Restriction map for
pF1KB5868
(Circular) (Six-base) MAPSORT of: pF1KB5868.seq Check: 297 from: 1 to: 4592
pF1KB5868 4592 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 746 1289 1630 746
Size: 543 341 3708
Fragments arranged by size:
3708 543 341
Acc65I G'GTAC_C
Cuts at: 1608 1608
Size: 4592
AclI AA'CG_TT
Cuts at: 4432 4432
Size: 4592
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2417 2849 3596 3864 2417
Size: 432 747 268 3145
Fragments arranged by size:
3145 747 432 268
AflIII A'CryG_T
Cuts at: 614 1099 1944 3048 4025 614
Size: 485 845 1104 977 1181
Fragments arranged by size:
1181 1104 977 845 485
AgeI A'CCGG_T
Cuts at: 2980 2980
Size: 4592
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 723 723
Size: 4592
AlwNI CAG_nnn'CTG
Cuts at: 490 1897 3464 490
Size: 1407 1567 1618
Fragments arranged by size:
1618 1567 1407
ApaLI G'TGCA_C
Cuts at: 636 3362 636
Size: 2726 1866
ApoI r'AATT_y
Cuts at: 1051 1596 3945 4201 1051
Size: 545 2349 256 1442
Fragments arranged by size:
2349 1442 545 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4592
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4592
AvaI C'yCGr_G
Cuts at: 448 1612 448
Size: 1164 3428
BamHI G'GATC_C
Cuts at: 213 691 1617 213
Size: 478 926 3188
Fragments arranged by size:
3188 926 478
BanI G'GyrC_C
Cuts at: 1608 2022 2270 2305 1608
Size: 414 248 35 3895
Fragments arranged by size:
3895 414 248 35
BanII G_rGCy'C
Cuts at: 1606 2636 1606
Size: 1030 3562
BbeI G_GCGC'C
Cuts at: 2026 2274 2026
Size: 248 4344
BbsI GAAGACnn'nnnn_
Cuts at: 992 992
Size: 4592
BbvCI CC'TCA_GC
Cuts at: 359 545 359
Size: 186 4406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 622 656 1223 1257 622
Size: 34 567 34 3957
Fragments arranged by size:
3957 567 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 301 586 1069 2490 3251 4592 301
Size: 285 483 1421 761 1341 301
Fragments arranged by size:
1421 1341 761 483 301 285
BclI T'GATC_A
Cuts at: 2112 2112
Size: 4592
BglII A'GATC_T
Cuts at: 2107 4144 2107
Size: 2037 2555
BlpI GC'TnA_GC
Cuts at: 902 961 1704 902
Size: 59 743 3790
Fragments arranged by size:
3790 743 59
Bme1580I G_kGCm'C
Cuts at: 640 712 1069 2217 2310 3366 640
Size: 72 357 1148 93 1056 1866
Fragments arranged by size:
1866 1148 1056 357 93 72
BmrI ACTGGGnnnn_n'
Cuts at: 1240 1272 1804 1885 2220 4364 1240
Size: 32 532 81 335 2144 1468
Fragments arranged by size:
2144 1468 532 335 81 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 328 2959 328
Size: 2631 1961
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1522 1522
Size: 4592
Bpu10I CC'TnA_GC
Cuts at: 359 545 359
Size: 186 4406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 993 1768 2647 3139 3437 993
Size: 775 879 492 298 2148
Fragments arranged by size:
2148 879 775 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1117 4296 1117
Size: 3179 1413
BsaAI yAC'GTr
Cuts at: 182 1945 182
Size: 1763 2829
BsaBI GATnn'nnATC
Cuts at: 6 2106 4143 6
Size: 2100 2037 455
Fragments arranged by size:
2100 2037 455
BsaHI Gr'CG_yC
Cuts at: 2023 2271 2973 4469 2023
Size: 248 702 1496 2146
Fragments arranged by size:
2146 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1781 2302 2980 3254 3401 3812 1781
Size: 521 678 274 147 411 2561
Fragments arranged by size:
2561 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 753 783 753
Size: 30 4562
BseYI C'CCAG_C
Cuts at: 534 2017 2775 3352 3848 534
Size: 1483 758 577 496 1278
Fragments arranged by size:
1483 1278 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 843 605
Size: 238 4354
BsiEI CG_ry'CG
Cuts at: 104 2134 2180 3388 104
Size: 2030 46 1208 1308
Fragments arranged by size:
2030 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 640 903 1460 1606 2384 2574 3366 640
Size: 263 557 146 778 190 792 1866
Fragments arranged by size:
1866 792 778 557 263 190 146
BsmI GAATG_Cn'
Cuts at: 551 1003 1141 551
Size: 452 138 4002
Fragments arranged by size:
4002 452 138
Bsp1286I G_dGCh'C
Cuts at: 640 712 903 1069 1460 1606 2217 2310 2384
Size: 72 191 166 391 146 611 93 74
Cuts at: 2384 2574 2636 3366 640
Size: 190 62 730 1866
Fragments arranged by size:
1866 730 611 391 191 190 166 146
93 74 72 62
BspEI T'CCGG_A
Cuts at: 3812 3812
Size: 4592
BspHI T'CATG_A
Cuts at: 3768 3768
Size: 4592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 868 1642 1856 2158 2539 868
Size: 774 214 302 381 2921
Fragments arranged by size:
2921 774 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2884 2938 4422 4587 50
Size: 2834 54 1484 165 55
Fragments arranged by size:
2834 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 196 235 1454 2504 196
Size: 39 1219 1050 2284
Fragments arranged by size:
2284 1219 1050 39
BsrFI r'CCGG_y
Cuts at: 2590 2771 2980 2590
Size: 181 209 4202
Fragments arranged by size:
4202 209 181
BssSI C'ACGA_G
Cuts at: 1459 2863 3221 1459
Size: 1404 358 2830
Fragments arranged by size:
2830 1404 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1969 1969
Size: 4592
BstBI TT'CG_AA
Cuts at: 2953 2953
Size: 4592
BstXI CCAn_nnnn'nTGG
Cuts at: 426 426
Size: 4592
BstYI r'GATC_y
Cuts at: 1 213 691 1206 1617 2084 2107 2442 2688
Size: 212 478 515 411 467 23 335 246
Cuts at: 2688 3700 3786 3798 4144 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 515 478 467 449 411 346 335
246 212 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 390 390
Size: 4592
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2542 2723 2886 2542
Size: 181 163 4248
Fragments arranged by size:
4248 181 163
BtsI GCAGTG_nn'
Cuts at: 638 745 913 972 1835 638
Size: 107 168 59 863 3395
Fragments arranged by size:
3395 863 168 107 59
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 390 425 1550 1585 390
Size: 35 1125 35 3397
Fragments arranged by size:
3397 1125 35 35
DraI TTT'AAA
Cuts at: 1591 1591
Size: 4592
DraIII CAC_nnn'GTG
Cuts at: 615 615
Size: 4592
DrdI GACnn_nn'nnGTC
Cuts at: 1433 2298 3156 1433
Size: 865 858 2869
Fragments arranged by size:
2869 865 858
EaeI y'GGCC_r
Cuts at: 464 504 2177 2351 2742 2769 4114 464
Size: 40 1673 174 391 27 1345 942
Fragments arranged by size:
1673 1345 942 391 174 40 27
EagI C'GGCC_G
Cuts at: 2177 2177
Size: 4592
EarI CTCTTCn'nnn_
Cuts at: 2615 2825 2615
Size: 210 4382
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 543 2800 3110 3256 4402 543
Size: 2257 310 146 1146 733
Fragments arranged by size:
2257 1146 733 310 146
EcoICRI GAG'CTC
Cuts at: 1604 1604
Size: 4592
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1522 2417 2849 3596 3864 1522
Size: 895 432 747 268 2250
Fragments arranged by size:
2250 895 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 487 1529 1731 487
Size: 1042 202 3348
Fragments arranged by size:
3348 1042 202
EcoRI G'AATT_C
Cuts at: 1596 1596
Size: 4592
EcoRV GAT'ATC
Cuts at: 721 721
Size: 4592
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4592
FspI TGC'GCA
Cuts at: 2373 2373
Size: 4592
HaeII r_GCGC'y
Cuts at: 148 2026 2274 3296 4275 148
Size: 1878 248 1022 979 465
Fragments arranged by size:
1878 1022 979 465 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1475 1507 2434 2464 2466 2496 1475
Size: 32 927 30 2 30 3571
Fragments arranged by size:
3571 927 32 30 30 2
HincII GTy'rAC
Cuts at: 294 1631 294
Size: 1337 3255
KasI G'GCGC_C
Cuts at: 2022 2270 2022
Size: 248 4344
KpnI G_GTAC'C
Cuts at: 1612 1612
Size: 4592
MfeI C'AATT_G
Cuts at: 838 838
Size: 4592
MluI A'CGCG_T
Cuts at: 4025 4025
Size: 4592
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 584 1138 3263 3447 584
Size: 554 2125 184 1729
Fragments arranged by size:
2125 1729 554 184
MscI TGG'CCA
Cuts at: 466 2353 466
Size: 1887 2705
MslI CAynn'nnrTG
Cuts at: 705 1062 2581 2708 705
Size: 357 1519 127 2589
Fragments arranged by size:
2589 1519 357 127
MspA1I CmG'CkG
Cuts at: 538 1703 2017 2377 3390 3635 538
Size: 1165 314 360 1013 245 1495
Fragments arranged by size:
1495 1165 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2773 2773
Size: 4592
NarI GG'CG_CC
Cuts at: 2023 2271 2023
Size: 248 4344
NgoMIV G'CCGG_C
Cuts at: 2771 2771
Size: 4592
NspI r_CATG'y
Cuts at: 704 1103 1645 2145 3052 704
Size: 399 542 500 907 2244
Fragments arranged by size:
2244 907 542 500 399
PasI CC'CwG_GG
Cuts at: 1071 1071
Size: 4592
PciI A'CATG_T
Cuts at: 1099 3048 1099
Size: 1949 2643
PfoI T'CCnGG_A
Cuts at: 714 714
Size: 4592
PmeI GTTT'AAAC
Cuts at: 1591 1591
Size: 4592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3763 3731
Size: 32 4560
PpuMI rG'GwC_Cy
Cuts at: 1529 1529
Size: 4592
PstI C_TGCA'G
Cuts at: 480 645 1639 2324 480
Size: 165 994 685 2748
Fragments arranged by size:
2748 994 685 165
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4592
PvuII CAG'CTG
Cuts at: 538 2017 2377 538
Size: 1479 360 2753
Fragments arranged by size:
2753 1479 360
RsrII CG'GwC_CG
Cuts at: 2787 2787
Size: 4592
SacI G_AGCT'C
Cuts at: 1606 1606
Size: 4592
SalI G'TCGA_C
Cuts at: 1629 1629
Size: 4592
SapI GCTCTTCn'nnn_
Cuts at: 2615 2825 2615
Size: 210 4382
SbfI CC_TGCA'GG
Cuts at: 1639 1639
Size: 4592
ScaI AGT'ACT
Cuts at: 4109 4109
Size: 4592
SfcI C'TryA_G
Cuts at: 33 476 641 657 1635 2320 3313 3504 33
Size: 443 165 16 978 685 993 191 1121
Fragments arranged by size:
1121 993 978 685 443 191 165 16
SfoI GGC'GCC
Cuts at: 2024 2272 2024
Size: 248 4344
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4592
SmaI CCC'GGG
Cuts at: 450 1614 450
Size: 1164 3428
SmlI C'TyrA_G
Cuts at: 1008 1747 2662 3154 3416 1008
Size: 739 915 492 262 2184
Fragments arranged by size:
2184 915 739 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4592
SphI G_CATG'C
Cuts at: 1645 2145 1645
Size: 500 4092
StyI C'CwwG_G
Cuts at: 1726 2080 1726
Size: 354 4238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2175 2308 2767 2976 2175
Size: 133 459 209 3791
Fragments arranged by size:
3791 459 209 133
TatI w'GTAC_w
Cuts at: 4107 4107
Size: 4592
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1067 1099 1138 1170 4109 4141 1067
Size: 32 39 32 2939 32 1518
Fragments arranged by size:
2939 1518 39 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 256 2389 256
Size: 2133 2459
XbaI T'CTAG_A
Cuts at: 1623 1623
Size: 4592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 826 2087 826
Size: 1261 3331
XmaI C'CCGG_G
Cuts at: 448 1612 448
Size: 1164 3428
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BfrBI BglI BmgBI BmtI BseRI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I BtgI ClaI
EcoNI FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI