Restriction map for
pF1KB5825
(Circular) (Six-base) MAPSORT of: pF1KB5825.seq Check: 7700 from: 1 to: 3488
pF1KB5825 3488 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 526 526
Size: 3488
Acc65I G'GTAC_C
Cuts at: 504 504
Size: 3488
AclI AA'CG_TT
Cuts at: 3328 3328
Size: 3488
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 224 1313 1745 2492 2760 173
Size: 51 1089 432 747 268 901
Fragments arranged by size:
1089 901 747 432 268 51
AflIII A'CryG_T
Cuts at: 840 1944 2921 840
Size: 1104 977 1407
Fragments arranged by size:
1407 1104 977
AgeI A'CCGG_T
Cuts at: 1876 1876
Size: 3488
AlwNI CAG_nnn'CTG
Cuts at: 793 2360 793
Size: 1567 1921
ApaLI G'TGCA_C
Cuts at: 2258 2258
Size: 3488
ApoI r'AATT_y
Cuts at: 180 492 2841 3097 180
Size: 312 2349 256 571
Fragments arranged by size:
2349 571 312 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3488
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3488
AvaI C'yCGr_G
Cuts at: 508 508
Size: 3488
BamHI G'GATC_C
Cuts at: 513 513
Size: 3488
BanI G'GyrC_C
Cuts at: 504 918 1166 1201 504
Size: 414 248 35 2791
Fragments arranged by size:
2791 414 248 35
BanII G_rGCy'C
Cuts at: 502 1532 502
Size: 1030 2458
BbeI G_GCGC'C
Cuts at: 922 1170 922
Size: 248 3240
BciVI GTATCCnnnnn_n'
Cuts at: 1386 2147 3488 1386
Size: 761 1341 1386
Fragments arranged by size:
1386 1341 761
BclI T'GATC_A
Cuts at: 330 1008 330
Size: 678 2810
BfrBI ATG'CAT
Cuts at: 325 325
Size: 3488
BglI GCCn_nnn'nGGC
Cuts at: 130 394 130
Size: 264 3224
BglII A'GATC_T
Cuts at: 1003 3040 1003
Size: 2037 1451
BlpI GC'TnA_GC
Cuts at: 600 600
Size: 3488
Bme1580I G_kGCm'C
Cuts at: 1113 1206 2262 1113
Size: 93 1056 2339
Fragments arranged by size:
2339 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 700 781 1116 3260 700
Size: 81 335 2144 928
Fragments arranged by size:
2144 928 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1855 1855
Size: 3488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 664 1543 2035 2333 430
Size: 234 879 492 298 1585
Fragments arranged by size:
1585 879 492 298 234
BsaI GGTCTCn'nnnn_
Cuts at: 3192 3192
Size: 3488
BsaAI yAC'GTr
Cuts at: 841 841
Size: 3488
BsaBI GATnn'nnATC
Cuts at: 6 1002 3039 6
Size: 996 2037 455
Fragments arranged by size:
2037 996 455
BsaHI Gr'CG_yC
Cuts at: 919 1167 1869 3365 919
Size: 248 702 1496 1042
Fragments arranged by size:
1496 1042 702 248
BsaWI w'CCGG_w
Cuts at: 677 1198 1876 2150 2297 2708 677
Size: 521 678 274 147 411 1457
Fragments arranged by size:
1457 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 418 448 418
Size: 30 3458
BseYI C'CCAG_C
Cuts at: 913 1671 2248 2744 913
Size: 758 577 496 1657
Fragments arranged by size:
1657 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1030 1076 2284 104
Size: 926 46 1208 1308
Fragments arranged by size:
1308 1208 926 46
BsiHKAI G_wGCw'C
Cuts at: 502 1280 1470 2262 502
Size: 778 190 792 1728
Fragments arranged by size:
1728 792 778 190
BsmI GAATG_Cn'
Cuts at: 327 327
Size: 3488
Bsp1286I G_dGCh'C
Cuts at: 502 1113 1206 1280 1470 1532 2262 502
Size: 611 93 74 190 62 730 1728
Fragments arranged by size:
1728 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2708 2708
Size: 3488
BspHI T'CATG_A
Cuts at: 2664 2664
Size: 3488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 538 752 1054 1435 538
Size: 214 302 381 2591
Fragments arranged by size:
2591 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1780 1834 3318 3483 50
Size: 1730 54 1484 165 55
Fragments arranged by size:
1730 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1400 1400
Size: 3488
BsrFI r'CCGG_y
Cuts at: 1486 1667 1876 1486
Size: 181 209 3098
Fragments arranged by size:
3098 209 181
BssSI C'ACGA_G
Cuts at: 1759 2117 1759
Size: 358 3130
BstAPI GCAn_nnn'nTGC
Cuts at: 865 865
Size: 3488
BstBI TT'CG_AA
Cuts at: 1849 1849
Size: 3488
BstYI r'GATC_y
Cuts at: 1 471 513 980 1003 1338 1584 2596 2682
Size: 470 42 467 23 335 246 1012 86
Cuts at: 2682 2694 3040 1
Size: 12 346 449
Fragments arranged by size:
1012 470 467 449 346 335 246 86
42 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3488
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 127 1438 1619 1782 127
Size: 1311 181 163 1833
Fragments arranged by size:
1833 1311 181 163
BtsI GCAGTG_nn'
Cuts at: 731 731
Size: 3488
DraI TTT'AAA
Cuts at: 487 487
Size: 3488
DrdI GACnn_nn'nnGTC
Cuts at: 242 1194 2052 242
Size: 952 858 1678
Fragments arranged by size:
1678 952 858
EaeI y'GGCC_r
Cuts at: 122 1073 1247 1638 1665 3010 122
Size: 951 174 391 27 1345 600
Fragments arranged by size:
1345 951 600 391 174 27
EagI C'GGCC_G
Cuts at: 1073 1073
Size: 3488
EarI CTCTTCn'nnn_
Cuts at: 1511 1721 1511
Size: 210 3278
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1696 2006 2152 3298 1696
Size: 310 146 1146 1886
Fragments arranged by size:
1886 1146 310 146
EcoICRI GAG'CTC
Cuts at: 500 500
Size: 3488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 224 1313 1745 2492 2760 173
Size: 51 1089 432 747 268 901
Fragments arranged by size:
1089 901 747 432 268 51
EcoO109I rG'GnC_Cy
Cuts at: 627 627
Size: 3488
EcoRI G'AATT_C
Cuts at: 492 492
Size: 3488
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1507 1507
Size: 3488
FspI TGC'GCA
Cuts at: 1269 1269
Size: 3488
HaeII r_GCGC'y
Cuts at: 922 1170 2192 3171 922
Size: 248 1022 979 1239
Fragments arranged by size:
1239 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 322 354 1330 1360 1362 1392 322
Size: 32 976 30 2 30 2418
Fragments arranged by size:
2418 976 32 30 30 2
HincII GTy'rAC
Cuts at: 527 527
Size: 3488
HindIII A'AGCT_T
Cuts at: 439 439
Size: 3488
KasI G'GCGC_C
Cuts at: 918 1166 918
Size: 248 3240
KpnI G_GTAC'C
Cuts at: 508 508
Size: 3488
MluI A'CGCG_T
Cuts at: 2921 2921
Size: 3488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2159 2343 2159
Size: 184 3304
MscI TGG'CCA
Cuts at: 1249 1249
Size: 3488
MslI CAynn'nnrTG
Cuts at: 112 1477 1604 112
Size: 1365 127 1996
Fragments arranged by size:
1996 1365 127
MspA1I CmG'CkG
Cuts at: 202 599 913 1273 2286 2531 202
Size: 397 314 360 1013 245 1159
Fragments arranged by size:
1159 1013 397 360 314 245
NaeI GCC'GGC
Cuts at: 1669 1669
Size: 3488
NarI GG'CG_CC
Cuts at: 919 1167 919
Size: 248 3240
NcoI C'CATG_G
Cuts at: 107 107
Size: 3488
NgoMIV G'CCGG_C
Cuts at: 1667 1667
Size: 3488
NsiI A_TGCA'T
Cuts at: 327 327
Size: 3488
NspI r_CATG'y
Cuts at: 329 541 1041 1948 329
Size: 212 500 907 1869
Fragments arranged by size:
1869 907 500 212
PciI A'CATG_T
Cuts at: 1944 1944
Size: 3488
PmeI GTTT'AAAC
Cuts at: 487 487
Size: 3488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2627 2659 2627
Size: 32 3456
PstI C_TGCA'G
Cuts at: 535 1220 535
Size: 685 2803
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3488
PvuII CAG'CTG
Cuts at: 202 913 1273 202
Size: 711 360 2417
Fragments arranged by size:
2417 711 360
RsrII CG'GwC_CG
Cuts at: 1683 1683
Size: 3488
SacI G_AGCT'C
Cuts at: 502 502
Size: 3488
SalI G'TCGA_C
Cuts at: 525 525
Size: 3488
SapI GCTCTTCn'nnn_
Cuts at: 1511 1721 1511
Size: 210 3278
SbfI CC_TGCA'GG
Cuts at: 535 535
Size: 3488
ScaI AGT'ACT
Cuts at: 3005 3005
Size: 3488
SfcI C'TryA_G
Cuts at: 33 531 1216 2209 2400 33
Size: 498 685 993 191 1121
Fragments arranged by size:
1121 993 685 498 191
SfoI GGC'GCC
Cuts at: 920 1168 920
Size: 248 3240
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3488
SmaI CCC'GGG
Cuts at: 510 510
Size: 3488
SmlI C'TyrA_G
Cuts at: 445 643 1558 2050 2312 445
Size: 198 915 492 262 1621
Fragments arranged by size:
1621 915 492 262 198
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3488
SphI G_CATG'C
Cuts at: 541 1041 541
Size: 500 2988
StyI C'CwwG_G
Cuts at: 107 622 976 107
Size: 515 354 2619
Fragments arranged by size:
2619 515 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1071 1204 1663 1872 1071
Size: 133 459 209 2687
Fragments arranged by size:
2687 459 209 133
TatI w'GTAC_w
Cuts at: 377 3003 377
Size: 2626 862
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 3456
Tth111I GACn'n_nGTC
Cuts at: 1285 1285
Size: 3488
XbaI T'CTAG_A
Cuts at: 519 519
Size: 3488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 983 983
Size: 3488
XmaI C'CCGG_G
Cuts at: 508 508
Size: 3488
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BmgBI
BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI