Restriction map for
pF1KB5905
(Circular) (Six-base) MAPSORT of: pF1KB5905.seq Check: 3114 from: 1 to: 4619
pF1KB5905 4619 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1657 1657
Size: 4619
Acc65I G'GTAC_C
Cuts at: 1635 1635
Size: 4619
AclI AA'CG_TT
Cuts at: 4459 4459
Size: 4619
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1033 2444 2876 3623 3891 1000
Size: 33 1411 432 747 268 1728
Fragments arranged by size:
1728 1411 747 432 268 33
AflIII A'CryG_T
Cuts at: 1304 1971 3075 4052 1304
Size: 667 1104 977 1871
Fragments arranged by size:
1871 1104 977 667
AgeI A'CCGG_T
Cuts at: 1472 3007 1472
Size: 1535 3084
AlwNI CAG_nnn'CTG
Cuts at: 1924 3491 1924
Size: 1567 3052
ApaLI G'TGCA_C
Cuts at: 3389 3389
Size: 4619
ApoI r'AATT_y
Cuts at: 1174 1623 3972 4228 1174
Size: 449 2349 256 1565
Fragments arranged by size:
2349 1565 449 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4619
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
AvaI C'yCGr_G
Cuts at: 1217 1639 1217
Size: 422 4197
AvrII C'CTAG_G
Cuts at: 321 321
Size: 4619
BamHI G'GATC_C
Cuts at: 1644 1644
Size: 4619
BanI G'GyrC_C
Cuts at: 142 1225 1635 2049 2297 2332 142
Size: 1083 410 414 248 35 2429
Fragments arranged by size:
2429 1083 414 410 248 35
BanII G_rGCy'C
Cuts at: 664 1533 1633 2663 664
Size: 869 100 1030 2620
Fragments arranged by size:
2620 1030 869 100
BbeI G_GCGC'C
Cuts at: 2053 2301 2053
Size: 248 4371
BbsI GAAGACnn'nnnn_
Cuts at: 233 233
Size: 4619
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4585
BciVI GTATCCnnnnn_n'
Cuts at: 2517 3278 4619 2517
Size: 761 1341 2517
Fragments arranged by size:
2517 1341 761
BclI T'GATC_A
Cuts at: 2139 2139
Size: 4619
BglII A'GATC_T
Cuts at: 1101 2134 4171 1101
Size: 1033 2037 1549
Fragments arranged by size:
2037 1549 1033
BlpI GC'TnA_GC
Cuts at: 710 1731 710
Size: 1021 3598
Bme1580I G_kGCm'C
Cuts at: 145 1403 2244 2337 3393 145
Size: 1258 841 93 1056 1371
Fragments arranged by size:
1371 1258 1056 841 93
BmrI ACTGGGnnnn_n'
Cuts at: 921 1397 1831 1912 2247 4391 921
Size: 476 434 81 335 2144 1149
Fragments arranged by size:
2144 1149 476 434 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 306 2986 306
Size: 2680 1939
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 644 1229 644
Size: 585 4034
Bpu10I CC'TnA_GC
Cuts at: 663 663
Size: 4619
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1058 1795 2674 3166 3464 1058
Size: 737 879 492 298 2213
Fragments arranged by size:
2213 879 737 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4323 4323
Size: 4619
BsaAI yAC'GTr
Cuts at: 1972 1972
Size: 4619
BsaBI GATnn'nnATC
Cuts at: 6 1350 2133 4170 6
Size: 1344 783 2037 455
Fragments arranged by size:
2037 1344 783 455
BsaHI Gr'CG_yC
Cuts at: 2050 2298 3000 4496 2050
Size: 248 702 1496 2173
Fragments arranged by size:
2173 1496 702 248
BsaWI w'CCGG_w
Cuts at: 551 1472 1808 2329 3007 3281 3428 3839 551
Size: 921 336 521 678 274 147 411 1331
Fragments arranged by size:
1331 921 678 521 411 336 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 423 453 1349 1379 1423 1453 423
Size: 30 896 30 44 30 3589
Fragments arranged by size:
3589 896 44 30 30 30
BseYI C'CCAG_C
Cuts at: 1526 2044 2802 3379 3875 1526
Size: 518 758 577 496 2270
Fragments arranged by size:
2270 758 577 518 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 378 143
Size: 235 4384
BsiEI CG_ry'CG
Cuts at: 104 2161 2207 3415 104
Size: 2057 46 1208 1308
Fragments arranged by size:
2057 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 664 1633 2411 2601 3393 664
Size: 969 778 190 792 1890
Fragments arranged by size:
1890 969 792 778 190
BsmI GAATG_Cn'
Cuts at: 394 1528 394
Size: 1134 3485
Bsp1286I G_dGCh'C
Cuts at: 145 664 1403 1533 1633 2244 2337 2411 2601
Size: 519 739 130 100 611 93 74 190
Cuts at: 2601 2663 3393 145
Size: 62 730 1371
Fragments arranged by size:
1371 739 730 611 519 190 130 100
93 74 62
BspEI T'CCGG_A
Cuts at: 3839 3839
Size: 4619
BspHI T'CATG_A
Cuts at: 951 1389 1514 3795 951
Size: 438 125 2281 1775
Fragments arranged by size:
2281 1775 438 125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1669 1883 2185 2566 1669
Size: 214 302 381 3722
Fragments arranged by size:
3722 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2911 2965 4449 4614 50
Size: 2861 54 1484 165 55
Fragments arranged by size:
2861 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 890 2531 890
Size: 1641 2978
BsrFI r'CCGG_y
Cuts at: 1472 2617 2798 3007 1472
Size: 1145 181 209 3084
Fragments arranged by size:
3084 1145 209 181
BssSI C'ACGA_G
Cuts at: 2890 3248 2890
Size: 358 4261
BstAPI GCAn_nnn'nTGC
Cuts at: 1996 1996
Size: 4619
BstBI TT'CG_AA
Cuts at: 2980 2980
Size: 4619
BstXI CCAn_nnnn'nTGG
Cuts at: 1475 1475
Size: 4619
BstYI r'GATC_y
Cuts at: 1 1101 1644 2111 2134 2469 2715 3727 3813
Size: 1100 543 467 23 335 246 1012 86
Cuts at: 3813 3825 4171 1
Size: 12 346 449
Fragments arranged by size:
1100 1012 543 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2569 2750 2913 2569
Size: 181 163 4275
Fragments arranged by size:
4275 181 163
BtsI GCAGTG_nn'
Cuts at: 433 1488 1862 433
Size: 1055 374 3190
Fragments arranged by size:
3190 1055 374
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 199 234 199
Size: 35 4584
DraI TTT'AAA
Cuts at: 1618 1618
Size: 4619
DrdI GACnn_nn'nnGTC
Cuts at: 2325 3183 2325
Size: 858 3761
EaeI y'GGCC_r
Cuts at: 2204 2378 2769 2796 4141 2204
Size: 174 391 27 1345 2682
Fragments arranged by size:
2682 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2204 2204
Size: 4619
EarI CTCTTCn'nnn_
Cuts at: 275 1032 2642 2852 275
Size: 757 1610 210 2042
Fragments arranged by size:
2042 1610 757 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2827 3137 3283 4429 2827
Size: 310 146 1146 3017
Fragments arranged by size:
3017 1146 310 146
EcoICRI GAG'CTC
Cuts at: 662 1631 662
Size: 969 3650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 644 1000 1033 1229 2444 2876 3623 3891 644
Size: 356 33 196 1215 432 747 268 1372
Fragments arranged by size:
1372 1215 747 432 356 268 196 33
EcoNI CCTnn'n_nnAGG
Cuts at: 1330 1330
Size: 4619
EcoO109I rG'GnC_Cy
Cuts at: 917 1758 917
Size: 841 3778
EcoRI G'AATT_C
Cuts at: 1623 1623
Size: 4619
EcoRV GAT'ATC
Cuts at: 903 903
Size: 4619
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 4619
FspI TGC'GCA
Cuts at: 2400 2400
Size: 4619
HaeII r_GCGC'y
Cuts at: 2053 2301 3323 4302 2053
Size: 248 1022 979 2370
Fragments arranged by size:
2370 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 665 697 988 1020 2461 2491 2493 2523 665
Size: 32 291 32 1441 30 2 30 2761
Fragments arranged by size:
2761 1441 291 32 32 30 30 2
HincII GTy'rAC
Cuts at: 237 1200 1658 237
Size: 963 458 3198
Fragments arranged by size:
3198 963 458
KasI G'GCGC_C
Cuts at: 2049 2297 2049
Size: 248 4371
KpnI G_GTAC'C
Cuts at: 1639 1639
Size: 4619
MfeI C'AATT_G
Cuts at: 383 1088 383
Size: 705 3914
MluI A'CGCG_T
Cuts at: 4052 4052
Size: 4619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1235 3290 3474 1235
Size: 2055 184 2380
Fragments arranged by size:
2380 2055 184
MscI TGG'CCA
Cuts at: 2380 2380
Size: 4619
MslI CAynn'nnrTG
Cuts at: 956 1123 2608 2735 956
Size: 167 1485 127 2840
Fragments arranged by size:
2840 1485 167 127
MspA1I CmG'CkG
Cuts at: 827 1730 2044 2404 3417 3662 827
Size: 903 314 360 1013 245 1784
Fragments arranged by size:
1784 1013 903 360 314 245
NaeI GCC'GGC
Cuts at: 2800 2800
Size: 4619
NarI GG'CG_CC
Cuts at: 2050 2298 2050
Size: 248 4371
NgoMIV G'CCGG_C
Cuts at: 2798 2798
Size: 4619
NspI r_CATG'y
Cuts at: 1308 1672 2172 3079 1308
Size: 364 500 907 2848
Fragments arranged by size:
2848 907 500 364
PciI A'CATG_T
Cuts at: 1304 3075 1304
Size: 1771 2848
PfoI T'CCnGG_A
Cuts at: 621 919 621
Size: 298 4321
PmeI GTTT'AAAC
Cuts at: 1618 1618
Size: 4619
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 3758
Size: 32 4587
PpuMI rG'GwC_Cy
Cuts at: 917 917
Size: 4619
PspXI vC'TCGA_Gb
Cuts at: 1217 1217
Size: 4619
PstI C_TGCA'G
Cuts at: 900 1666 2351 900
Size: 766 685 3168
Fragments arranged by size:
3168 766 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4619
PvuII CAG'CTG
Cuts at: 827 2044 2404 827
Size: 1217 360 3042
Fragments arranged by size:
3042 1217 360
RsrII CG'GwC_CG
Cuts at: 2814 2814
Size: 4619
SacI G_AGCT'C
Cuts at: 664 1633 664
Size: 969 3650
SalI G'TCGA_C
Cuts at: 1656 1656
Size: 4619
SapI GCTCTTCn'nnn_
Cuts at: 2642 2852 2642
Size: 210 4409
SbfI CC_TGCA'GG
Cuts at: 1666 1666
Size: 4619
ScaI AGT'ACT
Cuts at: 1071 4136 1071
Size: 3065 1554
SfcI C'TryA_G
Cuts at: 33 510 704 896 1662 2347 3340 3531 33
Size: 477 194 192 766 685 993 191 1121
Fragments arranged by size:
1121 993 766 685 477 194 192 191
SfoI GGC'GCC
Cuts at: 2051 2299 2051
Size: 248 4371
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4619
SmaI CCC'GGG
Cuts at: 1641 1641
Size: 4619
SmlI C'TyrA_G
Cuts at: 1073 1217 1774 2689 3181 3443 1073
Size: 144 557 915 492 262 2249
Fragments arranged by size:
2249 915 557 492 262 144
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4619
SphI G_CATG'C
Cuts at: 1672 2172 1672
Size: 500 4119
SspI AAT'ATT
Cuts at: 243 243
Size: 4619
StuI AGG'CCT
Cuts at: 680 680
Size: 4619
StyI C'CwwG_G
Cuts at: 321 1753 2107 321
Size: 1432 354 2833
Fragments arranged by size:
2833 1432 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2202 2335 2794 3003 2202
Size: 133 459 209 3818
Fragments arranged by size:
3818 459 209 133
TatI w'GTAC_w
Cuts at: 166 1069 4134 166
Size: 903 3065 651
Fragments arranged by size:
3065 903 651
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 168 200 348 380 1349 1381 1423 1455 4136
Size: 32 148 32 969 32 42 32 2681
Cuts at: 4136 4168 168
Size: 32 619
Fragments arranged by size:
2681 969 619 148 42 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 232 915 2416 232
Size: 683 1501 2435
Fragments arranged by size:
2435 1501 683
XbaI T'CTAG_A
Cuts at: 1650 1650
Size: 4619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2114 2114
Size: 4619
XhoI C'TCGA_G
Cuts at: 1217 1217
Size: 4619
XmaI C'CCGG_G
Cuts at: 1639 1639
Size: 4619
XmnI GAAnn'nnTTC
Cuts at: 802 802
Size: 4619
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BfrBI BglI BmgBI BmtI
BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI DraIII FseI FspAI HindIII HpaI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI ZraI