Restriction map for
pF1KB5823
(Circular) (Six-base) MAPSORT of: pF1KB5823.seq Check: 3192 from: 1 to: 3557
pF1KB5823 3557 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 595 595
Size: 3557
Acc65I G'GTAC_C
Cuts at: 573 573
Size: 3557
AclI AA'CG_TT
Cuts at: 3397 3397
Size: 3557
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 482 1382 1814 2561 2829 313
Size: 169 900 432 747 268 1041
Fragments arranged by size:
1041 900 747 432 268 169
AflIII A'CryG_T
Cuts at: 909 2013 2990 909
Size: 1104 977 1476
Fragments arranged by size:
1476 1104 977
AgeI A'CCGG_T
Cuts at: 1945 1945
Size: 3557
AleI CACnn'nnGTG
Cuts at: 275 275
Size: 3557
AlwNI CAG_nnn'CTG
Cuts at: 862 2429 862
Size: 1567 1990
ApaLI G'TGCA_C
Cuts at: 268 2327 268
Size: 2059 1498
ApoI r'AATT_y
Cuts at: 187 561 2910 3166 187
Size: 374 2349 256 578
Fragments arranged by size:
2349 578 374 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3557
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3557
AvaI C'yCGr_G
Cuts at: 577 577
Size: 3557
BamHI G'GATC_C
Cuts at: 456 582 456
Size: 126 3431
BanI G'GyrC_C
Cuts at: 573 987 1235 1270 573
Size: 414 248 35 2860
Fragments arranged by size:
2860 414 248 35
BanII G_rGCy'C
Cuts at: 571 1601 571
Size: 1030 2527
BbeI G_GCGC'C
Cuts at: 991 1239 991
Size: 248 3309
BciVI GTATCCnnnnn_n'
Cuts at: 1455 2216 3557 1455
Size: 761 1341 1455
Fragments arranged by size:
1455 1341 761
BclI T'GATC_A
Cuts at: 248 1077 248
Size: 829 2728
BglII A'GATC_T
Cuts at: 1072 3109 1072
Size: 2037 1520
BlpI GC'TnA_GC
Cuts at: 669 669
Size: 3557
Bme1580I G_kGCm'C
Cuts at: 152 170 272 1182 1275 2331 152
Size: 18 102 910 93 1056 1378
Fragments arranged by size:
1378 1056 910 102 93 18
BmrI ACTGGGnnnn_n'
Cuts at: 769 850 1185 3329 769
Size: 81 335 2144 997
Fragments arranged by size:
2144 997 335 81
BmtI G_CTAG'C
Cuts at: 122 122
Size: 3557
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1924 1924
Size: 3557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 199
Size: 3557
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 1612 2104 2402 733
Size: 879 492 298 1888
Fragments arranged by size:
1888 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3261 3261
Size: 3557
BsaAI yAC'GTr
Cuts at: 144 910 144
Size: 766 2791
BsaBI GATnn'nnATC
Cuts at: 6 443 1071 3108 6
Size: 437 628 2037 455
Fragments arranged by size:
2037 628 455 437
BsaHI Gr'CG_yC
Cuts at: 988 1236 1938 3434 988
Size: 248 702 1496 1111
Fragments arranged by size:
1496 1111 702 248
BsaWI w'CCGG_w
Cuts at: 746 1267 1945 2219 2366 2777 746
Size: 521 678 274 147 411 1526
Fragments arranged by size:
1526 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 982 1740 2317 2813 982
Size: 758 577 496 1726
Fragments arranged by size:
1726 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 196 1099 1145 2353 104
Size: 92 903 46 1208 1308
Fragments arranged by size:
1308 1208 903 92 46
BsiHKAI G_wGCw'C
Cuts at: 272 401 571 1349 1539 2331 272
Size: 129 170 778 190 792 1498
Fragments arranged by size:
1498 792 778 190 170 129
Bsp1286I G_dGCh'C
Cuts at: 152 170 272 401 571 1182 1275 1349 1539
Size: 18 102 129 170 611 93 74 190
Cuts at: 1539 1601 2331 152
Size: 62 730 1378
Fragments arranged by size:
1378 730 611 190 170 129 102 93
74 62 18
BspEI T'CCGG_A
Cuts at: 2777 2777
Size: 3557
BspHI T'CATG_A
Cuts at: 245 2733 245
Size: 2488 1069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 140 607 821 1123 1504 140
Size: 467 214 302 381 2193
Fragments arranged by size:
2193 467 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1849 1903 3387 3552 50
Size: 1799 54 1484 165 55
Fragments arranged by size:
1799 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1469 1469
Size: 3557
BsrFI r'CCGG_y
Cuts at: 1555 1736 1945 1555
Size: 181 209 3167
Fragments arranged by size:
3167 209 181
BssSI C'ACGA_G
Cuts at: 169 1828 2186 169
Size: 1659 358 1540
Fragments arranged by size:
1659 1540 358
BstAPI GCAn_nnn'nTGC
Cuts at: 934 934
Size: 3557
BstBI TT'CG_AA
Cuts at: 185 1918 185
Size: 1733 1824
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3557
BstYI r'GATC_y
Cuts at: 1 456 582 1049 1072 1407 1653 2665 2751
Size: 455 126 467 23 335 246 1012 86
Cuts at: 2751 2763 3109 1
Size: 12 346 449
Fragments arranged by size:
1012 467 455 449 346 335 246 126
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3557
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1507 1688 1851 1507
Size: 181 163 3213
Fragments arranged by size:
3213 181 163
BtsI GCAGTG_nn'
Cuts at: 800 800
Size: 3557
DraI TTT'AAA
Cuts at: 556 556
Size: 3557
DrdI GACnn_nn'nnGTC
Cuts at: 1263 2121 1263
Size: 858 2699
EaeI y'GGCC_r
Cuts at: 193 363 1142 1316 1707 1734 3079 193
Size: 170 779 174 391 27 1345 671
Fragments arranged by size:
1345 779 671 391 174 170 27
EagI C'GGCC_G
Cuts at: 193 1142 193
Size: 949 2608
EarI CTCTTCn'nnn_
Cuts at: 289 1580 1790 289
Size: 1291 210 2056
Fragments arranged by size:
2056 1291 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1765 2075 2221 3367 1765
Size: 310 146 1146 1955
Fragments arranged by size:
1955 1146 310 146
EcoICRI GAG'CTC
Cuts at: 569 569
Size: 3557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 313 482 1382 1814 2561 2829 199
Size: 114 169 900 432 747 268 927
Fragments arranged by size:
927 900 747 432 268 169 114
EcoO109I rG'GnC_Cy
Cuts at: 696 696
Size: 3557
EcoRI G'AATT_C
Cuts at: 561 561
Size: 3557
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1576 1576
Size: 3557
FspI TGC'GCA
Cuts at: 1338 1338
Size: 3557
HaeII r_GCGC'y
Cuts at: 991 1239 2261 3240 991
Size: 248 1022 979 1308
Fragments arranged by size:
1308 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 388 420 1399 1429 1431 1461 388
Size: 32 979 30 2 30 2484
Fragments arranged by size:
2484 979 32 30 30 2
HincII GTy'rAC
Cuts at: 596 596
Size: 3557
HindIII A'AGCT_T
Cuts at: 205 205
Size: 3557
KasI G'GCGC_C
Cuts at: 987 1235 987
Size: 248 3309
KpnI G_GTAC'C
Cuts at: 577 577
Size: 3557
MfeI C'AATT_G
Cuts at: 548 548
Size: 3557
MluI A'CGCG_T
Cuts at: 2990 2990
Size: 3557
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2228 2412 2228
Size: 184 3373
MscI TGG'CCA
Cuts at: 1318 1318
Size: 3557
MslI CAynn'nnrTG
Cuts at: 112 275 1546 1673 112
Size: 163 1271 127 1996
Fragments arranged by size:
1996 1271 163 127
MspA1I CmG'CkG
Cuts at: 668 982 1342 2355 2600 668
Size: 314 360 1013 245 1625
Fragments arranged by size:
1625 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1738 1738
Size: 3557
NarI GG'CG_CC
Cuts at: 988 1236 988
Size: 248 3309
NcoI C'CATG_G
Cuts at: 107 107
Size: 3557
NgoMIV G'CCGG_C
Cuts at: 1736 1736
Size: 3557
NheI G'CTAG_C
Cuts at: 118 118
Size: 3557
NspI r_CATG'y
Cuts at: 610 1110 2017 610
Size: 500 907 2150
Fragments arranged by size:
2150 907 500
PciI A'CATG_T
Cuts at: 2013 2013
Size: 3557
PmeI GTTT'AAAC
Cuts at: 556 556
Size: 3557
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2696 2728 2696
Size: 32 3525
PstI C_TGCA'G
Cuts at: 604 1289 604
Size: 685 2872
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3557
PvuII CAG'CTG
Cuts at: 982 1342 982
Size: 360 3197
RsrII CG'GwC_CG
Cuts at: 1752 1752
Size: 3557
SacI G_AGCT'C
Cuts at: 571 571
Size: 3557
SalI G'TCGA_C
Cuts at: 594 594
Size: 3557
SapI GCTCTTCn'nnn_
Cuts at: 1580 1790 1580
Size: 210 3347
SbfI CC_TGCA'GG
Cuts at: 604 604
Size: 3557
ScaI AGT'ACT
Cuts at: 3074 3074
Size: 3557
SfcI C'TryA_G
Cuts at: 33 600 1285 2278 2469 33
Size: 567 685 993 191 1121
Fragments arranged by size:
1121 993 685 567 191
SfoI GGC'GCC
Cuts at: 989 1237 989
Size: 248 3309
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3557
SmaI CCC'GGG
Cuts at: 579 579
Size: 3557
SmlI C'TyrA_G
Cuts at: 712 1627 2119 2381 712
Size: 915 492 262 1888
Fragments arranged by size:
1888 915 492 262
SnaBI TAC'GTA
Cuts at: 144 144
Size: 3557
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3557
SphI G_CATG'C
Cuts at: 610 1110 610
Size: 500 3057
StuI AGG'CCT
Cuts at: 467 467
Size: 3557
StyI C'CwwG_G
Cuts at: 107 691 1045 107
Size: 584 354 2619
Fragments arranged by size:
2619 584 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1140 1273 1732 1941 1140
Size: 133 459 209 2756
Fragments arranged by size:
2756 459 209 133
TatI w'GTAC_w
Cuts at: 3072 3072
Size: 3557
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3074 3106 3074
Size: 32 3525
Tth111I GACn'n_nGTC
Cuts at: 1354 1354
Size: 3557
XbaI T'CTAG_A
Cuts at: 588 588
Size: 3557
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1052 1052
Size: 3557
XmaI C'CCGG_G
Cuts at: 577 577
Size: 3557
XmnI GAAnn'nnTTC
Cuts at: 467 467
Size: 3557
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI NdeI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SspI SwaI XhoI ZraI