Restriction map for
pF1KB5858
(Circular) (Six-base) MAPSORT of: pF1KB5858.seq Check: 9569 from: 1 to: 4583
pF1KB5858 4583 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 277 346 277
Size: 69 4514
AccI GT'mk_AC
Cuts at: 1621 1621
Size: 4583
Acc65I G'GTAC_C
Cuts at: 1599 1599
Size: 4583
AclI AA'CG_TT
Cuts at: 878 4423 878
Size: 3545 1038
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 925 976 2408 2840 3587 3855 190
Size: 735 51 1432 432 747 268 918
Fragments arranged by size:
1432 918 747 735 432 268 51
AflIII A'CryG_T
Cuts at: 931 1935 3039 4016 931
Size: 1004 1104 977 1498
Fragments arranged by size:
1498 1104 1004 977
AgeI A'CCGG_T
Cuts at: 2971 2971
Size: 4583
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1204 1236 1204
Size: 32 4551
AlwNI CAG_nnn'CTG
Cuts at: 1888 3455 1888
Size: 1567 3016
ApaI G_GGCC'C
Cuts at: 822 822
Size: 4583
ApaLI G'TGCA_C
Cuts at: 3353 3353
Size: 4583
ApoI r'AATT_y
Cuts at: 1471 1587 3936 4192 1471
Size: 116 2349 256 1862
Fragments arranged by size:
2349 1862 256 116
AseI AT'TA_AT
Cuts at: 20 20
Size: 4583
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
AvaI C'yCGr_G
Cuts at: 1603 1603
Size: 4583
AvrII C'CTAG_G
Cuts at: 822 822
Size: 4583
BamHI G'GATC_C
Cuts at: 1608 1608
Size: 4583
BanI G'GyrC_C
Cuts at: 402 460 1599 2013 2261 2296 402
Size: 58 1139 414 248 35 2689
Fragments arranged by size:
2689 1139 414 248 58 35
BanII G_rGCy'C
Cuts at: 470 822 1538 1597 2627 470
Size: 352 716 59 1030 2426
Fragments arranged by size:
2426 1030 716 352 59
BbeI G_GCGC'C
Cuts at: 2017 2265 2017
Size: 248 4335
BbsI GAAGACnn'nnnn_
Cuts at: 132 132
Size: 4583
BbvCI CC'TCA_GC
Cuts at: 149 149
Size: 4583
BciVI GTATCCnnnnn_n'
Cuts at: 1032 2481 3242 4583 1032
Size: 1449 761 1341 1032
Fragments arranged by size:
1449 1341 1032 761
BclI T'GATC_A
Cuts at: 663 2103 663
Size: 1440 3143
BglII A'GATC_T
Cuts at: 830 2098 4135 830
Size: 1268 2037 1278
Fragments arranged by size:
2037 1278 1268
BlpI GC'TnA_GC
Cuts at: 212 1168 1695 212
Size: 956 527 3100
Fragments arranged by size:
3100 956 527
Bme1580I G_kGCm'C
Cuts at: 407 822 1346 2208 2301 3357 407
Size: 415 524 862 93 1056 1633
Fragments arranged by size:
1633 1056 862 524 415 93
BmgBI CAC'GTC
Cuts at: 558 558
Size: 4583
BmrI ACTGGGnnnn_n'
Cuts at: 1291 1795 1876 2211 4355 1291
Size: 504 81 335 2144 1519
Fragments arranged by size:
2144 1519 504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1233 2950 1233
Size: 1717 2866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 1183 1135
Size: 48 4535
Bpu10I CC'TnA_GC
Cuts at: 149 149
Size: 4583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1759 2638 3130 3428 1759
Size: 879 492 298 2914
Fragments arranged by size:
2914 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4287 4287
Size: 4583
BsaAI yAC'GTr
Cuts at: 513 1936 513
Size: 1423 3160
BsaBI GATnn'nnATC
Cuts at: 6 2097 4134 6
Size: 2091 2037 455
Fragments arranged by size:
2091 2037 455
BsaHI Gr'CG_yC
Cuts at: 2014 2262 2964 4460 2014
Size: 248 702 1496 2137
Fragments arranged by size:
2137 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1772 2293 2971 3245 3392 3803 1772
Size: 521 678 274 147 411 2552
Fragments arranged by size:
2552 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1117 1147 1204 1234 1378 1408 1117
Size: 30 57 30 144 30 4292
Fragments arranged by size:
4292 144 57 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 544 547 859 1225 544
Size: 3 312 366 3902
Fragments arranged by size:
3902 366 312 3
BseYI C'CCAG_C
Cuts at: 1537 1554 2008 2766 3343 3839 1537
Size: 17 454 758 577 496 2281
Fragments arranged by size:
2281 758 577 496 454 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 912 1453 912
Size: 541 4042
BsiEI CG_ry'CG
Cuts at: 104 333 2125 2171 3379 104
Size: 229 1792 46 1208 1308
Fragments arranged by size:
1792 1308 1208 229 46
BsiHKAI G_wGCw'C
Cuts at: 521 1597 2375 2565 3357 521
Size: 1076 778 190 792 1747
Fragments arranged by size:
1747 1076 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 407 470 521 822 1346 1538 1597 2208 2301
Size: 63 51 301 524 192 59 611 93
Cuts at: 2301 2375 2565 2627 3357 407
Size: 74 190 62 730 1633
Fragments arranged by size:
1633 730 611 524 301 192 190 93
74 63 62 59 51
BspEI T'CCGG_A
Cuts at: 3803 3803
Size: 4583
BspHI T'CATG_A
Cuts at: 768 1083 3759 768
Size: 315 2676 1592
Fragments arranged by size:
2676 1592 315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 277 346 554 1512 1633 1847 2149 2530 277
Size: 69 208 958 121 214 302 381 2330
Fragments arranged by size:
2330 958 381 302 214 208 121 69
BsrBI CCG'CTC
Cuts at: 50 216 2875 2929 4413 4578 50
Size: 166 2659 54 1484 165 55
Fragments arranged by size:
2659 1484 166 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1548 2495 1548
Size: 947 3636
BsrFI r'CCGG_y
Cuts at: 2581 2762 2971 2581
Size: 181 209 4193
Fragments arranged by size:
4193 209 181
BssHII G'CGCG_C
Cuts at: 430 430
Size: 4583
BssSI C'ACGA_G
Cuts at: 2854 3212 2854
Size: 358 4225
BstAPI GCAn_nnn'nTGC
Cuts at: 1960 1960
Size: 4583
BstBI TT'CG_AA
Cuts at: 2944 2944
Size: 4583
BstXI CCAn_nnnn'nTGG
Cuts at: 301 1236 1377 301
Size: 935 141 3507
Fragments arranged by size:
3507 935 141
BstYI r'GATC_y
Cuts at: 1 830 1608 2075 2098 2433 2679 3691 3777
Size: 829 778 467 23 335 246 1012 86
Cuts at: 3777 3789 4135 1
Size: 12 346 449
Fragments arranged by size:
1012 829 778 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 491 683 491
Size: 192 4391
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 280 2533 2714 2877 280
Size: 2253 181 163 1986
Fragments arranged by size:
2253 1986 181 163
BtsI GCAGTG_nn'
Cuts at: 236 313 782 939 1826 236
Size: 77 469 157 887 2993
Fragments arranged by size:
2993 887 469 157 77
DraI TTT'AAA
Cuts at: 1582 1582
Size: 4583
DrdI GACnn_nn'nnGTC
Cuts at: 2289 3147 2289
Size: 858 3725
EaeI y'GGCC_r
Cuts at: 380 580 1289 1529 2168 2342 2733 2760 4105
Size: 200 709 240 639 174 391 27 1345
Cuts at: 4105 380
Size: 858
Fragments arranged by size:
1345 858 709 639 391 240 200 174
27
EagI C'GGCC_G
Cuts at: 2168 2168
Size: 4583
EarI CTCTTCn'nnn_
Cuts at: 973 2606 2816 973
Size: 1633 210 2740
Fragments arranged by size:
2740 1633 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1450 2791 3101 3247 4393 1450
Size: 1341 310 146 1146 1640
Fragments arranged by size:
1640 1341 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1595 1595
Size: 4583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 925 976 1135 1183 2408 2840 3587 3855
Size: 735 51 159 48 1225 432 747 268
Cuts at: 3855 190
Size: 918
Fragments arranged by size:
1225 918 747 735 432 268 159 51
48
EcoO109I rG'GnC_Cy
Cuts at: 543 819 1185 1722 543
Size: 276 366 537 3404
Fragments arranged by size:
3404 537 366 276
EcoRI G'AATT_C
Cuts at: 1471 1587 1471
Size: 116 4467
EcoRV GAT'ATC
Cuts at: 201 201
Size: 4583
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 4583
FspI TGC'GCA
Cuts at: 2364 2364
Size: 4583
HaeII r_GCGC'y
Cuts at: 2017 2265 3287 4266 2017
Size: 248 1022 979 2334
Fragments arranged by size:
2334 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2425 2455 2457 2487 2425
Size: 30 2 30 4521
Fragments arranged by size:
4521 30 30 2
HincII GTy'rAC
Cuts at: 1622 1622
Size: 4583
KasI G'GCGC_C
Cuts at: 2013 2261 2013
Size: 248 4335
KpnI G_GTAC'C
Cuts at: 1603 1603
Size: 4583
MluI A'CGCG_T
Cuts at: 4016 4016
Size: 4583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3254 3438 3254
Size: 184 4399
MscI TGG'CCA
Cuts at: 2344 2344
Size: 4583
MslI CAynn'nnrTG
Cuts at: 299 368 1153 1291 1336 1429 2572 2699 299
Size: 69 785 138 45 93 1143 127 2183
Fragments arranged by size:
2183 1143 785 138 127 93 69 45
MspA1I CmG'CkG
Cuts at: 148 903 1694 2008 2368 3381 3626 148
Size: 755 791 314 360 1013 245 1105
Fragments arranged by size:
1105 1013 791 755 360 314 245
NaeI GCC'GGC
Cuts at: 2764 2764
Size: 4583
NarI GG'CG_CC
Cuts at: 2014 2262 2014
Size: 248 4335
NgoMIV G'CCGG_C
Cuts at: 2762 2762
Size: 4583
NspI r_CATG'y
Cuts at: 609 935 1158 1324 1458 1636 2136 3043 609
Size: 326 223 166 134 178 500 907 2149
Fragments arranged by size:
2149 907 500 326 223 178 166 134
PciI A'CATG_T
Cuts at: 931 3039 931
Size: 2108 2475
PmeI GTTT'AAAC
Cuts at: 1582 1582
Size: 4583
PmlI CAC'GTG
Cuts at: 513 513
Size: 4583
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1204 1236 3722 3754 1204
Size: 32 2486 32 2033
Fragments arranged by size:
2486 2033 32 32
PpuMI rG'GwC_Cy
Cuts at: 543 1185 543
Size: 642 3941
PspOMI G'GGCC_C
Cuts at: 818 818
Size: 4583
PstI C_TGCA'G
Cuts at: 198 680 1630 2315 198
Size: 482 950 685 2466
Fragments arranged by size:
2466 950 685 482
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4583
PvuII CAG'CTG
Cuts at: 903 2008 2368 903
Size: 1105 360 3118
Fragments arranged by size:
3118 1105 360
RsrII CG'GwC_CG
Cuts at: 2778 2778
Size: 4583
SacI G_AGCT'C
Cuts at: 1597 1597
Size: 4583
SalI G'TCGA_C
Cuts at: 1620 1620
Size: 4583
SapI GCTCTTCn'nnn_
Cuts at: 2606 2816 2606
Size: 210 4373
SbfI CC_TGCA'GG
Cuts at: 680 1630 680
Size: 950 3633
ScaI AGT'ACT
Cuts at: 577 916 4100 577
Size: 339 3184 1060
Fragments arranged by size:
3184 1060 339
SfcI C'TryA_G
Cuts at: 33 194 676 1626 2311 3304 3495 33
Size: 161 482 950 685 993 191 1121
Fragments arranged by size:
1121 993 950 685 482 191 161
SfoI GGC'GCC
Cuts at: 2015 2263 2015
Size: 248 4335
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4583
SmaI CCC'GGG
Cuts at: 1605 1605
Size: 4583
SmlI C'TyrA_G
Cuts at: 1738 2653 3145 3407 1738
Size: 915 492 262 2914
Fragments arranged by size:
2914 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4583
SphI G_CATG'C
Cuts at: 609 1324 1636 2136 609
Size: 715 312 500 3056
Fragments arranged by size:
3056 715 500 312
StuI AGG'CCT
Cuts at: 674 1204 1216 674
Size: 530 12 4041
Fragments arranged by size:
4041 530 12
StyI C'CwwG_G
Cuts at: 179 822 1034 1717 2071 179
Size: 643 212 683 354 2691
Fragments arranged by size:
2691 683 643 354 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2166 2299 2758 2967 2166
Size: 133 459 209 3782
Fragments arranged by size:
3782 459 209 133
TatI w'GTAC_w
Cuts at: 575 611 742 914 1478 4098 575
Size: 36 131 172 564 2620 1060
Fragments arranged by size:
2620 1060 564 172 131 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1378 1410 4100 4132 1378
Size: 32 2690 32 1829
Fragments arranged by size:
2690 1829 32 32
Tth111I GACn'n_nGTC
Cuts at: 855 2380 855
Size: 1525 3058
XbaI T'CTAG_A
Cuts at: 1614 1614
Size: 4583
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 711 1041 1236 1524 2078 711
Size: 330 195 288 554 3216
Fragments arranged by size:
3216 554 330 288 195
XmaI C'CCGG_G
Cuts at: 1603 1603
Size: 4583
XmnI GAAnn'nnTTC
Cuts at: 1471 1471
Size: 4583
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI BaeI
BcgI BfrBI BglI BmtI BsiWI BsmI BsmBI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI