Restriction map for
pF1KB5902
(Circular) (Six-base) MAPSORT of: pF1KB5902.seq Check: 7624 from: 1 to: 4688
pF1KB5902 4688 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1111 1111
Size: 4688
AccI GT'mk_AC
Cuts at: 1726 1726
Size: 4688
Acc65I G'GTAC_C
Cuts at: 1704 1704
Size: 4688
AclI AA'CG_TT
Cuts at: 4528 4528
Size: 4688
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 663 2513 2945 3692 3960 663
Size: 1850 432 747 268 1391
Fragments arranged by size:
1850 1391 747 432 268
AflIII A'CryG_T
Cuts at: 2040 3144 4121 2040
Size: 1104 977 2607
Fragments arranged by size:
2607 1104 977
AgeI A'CCGG_T
Cuts at: 3076 3076
Size: 4688
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1151 1151
Size: 4688
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 106 138 106
Size: 32 4656
AlwNI CAG_nnn'CTG
Cuts at: 126 187 703 1231 1993 3560 126
Size: 61 516 528 762 1567 1254
Fragments arranged by size:
1567 1254 762 528 516 61
ApaI G_GGCC'C
Cuts at: 210 389 821 210
Size: 179 432 4077
Fragments arranged by size:
4077 432 179
ApaLI G'TGCA_C
Cuts at: 559 3458 559
Size: 2899 1789
ApoI r'AATT_y
Cuts at: 1692 4041 4297 1692
Size: 2349 256 2083
Fragments arranged by size:
2349 2083 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4688
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4688
AvaI C'yCGr_G
Cuts at: 1654 1660 1666 1708 1654
Size: 6 6 42 4634
Fragments arranged by size:
4634 42 6 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1211 1244 1211
Size: 33 4655
BamHI G'GATC_C
Cuts at: 1192 1713 1192
Size: 521 4167
BanI G'GyrC_C
Cuts at: 369 1408 1435 1704 2118 2366 2401 369
Size: 1039 27 269 414 248 35 2656
Fragments arranged by size:
2656 1039 414 269 248 35 27
BanII G_rGCy'C
Cuts at: 210 222 389 821 899 1258 1431 1655 1661
Size: 12 167 432 78 359 173 224 6
Cuts at: 1661 1667 1702 2732 210
Size: 6 35 1030 2166
Fragments arranged by size:
2166 1030 432 359 224 173 167 78
35 12 6 6
BbeI G_GCGC'C
Cuts at: 373 2122 2370 373
Size: 1749 248 2691
Fragments arranged by size:
2691 1749 248
BbsI GAAGACnn'nnnn_
Cuts at: 1539 1539
Size: 4688
BbvCI CC'TCA_GC
Cuts at: 188 188
Size: 4688
BciVI GTATCCnnnnn_n'
Cuts at: 2586 3347 4688 2586
Size: 761 1341 2586
Fragments arranged by size:
2586 1341 761
BclI T'GATC_A
Cuts at: 2208 2208
Size: 4688
BglII A'GATC_T
Cuts at: 2203 4240 2203
Size: 2037 2651
BlpI GC'TnA_GC
Cuts at: 412 1800 412
Size: 1388 3300
Bme1580I G_kGCm'C
Cuts at: 210 389 563 821 1413 1440 2313 2406 3462
Size: 179 174 258 592 27 873 93 1056
Cuts at: 3462 210
Size: 1436
Fragments arranged by size:
1436 1056 873 592 258 179 174 93
27
BmrI ACTGGGnnnn_n'
Cuts at: 666 1900 1981 2316 4460 666
Size: 1234 81 335 2144 894
Fragments arranged by size:
2144 1234 894 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3055 3055
Size: 4688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 160 241 913 944 1058 1208 1321 1526
Size: 14 81 672 31 114 150 113 205
Cuts at: 1526 1633 146
Size: 107 3201
Fragments arranged by size:
3201 672 205 150 114 113 107 81
31 14
Bpu10I CC'TnA_GC
Cuts at: 188 188
Size: 4688
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1864 2743 3235 3533 1864
Size: 879 492 298 3019
Fragments arranged by size:
3019 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4392 4392
Size: 4688
BsaAI yAC'GTr
Cuts at: 2041 2041
Size: 4688
BsaBI GATnn'nnATC
Cuts at: 6 2202 4239 6
Size: 2196 2037 455
Fragments arranged by size:
2196 2037 455
BsaHI Gr'CG_yC
Cuts at: 370 536 2119 2367 3069 4565 370
Size: 166 1583 248 702 1496 493
Fragments arranged by size:
1583 1496 702 493 248 166
BsaWI w'CCGG_w
Cuts at: 716 1623 1647 1877 2398 3076 3350 3497 3908
Size: 907 24 230 521 678 274 147 411
Cuts at: 3908 716
Size: 1496
Fragments arranged by size:
1496 907 678 521 411 274 230 147
24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 106 136 1152 1182 1364 1394 106
Size: 30 1016 30 182 30 3400
Fragments arranged by size:
3400 1016 182 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 366 995 1293 1621 366
Size: 629 298 328 3433
Fragments arranged by size:
3433 629 328 298
BseYI C'CCAG_C
Cuts at: 227 519 696 1090 1316 1328 1443 2113 2871
Size: 292 177 394 226 12 115 670 758
Cuts at: 2871 3448 3944 227
Size: 577 496 971
Fragments arranged by size:
971 758 670 577 496 394 292 226
177 115 12
BsiEI CG_ry'CG
Cuts at: 104 486 2230 2276 3484 104
Size: 382 1744 46 1208 1308
Fragments arranged by size:
1744 1308 1208 382 46
BsiHKAI G_wGCw'C
Cuts at: 563 1258 1702 2480 2670 3462 563
Size: 695 444 778 190 792 1789
Fragments arranged by size:
1789 792 778 695 444 190
BsmBI CGTCTCn'nnnn_
Cuts at: 864 864
Size: 4688
Bsp1286I G_dGCh'C
Cuts at: 210 222 389 563 821 899 1258 1413 1431
Size: 12 167 174 258 78 359 155 18
Cuts at: 1431 1440 1655 1661 1667 1702 2313 2406 2480
Size: 9 215 6 6 35 611 93 74
Cuts at: 2480 2670 2732 3462 210
Size: 190 62 730 1436
Fragments arranged by size:
1436 730 611 359 258 215 190 174
167 155 93 78 74 62 35 18
12 9 6 6
BspEI T'CCGG_A
Cuts at: 716 3908 716
Size: 3192 1496
BspHI T'CATG_A
Cuts at: 3864 3864
Size: 4688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 919 1111 1738 1952 2254 2635 919
Size: 192 627 214 302 381 2972
Fragments arranged by size:
2972 627 381 302 214 192
BsrBI CCG'CTC
Cuts at: 50 2980 3034 4518 4683 50
Size: 2930 54 1484 165 55
Fragments arranged by size:
2930 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1345 2600 1345
Size: 1255 3433
BsrFI r'CCGG_y
Cuts at: 485 538 2686 2867 3076 485
Size: 53 2148 181 209 2097
Fragments arranged by size:
2148 2097 209 181 53
BssHII G'CGCG_C
Cuts at: 528 528
Size: 4688
BssSI C'ACGA_G
Cuts at: 2959 3317 2959
Size: 358 4330
BstAPI GCAn_nnn'nTGC
Cuts at: 2065 2065
Size: 4688
BstBI TT'CG_AA
Cuts at: 3049 3049
Size: 4688
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4688
BstXI CCAn_nnnn'nTGG
Cuts at: 1420 1420
Size: 4688
BstYI r'GATC_y
Cuts at: 1 261 637 1192 1713 2180 2203 2538 2784
Size: 260 376 555 521 467 23 335 246
Cuts at: 2784 3796 3882 3894 4240 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 555 521 467 449 376 346 335
260 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 565 565
Size: 4688
BtgI C'CryG_G
Cuts at: 554 806 554
Size: 252 4436
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2638 2819 2982 2638
Size: 181 163 4344
Fragments arranged by size:
4344 181 163
BtsI GCAGTG_nn'
Cuts at: 989 1385 1931 989
Size: 396 546 3746
Fragments arranged by size:
3746 546 396
DraI TTT'AAA
Cuts at: 1687 1687
Size: 4688
DrdI GACnn_nn'nnGTC
Cuts at: 2394 3252 2394
Size: 858 3830
EaeI y'GGCC_r
Cuts at: 336 483 2273 2447 2838 2865 4210 336
Size: 147 1790 174 391 27 1345 814
Fragments arranged by size:
1790 1345 814 391 174 147 27
EagI C'GGCC_G
Cuts at: 483 2273 483
Size: 1790 2898
EarI CTCTTCn'nnn_
Cuts at: 273 790 2711 2921 273
Size: 517 1921 210 2040
Fragments arranged by size:
2040 1921 517 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2896 3206 3352 4498 2896
Size: 310 146 1146 3086
Fragments arranged by size:
3086 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1256 1700 1256
Size: 444 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 160 241 663 913 944 1058 1208 1321
Size: 14 81 422 250 31 114 150 113
Cuts at: 1321 1526 1633 2513 2945 3692 3960 146
Size: 205 107 880 432 747 268 874
Fragments arranged by size:
880 874 747 432 422 268 250 205
150 114 113 107 81 31 14
EcoNI CCTnn'n_nnAGG
Cuts at: 607 1013 607
Size: 406 4282
EcoO109I rG'GnC_Cy
Cuts at: 192 385 392 421 818 974 1018 1094 1291
Size: 193 7 29 397 156 44 76 197
Cuts at: 1291 1827 192
Size: 536 3053
Fragments arranged by size:
3053 536 397 197 193 156 76 44
29 7
EcoRI G'AATT_C
Cuts at: 1692 1692
Size: 4688
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2707 2707
Size: 4688
FspI TGC'GCA
Cuts at: 436 2469 436
Size: 2033 2655
HaeII r_GCGC'y
Cuts at: 373 2122 2370 3392 4371 373
Size: 1749 248 1022 979 690
Fragments arranged by size:
1749 1022 979 690 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 272 304 405 437 728 760 1255 1287 1566
Size: 32 101 32 291 32 495 32 279
Cuts at: 1566 1598 2530 2560 2562 2592 272
Size: 32 932 30 2 30 2368
Fragments arranged by size:
2368 932 495 291 279 101 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1727 1727
Size: 4688
HindIII A'AGCT_T
Cuts at: 777 777
Size: 4688
KasI G'GCGC_C
Cuts at: 369 2118 2366 369
Size: 1749 248 2691
Fragments arranged by size:
2691 1749 248
KpnI G_GTAC'C
Cuts at: 1708 1708
Size: 4688
MluI A'CGCG_T
Cuts at: 4121 4121
Size: 4688
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 677 3359 3543 677
Size: 2682 184 1822
Fragments arranged by size:
2682 1822 184
MscI TGG'CCA
Cuts at: 2449 2449
Size: 4688
MslI CAynn'nnrTG
Cuts at: 1162 2677 2804 1162
Size: 1515 127 3046
Fragments arranged by size:
3046 1515 127
MspA1I CmG'CkG
Cuts at: 325 440 471 556 1347 1799 2113 2473 3486
Size: 115 31 85 791 452 314 360 1013
Cuts at: 3486 3731 325
Size: 245 1282
Fragments arranged by size:
1282 1013 791 452 360 314 245 115
85 31
NaeI GCC'GGC
Cuts at: 487 540 2869 487
Size: 53 2329 2306
Fragments arranged by size:
2329 2306 53
NarI GG'CG_CC
Cuts at: 370 2119 2367 370
Size: 1749 248 2691
Fragments arranged by size:
2691 1749 248
NcoI C'CATG_G
Cuts at: 806 806
Size: 4688
NgoMIV G'CCGG_C
Cuts at: 485 538 2867 485
Size: 53 2329 2306
Fragments arranged by size:
2329 2306 53
NspI r_CATG'y
Cuts at: 1741 2241 3148 1741
Size: 500 907 3281
Fragments arranged by size:
3281 907 500
PasI CC'CwG_GG
Cuts at: 821 1197 821
Size: 376 4312
PciI A'CATG_T
Cuts at: 3144 3144
Size: 4688
PflMI CCAn_nnn'nTGG
Cuts at: 313 345 1231 313
Size: 32 886 3770
Fragments arranged by size:
3770 886 32
PfoI T'CCnGG_A
Cuts at: 257 423 746 257
Size: 166 323 4199
Fragments arranged by size:
4199 323 166
PmeI GTTT'AAAC
Cuts at: 1687 1687
Size: 4688
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 106 138 1115 1147 3827 3859 106
Size: 32 977 32 2680 32 935
Fragments arranged by size:
2680 977 935 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 192 421 974 1094 1291 192
Size: 229 553 120 197 3589
Fragments arranged by size:
3589 553 229 197 120
PspOMI G'GGCC_C
Cuts at: 206 385 817 206
Size: 179 432 4077
Fragments arranged by size:
4077 432 179
PstI C_TGCA'G
Cuts at: 146 241 458 1121 1735 2420 146
Size: 95 217 663 614 685 2414
Fragments arranged by size:
2414 685 663 614 217 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4688
PvuII CAG'CTG
Cuts at: 471 1347 2113 2473 471
Size: 876 766 360 2686
Fragments arranged by size:
2686 876 766 360
RsrII CG'GwC_CG
Cuts at: 2883 2883
Size: 4688
SacI G_AGCT'C
Cuts at: 1258 1702 1258
Size: 444 4244
SacII CC_GC'GG
Cuts at: 557 557
Size: 4688
SalI G'TCGA_C
Cuts at: 1725 1725
Size: 4688
SanDI GG'GwC_CC
Cuts at: 421 974 421
Size: 553 4135
SapI GCTCTTCn'nnn_
Cuts at: 273 2711 2921 273
Size: 2438 210 2040
Fragments arranged by size:
2438 2040 210
SbfI CC_TGCA'GG
Cuts at: 1735 1735
Size: 4688
ScaI AGT'ACT
Cuts at: 4205 4205
Size: 4688
SfcI C'TryA_G
Cuts at: 33 142 237 454 840 1117 1731 2416 3409
Size: 109 95 217 386 277 614 685 993
Cuts at: 3409 3600 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 614 386 277 217 191
109 95
SfoI GGC'GCC
Cuts at: 371 2120 2368 371
Size: 1749 248 2691
Fragments arranged by size:
2691 1749 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4688
SgrAI Cr'CCGG_yG
Cuts at: 538 538
Size: 4688
SmaI CCC'GGG
Cuts at: 1710 1710
Size: 4688
SmlI C'TyrA_G
Cuts at: 1843 2758 3250 3512 1843
Size: 915 492 262 3019
Fragments arranged by size:
3019 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4688
SphI G_CATG'C
Cuts at: 1741 2241 1741
Size: 500 4188
StuI AGG'CCT
Cuts at: 431 613 431
Size: 182 4506
StyI C'CwwG_G
Cuts at: 806 1294 1333 1473 1822 2176 806
Size: 488 39 140 349 354 3318
Fragments arranged by size:
3318 488 354 349 140 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 144 2271 2404 2863 3072 144
Size: 2127 133 459 209 1760
Fragments arranged by size:
2127 1760 459 209 133
TatI w'GTAC_w
Cuts at: 1382 4203 1382
Size: 2821 1867
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1152 1184 1364 1396 4205 4237 1152
Size: 32 180 32 2809 32 1603
Fragments arranged by size:
2809 1603 180 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 658 978 2485 658
Size: 320 1507 2861
Fragments arranged by size:
2861 1507 320
XbaI T'CTAG_A
Cuts at: 1719 1719
Size: 4688
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1087 1146 2183 1087
Size: 59 1037 3592
Fragments arranged by size:
3592 1037 59
XmaI C'CCGG_G
Cuts at: 1708 1708
Size: 4688
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BcgI
BfrBI BglI BmgBI BmtI BsgI BsiWI BsmI BsrGI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SexAI SfiI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI