Restriction map for
pF1KB5822
(Circular) (Six-base) MAPSORT of: pF1KB5822.seq Check: 5440 from: 1 to: 4742
pF1KB5822 4742 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1329 1329
Size: 4742
AccI GT'mk_AC
Cuts at: 1780 1780
Size: 4742
Acc65I G'GTAC_C
Cuts at: 1758 1758
Size: 4742
AclI AA'CG_TT
Cuts at: 4582 4582
Size: 4742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1479 1695 2567 2999 3746 4014 1479
Size: 216 872 432 747 268 2207
Fragments arranged by size:
2207 872 747 432 268 216
AflIII A'CryG_T
Cuts at: 248 693 2094 3198 4175 248
Size: 445 1401 1104 977 815
Fragments arranged by size:
1401 1104 977 815 445
AgeI A'CCGG_T
Cuts at: 3130 3130
Size: 4742
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 343 343
Size: 4742
AlwNI CAG_nnn'CTG
Cuts at: 231 941 982 1263 1356 2047 3614 231
Size: 710 41 281 93 691 1567 1359
Fragments arranged by size:
1567 1359 710 691 281 93 41
ApaI G_GGCC'C
Cuts at: 996 1375 996
Size: 379 4363
ApaLI G'TGCA_C
Cuts at: 1282 3512 1282
Size: 2230 2512
ApoI r'AATT_y
Cuts at: 1746 4095 4351 1746
Size: 2349 256 2137
Fragments arranged by size:
2349 2137 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4742
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
AvaI C'yCGr_G
Cuts at: 1277 1539 1675 1762 1277
Size: 262 136 87 4257
Fragments arranged by size:
4257 262 136 87
BamHI G'GATC_C
Cuts at: 1767 1767
Size: 4742
BanI G'GyrC_C
Cuts at: 1132 1560 1758 2172 2420 2455 1132
Size: 428 198 414 248 35 3419
Fragments arranged by size:
3419 428 414 248 198 35
BanII G_rGCy'C
Cuts at: 170 649 974 996 1375 1756 2786 170
Size: 479 325 22 379 381 1030 2126
Fragments arranged by size:
2126 1030 479 381 379 325 22
BbeI G_GCGC'C
Cuts at: 2176 2424 2176
Size: 248 4494
BbsI GAAGACnn'nnnn_
Cuts at: 168 168
Size: 4742
BbvCI CC'TCA_GC
Cuts at: 1400 1400
Size: 4742
BciVI GTATCCnnnnn_n'
Cuts at: 1264 2640 3401 4742 1264
Size: 1376 761 1341 1264
Fragments arranged by size:
1376 1341 1264 761
BclI T'GATC_A
Cuts at: 2262 2262
Size: 4742
BfrBI ATG'CAT
Cuts at: 402 402
Size: 4742
BglI GCCn_nnn'nGGC
Cuts at: 310 1687 310
Size: 1377 3365
BglII A'GATC_T
Cuts at: 2257 4294 2257
Size: 2037 2705
BlpI GC'TnA_GC
Cuts at: 1325 1854 1325
Size: 529 4213
Bme1580I G_kGCm'C
Cuts at: 996 1135 1286 1375 2367 2460 3516 996
Size: 139 151 89 992 93 1056 2222
Fragments arranged by size:
2222 1056 992 151 139 93 89
BmgBI CAC'GTC
Cuts at: 1116 1116
Size: 4742
BmrI ACTGGGnnnn_n'
Cuts at: 196 1954 2035 2370 4514 196
Size: 1758 81 335 2144 424
Fragments arranged by size:
2144 1758 424 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1227 3109 1227
Size: 1882 2860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1222 1243 1285 556
Size: 666 21 42 4013
Fragments arranged by size:
4013 666 42 21
Bpu10I CC'TnA_GC
Cuts at: 1400 1415 1507 1400
Size: 15 92 4635
Fragments arranged by size:
4635 92 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1126 1918 2797 3289 3587 1126
Size: 792 879 492 298 2281
Fragments arranged by size:
2281 879 792 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 595 4446 595
Size: 3851 891
BsaAI yAC'GTr
Cuts at: 251 696 2095 251
Size: 445 1399 2898
Fragments arranged by size:
2898 1399 445
BsaBI GATnn'nnATC
Cuts at: 6 2256 4293 6
Size: 2250 2037 455
Fragments arranged by size:
2250 2037 455
BsaHI Gr'CG_yC
Cuts at: 313 926 2173 2421 3123 4619 313
Size: 613 1247 248 702 1496 436
Fragments arranged by size:
1496 1247 702 613 436 248
BsaWI w'CCGG_w
Cuts at: 1163 1931 2452 3130 3404 3551 3962 1163
Size: 768 521 678 274 147 411 1943
Fragments arranged by size:
1943 768 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 235 265 580 610 742 772 235
Size: 30 315 30 132 30 4205
Fragments arranged by size:
4205 315 132 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 171 256 379 696 1735 171
Size: 85 123 317 1039 3178
Fragments arranged by size:
3178 1039 317 123 85
BseYI C'CCAG_C
Cuts at: 636 1334 1392 1463 1585 2167 2925 3502 3998
Size: 698 58 71 122 582 758 577 496
Cuts at: 3998 636
Size: 1380
Fragments arranged by size:
1380 758 698 582 577 496 122 71
58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 733 544
Size: 189 4553
BsiEI CG_ry'CG
Cuts at: 104 2284 2330 3538 104
Size: 2180 46 1208 1308
Fragments arranged by size:
2180 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 726 974 1286 1756 2534 2724 3516 726
Size: 248 312 470 778 190 792 1952
Fragments arranged by size:
1952 792 778 470 312 248 190
BsmI GAATG_Cn'
Cuts at: 1091 1091
Size: 4742
BsmBI CGTCTCn'nnnn_
Cuts at: 1038 1038
Size: 4742
Bsp1286I G_dGCh'C
Cuts at: 170 649 726 974 996 1135 1286 1375 1756
Size: 479 77 248 22 139 151 89 381
Cuts at: 1756 2367 2460 2534 2724 2786 3516 170
Size: 611 93 74 190 62 730 1396
Fragments arranged by size:
1396 730 611 479 381 248 190 151
139 93 89 77 74 62 22
BspEI T'CCGG_A
Cuts at: 1163 3962 1163
Size: 2799 1943
BspHI T'CATG_A
Cuts at: 3918 3918
Size: 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 272 1190 1329 1792 2006 2308 2689 272
Size: 918 139 463 214 302 381 2325
Fragments arranged by size:
2325 918 463 381 302 214 139
BsrBI CCG'CTC
Cuts at: 50 1008 3034 3088 4572 4737 50
Size: 958 2026 54 1484 165 55
Fragments arranged by size:
2026 1484 958 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2654 2654
Size: 4742
BsrFI r'CCGG_y
Cuts at: 297 2740 2921 3130 297
Size: 2443 181 209 1909
Fragments arranged by size:
2443 1909 209 181
BsrGI T'GTAC_A
Cuts at: 887 887
Size: 4742
BssSI C'ACGA_G
Cuts at: 3013 3371 3013
Size: 358 4384
BstAPI GCAn_nnn'nTGC
Cuts at: 342 408 941 1356 2119 342
Size: 66 533 415 763 2965
Fragments arranged by size:
2965 763 533 415 66
BstBI TT'CG_AA
Cuts at: 3103 3103
Size: 4742
BstXI CCAn_nnnn'nTGG
Cuts at: 1110 1200 1110
Size: 90 4652
BstYI r'GATC_y
Cuts at: 1 1602 1767 2234 2257 2592 2838 3850 3936
Size: 1601 165 467 23 335 246 1012 86
Cuts at: 3936 3948 4294 1
Size: 12 346 449
Fragments arranged by size:
1601 1012 467 449 346 335 246 165
86 23 12
BtgI C'CryG_G
Cuts at: 308 737 308
Size: 429 4313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1742 2692 2873 3036 1742
Size: 950 181 163 3448
Fragments arranged by size:
3448 950 181 163
BtsI GCAGTG_nn'
Cuts at: 1420 1630 1985 1420
Size: 210 355 4177
Fragments arranged by size:
4177 355 210
DraI TTT'AAA
Cuts at: 1741 1741
Size: 4742
DrdI GACnn_nn'nnGTC
Cuts at: 904 2448 3306 904
Size: 1544 858 2340
Fragments arranged by size:
2340 1544 858
EaeI y'GGCC_r
Cuts at: 461 740 998 2327 2501 2892 2919 4264 461
Size: 279 258 1329 174 391 27 1345 939
Fragments arranged by size:
1345 1329 939 391 279 258 174 27
EagI C'GGCC_G
Cuts at: 2327 2327
Size: 4742
EarI CTCTTCn'nnn_
Cuts at: 158 1361 2765 2975 158
Size: 1203 1404 210 1925
Fragments arranged by size:
1925 1404 1203 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2950 3260 3406 4552 2950
Size: 310 146 1146 3140
Fragments arranged by size:
3140 1146 310 146
EcoICRI GAG'CTC
Cuts at: 972 1754 972
Size: 782 3960
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 1222 1243 1285 1479 1695 2567 2999 3746
Size: 666 21 42 194 216 872 432 747
Cuts at: 3746 4014 556
Size: 268 1284
Fragments arranged by size:
1284 872 747 666 432 268 216 194
42 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1000 1000
Size: 4742
EcoO109I rG'GnC_Cy
Cuts at: 992 993 1179 1371 1383 1404 1534 1881 992
Size: 1 186 192 12 21 130 347 3853
Fragments arranged by size:
3853 347 192 186 130 21 12 1
EcoRI G'AATT_C
Cuts at: 1746 1746
Size: 4742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4742
FspI TGC'GCA
Cuts at: 331 2523 331
Size: 2192 2550
HaeII r_GCGC'y
Cuts at: 298 2176 2424 3446 4425 298
Size: 1878 248 1022 979 615
Fragments arranged by size:
1878 1022 979 615 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 580 612 1260 1292 2584 2614 2616 2646 580
Size: 32 648 32 1292 30 2 30 2676
Fragments arranged by size:
2676 1292 648 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1781 1781
Size: 4742
KasI G'GCGC_C
Cuts at: 2172 2420 2172
Size: 248 4494
KpnI G_GTAC'C
Cuts at: 1762 1762
Size: 4742
MluI A'CGCG_T
Cuts at: 4175 4175
Size: 4742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 758 785 3413 3597 758
Size: 27 2628 184 1903
Fragments arranged by size:
2628 1903 184 27
MscI TGG'CCA
Cuts at: 742 1000 2503 742
Size: 258 1503 2981
Fragments arranged by size:
2981 1503 258
MslI CAynn'nnrTG
Cuts at: 742 881 1318 2731 2858 742
Size: 139 437 1413 127 2626
Fragments arranged by size:
2626 1413 437 139 127
MspA1I CmG'CkG
Cuts at: 117 372 712 1449 1641 1853 2167 2527 3540
Size: 255 340 737 192 212 314 360 1013
Cuts at: 3540 3785 117
Size: 245 1074
Fragments arranged by size:
1074 1013 737 360 340 314 255 245
212 192
NaeI GCC'GGC
Cuts at: 299 2923 299
Size: 2624 2118
NarI GG'CG_CC
Cuts at: 2173 2421 2173
Size: 248 4494
NcoI C'CATG_G
Cuts at: 308 737 308
Size: 429 4313
NgoMIV G'CCGG_C
Cuts at: 297 2921 297
Size: 2624 2118
NsiI A_TGCA'T
Cuts at: 404 404
Size: 4742
NspI r_CATG'y
Cuts at: 336 384 411 1795 2295 3202 336
Size: 48 27 1384 500 907 1876
Fragments arranged by size:
1876 1384 907 500 48 27
PasI CC'CwG_GG
Cuts at: 642 676 1375 642
Size: 34 699 4009
Fragments arranged by size:
4009 699 34
PciI A'CATG_T
Cuts at: 3198 3198
Size: 4742
PflMI CCAn_nnn'nTGG
Cuts at: 1263 1341 1488 1263
Size: 78 147 4517
Fragments arranged by size:
4517 147 78
PfoI T'CCnGG_A
Cuts at: 1256 1619 1256
Size: 363 4379
PmeI GTTT'AAAC
Cuts at: 1741 1741
Size: 4742
PmlI CAC'GTG
Cuts at: 251 696 251
Size: 445 4297
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 4710
PpuMI rG'GwC_Cy
Cuts at: 1179 1404 1534 1179
Size: 225 130 4387
Fragments arranged by size:
4387 225 130
PshAI GACnn'nnGTC
Cuts at: 1532 1532
Size: 4742
PspOMI G'GGCC_C
Cuts at: 992 1371 992
Size: 379 4363
PspXI vC'TCGA_Gb
Cuts at: 1277 1277
Size: 4742
PstI C_TGCA'G
Cuts at: 563 1789 2474 563
Size: 1226 685 2831
Fragments arranged by size:
2831 1226 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4742
PvuII CAG'CTG
Cuts at: 117 2167 2527 117
Size: 2050 360 2332
Fragments arranged by size:
2332 2050 360
RsrII CG'GwC_CG
Cuts at: 2937 2937
Size: 4742
SacI G_AGCT'C
Cuts at: 974 1756 974
Size: 782 3960
SalI G'TCGA_C
Cuts at: 1779 1779
Size: 4742
SanDI GG'GwC_CC
Cuts at: 1534 1534
Size: 4742
SapI GCTCTTCn'nnn_
Cuts at: 2765 2975 2765
Size: 210 4532
SbfI CC_TGCA'GG
Cuts at: 1789 1789
Size: 4742
ScaI AGT'ACT
Cuts at: 4259 4259
Size: 4742
SfcI C'TryA_G
Cuts at: 33 559 1785 2470 3463 3654 33
Size: 526 1226 685 993 191 1121
Fragments arranged by size:
1226 1121 993 685 526 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1687 1687
Size: 4742
SfoI GGC'GCC
Cuts at: 2174 2422 2174
Size: 248 4494
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
SmaI CCC'GGG
Cuts at: 1541 1764 1541
Size: 223 4519
SmlI C'TyrA_G
Cuts at: 1141 1277 1897 2812 3304 3566 1141
Size: 136 620 915 492 262 2317
Fragments arranged by size:
2317 915 620 492 262 136
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4742
SphI G_CATG'C
Cuts at: 336 384 1795 2295 336
Size: 48 1411 500 2783
Fragments arranged by size:
2783 1411 500 48
StuI AGG'CCT
Cuts at: 1147 1681 1147
Size: 534 4208
StyI C'CwwG_G
Cuts at: 140 308 737 819 1001 1097 1685 1876 2230
Size: 168 429 82 182 96 588 191 354
Cuts at: 2230 140
Size: 2652
Fragments arranged by size:
2652 588 429 354 191 182 168 96
82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1477 2325 2458 2917 3126 1477
Size: 848 133 459 209 3093
Fragments arranged by size:
3093 848 459 209 133
TatI w'GTAC_w
Cuts at: 887 4257 887
Size: 3370 1372
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 235 267 685 717 742 774 4259 4291 235
Size: 32 418 32 25 32 3485 32 686
Fragments arranged by size:
3485 686 418 32 32 32 32 25
Tth111I GACn'n_nGTC
Cuts at: 814 2539 814
Size: 1725 3017
XbaI T'CTAG_A
Cuts at: 1773 1773
Size: 4742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 735 1602 2237 735
Size: 867 635 3240
Fragments arranged by size:
3240 867 635
XhoI C'TCGA_G
Cuts at: 1277 1277
Size: 4742
XmaI C'CCGG_G
Cuts at: 1539 1762 1539
Size: 223 4519
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BmtI BsiWI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PsiI PsrI
SacII SexAI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI