Restriction map for
pF1KB5911
(Circular) (Six-base) MAPSORT of: pF1KB5911.seq Check: 3984 from: 1 to: 4625
pF1KB5911 4625 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1013 1280 1284 1013
Size: 267 4 4354
Fragments arranged by size:
4354 267 4
AccI GT'mk_AC
Cuts at: 167 1663 167
Size: 1496 3129
Acc65I G'GTAC_C
Cuts at: 1641 1641
Size: 4625
AclI AA'CG_TT
Cuts at: 4465 4465
Size: 4625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 2450 2882 3629 3897 424
Size: 2026 432 747 268 1152
Fragments arranged by size:
2026 1152 747 432 268
AfeI AGC'GCT
Cuts at: 1140 1140
Size: 4625
AflII C'TTAA_G
Cuts at: 640 640
Size: 4625
AflIII A'CryG_T
Cuts at: 1977 3081 4058 1977
Size: 1104 977 2544
Fragments arranged by size:
2544 1104 977
AgeI A'CCGG_T
Cuts at: 3013 3013
Size: 4625
AleI CACnn'nnGTG
Cuts at: 1459 1459
Size: 4625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 415 1288 415
Size: 873 3752
AlwNI CAG_nnn'CTG
Cuts at: 1226 1236 1320 1437 1930 3497 1226
Size: 10 84 117 493 1567 2354
Fragments arranged by size:
2354 1567 493 117 84 10
ApaI G_GGCC'C
Cuts at: 355 1611 355
Size: 1256 3369
ApaLI G'TGCA_C
Cuts at: 3395 3395
Size: 4625
ApoI r'AATT_y
Cuts at: 1629 3978 4234 1629
Size: 2349 256 2020
Fragments arranged by size:
2349 2020 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
AvaI C'yCGr_G
Cuts at: 669 1186 1585 1645 669
Size: 517 399 60 3649
Fragments arranged by size:
3649 517 399 60
BamHI G'GATC_C
Cuts at: 1650 1650
Size: 4625
BanI G'GyrC_C
Cuts at: 241 304 443 1113 1268 1641 2055 2303 2338
Size: 63 139 670 155 373 414 248 35
Cuts at: 2338 241
Size: 2528
Fragments arranged by size:
2528 670 414 373 248 155 139 63
35
BanII G_rGCy'C
Cuts at: 355 521 569 800 1052 1611 1639 2669 355
Size: 166 48 231 252 559 28 1030 2311
Fragments arranged by size:
2311 1030 559 252 231 166 48 28
BbeI G_GCGC'C
Cuts at: 245 447 2059 2307 245
Size: 202 1612 248 2563
Fragments arranged by size:
2563 1612 248 202
BbsI GAAGACnn'nnnn_
Cuts at: 130 130
Size: 4625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 800 834 800
Size: 34 4591
BciVI GTATCCnnnnn_n'
Cuts at: 823 2523 3284 4625 823
Size: 1700 761 1341 823
Fragments arranged by size:
1700 1341 823 761
BclI T'GATC_A
Cuts at: 2145 2145
Size: 4625
BglI GCCn_nnn'nGGC
Cuts at: 917 1014 1281 1507 917
Size: 97 267 226 4035
Fragments arranged by size:
4035 267 226 97
BglII A'GATC_T
Cuts at: 2140 4177 2140
Size: 2037 2588
BlpI GC'TnA_GC
Cuts at: 1570 1737 1570
Size: 167 4458
Bme1580I G_kGCm'C
Cuts at: 355 1118 1611 2250 2343 3399 355
Size: 763 493 639 93 1056 1581
Fragments arranged by size:
1581 1056 763 639 493 93
BmgBI CAC'GTC
Cuts at: 128 128
Size: 4625
BmrI ACTGGGnnnn_n'
Cuts at: 1837 1918 2253 4397 1837
Size: 81 335 2144 2065
Fragments arranged by size:
2144 2065 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 984 2992 984
Size: 2008 2617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1018 1054 1153 1186 1263 568
Size: 450 36 99 33 77 3930
Fragments arranged by size:
3930 450 99 77 36 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1801 2680 3172 3470 1801
Size: 879 492 298 2956
Fragments arranged by size:
2956 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4329 4329
Size: 4625
BsaAI yAC'GTr
Cuts at: 600 1978 600
Size: 1378 3247
BsaBI GATnn'nnATC
Cuts at: 6 2139 4176 6
Size: 2133 2037 455
Fragments arranged by size:
2133 2037 455
BsaHI Gr'CG_yC
Cuts at: 164 242 444 2056 2304 3006 4502 164
Size: 78 202 1612 248 702 1496 287
Fragments arranged by size:
1612 1496 702 287 248 202 78
BsaWI w'CCGG_w
Cuts at: 1196 1814 2335 3013 3287 3434 3845 1196
Size: 618 521 678 274 147 411 1976
Fragments arranged by size:
1976 678 618 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 490 520 757 787 1261 1291 1566 1596 490
Size: 30 237 30 474 30 275 30 3519
Fragments arranged by size:
3519 474 275 237 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1432 1432
Size: 4625
BseYI C'CCAG_C
Cuts at: 1260 1392 1527 2050 2808 3385 3881 1260
Size: 132 135 523 758 577 496 2004
Fragments arranged by size:
2004 758 577 523 496 135 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 1437 343
Size: 1094 3531
BsiEI CG_ry'CG
Cuts at: 104 251 2167 2213 3421 104
Size: 147 1916 46 1208 1308
Fragments arranged by size:
1916 1308 1208 147 46
BsiHKAI G_wGCw'C
Cuts at: 203 521 1004 1576 1639 2417 2607 3399 203
Size: 318 483 572 63 778 190 792 1429
Fragments arranged by size:
1429 792 778 572 483 318 190 63
Bsp1286I G_dGCh'C
Cuts at: 203 355 521 569 800 1004 1052 1118 1576
Size: 152 166 48 231 204 48 66 458
Cuts at: 1576 1611 1639 2250 2343 2417 2607 2669 3399
Size: 35 28 611 93 74 190 62 730
Cuts at: 3399 203
Size: 1429
Fragments arranged by size:
1429 730 611 458 231 204 190 166
152 93 74 66 62 48 48 35
28
BspEI T'CCGG_A
Cuts at: 3845 3845
Size: 4625
BspHI T'CATG_A
Cuts at: 3801 3801
Size: 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 632 761 1013 1067 1280 1284 1675 1889 2191
Size: 129 252 54 213 4 391 214 302
Cuts at: 2191 2572 632
Size: 381 2685
Fragments arranged by size:
2685 391 381 302 252 214 213 129
54 4
BsrBI CCG'CTC
Cuts at: 50 2917 2971 4455 4620 50
Size: 2867 54 1484 165 55
Fragments arranged by size:
2867 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2537 2537
Size: 4625
BsrFI r'CCGG_y
Cuts at: 875 2623 2804 3013 875
Size: 1748 181 209 2487
Fragments arranged by size:
2487 1748 209 181
BssHII G'CGCG_C
Cuts at: 1245 1491 1245
Size: 246 4379
BssSI C'ACGA_G
Cuts at: 715 2896 3254 715
Size: 2181 358 2086
Fragments arranged by size:
2181 2086 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1226 2002 1226
Size: 776 3849
BstBI TT'CG_AA
Cuts at: 2986 2986
Size: 4625
BstEII G'GTnAC_C
Cuts at: 276 726 276
Size: 450 4175
BstXI CCAn_nnnn'nTGG
Cuts at: 273 273
Size: 4625
BstYI r'GATC_y
Cuts at: 1 1650 2117 2140 2475 2721 3733 3819 3831
Size: 1649 467 23 335 246 1012 86 12
Cuts at: 3831 4177 1
Size: 346 449
Fragments arranged by size:
1649 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 266 915 1052 1100 1520 266
Size: 649 137 48 420 3371
Fragments arranged by size:
3371 649 420 137 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2575 2756 2919 2575
Size: 181 163 4281
Fragments arranged by size:
4281 181 163
BtsI GCAGTG_nn'
Cuts at: 947 1360 1868 947
Size: 413 508 3704
Fragments arranged by size:
3704 508 413
DraI TTT'AAA
Cuts at: 1624 1624
Size: 4625
DrdI GACnn_nn'nnGTC
Cuts at: 468 2331 3189 468
Size: 1863 858 1904
Fragments arranged by size:
1904 1863 858
EaeI y'GGCC_r
Cuts at: 218 248 263 1097 1103 1406 1550 2210 2384
Size: 30 15 834 6 303 144 660 174
Cuts at: 2384 2775 2802 4147 218
Size: 391 27 1345 696
Fragments arranged by size:
1345 834 696 660 391 303 174 144
30 27 15 6
EagI C'GGCC_G
Cuts at: 248 2210 248
Size: 1962 2663
EarI CTCTTCn'nnn_
Cuts at: 790 1453 2648 2858 790
Size: 663 1195 210 2557
Fragments arranged by size:
2557 1195 663 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2833 3143 3289 4435 2833
Size: 310 146 1146 3023
Fragments arranged by size:
3023 1146 310 146
EcoICRI GAG'CTC
Cuts at: 519 1637 519
Size: 1118 3507
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 568 1018 1054 1153 1186 1263 2450 2882
Size: 144 450 36 99 33 77 1187 432
Cuts at: 2882 3629 3897 424
Size: 747 268 1152
Fragments arranged by size:
1187 1152 747 450 432 268 144 99
77 36 33
EcoNI CCTnn'n_nnAGG
Cuts at: 295 403 775 1033 295
Size: 108 372 258 3887
Fragments arranged by size:
3887 372 258 108
EcoO109I rG'GnC_Cy
Cuts at: 352 408 780 1026 1607 1608 1764 352
Size: 56 372 246 581 1 156 3213
Fragments arranged by size:
3213 581 372 246 156 56 1
EcoRI G'AATT_C
Cuts at: 1629 1629
Size: 4625
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 801 2644 801
Size: 1843 2782
FspI TGC'GCA
Cuts at: 757 1378 1486 2406 757
Size: 621 108 920 2976
Fragments arranged by size:
2976 920 621 108
HaeII r_GCGC'y
Cuts at: 245 259 274 447 953 1142 1431 2059 2307
Size: 14 15 173 506 189 289 628 248
Cuts at: 2307 3329 4308 245
Size: 1022 979 562
Fragments arranged by size:
1022 979 628 562 506 289 248 189
173 15 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1516 1548 2467 2497 2499 2529 1516
Size: 32 919 30 2 30 3612
Fragments arranged by size:
3612 919 32 30 30 2
HincII GTy'rAC
Cuts at: 1664 1664
Size: 4625
KasI G'GCGC_C
Cuts at: 241 443 2055 2303 241
Size: 202 1612 248 2563
Fragments arranged by size:
2563 1612 248 202
KpnI G_GTAC'C
Cuts at: 1645 1645
Size: 4625
MluI A'CGCG_T
Cuts at: 4058 4058
Size: 4625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1100 3296 3480 1100
Size: 2196 184 2245
Fragments arranged by size:
2245 2196 184
MscI TGG'CCA
Cuts at: 220 1099 1408 2386 220
Size: 879 309 978 2459
Fragments arranged by size:
2459 978 879 309
MslI CAynn'nnrTG
Cuts at: 431 437 553 655 1105 1111 1459 2614 2741
Size: 6 116 102 450 6 348 1155 127
Cuts at: 2741 431
Size: 2315
Fragments arranged by size:
2315 1155 450 348 127 116 102 6
6
MspA1I CmG'CkG
Cuts at: 542 858 975 1131 1335 1434 1522 1548 1736
Size: 316 117 156 204 99 88 26 188
Cuts at: 1736 2050 2410 3423 3668 542
Size: 314 360 1013 245 1499
Fragments arranged by size:
1499 1013 360 316 314 245 204 188
156 117 99 88 26
NaeI GCC'GGC
Cuts at: 2806 2806
Size: 4625
NarI GG'CG_CC
Cuts at: 242 444 2056 2304 242
Size: 202 1612 248 2563
Fragments arranged by size:
2563 1612 248 202
NgoMIV G'CCGG_C
Cuts at: 2804 2804
Size: 4625
NspI r_CATG'y
Cuts at: 436 1083 1491 1678 2178 3085 436
Size: 647 408 187 500 907 1976
Fragments arranged by size:
1976 907 647 500 408 187
PasI CC'CwG_GG
Cuts at: 1611 1611
Size: 4625
PciI A'CATG_T
Cuts at: 3081 3081
Size: 4625
PflMI CCAn_nnn'nTGG
Cuts at: 942 1236 942
Size: 294 4331
PfoI T'CCnGG_A
Cuts at: 995 995
Size: 4625
PmeI GTTT'AAAC
Cuts at: 1624 1624
Size: 4625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4593
PpuMI rG'GwC_Cy
Cuts at: 408 408
Size: 4625
PshAI GACnn'nnGTC
Cuts at: 686 686
Size: 4625
PspOMI G'GGCC_C
Cuts at: 351 1607 351
Size: 1256 3369
PstI C_TGCA'G
Cuts at: 541 857 1672 2357 541
Size: 316 815 685 2809
Fragments arranged by size:
2809 815 685 316
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4625
PvuII CAG'CTG
Cuts at: 975 1434 1548 2050 2410 975
Size: 459 114 502 360 3190
Fragments arranged by size:
3190 502 459 360 114
RsrII CG'GwC_CG
Cuts at: 2820 2820
Size: 4625
SacI G_AGCT'C
Cuts at: 521 1639 521
Size: 1118 3507
SacII CC_GC'GG
Cuts at: 1523 1523
Size: 4625
SalI G'TCGA_C
Cuts at: 1662 1662
Size: 4625
SapI GCTCTTCn'nnn_
Cuts at: 2648 2858 2648
Size: 210 4415
SbfI CC_TGCA'GG
Cuts at: 1672 1672
Size: 4625
ScaI AGT'ACT
Cuts at: 4142 4142
Size: 4625
SexAI A'CCwGG_T
Cuts at: 374 1235 374
Size: 861 3764
SfcI C'TryA_G
Cuts at: 33 537 853 1350 1668 2353 3346 3537 33
Size: 504 316 497 318 685 993 191 1121
Fragments arranged by size:
1121 993 685 504 497 318 316 191
SfoI GGC'GCC
Cuts at: 243 445 2057 2305 243
Size: 202 1612 248 2563
Fragments arranged by size:
2563 1612 248 202
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
SmaI CCC'GGG
Cuts at: 1647 1647
Size: 4625
SmlI C'TyrA_G
Cuts at: 640 1780 2695 3187 3449 640
Size: 1140 915 492 262 1816
Fragments arranged by size:
1816 1140 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4625
SphI G_CATG'C
Cuts at: 436 1491 1678 2178 436
Size: 1055 187 500 2883
Fragments arranged by size:
2883 1055 500 187
StuI AGG'CCT
Cuts at: 1039 1039
Size: 4625
StyI C'CwwG_G
Cuts at: 509 776 1759 2113 509
Size: 267 983 354 3021
Fragments arranged by size:
3021 983 354 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 283 2208 2341 2800 3009 283
Size: 1925 133 459 209 1899
Fragments arranged by size:
1925 1899 459 209 133
TatI w'GTAC_w
Cuts at: 557 4140 557
Size: 3583 1042
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 490 522 1183 1215 1261 1293 1566 1598 4142
Size: 32 661 32 46 32 273 32 2544
Cuts at: 4142 4174 490
Size: 32 941
Fragments arranged by size:
2544 941 661 273 46 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2422 2422
Size: 4625
XbaI T'CTAG_A
Cuts at: 1656 1656
Size: 4625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 444 888 1107 1161 1257 2120 444
Size: 444 219 54 96 863 2949
Fragments arranged by size:
2949 863 444 219 96 54
XmaI C'CCGG_G
Cuts at: 1645 1645
Size: 4625
XmnI GAAnn'nnTTC
Cuts at: 343 343
Size: 4625
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AloI AscI AvrII BaeI BbvCI BfrBI
BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PmlI PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI