Restriction map for
pF1KB5842
(Circular) (Six-base) MAPSORT of: pF1KB5842.seq Check: 2710 from: 1 to: 4562
pF1KB5842 4562 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 1600 1600
Size: 4562
Acc65I G'GTAC_C
Cuts at: 1578 1578
Size: 4562
AclI AA'CG_TT
Cuts at: 4402 4402
Size: 4562
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1045 1087 2387 2819 3566 3834 607
Size: 438 42 1300 432 747 268 1335
Fragments arranged by size:
1335 1300 747 438 432 268 42
AflIII A'CryG_T
Cuts at: 1914 3018 3995 1914
Size: 1104 977 2481
Fragments arranged by size:
2481 1104 977
AgeI A'CCGG_T
Cuts at: 2950 2950
Size: 4562
AlwNI CAG_nnn'CTG
Cuts at: 1867 3434 1867
Size: 1567 2995
ApaLI G'TGCA_C
Cuts at: 754 3332 754
Size: 2578 1984
ApoI r'AATT_y
Cuts at: 437 653 972 1566 3915 4171 437
Size: 216 319 594 2349 256 828
Fragments arranged by size:
2349 828 594 319 256 216
AseI AT'TA_AT
Cuts at: 20 20
Size: 4562
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4562
AvaI C'yCGr_G
Cuts at: 1292 1582 1292
Size: 290 4272
BamHI G'GATC_C
Cuts at: 1587 1587
Size: 4562
BanI G'GyrC_C
Cuts at: 550 1578 1992 2240 2275 550
Size: 1028 414 248 35 2837
Fragments arranged by size:
2837 1028 414 248 35
BanII G_rGCy'C
Cuts at: 1576 2606 1576
Size: 1030 3532
BbeI G_GCGC'C
Cuts at: 1996 2244 1996
Size: 248 4314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1204 1238 1204
Size: 34 4528
BciVI GTATCCnnnnn_n'
Cuts at: 2460 3221 4562 2460
Size: 761 1341 2460
Fragments arranged by size:
2460 1341 761
BclI T'GATC_A
Cuts at: 819 1418 2082 819
Size: 599 664 3299
Fragments arranged by size:
3299 664 599
BglII A'GATC_T
Cuts at: 2077 4114 2077
Size: 2037 2525
BlpI GC'TnA_GC
Cuts at: 1404 1674 1404
Size: 270 4292
Bme1580I G_kGCm'C
Cuts at: 214 758 2187 2280 3336 214
Size: 544 1429 93 1056 1440
Fragments arranged by size:
1440 1429 1056 544 93
BmrI ACTGGGnnnn_n'
Cuts at: 1032 1186 1774 1855 2190 4334 1032
Size: 154 588 81 335 2144 1260
Fragments arranged by size:
2144 1260 588 335 154 81
BmtI G_CTAG'C
Cuts at: 1159 1159
Size: 4562
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2929 2929
Size: 4562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 1080 221
Size: 859 3703
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1738 2617 3109 3407 1738
Size: 879 492 298 2893
Fragments arranged by size:
2893 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1348 4266 1348
Size: 2918 1644
BsaAI yAC'GTr
Cuts at: 759 1915 759
Size: 1156 3406
BsaBI GATnn'nnATC
Cuts at: 6 2076 4113 6
Size: 2070 2037 455
Fragments arranged by size:
2070 2037 455
BsaHI Gr'CG_yC
Cuts at: 1993 2241 2943 4439 1993
Size: 248 702 1496 2116
Fragments arranged by size:
2116 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1751 2272 2950 3224 3371 3782 1751
Size: 521 678 274 147 411 2531
Fragments arranged by size:
2531 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 218 493 1125 1471 218
Size: 275 632 346 3309
Fragments arranged by size:
3309 632 346 275
BseYI C'CCAG_C
Cuts at: 452 1464 1987 2745 3322 3818 452
Size: 1012 523 758 577 496 1196
Fragments arranged by size:
1196 1012 758 577 523 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 898 898
Size: 4562
BsiEI CG_ry'CG
Cuts at: 104 2104 2150 3358 104
Size: 2000 46 1208 1308
Fragments arranged by size:
2000 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 115 758 1576 2354 2544 3336 115
Size: 643 818 778 190 792 1341
Fragments arranged by size:
1341 818 792 778 643 190
BsmI GAATG_Cn'
Cuts at: 485 485
Size: 4562
Bsp1286I G_dGCh'C
Cuts at: 115 214 758 1576 2187 2280 2354 2544 2606
Size: 99 544 818 611 93 74 190 62
Cuts at: 2606 3336 115
Size: 730 1341
Fragments arranged by size:
1341 818 730 611 544 190 99 93
74 62
BspEI T'CCGG_A
Cuts at: 3782 3782
Size: 4562
BspHI T'CATG_A
Cuts at: 968 1239 3738 968
Size: 271 2499 1792
Fragments arranged by size:
2499 1792 271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1612 1826 2128 2509 1612
Size: 214 302 381 3665
Fragments arranged by size:
3665 381 302 214
BsrBI CCG'CTC
Cuts at: 50 548 2854 2908 4392 4557 50
Size: 498 2306 54 1484 165 55
Fragments arranged by size:
2306 1484 498 165 55 54
BsrDI GCAATG_nn'
Cuts at: 491 614 2474 491
Size: 123 1860 2579
Fragments arranged by size:
2579 1860 123
BsrFI r'CCGG_y
Cuts at: 2560 2741 2950 2560
Size: 181 209 4172
Fragments arranged by size:
4172 209 181
BsrGI T'GTAC_A
Cuts at: 957 957
Size: 4562
BssSI C'ACGA_G
Cuts at: 2833 3191 2833
Size: 358 4204
BstAPI GCAn_nnn'nTGC
Cuts at: 1939 1939
Size: 4562
BstBI TT'CG_AA
Cuts at: 2923 2923
Size: 4562
BstXI CCAn_nnnn'nTGG
Cuts at: 696 1372 696
Size: 676 3886
BstYI r'GATC_y
Cuts at: 1 351 789 1587 2054 2077 2412 2658 3670
Size: 350 438 798 467 23 335 246 1012
Cuts at: 3670 3756 3768 4114 1
Size: 86 12 346 449
Fragments arranged by size:
1012 798 467 449 438 350 346 335
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2512 2693 2856 2512
Size: 181 163 4218
Fragments arranged by size:
4218 181 163
BtsI GCAGTG_nn'
Cuts at: 1074 1805 1074
Size: 731 3831
DraI TTT'AAA
Cuts at: 335 1561 335
Size: 1226 3336
DraIII CAC_nnn'GTG
Cuts at: 762 762
Size: 4562
DrdI GACnn_nn'nnGTC
Cuts at: 2268 3126 2268
Size: 858 3704
EaeI y'GGCC_r
Cuts at: 116 2147 2321 2712 2739 4084 116
Size: 2031 174 391 27 1345 594
Fragments arranged by size:
2031 1345 594 391 174 27
EagI C'GGCC_G
Cuts at: 2147 2147
Size: 4562
EarI CTCTTCn'nnn_
Cuts at: 2585 2795 2585
Size: 210 4352
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2770 3080 3226 4372 2770
Size: 310 146 1146 2960
Fragments arranged by size:
2960 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1574 1574
Size: 4562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 607 1045 1080 1087 2387 2819 3566 3834
Size: 386 438 35 7 1300 432 747 268
Cuts at: 3834 221
Size: 949
Fragments arranged by size:
1300 949 747 438 432 386 268 35
7
EcoNI CCTnn'n_nnAGG
Cuts at: 203 589 1009 203
Size: 386 420 3756
Fragments arranged by size:
3756 420 386
EcoO109I rG'GnC_Cy
Cuts at: 175 1701 175
Size: 1526 3036
EcoRI G'AATT_C
Cuts at: 972 1566 972
Size: 594 3968
EcoRV GAT'ATC
Cuts at: 513 513
Size: 4562
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 4562
FspI TGC'GCA
Cuts at: 2343 2343
Size: 4562
HaeII r_GCGC'y
Cuts at: 1065 1996 2244 3266 4245 1065
Size: 931 248 1022 979 1382
Fragments arranged by size:
1382 1022 979 931 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 364 396 2404 2434 2436 2466 364
Size: 32 2008 30 2 30 2460
Fragments arranged by size:
2460 2008 32 30 30 2
HincII GTy'rAC
Cuts at: 1601 1601
Size: 4562
KasI G'GCGC_C
Cuts at: 1992 2240 1992
Size: 248 4314
KpnI G_GTAC'C
Cuts at: 1582 1582
Size: 4562
MluI A'CGCG_T
Cuts at: 3995 3995
Size: 4562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3233 3417 3233
Size: 184 4378
MscI TGG'CCA
Cuts at: 2323 2323
Size: 4562
MslI CAynn'nnrTG
Cuts at: 2551 2678 2551
Size: 127 4435
MspA1I CmG'CkG
Cuts at: 168 240 1464 1673 1987 2347 3360 3605 168
Size: 72 1224 209 314 360 1013 245 1125
Fragments arranged by size:
1224 1125 1013 360 314 245 209 72
NaeI GCC'GGC
Cuts at: 2743 2743
Size: 4562
NarI GG'CG_CC
Cuts at: 1993 2241 1993
Size: 248 4314
NdeI CA'TA_TG
Cuts at: 1050 1425 1050
Size: 375 4187
NgoMIV G'CCGG_C
Cuts at: 2741 2741
Size: 4562
NheI G'CTAG_C
Cuts at: 1155 1155
Size: 4562
NspI r_CATG'y
Cuts at: 1615 2115 3022 1615
Size: 500 907 3155
Fragments arranged by size:
3155 907 500
PasI CC'CwG_GG
Cuts at: 775 775
Size: 4562
PciI A'CATG_T
Cuts at: 3018 3018
Size: 4562
PfoI T'CCnGG_A
Cuts at: 785 785
Size: 4562
PmeI GTTT'AAAC
Cuts at: 1561 1561
Size: 4562
PmlI CAC'GTG
Cuts at: 759 759
Size: 4562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 3701
Size: 32 4530
PsiI TTA'TAA
Cuts at: 139 139
Size: 4562
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 623 655 623
Size: 32 4530
PstI C_TGCA'G
Cuts at: 1609 2294 1609
Size: 685 3877
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4562
PvuII CAG'CTG
Cuts at: 168 240 1464 1987 2347 168
Size: 72 1224 523 360 2383
Fragments arranged by size:
2383 1224 523 360 72
RsrII CG'GwC_CG
Cuts at: 2757 2757
Size: 4562
SacI G_AGCT'C
Cuts at: 1576 1576
Size: 4562
SalI G'TCGA_C
Cuts at: 1599 1599
Size: 4562
SapI GCTCTTCn'nnn_
Cuts at: 2585 2795 2585
Size: 210 4352
SbfI CC_TGCA'GG
Cuts at: 1609 1609
Size: 4562
ScaI AGT'ACT
Cuts at: 1042 4079 1042
Size: 3037 1525
SexAI A'CCwGG_T
Cuts at: 309 309
Size: 4562
SfcI C'TryA_G
Cuts at: 33 640 1538 1605 2290 3283 3474 33
Size: 607 898 67 685 993 191 1121
Fragments arranged by size:
1121 993 898 685 607 191 67
SfoI GGC'GCC
Cuts at: 1994 2242 1994
Size: 248 4314
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4562
SmaI CCC'GGG
Cuts at: 1584 1584
Size: 4562
SmlI C'TyrA_G
Cuts at: 1717 2632 3124 3386 1717
Size: 915 492 262 2893
Fragments arranged by size:
2893 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4562
SphI G_CATG'C
Cuts at: 1615 2115 1615
Size: 500 4062
StyI C'CwwG_G
Cuts at: 1696 2050 1696
Size: 354 4208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2145 2278 2737 2946 2145
Size: 133 459 209 3761
Fragments arranged by size:
3761 459 209 133
TatI w'GTAC_w
Cuts at: 957 1040 1071 4077 957
Size: 83 31 3006 1442
Fragments arranged by size:
3006 1442 83 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4079 4111 4079
Size: 32 4530
Tth111I GACn'n_nGTC
Cuts at: 2359 2359
Size: 4562
XbaI T'CTAG_A
Cuts at: 1593 1593
Size: 4562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2057 2057
Size: 4562
XmaI C'CCGG_G
Cuts at: 1582 1582
Size: 4562
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NheI NspI PasI PciI PfoI PmeI
PmlI PpiI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI Bpu10I BsaXI BsiWI BsmBI BssHII BstEII BstZ17I
Bsu36I BtgI ClaI CspCI FseI FspAI HindIII HpaI
MfeI NcoI NotI NruI NsiI PacI PflMI PpuMI
PshAI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI