: 1023 1597 1748 2062 2422 3435 3680 1023
Size: 574 151 314 360 1013 245 1980
Fragments arranged by size:
1980 1013 574 360 314 245 151
NaeI GCC'GGC
Cuts at: 666 2818 666
Size: 2152 2485
NarI GG'CG_CC
Cuts at: 181 2068 2316 181
Size: 1887 248 2502
Fragments arranged by size:
2502 1887 248
NcoI C'CATG_G
Cuts at: 107 367 1295 1420 107
Size: 260 928 125 3324
Fragments arranged by size:
3324 928 260 125
NgoMIV G'CCGG_C
Cuts at: 664 2816 664
Size: 2152 2485
NspI r_CATG'y
Cuts at: 1198 1690 2190 3097 1198
Size: 492 500 907 2738
Fragments arranged by size:
2738 907 500 492
PasI CC'CwG_GG
Cuts at: 1264 1527 1264
Size: 263 4374
PciI A'CATG_T
Cuts at: 3093 3093
Size: 4637
PflMI CCAn_nnn'nTGG
Cuts at: 1270 1270
Size: 4637
PfoI T'CCnGG_A
Cuts at: 197 701 1391 197
Size: 504 690 3443
Fragments arranged by size:
3443 690 504
PmeI GTTT'AAAC
Cuts at: 1636 1636
Size: 4637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 4605
PpuMI rG'GwC_Cy
Cuts at: 1083 1257 1083
Size: 174 4463
PspOMI G'GGCC_C
Cuts at: 244 324 1164 244
Size: 80 840 3717
Fragments arranged by size:
3717 840 80
PspXI vC'TCGA_Gb
Cuts at: 355 355
Size: 4637
PstI C_TGCA'G
Cuts at: 1684 2369 1684
Size: 685 3952
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4637
PvuII CAG'CTG
Cuts at: 1023 2062 2422 1023
Size: 1039 360 3238
Fragments arranged by size:
3238 1039 360
RsrII CG'GwC_CG
Cuts at: 2832 2832
Size: 4637
SacI G_AGCT'C
Cuts at: 1651 1651
Size: 4637
SalI G'TCGA_C
Cuts at: 1674 1674
Size: 4637
SapI GCTCTTCn'nnn_
Cuts at: 245 2660 2870 245
Size: 2415 210 2012
Fragments arranged by size:
2415 2012 210
SbfI CC_TGCA'GG
Cuts at: 1684 1684
Size: 4637
ScaI AGT'ACT
Cuts at: 4154 4154
Size: 4637
SfcI C'TryA_G
Cuts at: 33 336 437 1509 1680 2365 3358 3549 33
Size: 303 101 1072 171 685 993 191 1121
Fragments arranged by size:
1121 1072 993 685 303 191 171 101
SfoI GGC'GCC
Cuts at: 182 2069 2317 182
Size: 1887 248 2502
Fragments arranged by size:
2502 1887 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
SmaI CCC'GGG
Cuts at: 241 329 1659 241
Size: 88 1330 3219
Fragments arranged by size:
3219 1330 88
SmlI C'TyrA_G
Cuts at: 355 1792 2707 3199 3461 355
Size: 1437 915 492 262 1531
Fragments arranged by size:
1531 1437 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4637
SphI G_CATG'C
Cuts at: 1198 1690 2190 1198
Size: 492 500 3645
Fragments arranged by size:
3645 500 492
SrfI GCCC'GGGC
Cuts at: 329 329
Size: 4637
StuI AGG'CCT
Cuts at: 992 992
Size: 4637
StyI C'CwwG_G
Cuts at: 107 367 1295 1420 1536 1771 2125 107
Size: 260 928 125 116 235 354 2619
Fragments arranged by size:
2619 928 354 260 235 125 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 639 1018 2220 2353 2812 3021 639
Size: 379 1202 133 459 209 2255
Fragments arranged by size:
2255 1202 459 379 209 133
TatI w'GTAC_w
Cuts at: 1382 4152 1382
Size: 2770 1867
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 274 306 4154 4186 274
Size: 32 3848 32 725
Fragments arranged by size:
3848 725 32 32
Tth111I GACn'n_nGTC
Cuts at: 2434 2434
Size: 4637
XbaI T'CTAG_A
Cuts at: 1668 1668
Size: 4637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2132 2132
Size: 4637
XhoI C'TCGA_G
Cuts at: 355 355
Size: 4637
XmaI C'CCGG_G
Cuts at: 239 327 1657 239
Size: 88 1330 3219
Fragments arranged by size:
3219 1330 88
XmnI GAAnn'nnTTC
Cuts at: 254 254
Size: 4637
ZraI GAC'GTC
Cuts at: 632 657 632
Size: 25 4612
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AflII AhdI AleI AscI AvrII BaeI BbsI BbvCI
BfrBI BmtI BseRI BsiWI BsmI BsrGI BstEII BstZ17I
Bsu36I ClaI CspCI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SspI SwaI