Restriction map for
pF1KB5840
(Circular) (Six-base) MAPSORT of: pF1KB5840.seq Check: 4955 from: 1 to: 4559
pF1KB5840 4559 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 295 1459 295
Size: 1164 3395
AccI GT'mk_AC
Cuts at: 1597 1597
Size: 4559
Acc65I G'GTAC_C
Cuts at: 1575 1575
Size: 4559
AclI AA'CG_TT
Cuts at: 4399 4399
Size: 4559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 1517 2384 2816 3563 3831 689
Size: 828 867 432 747 268 1417
Fragments arranged by size:
1417 867 828 747 432 268
AflIII A'CryG_T
Cuts at: 818 1911 3015 3992 818
Size: 1093 1104 977 1385
Fragments arranged by size:
1385 1104 1093 977
AgeI A'CCGG_T
Cuts at: 2947 2947
Size: 4559
AlwNI CAG_nnn'CTG
Cuts at: 837 1495 1864 3431 837
Size: 658 369 1567 1965
Fragments arranged by size:
1965 1567 658 369
ApaI G_GGCC'C
Cuts at: 1347 1347
Size: 4559
ApaLI G'TGCA_C
Cuts at: 3329 3329
Size: 4559
ApoI r'AATT_y
Cuts at: 564 1563 3912 4168 564
Size: 999 2349 256 955
Fragments arranged by size:
2349 999 955 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4559
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
AvaI C'yCGr_G
Cuts at: 1579 1579
Size: 4559
BamHI G'GATC_C
Cuts at: 1007 1584 1007
Size: 577 3982
BanI G'GyrC_C
Cuts at: 1575 1989 2237 2272 1575
Size: 414 248 35 3862
Fragments arranged by size:
3862 414 248 35
BanII G_rGCy'C
Cuts at: 688 914 1347 1573 2603 688
Size: 226 433 226 1030 2644
Fragments arranged by size:
2644 1030 433 226 226
BbeI G_GCGC'C
Cuts at: 1993 2241 1993
Size: 248 4311
BciVI GTATCCnnnnn_n'
Cuts at: 2457 3218 4559 2457
Size: 761 1341 2457
Fragments arranged by size:
2457 1341 761
BclI T'GATC_A
Cuts at: 2079 2079
Size: 4559
BfrBI ATG'CAT
Cuts at: 303 303
Size: 4559
BglII A'GATC_T
Cuts at: 2074 4111 2074
Size: 2037 2522
BlpI GC'TnA_GC
Cuts at: 1067 1671 1067
Size: 604 3955
Bme1580I G_kGCm'C
Cuts at: 751 1347 2184 2277 3333 751
Size: 596 837 93 1056 1977
Fragments arranged by size:
1977 1056 837 596 93
BmgBI CAC'GTC
Cuts at: 1237 1237
Size: 4559
BmrI ACTGGGnnnn_n'
Cuts at: 612 983 1155 1771 1852 2187 4331 612
Size: 371 172 616 81 335 2144 840
Fragments arranged by size:
2144 840 616 371 335 172 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2926 2926
Size: 4559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 486 1024 1735 2614 3106 3404 486
Size: 538 711 879 492 298 1641
Fragments arranged by size:
1641 879 711 538 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 404 4263 404
Size: 3859 700
BsaAI yAC'GTr
Cuts at: 1912 1912
Size: 4559
BsaBI GATnn'nnATC
Cuts at: 6 2073 4110 6
Size: 2067 2037 455
Fragments arranged by size:
2067 2037 455
BsaHI Gr'CG_yC
Cuts at: 1990 2238 2940 4436 1990
Size: 248 702 1496 2113
Fragments arranged by size:
2113 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1748 2269 2947 3221 3368 3779 1748
Size: 521 678 274 147 411 2528
Fragments arranged by size:
2528 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1074 1168 1315 1074
Size: 94 147 4318
Fragments arranged by size:
4318 147 94
BseYI C'CCAG_C
Cuts at: 1437 1984 2742 3319 3815 1437
Size: 547 758 577 496 2181
Fragments arranged by size:
2181 758 577 547 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 648 946 1236 648
Size: 298 290 3971
Fragments arranged by size:
3971 298 290
BsiEI CG_ry'CG
Cuts at: 104 2101 2147 3355 104
Size: 1997 46 1208 1308
Fragments arranged by size:
1997 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 914 1573 2351 2541 3333 914
Size: 659 778 190 792 2140
Fragments arranged by size:
2140 792 778 659 190
BsmI GAATG_Cn'
Cuts at: 615 1046 1111 615
Size: 431 65 4063
Fragments arranged by size:
4063 431 65
Bsp1286I G_dGCh'C
Cuts at: 688 751 914 1347 1573 2184 2277 2351 2541
Size: 63 163 433 226 611 93 74 190
Cuts at: 2541 2603 3333 688
Size: 62 730 1914
Fragments arranged by size:
1914 730 611 433 226 190 163 93
74 63 62
BspEI T'CCGG_A
Cuts at: 3779 3779
Size: 4559
BspHI T'CATG_A
Cuts at: 1518 3735 1518
Size: 2217 2342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 281 295 580 1208 1459 1609 1823 2125 2506
Size: 14 285 628 251 150 214 302 381
Cuts at: 2506 281
Size: 2334
Fragments arranged by size:
2334 628 381 302 285 251 214 150
14
BsrBI CCG'CTC
Cuts at: 50 160 2851 2905 4389 4554 50
Size: 110 2691 54 1484 165 55
Fragments arranged by size:
2691 1484 165 110 55 54
BsrDI GCAATG_nn'
Cuts at: 1205 2471 1205
Size: 1266 3293
BsrFI r'CCGG_y
Cuts at: 2557 2738 2947 2557
Size: 181 209 4169
Fragments arranged by size:
4169 209 181
BssSI C'ACGA_G
Cuts at: 2830 3188 2830
Size: 358 4201
BstAPI GCAn_nnn'nTGC
Cuts at: 837 1936 837
Size: 1099 3460
BstBI TT'CG_AA
Cuts at: 2920 2920
Size: 4559
BstEII G'GTnAC_C
Cuts at: 282 282
Size: 4559
BstYI r'GATC_y
Cuts at: 1 953 1007 1548 1584 2051 2074 2409 2655
Size: 952 54 541 36 467 23 335 246
Cuts at: 2655 3667 3753 3765 4111 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 952 541 467 449 346 335 246
86 54 36 23 12
BtgI C'CryG_G
Cuts at: 107 725 801 107
Size: 618 76 3865
Fragments arranged by size:
3865 618 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2509 2690 2853 2509
Size: 181 163 4215
Fragments arranged by size:
4215 181 163
BtsI GCAGTG_nn'
Cuts at: 767 1802 767
Size: 1035 3524
ClaI AT'CG_AT
Cuts at: 514 514
Size: 4559
DraI TTT'AAA
Cuts at: 1558 1558
Size: 4559
DrdI GACnn_nn'nnGTC
Cuts at: 2265 3123 2265
Size: 858 3701
EaeI y'GGCC_r
Cuts at: 645 1290 2144 2318 2709 2736 4081 645
Size: 645 854 174 391 27 1345 1123
Fragments arranged by size:
1345 1123 854 645 391 174 27
EagI C'GGCC_G
Cuts at: 2144 2144
Size: 4559
EarI CTCTTCn'nnn_
Cuts at: 1157 1412 2582 2792 1157
Size: 255 1170 210 2924
Fragments arranged by size:
2924 1170 255 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 248 2767 3077 3223 4369 127
Size: 121 2519 310 146 1146 317
Fragments arranged by size:
2519 1146 317 310 146 121
EcoICRI GAG'CTC
Cuts at: 912 1571 912
Size: 659 3900
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 689 1517 2384 2816 3563 3831 689
Size: 828 867 432 747 268 1417
Fragments arranged by size:
1417 867 828 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1698 1698
Size: 4559
EcoRI G'AATT_C
Cuts at: 1563 1563
Size: 4559
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 4559
FspI TGC'GCA
Cuts at: 2340 2340
Size: 4559
HaeII r_GCGC'y
Cuts at: 999 1993 2241 3263 4242 999
Size: 994 248 1022 979 1316
Fragments arranged by size:
1316 1022 994 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2401 2431 2433 2463 2401
Size: 30 2 30 4497
Fragments arranged by size:
4497 30 30 2
HincII GTy'rAC
Cuts at: 1598 1598
Size: 4559
KasI G'GCGC_C
Cuts at: 1989 2237 1989
Size: 248 4311
KpnI G_GTAC'C
Cuts at: 1579 1579
Size: 4559
MluI A'CGCG_T
Cuts at: 3992 3992
Size: 4559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 501 1040 3230 3414 501
Size: 539 2190 184 1646
Fragments arranged by size:
2190 1646 539 184
MscI TGG'CCA
Cuts at: 2320 2320
Size: 4559
MslI CAynn'nnrTG
Cuts at: 964 2548 2675 964
Size: 1584 127 2848
Fragments arranged by size:
2848 1584 127
MspA1I CmG'CkG
Cuts at: 166 260 416 744 1670 1984 2344 3357 3602
Size: 94 156 328 926 314 360 1013 245
Cuts at: 3602 166
Size: 1123
Fragments arranged by size:
1123 1013 926 360 328 314 245 156
94
NaeI GCC'GGC
Cuts at: 2740 2740
Size: 4559
NarI GG'CG_CC
Cuts at: 1990 2238 1990
Size: 248 4311
NcoI C'CATG_G
Cuts at: 107 107
Size: 4559
NgoMIV G'CCGG_C
Cuts at: 2738 2738
Size: 4559
NsiI A_TGCA'T
Cuts at: 305 305
Size: 4559
NspI r_CATG'y
Cuts at: 822 1279 1612 2112 3019 822
Size: 457 333 500 907 2362
Fragments arranged by size:
2362 907 500 457 333
PasI CC'CwG_GG
Cuts at: 222 222
Size: 4559
PciI A'CATG_T
Cuts at: 818 3015 818
Size: 2197 2362
PfoI T'CCnGG_A
Cuts at: 126 126
Size: 4559
PmeI GTTT'AAAC
Cuts at: 1558 1558
Size: 4559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 3698
Size: 32 4527
PshAI GACnn'nnGTC
Cuts at: 329 329
Size: 4559
PspOMI G'GGCC_C
Cuts at: 1343 1343
Size: 4559
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1252 1284 1252
Size: 32 4527
PstI C_TGCA'G
Cuts at: 292 421 683 762 833 1470 1606 2291 292
Size: 129 262 79 71 637 136 685 2560
Fragments arranged by size:
2560 685 637 262 136 129 79 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4559
PvuII CAG'CTG
Cuts at: 744 1984 2344 744
Size: 1240 360 2959
Fragments arranged by size:
2959 1240 360
RsrII CG'GwC_CG
Cuts at: 2754 2754
Size: 4559
SacI G_AGCT'C
Cuts at: 914 1573 914
Size: 659 3900
SalI G'TCGA_C
Cuts at: 1596 1596
Size: 4559
SapI GCTCTTCn'nnn_
Cuts at: 2582 2792 2582
Size: 210 4349
SbfI CC_TGCA'GG
Cuts at: 292 683 1606 292
Size: 391 923 3245
Fragments arranged by size:
3245 923 391
ScaI AGT'ACT
Cuts at: 1144 4076 1144
Size: 2932 1627
SfcI C'TryA_G
Cuts at: 33 288 417 464 581 679 758 829 1466
Size: 255 129 47 117 98 79 71 637
Cuts at: 1466 1602 2287 3280 3471 33
Size: 136 685 993 191 1121
Fragments arranged by size:
1121 993 685 637 255 191 136 129
117 98 79 71 47
SfoI GGC'GCC
Cuts at: 1991 2239 1991
Size: 248 4311
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
SmaI CCC'GGG
Cuts at: 1581 1581
Size: 4559
SmlI C'TyrA_G
Cuts at: 501 1039 1714 2629 3121 3383 501
Size: 538 675 915 492 262 1677
Fragments arranged by size:
1677 915 675 538 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4559
SphI G_CATG'C
Cuts at: 1279 1612 2112 1279
Size: 333 500 3726
Fragments arranged by size:
3726 500 333
StyI C'CwwG_G
Cuts at: 107 695 1693 2047 107
Size: 588 998 354 2619
Fragments arranged by size:
2619 998 588 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 594 1137 2142 2275 2734 2943 594
Size: 543 1005 133 459 209 2210
Fragments arranged by size:
2210 1005 543 459 209 133
TatI w'GTAC_w
Cuts at: 1142 4074 1142
Size: 2932 1627
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 931 963 4076 4108 931
Size: 32 3113 32 1382
Fragments arranged by size:
3113 1382 32 32
Tth111I GACn'n_nGTC
Cuts at: 2356 2356
Size: 4559
XbaI T'CTAG_A
Cuts at: 1590 1590
Size: 4559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1142 2054 1142
Size: 912 3647
XmaI C'CCGG_G
Cuts at: 1579 1579
Size: 4559
XmnI GAAnn'nnTTC
Cuts at: 1360 1360
Size: 4559
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PciI PfoI PmeI PpiI
PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmtI BpmI
Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I
Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PflMI
PmlI PpuMI PsiI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI