Restriction map for
pF1KB5876
(Circular) (Six-base) MAPSORT of: pF1KB5876.seq Check: 7672 from: 1 to: 4598
pF1KB5876 4598 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1636 1636
Size: 4598
Acc65I G'GTAC_C
Cuts at: 1614 1614
Size: 4598
AclI AA'CG_TT
Cuts at: 1435 4438 1435
Size: 3003 1595
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 1283 2423 2855 3602 3870 552
Size: 731 1140 432 747 268 1280
Fragments arranged by size:
1280 1140 747 731 432 268
AflIII A'CryG_T
Cuts at: 683 1950 3054 4031 683
Size: 1267 1104 977 1250
Fragments arranged by size:
1267 1250 1104 977
AgeI A'CCGG_T
Cuts at: 2986 2986
Size: 4598
AhdI GACnn_n'nnGTC
Cuts at: 393 393
Size: 4598
AleI CACnn'nnGTG
Cuts at: 1055 1055
Size: 4598
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1443 1475 1443
Size: 32 4566
AlwNI CAG_nnn'CTG
Cuts at: 1036 1903 3470 1036
Size: 867 1567 2164
Fragments arranged by size:
2164 1567 867
ApaI G_GGCC'C
Cuts at: 185 185
Size: 4598
ApaLI G'TGCA_C
Cuts at: 3368 3368
Size: 4598
ApoI r'AATT_y
Cuts at: 438 525 556 1347 1602 3951 4207 438
Size: 87 31 791 255 2349 256 829
Fragments arranged by size:
2349 829 791 256 255 87 31
AseI AT'TA_AT
Cuts at: 20 20
Size: 4598
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
AvaI C'yCGr_G
Cuts at: 160 331 367 1618 160
Size: 171 36 1251 3140
Fragments arranged by size:
3140 1251 171 36
BamHI G'GATC_C
Cuts at: 1623 1623
Size: 4598
BanI G'GyrC_C
Cuts at: 1614 2028 2276 2311 1614
Size: 414 248 35 3901
Fragments arranged by size:
3901 414 248 35
BanII G_rGCy'C
Cuts at: 185 339 1612 2642 185
Size: 154 1273 1030 2141
Fragments arranged by size:
2141 1273 1030 154
BbeI G_GCGC'C
Cuts at: 2032 2280 2032
Size: 248 4350
BbsI GAAGACnn'nnnn_
Cuts at: 467 467
Size: 4598
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1510 1544 1510
Size: 34 4564
BciVI GTATCCnnnnn_n'
Cuts at: 1557 2496 3257 4598 1557
Size: 939 761 1341 1557
Fragments arranged by size:
1557 1341 939 761
BclI T'GATC_A
Cuts at: 2118 2118
Size: 4598
BfrBI ATG'CAT
Cuts at: 768 768
Size: 4598
BglII A'GATC_T
Cuts at: 2113 4150 2113
Size: 2037 2561
BlpI GC'TnA_GC
Cuts at: 113 1710 113
Size: 1597 3001
Bme1580I G_kGCm'C
Cuts at: 185 2223 2316 3372 185
Size: 2038 93 1056 1411
Fragments arranged by size:
2038 1411 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 315 387 575 1810 1891 2226 4370 315
Size: 72 188 1235 81 335 2144 543
Fragments arranged by size:
2144 1235 543 335 188 81 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2965 2965
Size: 4598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 788 788
Size: 4598
Bpu10I CC'TnA_GC
Cuts at: 187 187
Size: 4598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1774 2653 3145 3443 1774
Size: 879 492 298 2929
Fragments arranged by size:
2929 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4302 4302
Size: 4598
BsaAI yAC'GTr
Cuts at: 1951 1951
Size: 4598
BsaBI GATnn'nnATC
Cuts at: 6 2112 4149 6
Size: 2106 2037 455
Fragments arranged by size:
2106 2037 455
BsaHI Gr'CG_yC
Cuts at: 353 389 2029 2277 2979 4475 353
Size: 36 1640 248 702 1496 476
Fragments arranged by size:
1640 1496 702 476 248 36
BsaWI w'CCGG_w
Cuts at: 1080 1488 1787 2308 2986 3260 3407 3818 1080
Size: 408 299 521 678 274 147 411 1860
Fragments arranged by size:
1860 678 521 411 408 299 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 307 337 307
Size: 30 4568
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 920 1156 920
Size: 236 4362
BseYI C'CCAG_C
Cuts at: 421 2023 2781 3358 3854 421
Size: 1602 758 577 496 1165
Fragments arranged by size:
1602 1165 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 713 713
Size: 4598
BsiEI CG_ry'CG
Cuts at: 104 2140 2186 3394 104
Size: 2036 46 1208 1308
Fragments arranged by size:
2036 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1308 1612 2390 2580 3372 1308
Size: 304 778 190 792 2534
Fragments arranged by size:
2534 792 778 304 190
BsmBI CGTCTCn'nnnn_
Cuts at: 161 161
Size: 4598
Bsp1286I G_dGCh'C
Cuts at: 185 339 1308 1612 2223 2316 2390 2580 2642
Size: 154 969 304 611 93 74 190 62
Cuts at: 2642 3372 185
Size: 730 1411
Fragments arranged by size:
1411 969 730 611 304 190 154 93
74 62
BspEI T'CCGG_A
Cuts at: 1488 3818 1488
Size: 2330 2268
BspHI T'CATG_A
Cuts at: 3774 3774
Size: 4598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1648 1862 2164 2545 1648
Size: 214 302 381 3701
Fragments arranged by size:
3701 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2890 2944 4428 4593 50
Size: 2840 54 1484 165 55
Fragments arranged by size:
2840 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 236 2510 236
Size: 2274 2324
BsrFI r'CCGG_y
Cuts at: 2596 2777 2986 2596
Size: 181 209 4208
Fragments arranged by size:
4208 209 181
BssSI C'ACGA_G
Cuts at: 733 2869 3227 733
Size: 2136 358 2104
Fragments arranged by size:
2136 2104 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1975 1975
Size: 4598
BstBI TT'CG_AA
Cuts at: 1442 2959 1442
Size: 1517 3081
BstYI r'GATC_y
Cuts at: 1 1095 1623 2090 2113 2448 2694 3706 3792
Size: 1094 528 467 23 335 246 1012 86
Cuts at: 3792 3804 4150 1
Size: 12 346 449
Fragments arranged by size:
1094 1012 528 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 205 311 107
Size: 98 106 4394
Fragments arranged by size:
4394 106 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2548 2729 2892 2548
Size: 181 163 4254
Fragments arranged by size:
4254 181 163
BtsI GCAGTG_nn'
Cuts at: 194 1308 1528 1841 194
Size: 1114 220 313 2951
Fragments arranged by size:
2951 1114 313 220
DraI TTT'AAA
Cuts at: 657 1597 657
Size: 940 3658
DraIII CAC_nnn'GTG
Cuts at: 458 458
Size: 4598
DrdI GACnn_nn'nnGTC
Cuts at: 2304 3162 2304
Size: 858 3740
EaeI y'GGCC_r
Cuts at: 426 857 2183 2357 2748 2775 4120 426
Size: 431 1326 174 391 27 1345 904
Fragments arranged by size:
1345 1326 904 431 391 174 27
EagI C'GGCC_G
Cuts at: 2183 2183
Size: 4598
EarI CTCTTCn'nnn_
Cuts at: 895 2621 2831 895
Size: 1726 210 2662
Fragments arranged by size:
2662 1726 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 329 2806 3116 3262 4408 329
Size: 2477 310 146 1146 519
Fragments arranged by size:
2477 1146 519 310 146
EcoICRI GAG'CTC
Cuts at: 1610 1610
Size: 4598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 788 1283 2423 2855 3602 3870 552
Size: 236 495 1140 432 747 268 1280
Fragments arranged by size:
1280 1140 747 495 432 268 236
EcoO109I rG'GnC_Cy
Cuts at: 181 182 1737 181
Size: 1 1555 3042
Fragments arranged by size:
3042 1555 1
EcoRI G'AATT_C
Cuts at: 1602 1602
Size: 4598
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1401 2617 1401
Size: 1216 3382
FspI TGC'GCA
Cuts at: 2379 2379
Size: 4598
HaeII r_GCGC'y
Cuts at: 2032 2280 3302 4281 2032
Size: 248 1022 979 2349
Fragments arranged by size:
2349 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 307 339 379 411 966 998 1114 1146 1305
Size: 32 40 32 555 32 116 32 159
Cuts at: 1305 1337 2440 2470 2472 2502 307
Size: 32 1103 30 2 30 2403
Fragments arranged by size:
2403 1103 555 159 116 40 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1637 1637
Size: 4598
HindIII A'AGCT_T
Cuts at: 1383 1383
Size: 4598
KasI G'GCGC_C
Cuts at: 2028 2276 2028
Size: 248 4350
KpnI G_GTAC'C
Cuts at: 1618 1618
Size: 4598
MluI A'CGCG_T
Cuts at: 4031 4031
Size: 4598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 874 3269 3453 874
Size: 2395 184 2019
Fragments arranged by size:
2395 2019 184
MscI TGG'CCA
Cuts at: 859 2359 859
Size: 1500 3098
MslI CAynn'nnrTG
Cuts at: 1055 2587 2714 1055
Size: 1532 127 2939
Fragments arranged by size:
2939 1532 127
MspA1I CmG'CkG
Cuts at: 207 425 1709 2023 2383 3396 3641 207
Size: 218 1284 314 360 1013 245 1164
Fragments arranged by size:
1284 1164 1013 360 314 245 218
NaeI GCC'GGC
Cuts at: 2779 2779
Size: 4598
NarI GG'CG_CC
Cuts at: 2029 2277 2029
Size: 248 4350
NcoI C'CATG_G
Cuts at: 107 311 107
Size: 204 4394
NgoMIV G'CCGG_C
Cuts at: 2777 2777
Size: 4598
NsiI A_TGCA'T
Cuts at: 770 770
Size: 4598
NspI r_CATG'y
Cuts at: 154 687 772 1651 2151 3058 154
Size: 533 85 879 500 907 1694
Fragments arranged by size:
1694 907 879 533 500 85
PciI A'CATG_T
Cuts at: 683 3054 683
Size: 2371 2227
PflMI CCAn_nnn'nTGG
Cuts at: 458 458
Size: 4598
PmeI GTTT'AAAC
Cuts at: 1597 1597
Size: 4598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 3737
Size: 32 4566
PspOMI G'GGCC_C
Cuts at: 181 181
Size: 4598
PstI C_TGCA'G
Cuts at: 519 1645 2330 519
Size: 1126 685 2787
Fragments arranged by size:
2787 1126 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4598
PvuII CAG'CTG
Cuts at: 2023 2383 2023
Size: 360 4238
RsrII CG'GwC_CG
Cuts at: 2793 2793
Size: 4598
SacI G_AGCT'C
Cuts at: 1612 1612
Size: 4598
SacII CC_GC'GG
Cuts at: 208 208
Size: 4598
SalI G'TCGA_C
Cuts at: 1635 1635
Size: 4598
SapI GCTCTTCn'nnn_
Cuts at: 2621 2831 2621
Size: 210 4388
SbfI CC_TGCA'GG
Cuts at: 1645 1645
Size: 4598
ScaI AGT'ACT
Cuts at: 4115 4115
Size: 4598
SexAI A'CCwGG_T
Cuts at: 1286 1286
Size: 4598
SfcI C'TryA_G
Cuts at: 33 515 1641 2326 3319 3510 33
Size: 482 1126 685 993 191 1121
Fragments arranged by size:
1126 1121 993 685 482 191
SfoI GGC'GCC
Cuts at: 2030 2278 2030
Size: 248 4350
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
SmaI CCC'GGG
Cuts at: 333 369 1620 333
Size: 36 1251 3311
Fragments arranged by size:
3311 1251 36
SmlI C'TyrA_G
Cuts at: 1753 2668 3160 3422 1753
Size: 915 492 262 2929
Fragments arranged by size:
2929 915 492 262
SpeI A'CTAG_T
Cuts at: 67 121 67
Size: 54 4544
SphI G_CATG'C
Cuts at: 154 772 1651 2151 154
Size: 618 879 500 2601
Fragments arranged by size:
2601 879 618 500
SspI AAT'ATT
Cuts at: 864 864
Size: 4598
StyI C'CwwG_G
Cuts at: 107 311 433 1169 1732 2086 107
Size: 204 122 736 563 354 2619
Fragments arranged by size:
2619 736 563 354 204 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2181 2314 2773 2982 2181
Size: 133 459 209 3797
Fragments arranged by size:
3797 459 209 133
TatI w'GTAC_w
Cuts at: 4113 4113
Size: 4598
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4115 4147 4115
Size: 32 4566
Tth111I GACn'n_nGTC
Cuts at: 2395 2395
Size: 4598
XbaI T'CTAG_A
Cuts at: 1018 1629 1018
Size: 611 3987
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2093 2093
Size: 4598
XmaI C'CCGG_G
Cuts at: 331 367 1618 331
Size: 36 1251 3311
Fragments arranged by size:
3311 1251 36
XmnI GAAnn'nnTTC
Cuts at: 505 1350 1478 505
Size: 845 128 3625
Fragments arranged by size:
3625 845 128
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AscI AvrII BaeI
BbvCI BglI BmgBI BmtI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspXI
PsrI SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI