Restriction map for
pF1KB5906
(Circular) (Six-base) MAPSORT of: pF1KB5906.seq Check: 6134 from: 1 to: 4691
pF1KB5906 4691 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1729 1729
Size: 4691
Acc65I G'GTAC_C
Cuts at: 1707 1707
Size: 4691
AclI AA'CG_TT
Cuts at: 4531 4531
Size: 4691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 1126 1127 2516 2948 3695 3963 233
Size: 893 1 1389 432 747 268 961
Fragments arranged by size:
1389 961 893 747 432 268 1
AflIII A'CryG_T
Cuts at: 2043 3147 4124 2043
Size: 1104 977 2610
Fragments arranged by size:
2610 1104 977
AgeI A'CCGG_T
Cuts at: 3079 3079
Size: 4691
AlwNI CAG_nnn'CTG
Cuts at: 1996 3563 1996
Size: 1567 3124
ApaLI G'TGCA_C
Cuts at: 3461 3461
Size: 4691
ApoI r'AATT_y
Cuts at: 898 1180 1695 4044 4300 898
Size: 282 515 2349 256 1289
Fragments arranged by size:
2349 1289 515 282 256
AseI AT'TA_AT
Cuts at: 20 1073 20
Size: 1053 3638
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4691
AvaI C'yCGr_G
Cuts at: 169 435 911 1711 169
Size: 266 476 800 3149
Fragments arranged by size:
3149 800 476 266
BamHI G'GATC_C
Cuts at: 1716 1716
Size: 4691
BanI G'GyrC_C
Cuts at: 1707 2121 2369 2404 1707
Size: 414 248 35 3994
Fragments arranged by size:
3994 414 248 35
BanII G_rGCy'C
Cuts at: 367 1705 2735 367
Size: 1338 1030 2323
Fragments arranged by size:
2323 1338 1030
BbeI G_GCGC'C
Cuts at: 2125 2373 2125
Size: 248 4443
BciVI GTATCCnnnnn_n'
Cuts at: 2589 3350 4691 2589
Size: 761 1341 2589
Fragments arranged by size:
2589 1341 761
BclI T'GATC_A
Cuts at: 2211 2211
Size: 4691
BglI GCCn_nnn'nGGC
Cuts at: 854 1292 854
Size: 438 4253
BglII A'GATC_T
Cuts at: 2206 4243 2206
Size: 2037 2654
BlpI GC'TnA_GC
Cuts at: 1803 1803
Size: 4691
Bme1580I G_kGCm'C
Cuts at: 2316 2409 3465 2316
Size: 93 1056 3542
Fragments arranged by size:
3542 1056 93
BmgBI CAC'GTC
Cuts at: 928 928
Size: 4691
BmrI ACTGGGnnnn_n'
Cuts at: 1334 1903 1984 2319 4463 1334
Size: 569 81 335 2144 1562
Fragments arranged by size:
2144 1562 569 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 4691
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1867 2746 3238 3536 1867
Size: 879 492 298 3022
Fragments arranged by size:
3022 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1128 1209 4395 1128
Size: 81 3186 1424
Fragments arranged by size:
3186 1424 81
BsaAI yAC'GTr
Cuts at: 2044 2044
Size: 4691
BsaBI GATnn'nnATC
Cuts at: 6 2205 4242 6
Size: 2199 2037 455
Fragments arranged by size:
2199 2037 455
BsaHI Gr'CG_yC
Cuts at: 2122 2370 3072 4568 2122
Size: 248 702 1496 2245
Fragments arranged by size:
2245 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1186 1880 2401 3079 3353 3500 3911 1186
Size: 694 521 678 274 147 411 1966
Fragments arranged by size:
1966 694 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1666 1696 1666
Size: 30 4661
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1286 1343 1550 1286
Size: 57 207 4427
Fragments arranged by size:
4427 207 57
BseYI C'CCAG_C
Cuts at: 164 185 424 2116 2874 3451 3947 164
Size: 21 239 1692 758 577 496 908
Fragments arranged by size:
1692 908 758 577 496 239 21
BsiEI CG_ry'CG
Cuts at: 104 2233 2279 3487 104
Size: 2129 46 1208 1308
Fragments arranged by size:
2129 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 367 1658 1705 2483 2673 3465 367
Size: 1291 47 778 190 792 1593
Fragments arranged by size:
1593 1291 792 778 190 47
BsmI GAATG_Cn'
Cuts at: 1441 1441
Size: 4691
Bsp1286I G_dGCh'C
Cuts at: 367 1658 1705 2316 2409 2483 2673 2735 3465
Size: 1291 47 611 93 74 190 62 730
Cuts at: 3465 367
Size: 1593
Fragments arranged by size:
1593 1291 730 611 190 93 74 62
47
BspEI T'CCGG_A
Cuts at: 1186 3911 1186
Size: 2725 1966
BspHI T'CATG_A
Cuts at: 930 3867 930
Size: 2937 1754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1741 1955 2257 2638 1741
Size: 214 302 381 3794
Fragments arranged by size:
3794 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2983 3037 4521 4686 50
Size: 2933 54 1484 165 55
Fragments arranged by size:
2933 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1254 2603 1254
Size: 1349 3342
BsrFI r'CCGG_y
Cuts at: 2689 2870 3079 2689
Size: 181 209 4301
Fragments arranged by size:
4301 209 181
BsrGI T'GTAC_A
Cuts at: 1079 1079
Size: 4691
BssSI C'ACGA_G
Cuts at: 2962 3320 2962
Size: 358 4333
BstAPI GCAn_nnn'nTGC
Cuts at: 2068 2068
Size: 4691
BstBI TT'CG_AA
Cuts at: 3052 3052
Size: 4691
BstXI CCAn_nnnn'nTGG
Cuts at: 148 331 709 148
Size: 183 378 4130
Fragments arranged by size:
4130 378 183
BstYI r'GATC_y
Cuts at: 1 1716 2183 2206 2541 2787 3799 3885 3897
Size: 1715 467 23 335 246 1012 86 12
Cuts at: 3897 4243 1
Size: 346 449
Fragments arranged by size:
1715 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 563 563
Size: 4691
BtgI C'CryG_G
Cuts at: 107 239 744 753 849 882 1280 1287 1566
Size: 132 505 9 96 33 398 7 279
Cuts at: 1566 1578 107
Size: 12 3220
Fragments arranged by size:
3220 505 398 279 132 96 33 12
9 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2641 2822 2985 2641
Size: 181 163 4347
Fragments arranged by size:
4347 181 163
BtsI GCAGTG_nn'
Cuts at: 606 681 774 819 1317 1934 606
Size: 75 93 45 498 617 3363
Fragments arranged by size:
3363 617 498 93 75 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1236 1271 1236
Size: 35 4656
DraI TTT'AAA
Cuts at: 1690 1690
Size: 4691
DrdI GACnn_nn'nnGTC
Cuts at: 2397 3255 2397
Size: 858 3833
EaeI y'GGCC_r
Cuts at: 258 282 408 639 846 1323 2276 2450 2841
Size: 24 126 231 207 477 953 174 391
Cuts at: 2841 2868 4213 258
Size: 27 1345 736
Fragments arranged by size:
1345 953 736 477 391 231 207 174
126 27 24
EagI C'GGCC_G
Cuts at: 2276 2276
Size: 4691
EarI CTCTTCn'nnn_
Cuts at: 2714 2924 2714
Size: 210 4481
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 887 2899 3209 3355 4501 887
Size: 2012 310 146 1146 1077
Fragments arranged by size:
2012 1146 1077 310 146
EcoICRI GAG'CTC
Cuts at: 365 1703 365
Size: 1338 3353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 1126 1127 2516 2948 3695 3963 233
Size: 893 1 1389 432 747 268 961
Fragments arranged by size:
1389 961 893 747 432 268 1
EcoO109I rG'GnC_Cy
Cuts at: 152 1276 1452 1830 152
Size: 1124 176 378 3013
Fragments arranged by size:
3013 1124 378 176
EcoRI G'AATT_C
Cuts at: 1180 1695 1180
Size: 515 4176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 4691
FspI TGC'GCA
Cuts at: 2472 2472
Size: 4691
HaeII r_GCGC'y
Cuts at: 2125 2373 3395 4374 2125
Size: 248 1022 979 2442
Fragments arranged by size:
2442 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 637 669 907 939 2533 2563 2565 2595 637
Size: 32 238 32 1594 30 2 30 2733
Fragments arranged by size:
2733 1594 238 32 32 30 30 2
HincII GTy'rAC
Cuts at: 317 1730 317
Size: 1413 3278
KasI G'GCGC_C
Cuts at: 2121 2369 2121
Size: 248 4443
KpnI G_GTAC'C
Cuts at: 1711 1711
Size: 4691
MfeI C'AATT_G
Cuts at: 998 998
Size: 4691
MluI A'CGCG_T
Cuts at: 4124 4124
Size: 4691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3362 3546 3362
Size: 184 4507
MscI TGG'CCA
Cuts at: 260 284 410 641 2452 260
Size: 24 126 231 1811 2499
Fragments arranged by size:
2499 1811 231 126 24
MslI CAynn'nnrTG
Cuts at: 970 2680 2807 970
Size: 1710 127 2854
Fragments arranged by size:
2854 1710 127
MspA1I CmG'CkG
Cuts at: 722 755 1568 1802 2116 2476 3489 3734 722
Size: 33 813 234 314 360 1013 245 1679
Fragments arranged by size:
1679 1013 813 360 314 245 234 33
NaeI GCC'GGC
Cuts at: 2872 2872
Size: 4691
NarI GG'CG_CC
Cuts at: 2122 2370 2122
Size: 248 4443
NcoI C'CATG_G
Cuts at: 107 1280 107
Size: 1173 3518
NgoMIV G'CCGG_C
Cuts at: 2870 2870
Size: 4691
NspI r_CATG'y
Cuts at: 1744 2244 3151 1744
Size: 500 907 3284
Fragments arranged by size:
3284 907 500
PasI CC'CwG_GG
Cuts at: 324 324
Size: 4691
PciI A'CATG_T
Cuts at: 3147 3147
Size: 4691
PmeI GTTT'AAAC
Cuts at: 1690 1690
Size: 4691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 4659
PpuMI rG'GwC_Cy
Cuts at: 1276 1276
Size: 4691
PstI C_TGCA'G
Cuts at: 1738 2423 1738
Size: 685 4006
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4691
PvuII CAG'CTG
Cuts at: 2116 2476 2116
Size: 360 4331
RsrII CG'GwC_CG
Cuts at: 2886 2886
Size: 4691
SacI G_AGCT'C
Cuts at: 367 1705 367
Size: 1338 3353
SacII CC_GC'GG
Cuts at: 756 1569 756
Size: 813 3878
SalI G'TCGA_C
Cuts at: 1728 1728
Size: 4691
SapI GCTCTTCn'nnn_
Cuts at: 2714 2924 2714
Size: 210 4481
SbfI CC_TGCA'GG
Cuts at: 1738 1738
Size: 4691
ScaI AGT'ACT
Cuts at: 4208 4208
Size: 4691
SexAI A'CCwGG_T
Cuts at: 1482 1482
Size: 4691
SfcI C'TryA_G
Cuts at: 33 495 1734 2419 3412 3603 33
Size: 462 1239 685 993 191 1121
Fragments arranged by size:
1239 1121 993 685 462 191
SfoI GGC'GCC
Cuts at: 2123 2371 2123
Size: 248 4443
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4691
SmaI CCC'GGG
Cuts at: 171 1713 171
Size: 1542 3149
SmlI C'TyrA_G
Cuts at: 1846 2761 3253 3515 1846
Size: 915 492 262 3022
Fragments arranged by size:
3022 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4691
SphI G_CATG'C
Cuts at: 1744 2244 1744
Size: 500 4191
SrfI GCCC'GGGC
Cuts at: 171 171
Size: 4691
StuI AGG'CCT
Cuts at: 980 980
Size: 4691
StyI C'CwwG_G
Cuts at: 107 918 1280 1825 2179 107
Size: 811 362 545 354 2619
Fragments arranged by size:
2619 811 545 362 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 178 973 2274 2407 2866 3075 178
Size: 795 1301 133 459 209 1794
Fragments arranged by size:
1794 1301 795 459 209 133
TatI w'GTAC_w
Cuts at: 587 1079 4206 587
Size: 492 3127 1072
Fragments arranged by size:
3127 1072 492
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4208 4240 4208
Size: 32 4659
Tth111I GACn'n_nGTC
Cuts at: 2488 2488
Size: 4691
XbaI T'CTAG_A
Cuts at: 1722 1722
Size: 4691
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2186 2186
Size: 4691
XmaI C'CCGG_G
Cuts at: 169 1711 169
Size: 1542 3149
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmtI BpmI Bpu10I BsgI BsiWI BsmBI BssHII BstEII
BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SanDI
SfiI SgrAI SnaBI SspI SwaI XhoI XmnI ZraI