Restriction map for
pF1KB5864
(Circular) (Six-base) MAPSORT of: pF1KB5864.seq Check: 8373 from: 1 to: 4586
pF1KB5864 4586 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 224 224
Size: 4586
AccI GT'mk_AC
Cuts at: 1624 1624
Size: 4586
Acc65I G'GTAC_C
Cuts at: 1602 1602
Size: 4586
AclI AA'CG_TT
Cuts at: 4426 4426
Size: 4586
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 1336 2411 2843 3590 3858 857
Size: 479 1075 432 747 268 1585
Fragments arranged by size:
1585 1075 747 479 432 268
AflIII A'CryG_T
Cuts at: 1938 3042 4019 1938
Size: 1104 977 2505
Fragments arranged by size:
2505 1104 977
AgeI A'CCGG_T
Cuts at: 2974 2974
Size: 4586
AleI CACnn'nnGTG
Cuts at: 1517 1517
Size: 4586
AlwNI CAG_nnn'CTG
Cuts at: 144 153 1891 3458 144
Size: 9 1738 1567 1272
Fragments arranged by size:
1738 1567 1272 9
ApaI G_GGCC'C
Cuts at: 180 180
Size: 4586
ApaLI G'TGCA_C
Cuts at: 3356 3356
Size: 4586
ApoI r'AATT_y
Cuts at: 1239 1590 3939 4195 1239
Size: 351 2349 256 1630
Fragments arranged by size:
2349 1630 351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4586
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
AvaI C'yCGr_G
Cuts at: 112 1393 1606 112
Size: 1281 213 3092
Fragments arranged by size:
3092 1281 213
BamHI G'GATC_C
Cuts at: 536 1611 536
Size: 1075 3511
BanI G'GyrC_C
Cuts at: 1602 2016 2264 2299 1602
Size: 414 248 35 3889
Fragments arranged by size:
3889 414 248 35
BanII G_rGCy'C
Cuts at: 164 180 401 1600 2630 164
Size: 16 221 1199 1030 2120
Fragments arranged by size:
2120 1199 1030 221 16
BbeI G_GCGC'C
Cuts at: 2020 2268 2020
Size: 248 4338
BbsI GAAGACnn'nnnn_
Cuts at: 627 627
Size: 4586
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 148 182 90
Size: 34 24 34 4494
Fragments arranged by size:
4494 34 34 24
BciVI GTATCCnnnnn_n'
Cuts at: 2484 3245 4586 2484
Size: 761 1341 2484
Fragments arranged by size:
2484 1341 761
BclI T'GATC_A
Cuts at: 2106 2106
Size: 4586
BglI GCCn_nnn'nGGC
Cuts at: 175 175
Size: 4586
BglII A'GATC_T
Cuts at: 890 2101 4138 890
Size: 1211 2037 1338
Fragments arranged by size:
2037 1338 1211
BlpI GC'TnA_GC
Cuts at: 1698 1698
Size: 4586
Bme1580I G_kGCm'C
Cuts at: 171 180 555 2211 2304 3360 171
Size: 9 375 1656 93 1056 1397
Fragments arranged by size:
1656 1397 1056 375 93 9
BmrI ACTGGGnnnn_n'
Cuts at: 1798 1879 2214 4358 1798
Size: 81 335 2144 2026
Fragments arranged by size:
2144 2026 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 609 2953 609
Size: 2344 2242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 1536 202
Size: 1334 3252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1762 2641 3133 3431 1762
Size: 879 492 298 2917
Fragments arranged by size:
2917 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 301 415 1502 4290 301
Size: 114 1087 2788 597
Fragments arranged by size:
2788 1087 597 114
BsaAI yAC'GTr
Cuts at: 1939 1939
Size: 4586
BsaBI GATnn'nnATC
Cuts at: 6 2100 4137 6
Size: 2094 2037 455
Fragments arranged by size:
2094 2037 455
BsaHI Gr'CG_yC
Cuts at: 560 2017 2265 2967 4463 560
Size: 1457 248 702 1496 683
Fragments arranged by size:
1496 1457 702 683 248
BsaWI w'CCGG_w
Cuts at: 981 1775 2296 2974 3248 3395 3806 981
Size: 794 521 678 274 147 411 1761
Fragments arranged by size:
1761 794 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 224 254 738 768 224
Size: 30 484 30 4042
Fragments arranged by size:
4042 484 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 471 471
Size: 4586
BseYI C'CCAG_C
Cuts at: 341 1500 2011 2769 3346 3842 341
Size: 1159 511 758 577 496 1085
Fragments arranged by size:
1159 1085 758 577 511 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 589
Size: 4586
BsiEI CG_ry'CG
Cuts at: 104 2128 2174 3382 104
Size: 2024 46 1208 1308
Fragments arranged by size:
2024 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 164 188 1600 2378 2568 3360 164
Size: 24 1412 778 190 792 1390
Fragments arranged by size:
1412 1390 792 778 190 24
BsmI GAATG_Cn'
Cuts at: 1560 1560
Size: 4586
BsmBI CGTCTCn'nnnn_
Cuts at: 741 741
Size: 4586
Bsp1286I G_dGCh'C
Cuts at: 164 171 180 188 401 555 1600 2211 2304
Size: 7 9 8 213 154 1045 611 93
Cuts at: 2304 2378 2568 2630 3360 164
Size: 74 190 62 730 1390
Fragments arranged by size:
1390 1045 730 611 213 190 154 93
74 62 9 8 7
BspEI T'CCGG_A
Cuts at: 3806 3806
Size: 4586
BspHI T'CATG_A
Cuts at: 1053 3762 1053
Size: 2709 1877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 224 1636 1850 2152 2533 224
Size: 1412 214 302 381 2277
Fragments arranged by size:
2277 1412 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2878 2932 4416 4581 50
Size: 2828 54 1484 165 55
Fragments arranged by size:
2828 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1256 1547 2498 1256
Size: 291 951 3344
Fragments arranged by size:
3344 951 291
BsrFI r'CCGG_y
Cuts at: 2584 2765 2974 2584
Size: 181 209 4196
Fragments arranged by size:
4196 209 181
BssSI C'ACGA_G
Cuts at: 2857 3215 2857
Size: 358 4228
BstAPI GCAn_nnn'nTGC
Cuts at: 1963 1963
Size: 4586
BstBI TT'CG_AA
Cuts at: 2947 2947
Size: 4586
BstYI r'GATC_y
Cuts at: 1 536 876 890 1161 1272 1611 2078 2101
Size: 535 340 14 271 111 339 467 23
Cuts at: 2101 2436 2682 3694 3780 3792 4138 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 535 467 449 346 340 339 335
271 246 111 86 23 14 12
BtgI C'CryG_G
Cuts at: 171 349 555 171
Size: 178 206 4202
Fragments arranged by size:
4202 206 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2536 2717 2880 2536
Size: 181 163 4242
Fragments arranged by size:
4242 181 163
BtsI GCAGTG_nn'
Cuts at: 284 1829 284
Size: 1545 3041
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 750 785 750
Size: 35 4551
DraI TTT'AAA
Cuts at: 791 1244 1585 791
Size: 453 341 3792
Fragments arranged by size:
3792 453 341
DrdI GACnn_nn'nnGTC
Cuts at: 2292 3150 2292
Size: 858 3728
EaeI y'GGCC_r
Cuts at: 155 1065 2171 2345 2736 2763 4108 155
Size: 910 1106 174 391 27 1345 633
Fragments arranged by size:
1345 1106 910 633 391 174 27
EagI C'GGCC_G
Cuts at: 2171 2171
Size: 4586
EarI CTCTTCn'nnn_
Cuts at: 325 1315 2609 2819 325
Size: 990 1294 210 2092
Fragments arranged by size:
2092 1294 990 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2794 3104 3250 4396 2794
Size: 310 146 1146 2984
Fragments arranged by size:
2984 1146 310 146
EcoICRI GAG'CTC
Cuts at: 162 1598 162
Size: 1436 3150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 857 1336 1536 2411 2843 3590 3858 202
Size: 655 479 200 875 432 747 268 930
Fragments arranged by size:
930 875 747 655 479 432 268 200
EcoNI CCTnn'n_nnAGG
Cuts at: 1126 1126
Size: 4586
EcoO109I rG'GnC_Cy
Cuts at: 176 177 1725 176
Size: 1 1548 3037
Fragments arranged by size:
3037 1548 1
EcoRI G'AATT_C
Cuts at: 1590 1590
Size: 4586
EcoRV GAT'ATC
Cuts at: 255 1134 255
Size: 879 3707
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1035 2605 1035
Size: 1570 3016
FspI TGC'GCA
Cuts at: 2367 2367
Size: 4586
HaeII r_GCGC'y
Cuts at: 145 576 2020 2268 3290 4269 145
Size: 431 1444 248 1022 979 462
Fragments arranged by size:
1444 1022 979 462 431 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 224 256 861 893 1030 1062 2428 2458 2460
Size: 32 605 32 137 32 1366 30 2
Cuts at: 2460 2490 224
Size: 30 2320
Fragments arranged by size:
2320 1366 605 137 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 683 1625 683
Size: 942 3644
HindIII A'AGCT_T
Cuts at: 1012 1012
Size: 4586
KasI G'GCGC_C
Cuts at: 2016 2264 2016
Size: 248 4338
KpnI G_GTAC'C
Cuts at: 1606 1606
Size: 4586
MluI A'CGCG_T
Cuts at: 4019 4019
Size: 4586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1222 3257 3441 1222
Size: 2035 184 2367
Fragments arranged by size:
2367 2035 184
MscI TGG'CCA
Cuts at: 2347 2347
Size: 4586
MslI CAynn'nnrTG
Cuts at: 1468 1517 2575 2702 1468
Size: 49 1058 127 3352
Fragments arranged by size:
3352 1058 127 49
MspA1I CmG'CkG
Cuts at: 150 194 1697 2011 2371 3384 3629 150
Size: 44 1503 314 360 1013 245 1107
Fragments arranged by size:
1503 1107 1013 360 314 245 44
NaeI GCC'GGC
Cuts at: 2767 2767
Size: 4586
NarI GG'CG_CC
Cuts at: 2017 2265 2017
Size: 248 4338
NcoI C'CATG_G
Cuts at: 555 555
Size: 4586
NgoMIV G'CCGG_C
Cuts at: 2765 2765
Size: 4586
NspI r_CATG'y
Cuts at: 1639 2139 3046 1639
Size: 500 907 3179
Fragments arranged by size:
3179 907 500
PasI CC'CwG_GG
Cuts at: 1109 1109
Size: 4586
PciI A'CATG_T
Cuts at: 3042 3042
Size: 4586
PflMI CCAn_nnn'nTGG
Cuts at: 1074 1204 1074
Size: 130 4456
PfoI T'CCnGG_A
Cuts at: 539 539
Size: 4586
PmeI GTTT'AAAC
Cuts at: 1585 1585
Size: 4586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 3725
Size: 32 4554
PsiI TTA'TAA
Cuts at: 1228 1228
Size: 4586
PspOMI G'GGCC_C
Cuts at: 176 176
Size: 4586
PstI C_TGCA'G
Cuts at: 149 1633 2318 149
Size: 1484 685 2417
Fragments arranged by size:
2417 1484 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4586
PvuII CAG'CTG
Cuts at: 150 194 2011 2371 150
Size: 44 1817 360 2365
Fragments arranged by size:
2365 1817 360 44
RsrII CG'GwC_CG
Cuts at: 2781 2781
Size: 4586
SacI G_AGCT'C
Cuts at: 164 1600 164
Size: 1436 3150
SalI G'TCGA_C
Cuts at: 1623 1623
Size: 4586
SapI GCTCTTCn'nnn_
Cuts at: 2609 2819 2609
Size: 210 4376
SbfI CC_TGCA'GG
Cuts at: 1633 1633
Size: 4586
ScaI AGT'ACT
Cuts at: 4103 4103
Size: 4586
SfcI C'TryA_G
Cuts at: 33 145 1629 2314 3307 3498 33
Size: 112 1484 685 993 191 1121
Fragments arranged by size:
1484 1121 993 685 191 112
SfoI GGC'GCC
Cuts at: 2018 2266 2018
Size: 248 4338
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
SmaI CCC'GGG
Cuts at: 114 1608 114
Size: 1494 3092
SmlI C'TyrA_G
Cuts at: 1741 2656 3148 3410 1741
Size: 915 492 262 2917
Fragments arranged by size:
2917 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4586
SphI G_CATG'C
Cuts at: 1639 2139 1639
Size: 500 4086
StuI AGG'CCT
Cuts at: 365 365
Size: 4586
StyI C'CwwG_G
Cuts at: 555 854 1720 2074 555
Size: 299 866 354 3067
Fragments arranged by size:
3067 866 354 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 541 2169 2302 2761 2970 541
Size: 1628 133 459 209 2157
Fragments arranged by size:
2157 1628 459 209 133
TatI w'GTAC_w
Cuts at: 4101 4101
Size: 4586
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1338 1370 1546 1578 4103 4135 1338
Size: 32 176 32 2525 32 1789
Fragments arranged by size:
2525 1789 176 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2383 2383
Size: 4586
XbaI T'CTAG_A
Cuts at: 1617 1617
Size: 4586
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1155 1428 2081 1155
Size: 273 653 3660
Fragments arranged by size:
3660 653 273
XmaI C'CCGG_G
Cuts at: 112 1606 112
Size: 1494 3092
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PpuMI PshAI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI