Restriction map for
pF1KB5923
(Circular) (Six-base) MAPSORT of: pF1KB5923.seq Check: 9287 from: 1 to: 4628
pF1KB5923 4628 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1480 1666 1480
Size: 186 4442
Acc65I G'GTAC_C
Cuts at: 1519 1644 1519
Size: 125 4503
AclI AA'CG_TT
Cuts at: 4468 4468
Size: 4628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 1009 2453 2885 3632 3900 655
Size: 354 1444 432 747 268 1383
Fragments arranged by size:
1444 1383 747 432 354 268
AflII C'TTAA_G
Cuts at: 1270 1270
Size: 4628
AflIII A'CryG_T
Cuts at: 1980 3084 4061 1980
Size: 1104 977 2547
Fragments arranged by size:
2547 1104 977
AgeI A'CCGG_T
Cuts at: 3016 3016
Size: 4628
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1114 1114
Size: 4628
AlwNI CAG_nnn'CTG
Cuts at: 1933 3500 1933
Size: 1567 3061
ApaI G_GGCC'C
Cuts at: 1515 1515
Size: 4628
ApaLI G'TGCA_C
Cuts at: 890 3398 890
Size: 2508 2120
ApoI r'AATT_y
Cuts at: 1066 1632 3981 4237 1066
Size: 566 2349 256 1457
Fragments arranged by size:
2349 1457 566 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4628
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 296 337 851 989 1220 1247 1514 1526 1648
Size: 41 514 138 231 27 267 12 122
Cuts at: 1648 296
Size: 3276
Fragments arranged by size:
3276 514 267 231 138 122 41 27
12
AvrII C'CTAG_G
Cuts at: 526 526
Size: 4628
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 506 539 1318 1351 506
Size: 33 779 33 3783
Fragments arranged by size:
3783 779 33 33
BamHI G'GATC_C
Cuts at: 1653 1653
Size: 4628
BanI G'GyrC_C
Cuts at: 207 1519 1644 2058 2306 2341 207
Size: 1312 125 414 248 35 2494
Fragments arranged by size:
2494 1312 414 248 125 35
BanII G_rGCy'C
Cuts at: 151 244 337 1515 1642 2672 151
Size: 93 93 1178 127 1030 2107
Fragments arranged by size:
2107 1178 1030 127 93 93
BbeI G_GCGC'C
Cuts at: 211 2062 2310 211
Size: 1851 248 2529
Fragments arranged by size:
2529 1851 248
BbsI GAAGACnn'nnnn_
Cuts at: 761 1015 1578 761
Size: 254 563 3811
Fragments arranged by size:
3811 563 254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 306 340 90
Size: 34 182 34 4378
Fragments arranged by size:
4378 182 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2526 3287 4628 2526
Size: 761 1341 2526
Fragments arranged by size:
2526 1341 761
BclI T'GATC_A
Cuts at: 2148 2148
Size: 4628
BglI GCCn_nnn'nGGC
Cuts at: 205 205
Size: 4628
BglII A'GATC_T
Cuts at: 2143 4180 2143
Size: 2037 2591
BlpI GC'TnA_GC
Cuts at: 331 1740 331
Size: 1409 3219
Bme1580I G_kGCm'C
Cuts at: 894 1515 2253 2346 3402 894
Size: 621 738 93 1056 2120
Fragments arranged by size:
2120 1056 738 621 93
BmrI ACTGGGnnnn_n'
Cuts at: 576 1840 1921 2256 4400 576
Size: 1264 81 335 2144 804
Fragments arranged by size:
2144 1264 804 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 2995
Size: 4628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 294 435 1195 209
Size: 85 141 760 3642
Fragments arranged by size:
3642 760 141 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1099 1804 2683 3175 3473 1099
Size: 705 879 492 298 2254
Fragments arranged by size:
2254 879 705 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1253 1523 4332 1253
Size: 270 2809 1549
Fragments arranged by size:
2809 1549 270
BsaAI yAC'GTr
Cuts at: 927 1981 927
Size: 1054 3574
BsaBI GATnn'nnATC
Cuts at: 6 2142 4179 6
Size: 2136 2037 455
Fragments arranged by size:
2136 2037 455
BsaHI Gr'CG_yC
Cuts at: 208 274 1542 2059 2307 3009 4505 208
Size: 66 1268 517 248 702 1496 331
Fragments arranged by size:
1496 1268 702 517 331 248 66
BsaWI w'CCGG_w
Cuts at: 962 1817 2338 3016 3290 3437 3848 962
Size: 855 521 678 274 147 411 1742
Fragments arranged by size:
1742 855 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 522 522
Size: 4628
BseYI C'CCAG_C
Cuts at: 256 1110 1151 1560 2053 2811 3388 3884 256
Size: 854 41 409 493 758 577 496 1000
Fragments arranged by size:
1000 854 758 577 496 493 409 41
BsiEI CG_ry'CG
Cuts at: 104 131 2170 2216 3424 104
Size: 27 2039 46 1208 1308
Fragments arranged by size:
2039 1308 1208 46 27
BsiHKAI G_wGCw'C
Cuts at: 151 421 894 1358 1642 2420 2610 3402 151
Size: 270 473 464 284 778 190 792 1377
Fragments arranged by size:
1377 792 778 473 464 284 270 190
BsmI GAATG_Cn'
Cuts at: 825 825
Size: 4628
BsmBI CGTCTCn'nnnn_
Cuts at: 1549 1549
Size: 4628
Bsp1286I G_dGCh'C
Cuts at: 151 244 337 421 894 1358 1515 1642 2253
Size: 93 93 84 473 464 157 127 611
Cuts at: 2253 2346 2420 2610 2672 3402 151
Size: 93 74 190 62 730 1377
Fragments arranged by size:
1377 730 611 473 464 190 157 127
93 93 93 84 74 62
BspEI T'CCGG_A
Cuts at: 962 3848 962
Size: 2886 1742
BspHI T'CATG_A
Cuts at: 3804 3804
Size: 4628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 197 917 1466 1678 1892 2194 2575 197
Size: 720 549 212 214 302 381 2250
Fragments arranged by size:
2250 720 549 381 302 214 212
BsrBI CCG'CTC
Cuts at: 50 223 263 858 2920 2974 4458 4623 50
Size: 173 40 595 2062 54 1484 165 55
Fragments arranged by size:
2062 1484 595 173 165 55 54 40
BsrDI GCAATG_nn'
Cuts at: 2540 2540
Size: 4628
BsrFI r'CCGG_y
Cuts at: 1147 2626 2807 3016 1147
Size: 1479 181 209 2759
Fragments arranged by size:
2759 1479 209 181
BssSI C'ACGA_G
Cuts at: 790 2899 3257 790
Size: 2109 358 2161
Fragments arranged by size:
2161 2109 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2005 2005
Size: 4628
BstBI TT'CG_AA
Cuts at: 2989 2989
Size: 4628
BstEII G'GTnAC_C
Cuts at: 1242 1242
Size: 4628
BstXI CCAn_nnnn'nTGG
Cuts at: 1110 1110
Size: 4628
BstYI r'GATC_y
Cuts at: 1 1179 1653 2120 2143 2478 2724 3736 3822
Size: 1178 474 467 23 335 246 1012 86
Cuts at: 3822 3834 4180 1
Size: 12 346 449
Fragments arranged by size:
1178 1012 474 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1481 1481
Size: 4628
Bsu36I CC'TnA_GG
Cuts at: 1322 1322
Size: 4628
BtgI C'CryG_G
Cuts at: 131 1533 131
Size: 1402 3226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 613 2578 2759 2922 613
Size: 1965 181 163 2319
Fragments arranged by size:
2319 1965 181 163
BtsI GCAGTG_nn'
Cuts at: 1068 1356 1871 1068
Size: 288 515 3825
Fragments arranged by size:
3825 515 288
DraI TTT'AAA
Cuts at: 1627 1627
Size: 4628
DraIII CAC_nnn'GTG
Cuts at: 144 873 144
Size: 729 3899
DrdI GACnn_nn'nnGTC
Cuts at: 1582 2334 3192 1582
Size: 752 858 3018
Fragments arranged by size:
3018 858 752
EaeI y'GGCC_r
Cuts at: 128 864 929 1229 2213 2387 2778 2805 4150
Size: 736 65 300 984 174 391 27 1345
Cuts at: 4150 128
Size: 606
Fragments arranged by size:
1345 984 736 606 391 300 174 65
27
EagI C'GGCC_G
Cuts at: 128 2213 128
Size: 2085 2543
EarI CTCTTCn'nnn_
Cuts at: 2651 2861 2651
Size: 210 4418
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 152 611 2836 3146 3292 4438 152
Size: 459 2225 310 146 1146 342
Fragments arranged by size:
2225 1146 459 342 310 146
EcoICRI GAG'CTC
Cuts at: 149 1640 149
Size: 1491 3137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 294 435 655 1009 1195 2453 2885 3632
Size: 85 141 220 354 186 1258 432 747
Cuts at: 3632 3900 209
Size: 268 937
Fragments arranged by size:
1258 937 747 432 354 268 220 186
141 85
EcoNI CCTnn'n_nnAGG
Cuts at: 539 539
Size: 4628
EcoO109I rG'GnC_Cy
Cuts at: 570 654 675 716 1511 1767 570
Size: 84 21 41 795 256 3431
Fragments arranged by size:
3431 795 256 84 41 21
EcoRI G'AATT_C
Cuts at: 1066 1632 1066
Size: 566 4062
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 4628
FspI TGC'GCA
Cuts at: 2409 2409
Size: 4628
HaeII r_GCGC'y
Cuts at: 161 211 235 1017 2062 2310 3332 4311 161
Size: 50 24 782 1045 248 1022 979 478
Fragments arranged by size:
1045 1022 979 782 478 248 50 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1564 1596 2470 2500 2502 2532 1564
Size: 32 874 30 2 30 3660
Fragments arranged by size:
3660 874 32 30 30 2
HincII GTy'rAC
Cuts at: 1667 1667
Size: 4628
KasI G'GCGC_C
Cuts at: 207 2058 2306 207
Size: 1851 248 2529
Fragments arranged by size:
2529 1851 248
KpnI G_GTAC'C
Cuts at: 1523 1648 1523
Size: 125 4503
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 484 3299 3483 484
Size: 2815 184 1629
Fragments arranged by size:
2815 1629 184
MscI TGG'CCA
Cuts at: 866 931 1231 2389 866
Size: 65 300 1158 3105
Fragments arranged by size:
3105 1158 300 65
MslI CAynn'nnrTG
Cuts at: 982 1240 2617 2744 982
Size: 258 1377 127 2866
Fragments arranged by size:
2866 1377 258 127
MspA1I CmG'CkG
Cuts at: 330 1029 1739 2053 2413 3426 3671 330
Size: 699 710 314 360 1013 245 1287
Fragments arranged by size:
1287 1013 710 699 360 314 245
NaeI GCC'GGC
Cuts at: 2809 2809
Size: 4628
NarI GG'CG_CC
Cuts at: 208 2059 2307 208
Size: 1851 248 2529
Fragments arranged by size:
2529 1851 248
NgoMIV G'CCGG_C
Cuts at: 2807 2807
Size: 4628
NspI r_CATG'y
Cuts at: 589 1681 2181 3088 589
Size: 1092 500 907 2129
Fragments arranged by size:
2129 1092 907 500
PasI CC'CwG_GG
Cuts at: 1599 1599
Size: 4628
PciI A'CATG_T
Cuts at: 3084 3084
Size: 4628
PflMI CCAn_nnn'nTGG
Cuts at: 834 873 1500 834
Size: 39 627 3962
Fragments arranged by size:
3962 627 39
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 3767
Size: 32 4596
PpuMI rG'GwC_Cy
Cuts at: 654 675 716 654
Size: 21 41 4566
Fragments arranged by size:
4566 41 21
PspOMI G'GGCC_C
Cuts at: 1511 1511
Size: 4628
PspXI vC'TCGA_Gb
Cuts at: 296 296
Size: 4628
PstI C_TGCA'G
Cuts at: 329 1282 1675 2360 329
Size: 953 393 685 2597
Fragments arranged by size:
2597 953 685 393
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 330 1029 2053 2413 330
Size: 699 1024 360 2545
Fragments arranged by size:
2545 1024 699 360
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 151 1642 151
Size: 1491 3137
SalI G'TCGA_C
Cuts at: 1665 1665
Size: 4628
SanDI GG'GwC_CC
Cuts at: 716 716
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 2651 2861 2651
Size: 210 4418
SbfI CC_TGCA'GG
Cuts at: 1675 1675
Size: 4628
ScaI AGT'ACT
Cuts at: 4145 4145
Size: 4628
SexAI A'CCwGG_T
Cuts at: 140 656 140
Size: 516 4112
SfcI C'TryA_G
Cuts at: 33 325 1278 1671 2356 3349 3540 33
Size: 292 953 393 685 993 191 1121
Fragments arranged by size:
1121 993 953 685 393 292 191
SfoI GGC'GCC
Cuts at: 209 2060 2308 209
Size: 1851 248 2529
Fragments arranged by size:
2529 1851 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SmaI CCC'GGG
Cuts at: 339 853 991 1516 1650 339
Size: 514 138 525 134 3317
Fragments arranged by size:
3317 525 514 138 134
SmlI C'TyrA_G
Cuts at: 296 1078 1270 1783 2698 3190 3452 296
Size: 782 192 513 915 492 262 1472
Fragments arranged by size:
1472 915 782 513 492 262 192
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 589 1681 2181 589
Size: 1092 500 3036
Fragments arranged by size:
3036 1092 500
StyI C'CwwG_G
Cuts at: 526 1103 1762 2116 526
Size: 577 659 354 3038
Fragments arranged by size:
3038 659 577 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 330 1087 2211 2344 2803 3012 330
Size: 757 1124 133 459 209 1946
Fragments arranged by size:
1946 1124 757 459 209 133
TatI w'GTAC_w
Cuts at: 4143 4143
Size: 4628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4145 4177 4145
Size: 32 4596
Tth111I GACn'n_nGTC
Cuts at: 2425 2425
Size: 4628
XbaI T'CTAG_A
Cuts at: 1659 1659
Size: 4628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1557 2123 1557
Size: 566 4062
XhoI C'TCGA_G
Cuts at: 296 296
Size: 4628
XmaI C'CCGG_G
Cuts at: 337 851 989 1514 1648 337
Size: 514 138 525 134 3317
Fragments arranged by size:
3317 525 514 138 134
XmnI GAAnn'nnTTC
Cuts at: 823 977 823
Size: 154 4474
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI AscI BbvCI
BfrBI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsrGI
BssHII ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI