Restriction map for
pF1KB5880
(Circular) (Six-base) MAPSORT of: pF1KB5880.seq Check: 7631 from: 1 to: 4601
pF1KB5880 4601 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 994 994
Size: 4601
AccI GT'mk_AC
Cuts at: 1639 1639
Size: 4601
Acc65I G'GTAC_C
Cuts at: 1617 1617
Size: 4601
AclI AA'CG_TT
Cuts at: 1379 4441 1379
Size: 3062 1539
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2426 2858 3605 3873 2426
Size: 432 747 268 3154
Fragments arranged by size:
3154 747 432 268
AflIII A'CryG_T
Cuts at: 665 1953 3057 4034 665
Size: 1288 1104 977 1232
Fragments arranged by size:
1288 1232 1104 977
AgeI A'CCGG_T
Cuts at: 2989 2989
Size: 4601
AleI CACnn'nnGTG
Cuts at: 1261 1261
Size: 4601
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1213 1245 1213
Size: 32 4569
AlwNI CAG_nnn'CTG
Cuts at: 1906 3473 1906
Size: 1567 3034
ApaLI G'TGCA_C
Cuts at: 1153 3371 1153
Size: 2218 2383
ApoI r'AATT_y
Cuts at: 1605 3954 4210 1605
Size: 2349 256 1996
Fragments arranged by size:
2349 1996 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4601
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
AvaI C'yCGr_G
Cuts at: 1621 1621
Size: 4601
BamHI G'GATC_C
Cuts at: 371 1626 371
Size: 1255 3346
BanI G'GyrC_C
Cuts at: 144 982 1617 2031 2279 2314 144
Size: 838 635 414 248 35 2431
Fragments arranged by size:
2431 838 635 414 248 35
BanII G_rGCy'C
Cuts at: 554 708 1615 2645 554
Size: 154 907 1030 2510
Fragments arranged by size:
2510 1030 907 154
BbeI G_GCGC'C
Cuts at: 2035 2283 2035
Size: 248 4353
BciVI GTATCCnnnnn_n'
Cuts at: 462 2499 3260 4601 462
Size: 2037 761 1341 462
Fragments arranged by size:
2037 1341 761 462
BclI T'GATC_A
Cuts at: 2121 2121
Size: 4601
BglII A'GATC_T
Cuts at: 2116 4153 2116
Size: 2037 2564
BlpI GC'TnA_GC
Cuts at: 1713 1713
Size: 4601
Bme1580I G_kGCm'C
Cuts at: 1157 2226 2319 3375 1157
Size: 1069 93 1056 2383
Fragments arranged by size:
2383 1069 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 726 1813 1894 2229 4373 726
Size: 1087 81 335 2144 954
Fragments arranged by size:
2144 1087 954 335 81
BmtI G_CTAG'C
Cuts at: 446 446
Size: 4601
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 420 902 2968 420
Size: 482 2066 2053
Fragments arranged by size:
2066 2053 482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 872 946 1513 284
Size: 588 74 567 3372
Fragments arranged by size:
3372 588 567 74
Bpu10I CC'TnA_GC
Cuts at: 1048 1300 1048
Size: 252 4349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 744 1486 1777 2656 3148 3446 744
Size: 742 291 879 492 298 1899
Fragments arranged by size:
1899 879 742 492 298 291
BsaI GGTCTCn'nnnn_
Cuts at: 762 4305 762
Size: 3543 1058
BsaAI yAC'GTr
Cuts at: 1954 1954
Size: 4601
BsaBI GATnn'nnATC
Cuts at: 6 2115 4152 6
Size: 2109 2037 455
Fragments arranged by size:
2109 2037 455
BsaHI Gr'CG_yC
Cuts at: 1418 2032 2280 2982 4478 1418
Size: 614 248 702 1496 1541
Fragments arranged by size:
1541 1496 702 614 248
BsaWI w'CCGG_w
Cuts at: 1790 2311 2989 3263 3410 3821 1790
Size: 521 678 274 147 411 2570
Fragments arranged by size:
2570 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1213 1243 1213
Size: 30 4571
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 451 1153 1228 229
Size: 222 702 75 3602
Fragments arranged by size:
3602 702 222 75
BseYI C'CCAG_C
Cuts at: 489 795 1114 1558 2026 2784 3361 3857 489
Size: 306 319 444 468 758 577 496 1233
Fragments arranged by size:
1233 758 577 496 468 444 319 306
BsiEI CG_ry'CG
Cuts at: 104 603 2143 2189 3397 104
Size: 499 1540 46 1208 1308
Fragments arranged by size:
1540 1308 1208 499 46
BsiHKAI G_wGCw'C
Cuts at: 194 1157 1226 1615 2393 2583 3375 194
Size: 963 69 389 778 190 792 1420
Fragments arranged by size:
1420 963 792 778 389 190 69
Bsp1286I G_dGCh'C
Cuts at: 194 554 708 1157 1226 1615 2226 2319 2393
Size: 360 154 449 69 389 611 93 74
Cuts at: 2393 2583 2645 3375 194
Size: 190 62 730 1420
Fragments arranged by size:
1420 730 611 449 389 360 190 154
93 74 69 62
BspEI T'CCGG_A
Cuts at: 3821 3821
Size: 4601
BspHI T'CATG_A
Cuts at: 1202 1328 1409 3777 1202
Size: 126 81 2368 2026
Fragments arranged by size:
2368 2026 126 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 429 980 994 1535 1651 1865 2167 2548 429
Size: 551 14 541 116 214 302 381 2482
Fragments arranged by size:
2482 551 541 381 302 214 116 14
BsrBI CCG'CTC
Cuts at: 50 2893 2947 4431 4596 50
Size: 2843 54 1484 165 55
Fragments arranged by size:
2843 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2513 2513
Size: 4601
BsrFI r'CCGG_y
Cuts at: 141 2599 2780 2989 141
Size: 2458 181 209 1753
Fragments arranged by size:
2458 1753 209 181
BsrGI T'GTAC_A
Cuts at: 623 623
Size: 4601
BssSI C'ACGA_G
Cuts at: 2872 3230 2872
Size: 358 4243
BstAPI GCAn_nnn'nTGC
Cuts at: 203 1978 203
Size: 1775 2826
BstBI TT'CG_AA
Cuts at: 2962 2962
Size: 4601
BstXI CCAn_nnnn'nTGG
Cuts at: 432 722 432
Size: 290 4311
BstYI r'GATC_y
Cuts at: 1 371 1467 1626 2093 2116 2451 2697 3709
Size: 370 1096 159 467 23 335 246 1012
Cuts at: 3709 3795 3807 4153 1
Size: 86 12 346 449
Fragments arranged by size:
1096 1012 467 449 370 346 335 246
159 86 23 12
BtgI C'CryG_G
Cuts at: 107 431 510 518 1015 107
Size: 324 79 8 497 3693
Fragments arranged by size:
3693 497 324 79 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2551 2732 2895 2551
Size: 181 163 4257
Fragments arranged by size:
4257 181 163
BtsI GCAGTG_nn'
Cuts at: 137 192 318 950 1844 137
Size: 55 126 632 894 2894
Fragments arranged by size:
2894 894 632 126 55
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 219 254 1004 1039 219
Size: 35 750 35 3781
Fragments arranged by size:
3781 750 35 35
DraI TTT'AAA
Cuts at: 1600 1600
Size: 4601
DrdI GACnn_nn'nnGTC
Cuts at: 1423 2307 3165 1423
Size: 884 858 2859
Fragments arranged by size:
2859 884 858
EaeI y'GGCC_r
Cuts at: 183 712 2186 2360 2751 2778 4123 183
Size: 529 1474 174 391 27 1345 661
Fragments arranged by size:
1474 1345 661 529 391 174 27
EagI C'GGCC_G
Cuts at: 2186 2186
Size: 4601
EarI CTCTTCn'nnn_
Cuts at: 1403 2624 2834 1403
Size: 1221 210 3170
Fragments arranged by size:
3170 1221 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 532 2809 3119 3265 4411 127
Size: 405 2277 310 146 1146 317
Fragments arranged by size:
2277 1146 405 317 310 146
EcoICRI GAG'CTC
Cuts at: 1613 1613
Size: 4601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 872 946 1513 2426 2858 3605 3873 284
Size: 588 74 567 913 432 747 268 1012
Fragments arranged by size:
1012 913 747 588 567 432 268 74
EcoNI CCTnn'n_nnAGG
Cuts at: 565 565
Size: 4601
EcoO109I rG'GnC_Cy
Cuts at: 418 1740 418
Size: 1322 3279
EcoRI G'AATT_C
Cuts at: 1605 1605
Size: 4601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 4601
FspI TGC'GCA
Cuts at: 2382 2382
Size: 4601
HaeII r_GCGC'y
Cuts at: 791 2035 2283 3305 4284 791
Size: 1244 248 1022 979 1108
Fragments arranged by size:
1244 1108 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2443 2473 2475 2505 2443
Size: 30 2 30 4539
Fragments arranged by size:
4539 30 30 2
HincII GTy'rAC
Cuts at: 1640 1640
Size: 4601
KasI G'GCGC_C
Cuts at: 2031 2279 2031
Size: 248 4353
KpnI G_GTAC'C
Cuts at: 1621 1621
Size: 4601
MfeI C'AATT_G
Cuts at: 1545 1545
Size: 4601
MluI A'CGCG_T
Cuts at: 4034 4034
Size: 4601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 745 1363 1560 3272 3456 745
Size: 618 197 1712 184 1890
Fragments arranged by size:
1890 1712 618 197 184
MscI TGG'CCA
Cuts at: 2362 2362
Size: 4601
MslI CAynn'nnrTG
Cuts at: 812 1261 1327 2590 2717 812
Size: 449 66 1263 127 2696
Fragments arranged by size:
2696 1263 449 127 66
MspA1I CmG'CkG
Cuts at: 153 1114 1712 2026 2386 3399 3644 153
Size: 961 598 314 360 1013 245 1110
Fragments arranged by size:
1110 1013 961 598 360 314 245
NaeI GCC'GGC
Cuts at: 143 2782 143
Size: 2639 1962
NarI GG'CG_CC
Cuts at: 2032 2280 2032
Size: 248 4353
NcoI C'CATG_G
Cuts at: 107 431 510 107
Size: 324 79 4198
Fragments arranged by size:
4198 324 79
NgoMIV G'CCGG_C
Cuts at: 141 2780 141
Size: 2639 1962
NheI G'CTAG_C
Cuts at: 442 442
Size: 4601
NspI r_CATG'y
Cuts at: 669 1654 2154 3061 669
Size: 985 500 907 2209
Fragments arranged by size:
2209 985 907 500
PasI CC'CwG_GG
Cuts at: 567 567
Size: 4601
PciI A'CATG_T
Cuts at: 665 3057 665
Size: 2392 2209
PfoI T'CCnGG_A
Cuts at: 374 1490 374
Size: 1116 3485
PmeI GTTT'AAAC
Cuts at: 1600 1600
Size: 4601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1213 1245 3740 3772 1213
Size: 32 2495 32 2042
Fragments arranged by size:
2495 2042 32 32
PpuMI rG'GwC_Cy
Cuts at: 418 418
Size: 4601
PstI C_TGCA'G
Cuts at: 199 991 1532 1648 2333 199
Size: 792 541 116 685 2467
Fragments arranged by size:
2467 792 685 541 116
PvuI CG_AT'CG
Cuts at: 104 603 104
Size: 499 4102
PvuII CAG'CTG
Cuts at: 1114 2026 2386 1114
Size: 912 360 3329
Fragments arranged by size:
3329 912 360
RsrII CG'GwC_CG
Cuts at: 2796 2796
Size: 4601
SacI G_AGCT'C
Cuts at: 1615 1615
Size: 4601
SalI G'TCGA_C
Cuts at: 1638 1638
Size: 4601
SapI GCTCTTCn'nnn_
Cuts at: 2624 2834 2624
Size: 210 4391
SbfI CC_TGCA'GG
Cuts at: 991 1648 991
Size: 657 3944
ScaI AGT'ACT
Cuts at: 4118 4118
Size: 4601
SexAI A'CCwGG_T
Cuts at: 1168 1168
Size: 4601
SfcI C'TryA_G
Cuts at: 33 195 987 1528 1644 2329 3322 3513 33
Size: 162 792 541 116 685 993 191 1121
Fragments arranged by size:
1121 993 792 685 541 191 162 116
SfoI GGC'GCC
Cuts at: 2033 2281 2033
Size: 248 4353
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
SmaI CCC'GGG
Cuts at: 1623 1623
Size: 4601
SmlI C'TyrA_G
Cuts at: 759 1501 1756 2671 3163 3425 759
Size: 742 255 915 492 262 1935
Fragments arranged by size:
1935 915 742 492 262 255
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4601
SphI G_CATG'C
Cuts at: 1654 2154 1654
Size: 500 4101
StyI C'CwwG_G
Cuts at: 107 431 510 1735 2089 107
Size: 324 79 1225 354 2619
Fragments arranged by size:
2619 1225 354 324 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 523 992 1402 2184 2317 2776 2985 523
Size: 469 410 782 133 459 209 2139
Fragments arranged by size:
2139 782 469 459 410 209 133
TatI w'GTAC_w
Cuts at: 623 4116 623
Size: 3493 1108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4118 4150 4118
Size: 32 4569
Tth111I GACn'n_nGTC
Cuts at: 2398 2398
Size: 4601
XbaI T'CTAG_A
Cuts at: 1401 1632 1401
Size: 231 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 739 2096 739
Size: 1357 3244
XmaI C'CCGG_G
Cuts at: 1621 1621
Size: 4601
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI NdeI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI