Restriction map for
pF1KB5935
(Circular) (Six-base) MAPSORT of: pF1KB5935.seq Check: 6599 from: 1 to: 4637
pF1KB5935 4637 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1675 1675
Size: 4637
Acc65I G'GTAC_C
Cuts at: 1653 1653
Size: 4637
AclI AA'CG_TT
Cuts at: 4477 4477
Size: 4637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 866 2462 2894 3641 3909 620
Size: 246 1596 432 747 268 1348
Fragments arranged by size:
1596 1348 747 432 268 246
AflIII A'CryG_T
Cuts at: 404 1989 3093 4070 404
Size: 1585 1104 977 971
Fragments arranged by size:
1585 1104 977 971
AgeI A'CCGG_T
Cuts at: 3025 3025
Size: 4637
AleI CACnn'nnGTG
Cuts at: 739 739
Size: 4637
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 757 757
Size: 4637
AlwNI CAG_nnn'CTG
Cuts at: 1942 3509 1942
Size: 1567 3070
ApaLI G'TGCA_C
Cuts at: 3407 3407
Size: 4637
ApoI r'AATT_y
Cuts at: 856 1557 1641 3990 4246 856
Size: 701 84 2349 256 1247
Fragments arranged by size:
2349 1247 701 256 84
AseI AT'TA_AT
Cuts at: 20 179 20
Size: 159 4478
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
AvaI C'yCGr_G
Cuts at: 1657 1657
Size: 4637
BamHI G'GATC_C
Cuts at: 822 1662 822
Size: 840 3797
BanI G'GyrC_C
Cuts at: 1653 2067 2315 2350 1653
Size: 414 248 35 3940
Fragments arranged by size:
3940 414 248 35
BanII G_rGCy'C
Cuts at: 1651 2681 1651
Size: 1030 3607
BbeI G_GCGC'C
Cuts at: 2071 2319 2071
Size: 248 4389
BbvCI CC'TCA_GC
Cuts at: 674 674
Size: 4637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1525 1559 1525
Size: 34 4603
BciVI GTATCCnnnnn_n'
Cuts at: 2535 3296 4637 2535
Size: 761 1341 2535
Fragments arranged by size:
2535 1341 761
BclI T'GATC_A
Cuts at: 2157 2157
Size: 4637
BfrBI ATG'CAT
Cuts at: 1275 1275
Size: 4637
BglII A'GATC_T
Cuts at: 2152 4189 2152
Size: 2037 2600
BlpI GC'TnA_GC
Cuts at: 1749 1749
Size: 4637
Bme1580I G_kGCm'C
Cuts at: 384 2262 2355 3411 384
Size: 1878 93 1056 1610
Fragments arranged by size:
1878 1610 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1849 1930 2265 4409 1849
Size: 81 335 2144 2077
Fragments arranged by size:
2144 2077 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3004 3004
Size: 4637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 931 506
Size: 425 4212
Bpu10I CC'TnA_GC
Cuts at: 674 674
Size: 4637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1813 2692 3184 3482 1813
Size: 879 492 298 2968
Fragments arranged by size:
2968 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4341 4341
Size: 4637
BsaAI yAC'GTr
Cuts at: 1990 1990
Size: 4637
BsaBI GATnn'nnATC
Cuts at: 6 155 1531 2151 4188 6
Size: 149 1376 620 2037 455
Fragments arranged by size:
2037 1376 620 455 149
BsaHI Gr'CG_yC
Cuts at: 1341 1383 2068 2316 3018 4514 1341
Size: 42 685 248 702 1496 1464
Fragments arranged by size:
1496 1464 702 685 248 42
BsaWI w'CCGG_w
Cuts at: 1826 2347 3025 3299 3446 3857 1826
Size: 521 678 274 147 411 2606
Fragments arranged by size:
2606 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1062 1092 1062
Size: 30 4607
BseYI C'CCAG_C
Cuts at: 2062 2820 3397 3893 2062
Size: 758 577 496 2806
Fragments arranged by size:
2806 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 407
Size: 4637
BsiEI CG_ry'CG
Cuts at: 104 2179 2225 3433 104
Size: 2075 46 1208 1308
Fragments arranged by size:
2075 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 627 1320 1651 2429 2619 3411 627
Size: 693 331 778 190 792 1853
Fragments arranged by size:
1853 792 778 693 331 190
Bsp1286I G_dGCh'C
Cuts at: 384 627 1320 1651 2262 2355 2429 2619 2681
Size: 243 693 331 611 93 74 190 62
Cuts at: 2681 3411 384
Size: 730 1610
Fragments arranged by size:
1610 730 693 611 331 243 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3857 3857
Size: 4637
BspHI T'CATG_A
Cuts at: 3813 3813
Size: 4637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1687 1901 2203 2584 1687
Size: 214 302 381 3740
Fragments arranged by size:
3740 381 302 214
BsrBI CCG'CTC
Cuts at: 50 723 2929 2983 4467 4632 50
Size: 673 2206 54 1484 165 55
Fragments arranged by size:
2206 1484 673 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2549 2549
Size: 4637
BsrFI r'CCGG_y
Cuts at: 2635 2816 3025 2635
Size: 181 209 4247
Fragments arranged by size:
4247 209 181
BsrGI T'GTAC_A
Cuts at: 1351 1351
Size: 4637
BssSI C'ACGA_G
Cuts at: 1577 2908 3266 1577
Size: 1331 358 2948
Fragments arranged by size:
2948 1331 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2014 2014
Size: 4637
BstBI TT'CG_AA
Cuts at: 2998 2998
Size: 4637
BstYI r'GATC_y
Cuts at: 1 822 1266 1662 2129 2152 2487 2733 3745
Size: 821 444 396 467 23 335 246 1012
Cuts at: 3745 3831 3843 4189 1
Size: 86 12 346 449
Fragments arranged by size:
1012 821 467 449 444 396 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1059 1059
Size: 4637
BtgI C'CryG_G
Cuts at: 107 1628 107
Size: 1521 3116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2587 2768 2931 2587
Size: 181 163 4293
Fragments arranged by size:
4293 181 163
BtsI GCAGTG_nn'
Cuts at: 1446 1570 1880 1446
Size: 124 310 4203
Fragments arranged by size:
4203 310 124
DraI TTT'AAA
Cuts at: 1636 1636
Size: 4637
DrdI GACnn_nn'nnGTC
Cuts at: 2343 3201 2343
Size: 858 3779
EaeI y'GGCC_r
Cuts at: 2222 2396 2787 2814 4159 2222
Size: 174 391 27 1345 2700
Fragments arranged by size:
2700 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2222 2222
Size: 4637
EarI CTCTTCn'nnn_
Cuts at: 443 713 918 987 2660 2870 443
Size: 270 205 69 1673 210 2210
Fragments arranged by size:
2210 1673 270 210 205 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2845 3155 3301 4447 2845
Size: 310 146 1146 3035
Fragments arranged by size:
3035 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1649 1649
Size: 4637
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 620 866 931 2462 2894 3641 3909 506
Size: 114 246 65 1531 432 747 268 1234
Fragments arranged by size:
1531 1234 747 432 268 246 114 65
EcoNI CCTnn'n_nnAGG
Cuts at: 638 638
Size: 4637
EcoO109I rG'GnC_Cy
Cuts at: 1776 1776
Size: 4637
EcoRI G'AATT_C
Cuts at: 1641 1641
Size: 4637
EcoRV GAT'ATC
Cuts at: 556 565 1377 556
Size: 9 812 3816
Fragments arranged by size:
3816 812 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 4637
FspI TGC'GCA
Cuts at: 2418 2418
Size: 4637
HaeII r_GCGC'y
Cuts at: 2071 2319 3341 4320 2071
Size: 248 1022 979 2388
Fragments arranged by size:
2388 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 347 379 571 603 988 1020 1062 1094 1224
Size: 32 192 32 385 32 42 32 130
Cuts at: 1224 1256 1576 1608 2479 2509 2511 2541 347
Size: 32 320 32 871 30 2 30 2443
Fragments arranged by size:
2443 871 385 320 192 130 42 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 699 1011 1425 1676 699
Size: 312 414 251 3660
Fragments arranged by size:
3660 414 312 251
HpaI GTT'AAC
Cuts at: 1011 1011
Size: 4637
KasI G'GCGC_C
Cuts at: 2067 2315 2067
Size: 248 4389
KpnI G_GTAC'C
Cuts at: 1657 1657
Size: 4637
MluI A'CGCG_T
Cuts at: 4070 4070
Size: 4637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3308 3492 3308
Size: 184 4453
MscI TGG'CCA
Cuts at: 2398 2398
Size: 4637
MslI CAynn'nnrTG
Cuts at: 181 692 739 1280 2626 2753 181
Size: 511 47 541 1346 127 2065
Fragments arranged by size:
2065 1346 541 511 127 47
MspA1I CmG'CkG
Cuts at: 1748 2062 2422 3435 3680 1748
Size: 314 360 1013 245 2705
Fragments arranged by size:
2705 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2818 2818
Size: 4637
NarI GG'CG_CC
Cuts at: 2068 2316 2068
Size: 248 4389
NcoI C'CATG_G
Cuts at: 107 1628 107
Size: 1521 3116
NgoMIV G'CCGG_C
Cuts at: 2816 2816
Size: 4637
NsiI A_TGCA'T
Cuts at: 1277 1277
Size: 4637
NspI r_CATG'y
Cuts at: 408 748 1690 2190 3097 408
Size: 340 942 500 907 1948
Fragments arranged by size:
1948 942 907 500 340
PciI A'CATG_T
Cuts at: 404 3093 404
Size: 2689 1948
PfoI T'CCnGG_A
Cuts at: 522 522
Size: 4637
PmeI GTTT'AAAC
Cuts at: 1636 1636
Size: 4637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 4605
PstI C_TGCA'G
Cuts at: 1684 2369 1684
Size: 685 3952
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4637
PvuII CAG'CTG
Cuts at: 2062 2422 2062
Size: 360 4277
RsrII CG'GwC_CG
Cuts at: 2832 2832
Size: 4637
SacI G_AGCT'C
Cuts at: 1651 1651
Size: 4637
SalI G'TCGA_C
Cuts at: 1674 1674
Size: 4637
SapI GCTCTTCn'nnn_
Cuts at: 443 713 2660 2870 443
Size: 270 1947 210 2210
Fragments arranged by size:
2210 1947 270 210
SbfI CC_TGCA'GG
Cuts at: 1684 1684
Size: 4637
ScaI AGT'ACT
Cuts at: 1617 4154 1617
Size: 2537 2100
SfcI C'TryA_G
Cuts at: 33 704 1680 2365 3358 3549 33
Size: 671 976 685 993 191 1121
Fragments arranged by size:
1121 993 976 685 671 191
SfoI GGC'GCC
Cuts at: 2069 2317 2069
Size: 248 4389
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
SmaI CCC'GGG
Cuts at: 1659 1659
Size: 4637
SmlI C'TyrA_G
Cuts at: 1792 2707 3199 3461 1792
Size: 915 492 262 2968
Fragments arranged by size:
2968 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4637
SphI G_CATG'C
Cuts at: 1690 2190 1690
Size: 500 4137
SspI AAT'ATT
Cuts at: 1333 1333
Size: 4637
StyI C'CwwG_G
Cuts at: 107 1628 1771 2125 107
Size: 1521 143 354 2619
Fragments arranged by size:
2619 1521 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2220 2353 2812 3021 2220
Size: 133 459 209 3836
Fragments arranged by size:
3836 459 209 133
TatI w'GTAC_w
Cuts at: 684 1351 1615 4152 684
Size: 667 264 2537 1169
Fragments arranged by size:
2537 1169 667 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4154 4186 4154
Size: 32 4605
Tth111I GACn'n_nGTC
Cuts at: 142 2434 142
Size: 2292 2345
XbaI T'CTAG_A
Cuts at: 1263 1668 1263
Size: 405 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2132 2132
Size: 4637
XmaI C'CCGG_G
Cuts at: 1657 1657
Size: 4637
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BglI BmgBI BmtI BseRI BsiWI
BsmI BsmBI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII FseI FspAI HindIII MfeI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI