Restriction map for
pF1KB5922
(Circular) (Six-base) MAPSORT of: pF1KB5922.seq Check: 2505 from: 1 to: 4712
pF1KB5922 4712 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1750 1750
Size: 4712
Acc65I G'GTAC_C
Cuts at: 1700 1728 1700
Size: 28 4684
AclI AA'CG_TT
Cuts at: 4552 4552
Size: 4712
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 1605 2537 2969 3716 3984 404
Size: 1201 932 432 747 268 1132
Fragments arranged by size:
1201 1132 932 747 432 268
AflIII A'CryG_T
Cuts at: 2064 3168 4145 2064
Size: 1104 977 2631
Fragments arranged by size:
2631 1104 977
AgeI A'CCGG_T
Cuts at: 3100 3100
Size: 4712
AhdI GACnn_n'nnGTC
Cuts at: 492 1593 492
Size: 1101 3611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1021 1021
Size: 4712
AlwNI CAG_nnn'CTG
Cuts at: 1408 2017 3584 1408
Size: 609 1567 2536
Fragments arranged by size:
2536 1567 609
ApaLI G'TGCA_C
Cuts at: 1677 3482 1677
Size: 1805 2907
ApoI r'AATT_y
Cuts at: 1353 1716 4065 4321 1353
Size: 363 2349 256 1744
Fragments arranged by size:
2349 1744 363 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4712
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
AvaI C'yCGr_G
Cuts at: 931 1732 931
Size: 801 3911
BamHI G'GATC_C
Cuts at: 1737 1737
Size: 4712
BanI G'GyrC_C
Cuts at: 291 516 1700 1728 2142 2390 2425 291
Size: 225 1184 28 414 248 35 2578
Fragments arranged by size:
2578 1184 414 248 225 35 28
BanII G_rGCy'C
Cuts at: 240 1123 1726 2756 240
Size: 883 603 1030 2196
Fragments arranged by size:
2196 1030 883 603
BbeI G_GCGC'C
Cuts at: 2146 2394 2146
Size: 248 4464
BbsI GAAGACnn'nnnn_
Cuts at: 443 443
Size: 4712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 148 182 148
Size: 34 4678
BciVI GTATCCnnnnn_n'
Cuts at: 2610 3371 4712 2610
Size: 761 1341 2610
Fragments arranged by size:
2610 1341 761
BclI T'GATC_A
Cuts at: 2232 2232
Size: 4712
BglII A'GATC_T
Cuts at: 1141 2227 4264 1141
Size: 1086 2037 1589
Fragments arranged by size:
2037 1589 1086
BlpI GC'TnA_GC
Cuts at: 1824 1824
Size: 4712
Bme1580I G_kGCm'C
Cuts at: 128 1681 2337 2430 3486 128
Size: 1553 656 93 1056 1354
Fragments arranged by size:
1553 1354 1056 656 93
BmrI ACTGGGnnnn_n'
Cuts at: 1699 1924 2005 2340 4484 1699
Size: 225 81 335 2144 1927
Fragments arranged by size:
2144 1927 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3079 3079
Size: 4712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1211 1211
Size: 4712
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1238 1888 2767 3259 3557 1238
Size: 650 879 492 298 2393
Fragments arranged by size:
2393 879 650 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 675 1080 4416 675
Size: 405 3336 971
Fragments arranged by size:
3336 971 405
BsaAI yAC'GTr
Cuts at: 2065 2065
Size: 4712
BsaBI GATnn'nnATC
Cuts at: 6 1079 2226 4263 6
Size: 1073 1147 2037 455
Fragments arranged by size:
2037 1147 1073 455
BsaHI Gr'CG_yC
Cuts at: 2143 2391 3093 4589 2143
Size: 248 702 1496 2266
Fragments arranged by size:
2266 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1901 2422 3100 3374 3521 3932 1901
Size: 521 678 274 147 411 2681
Fragments arranged by size:
2681 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 601 631 813 843 601
Size: 30 182 30 4470
Fragments arranged by size:
4470 182 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 354 357 520 354
Size: 3 163 4546
Fragments arranged by size:
4546 163 3
BseYI C'CCAG_C
Cuts at: 149 656 763 1097 2137 2895 3472 3968 149
Size: 507 107 334 1040 758 577 496 893
Fragments arranged by size:
1040 893 758 577 507 496 334 107
BsiEI CG_ry'CG
Cuts at: 104 633 717 2254 2300 3508 104
Size: 529 84 1537 46 1208 1308
Fragments arranged by size:
1537 1308 1208 529 84 46
BsiHKAI G_wGCw'C
Cuts at: 1123 1681 1726 2504 2694 3486 1123
Size: 558 45 778 190 792 2349
Fragments arranged by size:
2349 792 778 558 190 45
BsmI GAATG_Cn'
Cuts at: 1195 1195
Size: 4712
Bsp1286I G_dGCh'C
Cuts at: 128 240 1123 1681 1726 2337 2430 2504 2694
Size: 112 883 558 45 611 93 74 190
Cuts at: 2694 2756 3486 128
Size: 62 730 1354
Fragments arranged by size:
1354 883 730 611 558 190 112 93
74 62 45
BspEI T'CCGG_A
Cuts at: 3932 3932
Size: 4712
BspHI T'CATG_A
Cuts at: 3888 3888
Size: 4712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1762 1976 2278 2659 1762
Size: 214 302 381 3815
Fragments arranged by size:
3815 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3004 3058 4542 4707 50
Size: 2954 54 1484 165 55
Fragments arranged by size:
2954 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2624 2624
Size: 4712
BsrFI r'CCGG_y
Cuts at: 632 712 2710 2891 3100 632
Size: 80 1998 181 209 2244
Fragments arranged by size:
2244 1998 209 181 80
BsrGI T'GTAC_A
Cuts at: 623 1499 623
Size: 876 3836
BssSI C'ACGA_G
Cuts at: 2983 3341 2983
Size: 358 4354
BstAPI GCAn_nnn'nTGC
Cuts at: 1408 2089 1408
Size: 681 4031
BstBI TT'CG_AA
Cuts at: 3073 3073
Size: 4712
BstXI CCAn_nnnn'nTGG
Cuts at: 232 232
Size: 4712
BstYI r'GATC_y
Cuts at: 1 1141 1526 1737 2204 2227 2562 2808 3820
Size: 1140 385 211 467 23 335 246 1012
Cuts at: 3820 3906 3918 4264 1
Size: 86 12 346 449
Fragments arranged by size:
1140 1012 467 449 385 346 335 246
211 86 23 12
BtgI C'CryG_G
Cuts at: 107 373 521 977 1340 107
Size: 266 148 456 363 3479
Fragments arranged by size:
3479 456 363 266 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2662 2843 3006 2662
Size: 181 163 4368
Fragments arranged by size:
4368 181 163
BtsI GCAGTG_nn'
Cuts at: 218 730 1955 218
Size: 512 1225 2975
Fragments arranged by size:
2975 1225 512
ClaI AT'CG_AT
Cuts at: 230 230
Size: 4712
DraI TTT'AAA
Cuts at: 1711 1711
Size: 4712
DraIII CAC_nnn'GTG
Cuts at: 1228 1228
Size: 4712
DrdI GACnn_nn'nnGTC
Cuts at: 2418 3276 2418
Size: 858 3854
EaeI y'GGCC_r
Cuts at: 689 714 2297 2471 2862 2889 4234 689
Size: 25 1583 174 391 27 1345 1167
Fragments arranged by size:
1583 1345 1167 391 174 27 25
EagI C'GGCC_G
Cuts at: 714 2297 714
Size: 1583 3129
EarI CTCTTCn'nnn_
Cuts at: 329 380 398 506 1050 1343 1361 2735 2945
Size: 51 18 108 544 293 18 1374 210
Cuts at: 2945 329
Size: 2096
Fragments arranged by size:
2096 1374 544 293 210 108 51 18
18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2920 3230 3376 4522 2920
Size: 310 146 1146 3110
Fragments arranged by size:
3110 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1121 1724 1121
Size: 603 4109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 1211 1605 2537 2969 3716 3984 404
Size: 807 394 932 432 747 268 1132
Fragments arranged by size:
1132 932 807 747 432 394 268
EcoO109I rG'GnC_Cy
Cuts at: 141 153 1258 1851 141
Size: 12 1105 593 3002
Fragments arranged by size:
3002 1105 593 12
EcoRI G'AATT_C
Cuts at: 1353 1716 1353
Size: 363 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1429 2731 1429
Size: 1302 3410
FspI TGC'GCA
Cuts at: 2493 2493
Size: 4712
HaeII r_GCGC'y
Cuts at: 2146 2394 3416 4395 2146
Size: 248 1022 979 2463
Fragments arranged by size:
2463 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 478 510 892 924 1262 1294 1518 1550 1579
Size: 32 382 32 338 32 224 32 29
Cuts at: 1579 1611 2554 2584 2586 2616 478
Size: 32 943 30 2 30 2574
Fragments arranged by size:
2574 943 382 338 224 32 32 32
32 32 30 30 29 2
HincII GTy'rAC
Cuts at: 1751 1751
Size: 4712
KasI G'GCGC_C
Cuts at: 2142 2390 2142
Size: 248 4464
KpnI G_GTAC'C
Cuts at: 1704 1732 1704
Size: 28 4684
MluI A'CGCG_T
Cuts at: 4145 4145
Size: 4712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 638 1148 3383 3567 638
Size: 510 2235 184 1783
Fragments arranged by size:
2235 1783 510 184
MscI TGG'CCA
Cuts at: 2473 2473
Size: 4712
MslI CAynn'nnrTG
Cuts at: 230 2701 2828 230
Size: 2471 127 2114
Fragments arranged by size:
2471 2114 127
MspA1I CmG'CkG
Cuts at: 375 919 1342 1823 2137 2497 3510 3755 375
Size: 544 423 481 314 360 1013 245 1332
Fragments arranged by size:
1332 1013 544 481 423 360 314 245
NaeI GCC'GGC
Cuts at: 714 2893 714
Size: 2179 2533
NarI GG'CG_CC
Cuts at: 2143 2391 2143
Size: 248 4464
NcoI C'CATG_G
Cuts at: 107 107
Size: 4712
NdeI CA'TA_TG
Cuts at: 1482 1482
Size: 4712
NgoMIV G'CCGG_C
Cuts at: 712 2891 712
Size: 2179 2533
NspI r_CATG'y
Cuts at: 1188 1693 1765 2265 3172 1188
Size: 505 72 500 907 2728
Fragments arranged by size:
2728 907 505 500 72
PciI A'CATG_T
Cuts at: 3168 3168
Size: 4712
PflMI CCAn_nnn'nTGG
Cuts at: 1036 1036
Size: 4712
PmeI GTTT'AAAC
Cuts at: 1711 1711
Size: 4712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 4680
PpuMI rG'GwC_Cy
Cuts at: 141 153 141
Size: 12 4700
PstI C_TGCA'G
Cuts at: 578 1698 1759 2444 578
Size: 1120 61 685 2846
Fragments arranged by size:
2846 1120 685 61
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4712
PvuII CAG'CTG
Cuts at: 2137 2497 2137
Size: 360 4352
RsrII CG'GwC_CG
Cuts at: 2907 2907
Size: 4712
SacI G_AGCT'C
Cuts at: 1123 1726 1123
Size: 603 4109
SacII CC_GC'GG
Cuts at: 376 1343 376
Size: 967 3745
SalI G'TCGA_C
Cuts at: 1749 1749
Size: 4712
SanDI GG'GwC_CC
Cuts at: 153 153
Size: 4712
SapI GCTCTTCn'nnn_
Cuts at: 2735 2945 2735
Size: 210 4502
SbfI CC_TGCA'GG
Cuts at: 1759 1759
Size: 4712
ScaI AGT'ACT
Cuts at: 4229 4229
Size: 4712
SfcI C'TryA_G
Cuts at: 33 574 1694 1755 2440 3433 3624 33
Size: 541 1120 61 685 993 191 1121
Fragments arranged by size:
1121 1120 993 685 541 191 61
SfoI GGC'GCC
Cuts at: 2144 2392 2144
Size: 248 4464
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
SmaI CCC'GGG
Cuts at: 1734 1734
Size: 4712
SmlI C'TyrA_G
Cuts at: 1253 1867 2782 3274 3536 1253
Size: 614 915 492 262 2429
Fragments arranged by size:
2429 915 614 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4712
SphI G_CATG'C
Cuts at: 1693 1765 2265 1693
Size: 72 500 4140
Fragments arranged by size:
4140 500 72
StyI C'CwwG_G
Cuts at: 107 1846 2200 107
Size: 1739 354 2619
Fragments arranged by size:
2619 1739 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 699 2295 2428 2887 3096 699
Size: 1596 133 459 209 2315
Fragments arranged by size:
2315 1596 459 209 133
TatI w'GTAC_w
Cuts at: 623 1499 4227 623
Size: 876 2728 1108
Fragments arranged by size:
2728 1108 876
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 813 845 4229 4261 813
Size: 32 3384 32 1264
Fragments arranged by size:
3384 1264 32 32
Tth111I GACn'n_nGTC
Cuts at: 2509 2509
Size: 4712
XbaI T'CTAG_A
Cuts at: 1743 1743
Size: 4712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1037 2207 1037
Size: 1170 3542
XmaI C'CCGG_G
Cuts at: 1732 1732
Size: 4712
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI