Restriction map for
pF1KB5832
(Circular) (Six-base) MAPSORT of: pF1KB5832.seq Check: 3335 from: 1 to: 4556
pF1KB5832 4556 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 322 1081 322
Size: 759 3797
AccI GT'mk_AC
Cuts at: 1594 1594
Size: 4556
Acc65I G'GTAC_C
Cuts at: 1572 1572
Size: 4556
AclI AA'CG_TT
Cuts at: 4396 4396
Size: 4556
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 2381 2813 3560 3828 410
Size: 1971 432 747 268 1138
Fragments arranged by size:
1971 1138 747 432 268
AflIII A'CryG_T
Cuts at: 1908 3012 3989 1908
Size: 1104 977 2475
Fragments arranged by size:
2475 1104 977
AgeI A'CCGG_T
Cuts at: 2944 2944
Size: 4556
AleI CACnn'nnGTG
Cuts at: 277 598 277
Size: 321 4235
AlwNI CAG_nnn'CTG
Cuts at: 673 1861 3428 673
Size: 1188 1567 1801
Fragments arranged by size:
1801 1567 1188
ApaI G_GGCC'C
Cuts at: 133 139 569 1283 133
Size: 6 430 714 3406
Fragments arranged by size:
3406 714 430 6
ApaLI G'TGCA_C
Cuts at: 306 3326 306
Size: 3020 1536
ApoI r'AATT_y
Cuts at: 1560 3909 4165 1560
Size: 2349 256 1951
Fragments arranged by size:
2349 1951 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4556
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
AvaI C'yCGr_G
Cuts at: 132 138 684 1075 1576 132
Size: 6 546 391 501 3112
Fragments arranged by size:
3112 546 501 391 6
BamHI G'GATC_C
Cuts at: 486 1581 486
Size: 1095 3461
BanI G'GyrC_C
Cuts at: 194 825 1009 1023 1080 1272 1572 1986 2234
Size: 631 184 14 57 192 300 414 248
Cuts at: 2234 2269 194
Size: 35 2481
Fragments arranged by size:
2481 631 414 300 248 192 184 57
35 14
BanII G_rGCy'C
Cuts at: 133 139 569 610 1283 1570 2600 133
Size: 6 430 41 673 287 1030 2089
Fragments arranged by size:
2089 1030 673 430 287 41 6
BbeI G_GCGC'C
Cuts at: 1276 1990 2238 1276
Size: 714 248 3594
Fragments arranged by size:
3594 714 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1092 1126 1092
Size: 34 4522
BciVI GTATCCnnnnn_n'
Cuts at: 2454 3215 4556 2454
Size: 761 1341 2454
Fragments arranged by size:
2454 1341 761
BclI T'GATC_A
Cuts at: 2076 2076
Size: 4556
BglI GCCn_nnn'nGGC
Cuts at: 143 143
Size: 4556
BglII A'GATC_T
Cuts at: 2071 4108 2071
Size: 2037 2519
BlpI GC'TnA_GC
Cuts at: 1668 1668
Size: 4556
Bme1580I G_kGCm'C
Cuts at: 133 139 310 569 1085 1283 2181 2274 3330
Size: 6 171 259 516 198 898 93 1056
Cuts at: 3330 133
Size: 1359
Fragments arranged by size:
1359 1056 898 516 259 198 171 93
6
BmgBI CAC'GTC
Cuts at: 1056 1056
Size: 4556
BmrI ACTGGGnnnn_n'
Cuts at: 1221 1346 1768 1849 2184 4328 1221
Size: 125 422 81 335 2144 1449
Fragments arranged by size:
2144 1449 422 335 125 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 198 2923 198
Size: 2725 1831
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 926 1123 496
Size: 430 197 3929
Fragments arranged by size:
3929 430 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1732 2611 3103 3401 1732
Size: 879 492 298 2887
Fragments arranged by size:
2887 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 794 4260 794
Size: 3466 1090
BsaAI yAC'GTr
Cuts at: 279 1909 279
Size: 1630 2926
BsaBI GATnn'nnATC
Cuts at: 6 2070 4107 6
Size: 2064 2037 455
Fragments arranged by size:
2064 2037 455
BsaHI Gr'CG_yC
Cuts at: 1273 1987 2235 2937 4433 1273
Size: 714 248 702 1496 1396
Fragments arranged by size:
1496 1396 714 702 248
BsaWI w'CCGG_w
Cuts at: 1745 2266 2944 3218 3365 3776 1745
Size: 521 678 274 147 411 2525
Fragments arranged by size:
2525 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 178 208 178
Size: 30 4526
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 744 744
Size: 4556
BseYI C'CCAG_C
Cuts at: 213 260 913 968 1303 1359 1468 1981 2739
Size: 47 653 55 335 56 109 513 758
Cuts at: 2739 3316 3812 213
Size: 577 496 957
Fragments arranged by size:
957 758 653 577 513 496 335 109
56 55 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 638 797 622
Size: 16 159 4381
Fragments arranged by size:
4381 159 16
BsiEI CG_ry'CG
Cuts at: 104 2098 2144 3352 104
Size: 1994 46 1208 1308
Fragments arranged by size:
1994 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 310 736 1570 2348 2538 3330 310
Size: 426 834 778 190 792 1536
Fragments arranged by size:
1536 834 792 778 426 190
BsmI GAATG_Cn'
Cuts at: 841 841
Size: 4556
BsmBI CGTCTCn'nnnn_
Cuts at: 665 665
Size: 4556
Bsp1286I G_dGCh'C
Cuts at: 133 139 310 569 610 736 1085 1283 1570
Size: 6 171 259 41 126 349 198 287
Cuts at: 1570 2181 2274 2348 2538 2600 3330 133
Size: 611 93 74 190 62 730 1359
Fragments arranged by size:
1359 730 611 349 287 259 198 190
171 126 93 74 62 41 6
BspEI T'CCGG_A
Cuts at: 3776 3776
Size: 4556
BspHI T'CATG_A
Cuts at: 1490 3732 1490
Size: 2242 2314
BspMI ACCTGCnnnn'nnnn_
Cuts at: 322 788 1081 1606 1820 2122 2503 322
Size: 466 293 525 214 302 381 2375
Fragments arranged by size:
2375 525 466 381 302 293 214
BsrBI CCG'CTC
Cuts at: 50 1137 2848 2902 4386 4551 50
Size: 1087 1711 54 1484 165 55
Fragments arranged by size:
1711 1484 1087 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2468 2468
Size: 4556
BsrFI r'CCGG_y
Cuts at: 2554 2735 2944 2554
Size: 181 209 4166
Fragments arranged by size:
4166 209 181
BssSI C'ACGA_G
Cuts at: 2827 3185 2827
Size: 358 4198
BstAPI GCAn_nnn'nTGC
Cuts at: 314 847 1933 314
Size: 533 1086 2937
Fragments arranged by size:
2937 1086 533
BstBI TT'CG_AA
Cuts at: 2917 2917
Size: 4556
BstEII G'GTnAC_C
Cuts at: 270 270
Size: 4556
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1020 1264 1269 1468 114
Size: 906 244 5 199 3202
Fragments arranged by size:
3202 906 244 199 5
BstYI r'GATC_y
Cuts at: 1 486 1472 1581 2048 2071 2406 2652 3664
Size: 485 986 109 467 23 335 246 1012
Cuts at: 3664 3750 3762 4108 1
Size: 86 12 346 449
Fragments arranged by size:
1012 986 485 467 449 346 335 246
109 86 23 12
BtgI C'CryG_G
Cuts at: 107 290 411 107
Size: 183 121 4252
Fragments arranged by size:
4252 183 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2506 2687 2850 2506
Size: 181 163 4212
Fragments arranged by size:
4212 181 163
BtsI GCAGTG_nn'
Cuts at: 243 591 912 1305 1331 1799 243
Size: 348 321 393 26 468 3000
Fragments arranged by size:
3000 468 393 348 321 26
DraI TTT'AAA
Cuts at: 1555 1555
Size: 4556
DrdI GACnn_nn'nnGTC
Cuts at: 2262 3120 2262
Size: 858 3698
EaeI y'GGCC_r
Cuts at: 1122 2141 2315 2706 2733 4078 1122
Size: 1019 174 391 27 1345 1600
Fragments arranged by size:
1600 1345 1019 391 174 27
EagI C'GGCC_G
Cuts at: 2141 2141
Size: 4556
EarI CTCTTCn'nnn_
Cuts at: 526 924 1114 1416 2579 2789 526
Size: 398 190 302 1163 210 2293
Fragments arranged by size:
2293 1163 398 302 210 190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 181 187 2764 3074 3220 4366 181
Size: 6 2577 310 146 1146 371
Fragments arranged by size:
2577 1146 371 310 146 6
EcoICRI GAG'CTC
Cuts at: 1568 1568
Size: 4556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 496 926 1123 2381 2813 3560 3828 410
Size: 86 430 197 1258 432 747 268 1138
Fragments arranged by size:
1258 1138 747 432 430 268 197 86
EcoNI CCTnn'n_nnAGG
Cuts at: 872 872
Size: 4556
EcoO109I rG'GnC_Cy
Cuts at: 129 565 1072 1128 1279 1340 1695 129
Size: 436 507 56 151 61 355 2990
Fragments arranged by size:
2990 507 436 355 151 61 56
EcoRI G'AATT_C
Cuts at: 1560 1560
Size: 4556
EcoRV GAT'ATC
Cuts at: 285 285
Size: 4556
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 4556
FspI TGC'GCA
Cuts at: 2337 2337
Size: 4556
HaeII r_GCGC'y
Cuts at: 1276 1990 2238 3260 4239 1276
Size: 714 248 1022 979 1593
Fragments arranged by size:
1593 1022 979 714 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 527 559 1414 1446 2398 2428 2430 2460 527
Size: 32 855 32 952 30 2 30 2623
Fragments arranged by size:
2623 952 855 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1595 1595
Size: 4556
KasI G'GCGC_C
Cuts at: 1272 1986 2234 1272
Size: 714 248 3594
Fragments arranged by size:
3594 714 248
KpnI G_GTAC'C
Cuts at: 1576 1576
Size: 4556
MluI A'CGCG_T
Cuts at: 3989 3989
Size: 4556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1555 3227 3411 1555
Size: 1672 184 2700
Fragments arranged by size:
2700 1672 184
MscI TGG'CCA
Cuts at: 1124 2317 1124
Size: 1193 3363
MslI CAynn'nnrTG
Cuts at: 112 277 598 2545 2672 112
Size: 165 321 1947 127 1996
Fragments arranged by size:
1996 1947 321 165 127
MspA1I CmG'CkG
Cuts at: 155 946 1087 1236 1368 1468 1667 1981 2341
Size: 791 141 149 132 100 199 314 360
Cuts at: 2341 3354 3599 155
Size: 1013 245 1112
Fragments arranged by size:
1112 1013 791 360 314 245 199 149
141 132 100
NaeI GCC'GGC
Cuts at: 2737 2737
Size: 4556
NarI GG'CG_CC
Cuts at: 1273 1987 2235 1273
Size: 714 248 3594
Fragments arranged by size:
3594 714 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4556
NgoMIV G'CCGG_C
Cuts at: 2735 2735
Size: 4556
NspI r_CATG'y
Cuts at: 1609 2109 3016 1609
Size: 500 907 3149
Fragments arranged by size:
3149 907 500
PciI A'CATG_T
Cuts at: 3012 3012
Size: 4556
PflMI CCAn_nnn'nTGG
Cuts at: 1336 1336
Size: 4556
PfoI T'CCnGG_A
Cuts at: 512 512
Size: 4556
PmeI GTTT'AAAC
Cuts at: 1555 1555
Size: 4556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4524
PpuMI rG'GwC_Cy
Cuts at: 1072 1128 1072
Size: 56 4500
PspOMI G'GGCC_C
Cuts at: 129 135 565 1279 129
Size: 6 430 714 3406
Fragments arranged by size:
3406 714 430 6
PspXI vC'TCGA_Gb
Cuts at: 684 684
Size: 4556
PstI C_TGCA'G
Cuts at: 226 984 1092 1603 2288 226
Size: 758 108 511 685 2494
Fragments arranged by size:
2494 758 685 511 108
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4556
PvuII CAG'CTG
Cuts at: 946 1468 1981 2341 946
Size: 522 513 360 3161
Fragments arranged by size:
3161 522 513 360
RsrII CG'GwC_CG
Cuts at: 2751 2751
Size: 4556
SacI G_AGCT'C
Cuts at: 1570 1570
Size: 4556
SalI G'TCGA_C
Cuts at: 1593 1593
Size: 4556
SanDI GG'GwC_CC
Cuts at: 1072 1072
Size: 4556
SapI GCTCTTCn'nnn_
Cuts at: 924 1416 2579 2789 924
Size: 492 1163 210 2691
Fragments arranged by size:
2691 1163 492 210
SbfI CC_TGCA'GG
Cuts at: 984 1603 984
Size: 619 3937
ScaI AGT'ACT
Cuts at: 774 4073 774
Size: 3299 1257
SfcI C'TryA_G
Cuts at: 33 222 980 1088 1599 2284 3277 3468 33
Size: 189 758 108 511 685 993 191 1121
Fragments arranged by size:
1121 993 758 685 511 191 189 108
SfoI GGC'GCC
Cuts at: 1274 1988 2236 1274
Size: 714 248 3594
Fragments arranged by size:
3594 714 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
SmaI CCC'GGG
Cuts at: 134 140 1077 1578 134
Size: 6 937 501 3112
Fragments arranged by size:
3112 937 501 6
SmlI C'TyrA_G
Cuts at: 684 1711 2626 3118 3380 684
Size: 1027 915 492 262 1860
Fragments arranged by size:
1860 1027 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4556
SphI G_CATG'C
Cuts at: 1609 2109 1609
Size: 500 4056
SrfI GCCC'GGGC
Cuts at: 134 140 134
Size: 6 4550
StyI C'CwwG_G
Cuts at: 107 873 1690 2044 107
Size: 766 817 354 2619
Fragments arranged by size:
2619 817 766 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 205 634 1347 2139 2272 2731 2940 205
Size: 429 713 792 133 459 209 1821
Fragments arranged by size:
1821 792 713 459 429 209 133
TatI w'GTAC_w
Cuts at: 772 4071 772
Size: 3299 1257
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 178 210 1064 1096 1284 1316 1355 1387 4073
Size: 32 854 32 188 32 39 32 2686
Cuts at: 4073 4105 178
Size: 32 629
Fragments arranged by size:
2686 854 629 188 39 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 556 2353 556
Size: 1797 2759
XbaI T'CTAG_A
Cuts at: 1587 1587
Size: 4556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1545 2051 1545
Size: 506 4050
XhoI C'TCGA_G
Cuts at: 684 684
Size: 4556
XmaI C'CCGG_G
Cuts at: 132 138 1075 1576 132
Size: 6 937 501 3112
Fragments arranged by size:
3112 937 501 6
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsiWI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PsrI SacII SexAI
SfiI SgrAI SnaBI SspI StuI SwaI XmnI ZraI