Restriction map for
pF1KB5895
(Circular) (Six-base) MAPSORT of: pF1KB5895.seq Check: 7033 from: 1 to: 4610
pF1KB5895 4610 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1648 1648
Size: 4610
Acc65I G'GTAC_C
Cuts at: 1626 1626
Size: 4610
AclI AA'CG_TT
Cuts at: 4450 4450
Size: 4610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1014 1477 2435 2867 3614 3882 448
Size: 566 463 958 432 747 268 1176
Fragments arranged by size:
1176 958 747 566 463 432 268
AflIII A'CryG_T
Cuts at: 317 621 623 1962 3066 4043 317
Size: 304 2 1339 1104 977 884
Fragments arranged by size:
1339 1104 977 884 304 2
AgeI A'CCGG_T
Cuts at: 2998 2998
Size: 4610
AlwNI CAG_nnn'CTG
Cuts at: 1041 1915 3482 1041
Size: 874 1567 2169
Fragments arranged by size:
2169 1567 874
ApaLI G'TGCA_C
Cuts at: 617 955 3380 617
Size: 338 2425 1847
Fragments arranged by size:
2425 1847 338
ApoI r'AATT_y
Cuts at: 1262 1614 3963 4219 1262
Size: 352 2349 256 1653
Fragments arranged by size:
2349 1653 352 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4610
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
AvaI C'yCGr_G
Cuts at: 779 1630 779
Size: 851 3759
BamHI G'GATC_C
Cuts at: 1635 1635
Size: 4610
BanI G'GyrC_C
Cuts at: 1626 2040 2288 2323 1626
Size: 414 248 35 3913
Fragments arranged by size:
3913 414 248 35
BanII G_rGCy'C
Cuts at: 216 853 884 1143 1624 2654 216
Size: 637 31 259 481 1030 2172
Fragments arranged by size:
2172 1030 637 481 259 31
BbeI G_GCGC'C
Cuts at: 2044 2292 2044
Size: 248 4362
BbsI GAAGACnn'nnnn_
Cuts at: 259 1465 259
Size: 1206 3404
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 519 553 562 596 630 664 519
Size: 34 9 34 34 34 4465
Fragments arranged by size:
4465 34 34 34 34 9
BciVI GTATCCnnnnn_n'
Cuts at: 2508 3269 4610 2508
Size: 761 1341 2508
Fragments arranged by size:
2508 1341 761
BclI T'GATC_A
Cuts at: 1346 1352 2130 1346
Size: 6 778 3826
Fragments arranged by size:
3826 778 6
BfrBI ATG'CAT
Cuts at: 152 152
Size: 4610
BglI GCCn_nnn'nGGC
Cuts at: 527 1445 527
Size: 918 3692
BglII A'GATC_T
Cuts at: 2125 4162 2125
Size: 2037 2573
BlpI GC'TnA_GC
Cuts at: 1722 1722
Size: 4610
Bme1580I G_kGCm'C
Cuts at: 174 621 720 959 2235 2328 3384 174
Size: 447 99 239 1276 93 1056 1400
Fragments arranged by size:
1400 1276 1056 447 239 99 93
BmgBI CAC'GTC
Cuts at: 1089 1089
Size: 4610
BmrI ACTGGGnnnn_n'
Cuts at: 1137 1822 1903 2238 4382 1137
Size: 685 81 335 2144 1365
Fragments arranged by size:
2144 1365 685 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2977 2977
Size: 4610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 722 580
Size: 142 4468
Bpu10I CC'TnA_GC
Cuts at: 796 1048 796
Size: 252 4358
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 1786 2665 3157 3455 200
Size: 1586 879 492 298 1355
Fragments arranged by size:
1586 1355 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4314 4314
Size: 4610
BsaAI yAC'GTr
Cuts at: 624 1963 624
Size: 1339 3271
BsaBI GATnn'nnATC
Cuts at: 6 301 364 1351 2124 4161 6
Size: 295 63 987 773 2037 455
Fragments arranged by size:
2037 987 773 455 295 63
BsaHI Gr'CG_yC
Cuts at: 1204 2041 2289 2991 4487 1204
Size: 837 248 702 1496 1327
Fragments arranged by size:
1496 1327 837 702 248
BsaWI w'CCGG_w
Cuts at: 312 551 1799 2320 2998 3272 3419 3830 312
Size: 239 1248 521 678 274 147 411 1092
Fragments arranged by size:
1248 1092 678 521 411 274 239 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 835 865 835
Size: 30 4580
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 333 333
Size: 4610
BseYI C'CCAG_C
Cuts at: 443 721 899 1077 1487 2035 2793 3370 3866
Size: 278 178 178 410 548 758 577 496
Cuts at: 3866 443
Size: 1187
Fragments arranged by size:
1187 758 577 548 496 410 278 178
178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 1397 964
Size: 433 4177
BsiEI CG_ry'CG
Cuts at: 104 952 2152 2198 3406 104
Size: 848 1200 46 1208 1308
Fragments arranged by size:
1308 1208 1200 848 46
BsiHKAI G_wGCw'C
Cuts at: 216 621 788 884 959 1429 1624 2402 2592
Size: 405 167 96 75 470 195 778 190
Cuts at: 2592 3384 216
Size: 792 1442
Fragments arranged by size:
1442 792 778 470 405 195 190 167
96 75
BsmBI CGTCTCn'nnnn_
Cuts at: 1195 1195
Size: 4610
Bsp1286I G_dGCh'C
Cuts at: 174 216 621 720 788 853 884 959 1143
Size: 42 405 99 68 65 31 75 184
Cuts at: 1143 1429 1624 2235 2328 2402 2592 2654 3384
Size: 286 195 611 93 74 190 62 730
Cuts at: 3384 174
Size: 1400
Fragments arranged by size:
1400 730 611 405 286 195 190 184
99 93 75 74 68 65 62 42
31
BspEI T'CCGG_A
Cuts at: 312 3830 312
Size: 3518 1092
BspHI T'CATG_A
Cuts at: 1349 3786 1349
Size: 2437 2173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1660 1874 2176 2557 1660
Size: 214 302 381 3713
Fragments arranged by size:
3713 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2902 2956 4440 4605 50
Size: 2852 54 1484 165 55
Fragments arranged by size:
2852 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2522 2522
Size: 4610
BsrFI r'CCGG_y
Cuts at: 438 637 985 2608 2789 2998 438
Size: 199 348 1623 181 209 2050
Fragments arranged by size:
2050 1623 348 209 199 181
BssSI C'ACGA_G
Cuts at: 943 2881 3239 943
Size: 1938 358 2314
Fragments arranged by size:
2314 1938 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1987 1987
Size: 4610
BstBI TT'CG_AA
Cuts at: 300 2971 300
Size: 2671 1939
BstXI CCAn_nnnn'nTGG
Cuts at: 141 756 1275 141
Size: 615 519 3476
Fragments arranged by size:
3476 615 519
BstYI r'GATC_y
Cuts at: 1 404 1178 1635 2102 2125 2460 2706 3718
Size: 403 774 457 467 23 335 246 1012
Cuts at: 3718 3804 3816 4162 1
Size: 86 12 346 449
Fragments arranged by size:
1012 774 467 457 449 403 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 369 692 1102 1420 107
Size: 262 323 410 318 3297
Fragments arranged by size:
3297 410 323 318 262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 486 2560 2741 2904 486
Size: 2074 181 163 2192
Fragments arranged by size:
2192 2074 181 163
BtsI GCAGTG_nn'
Cuts at: 1385 1853 1385
Size: 468 4142
DraI TTT'AAA
Cuts at: 1609 1609
Size: 4610
DrdI GACnn_nn'nnGTC
Cuts at: 2316 3174 2316
Size: 858 3752
EaeI y'GGCC_r
Cuts at: 419 2195 2369 2760 2787 4132 419
Size: 1776 174 391 27 1345 897
Fragments arranged by size:
1776 1345 897 391 174 27
EagI C'GGCC_G
Cuts at: 2195 2195
Size: 4610
EarI CTCTTCn'nnn_
Cuts at: 204 551 1129 2633 2843 204
Size: 347 578 1504 210 1971
Fragments arranged by size:
1971 1504 578 347 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1081 2818 3128 3274 4420 1081
Size: 1737 310 146 1146 1271
Fragments arranged by size:
1737 1271 1146 310 146
EcoICRI GAG'CTC
Cuts at: 214 882 1622 214
Size: 668 740 3202
Fragments arranged by size:
3202 740 668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 580 722 1014 1477 2435 2867 3614 3882
Size: 132 142 292 463 958 432 747 268
Cuts at: 3882 448
Size: 1176
Fragments arranged by size:
1176 958 747 463 432 292 268 142
132
EcoO109I rG'GnC_Cy
Cuts at: 697 1038 1749 697
Size: 341 711 3558
Fragments arranged by size:
3558 711 341
EcoRI G'AATT_C
Cuts at: 1614 1614
Size: 4610
EcoRV GAT'ATC
Cuts at: 1335 1335
Size: 4610
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1140 2629 1140
Size: 1489 3121
FspI TGC'GCA
Cuts at: 2391 2391
Size: 4610
HaeII r_GCGC'y
Cuts at: 2044 2292 3314 4293 2044
Size: 248 1022 979 2361
Fragments arranged by size:
2361 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 251 283 2452 2482 2484 2514 251
Size: 32 2169 30 2 30 2347
Fragments arranged by size:
2347 2169 32 30 30 2
HincII GTy'rAC
Cuts at: 1649 1649
Size: 4610
KasI G'GCGC_C
Cuts at: 2040 2288 2040
Size: 248 4362
KpnI G_GTAC'C
Cuts at: 1630 1630
Size: 4610
MluI A'CGCG_T
Cuts at: 4043 4043
Size: 4610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3281 3465 3281
Size: 184 4426
MscI TGG'CCA
Cuts at: 2371 2371
Size: 4610
MslI CAynn'nnrTG
Cuts at: 2599 2726 2599
Size: 127 4483
MspA1I CmG'CkG
Cuts at: 694 1067 1104 1721 2035 2395 3408 3653 694
Size: 373 37 617 314 360 1013 245 1651
Fragments arranged by size:
1651 1013 617 373 360 314 245 37
NaeI GCC'GGC
Cuts at: 440 639 987 2791 440
Size: 199 348 1804 2259
Fragments arranged by size:
2259 1804 348 199
NarI GG'CG_CC
Cuts at: 2041 2289 2041
Size: 248 4362
NcoI C'CATG_G
Cuts at: 107 1420 107
Size: 1313 3297
NgoMIV G'CCGG_C
Cuts at: 438 637 985 2789 438
Size: 199 348 1804 2259
Fragments arranged by size:
2259 1804 348 199
NsiI A_TGCA'T
Cuts at: 154 154
Size: 4610
NspI r_CATG'y
Cuts at: 1663 2163 3070 1663
Size: 500 907 3203
Fragments arranged by size:
3203 907 500
PciI A'CATG_T
Cuts at: 3066 3066
Size: 4610
PflMI CCAn_nnn'nTGG
Cuts at: 1394 1394
Size: 4610
PmeI GTTT'AAAC
Cuts at: 1609 1609
Size: 4610
PmlI CAC'GTG
Cuts at: 624 624
Size: 4610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 4578
PpuMI rG'GwC_Cy
Cuts at: 697 697
Size: 4610
PshAI GACnn'nnGTC
Cuts at: 116 116
Size: 4610
PstI C_TGCA'G
Cuts at: 1657 2342 1657
Size: 685 3925
PvuI CG_AT'CG
Cuts at: 104 952 104
Size: 848 3762
PvuII CAG'CTG
Cuts at: 2035 2395 2035
Size: 360 4250
RsrII CG'GwC_CG
Cuts at: 2805 2805
Size: 4610
SacI G_AGCT'C
Cuts at: 216 884 1624 216
Size: 668 740 3202
Fragments arranged by size:
3202 740 668
SacII CC_GC'GG
Cuts at: 695 1105 695
Size: 410 4200
SalI G'TCGA_C
Cuts at: 1647 1647
Size: 4610
SapI GCTCTTCn'nnn_
Cuts at: 204 551 1129 2633 2843 204
Size: 347 578 1504 210 1971
Fragments arranged by size:
1971 1504 578 347 210
SbfI CC_TGCA'GG
Cuts at: 1657 1657
Size: 4610
ScaI AGT'ACT
Cuts at: 4127 4127
Size: 4610
SfcI C'TryA_G
Cuts at: 33 885 1653 2338 3331 3522 33
Size: 852 768 685 993 191 1121
Fragments arranged by size:
1121 993 852 768 685 191
SfoI GGC'GCC
Cuts at: 2042 2290 2042
Size: 248 4362
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4610
SmaI CCC'GGG
Cuts at: 781 1632 781
Size: 851 3759
SmlI C'TyrA_G
Cuts at: 215 1765 2680 3172 3434 215
Size: 1550 915 492 262 1391
Fragments arranged by size:
1550 1391 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4610
SphI G_CATG'C
Cuts at: 1663 2163 1663
Size: 500 4110
StuI AGG'CCT
Cuts at: 1448 1448
Size: 4610
StyI C'CwwG_G
Cuts at: 107 174 1420 1744 2098 107
Size: 67 1246 324 354 2619
Fragments arranged by size:
2619 1246 354 324 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 604 937 2193 2326 2785 2994 604
Size: 333 1256 133 459 209 2220
Fragments arranged by size:
2220 1256 459 333 209 133
TatI w'GTAC_w
Cuts at: 4125 4125
Size: 4610
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4127 4159 4127
Size: 32 4578
Tth111I GACn'n_nGTC
Cuts at: 1402 2407 1402
Size: 1005 3605
XbaI T'CTAG_A
Cuts at: 1641 1641
Size: 4610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2105 2105
Size: 4610
XmaI C'CCGG_G
Cuts at: 779 1630 779
Size: 851 3759
XmnI GAAnn'nnTTC
Cuts at: 1027 1027
Size: 4610
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BmtI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PfoI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI