Restriction map for
pF1KB5819
(Circular) (Six-base) MAPSORT of: pF1KB5819.seq Check: 3560 from: 1 to: 4349
pF1KB5819 4349 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 891 1387 891
Size: 496 3853
Acc65I G'GTAC_C
Cuts at: 1365 1365
Size: 4349
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 1114 2174 2606 3353 3621 725
Size: 389 1060 432 747 268 1453
Fragments arranged by size:
1453 1060 747 432 389 268
AfeI AGC'GCT
Cuts at: 1021 1021
Size: 4349
AflIII A'CryG_T
Cuts at: 515 557 1701 2805 3782 515
Size: 42 1144 1104 977 1082
Fragments arranged by size:
1144 1104 1082 977 42
AgeI A'CCGG_T
Cuts at: 2737 2737
Size: 4349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 524 1354 524
Size: 830 3519
AlwNI CAG_nnn'CTG
Cuts at: 927 1299 1654 3221 927
Size: 372 355 1567 2055
Fragments arranged by size:
2055 1567 372 355
ApaI G_GGCC'C
Cuts at: 582 582
Size: 4349
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 429 435 806 1353 3702 3958 429
Size: 6 371 547 2349 256 820
Fragments arranged by size:
2349 820 547 371 256 6
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 591 976 1369 591
Size: 385 393 3571
Fragments arranged by size:
3571 393 385
BamHI G'GATC_C
Cuts at: 1374 1374
Size: 4349
BanI G'GyrC_C
Cuts at: 1365 1779 2027 2062 1365
Size: 414 248 35 3652
Fragments arranged by size:
3652 414 248 35
BanII G_rGCy'C
Cuts at: 582 663 885 1203 1363 2393 582
Size: 81 222 318 160 1030 2538
Fragments arranged by size:
2538 1030 318 222 160 81
BbeI G_GCGC'C
Cuts at: 1783 2031 1783
Size: 248 4101
BbvCI CC'TCA_GC
Cuts at: 761 761
Size: 4349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1057 1091 1057
Size: 34 4315
BciVI GTATCCnnnnn_n'
Cuts at: 2247 3008 4349 2247
Size: 761 1341 2247
Fragments arranged by size:
2247 1341 761
BclI T'GATC_A
Cuts at: 1869 1869
Size: 4349
BglI GCCn_nnn'nGGC
Cuts at: 187 187
Size: 4349
BglII A'GATC_T
Cuts at: 1864 3901 1864
Size: 2037 2312
BlpI GC'TnA_GC
Cuts at: 1461 1461
Size: 4349
Bme1580I G_kGCm'C
Cuts at: 582 1059 1974 2067 3123 582
Size: 477 915 93 1056 1808
Fragments arranged by size:
1808 1056 915 477 93
BmrI ACTGGGnnnn_n'
Cuts at: 156 860 1561 1642 1977 4121 156
Size: 704 701 81 335 2144 384
Fragments arranged by size:
2144 704 701 384 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 768 2716 768
Size: 1948 2401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 249 738 773 1195 233
Size: 16 489 35 422 3387
Fragments arranged by size:
3387 489 422 35 16
Bpu10I CC'TnA_GC
Cuts at: 583 761 1236 583
Size: 178 475 3696
Fragments arranged by size:
3696 475 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1147 1525 2404 2896 3194 1147
Size: 378 879 492 298 2302
Fragments arranged by size:
2302 879 492 378 298
BsaI GGTCTCn'nnnn_
Cuts at: 209 964 4053 209
Size: 755 3089 505
Fragments arranged by size:
3089 755 505
BsaAI yAC'GTr
Cuts at: 516 1702 516
Size: 1186 3163
BsaBI GATnn'nnATC
Cuts at: 6 1863 3900 6
Size: 1857 2037 455
Fragments arranged by size:
2037 1857 455
BsaHI Gr'CG_yC
Cuts at: 1780 2028 2730 4226 1780
Size: 248 702 1496 1903
Fragments arranged by size:
1903 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1538 2059 2737 3011 3158 3569 1538
Size: 521 678 274 147 411 2318
Fragments arranged by size:
2318 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 335 352 365 382 335
Size: 17 13 17 4302
Fragments arranged by size:
4302 17 17 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 994 1182 310
Size: 684 188 3477
Fragments arranged by size:
3477 684 188
BseYI C'CCAG_C
Cuts at: 163 1774 2532 3109 3605 163
Size: 1611 758 577 496 907
Fragments arranged by size:
1611 907 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 1324
Size: 4349
BsiEI CG_ry'CG
Cuts at: 104 1891 1937 3145 104
Size: 1787 46 1208 1308
Fragments arranged by size:
1787 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 416 1203 1363 2141 2331 3123 416
Size: 787 160 778 190 792 1642
Fragments arranged by size:
1642 792 787 778 190 160
BsmBI CGTCTCn'nnnn_
Cuts at: 292 292
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 416 582 663 885 1059 1203 1363 1974 2067
Size: 166 81 222 174 144 160 611 93
Cuts at: 2067 2141 2331 2393 3123 416
Size: 74 190 62 730 1642
Fragments arranged by size:
1642 730 611 222 190 174 166 160
144 93 81 74 62
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 3525 3525
Size: 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1399 1613 1915 2296 1399
Size: 214 302 381 3452
Fragments arranged by size:
3452 381 302 214
BsrBI CCG'CTC
Cuts at: 50 984 2641 2695 4179 4344 50
Size: 934 1657 54 1484 165 55
Fragments arranged by size:
1657 1484 934 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2261 2261
Size: 4349
BsrFI r'CCGG_y
Cuts at: 956 1061 2347 2528 2737 956
Size: 105 1286 181 209 2568
Fragments arranged by size:
2568 1286 209 181 105
BssSI C'ACGA_G
Cuts at: 415 2620 2978 415
Size: 2205 358 1786
Fragments arranged by size:
2205 1786 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1032 1726 1032
Size: 694 3655
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstYI r'GATC_y
Cuts at: 1 1374 1841 1864 2199 2445 3457 3543 3555
Size: 1373 467 23 335 246 1012 86 12
Cuts at: 3555 3901 1
Size: 346 449
Fragments arranged by size:
1373 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 1226 1226
Size: 4349
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 205 2299 2480 2643 205
Size: 2094 181 163 1911
Fragments arranged by size:
2094 1911 181 163
BtsI GCAGTG_nn'
Cuts at: 279 527 542 1592 279
Size: 248 15 1050 3036
Fragments arranged by size:
3036 1050 248 15
DraI TTT'AAA
Cuts at: 1348 1348
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 2055 2913 2055
Size: 858 3491
EaeI y'GGCC_r
Cuts at: 573 944 1209 1934 2108 2499 2526 3871 573
Size: 371 265 725 174 391 27 1345 1051
Fragments arranged by size:
1345 1051 725 391 371 265 174 27
EagI C'GGCC_G
Cuts at: 1934 1934
Size: 4349
EarI CTCTTCn'nnn_
Cuts at: 167 287 324 946 1313 2372 2582 167
Size: 120 37 622 367 1059 210 1934
Fragments arranged by size:
1934 1059 622 367 210 120 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2557 2867 3013 4159 2557
Size: 310 146 1146 2747
Fragments arranged by size:
2747 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1201 1361 1201
Size: 160 4189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 249 725 738 773 1114 1195 2174 2606
Size: 16 476 13 35 341 81 979 432
Cuts at: 2606 3353 3621 233
Size: 747 268 961
Fragments arranged by size:
979 961 747 476 432 341 268 81
35 16 13
EcoO109I rG'GnC_Cy
Cuts at: 579 748 1215 1488 579
Size: 169 467 273 3440
Fragments arranged by size:
3440 467 273 169
EcoRI G'AATT_C
Cuts at: 435 806 1353 435
Size: 371 547 3431
Fragments arranged by size:
3431 547 371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1329 2368 1329
Size: 1039 3310
FspI TGC'GCA
Cuts at: 2130 2130
Size: 4349
HaeII r_GCGC'y
Cuts at: 548 1023 1783 2031 3053 4032 548
Size: 475 760 248 1022 979 865
Fragments arranged by size:
1022 979 865 760 475 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 224 247 256 279 301 333 335 367 622
Size: 23 9 23 22 32 2 32 255
Cuts at: 622 654 2191 2221 2223 2253 224
Size: 32 1537 30 2 30 2320
Fragments arranged by size:
2320 1537 255 32 32 32 30 30
23 23 22 9 2 2
HincII GTy'rAC
Cuts at: 892 1388 892
Size: 496 3853
KasI G'GCGC_C
Cuts at: 1779 2027 1779
Size: 248 4101
KpnI G_GTAC'C
Cuts at: 1369 1369
Size: 4349
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 97 267 1265 3020 3204 97
Size: 170 998 1755 184 1242
Fragments arranged by size:
1755 1242 998 184 170
MscI TGG'CCA
Cuts at: 1211 2110 1211
Size: 899 3450
MslI CAynn'nnrTG
Cuts at: 2338 2465 2338
Size: 127 4222
MspA1I CmG'CkG
Cuts at: 924 1053 1071 1243 1263 1460 1774 2134 3147
Size: 129 18 172 20 197 314 360 1013
Cuts at: 3147 3392 924
Size: 245 1881
Fragments arranged by size:
1881 1013 360 314 245 197 172 129
20 18
NaeI GCC'GGC
Cuts at: 958 1063 2530 958
Size: 105 1467 2777
Fragments arranged by size:
2777 1467 105
NarI GG'CG_CC
Cuts at: 1780 2028 1780
Size: 248 4101
NcoI C'CATG_G
Cuts at: 1226 1226
Size: 4349
NgoMIV G'CCGG_C
Cuts at: 956 1061 2528 956
Size: 105 1467 2777
Fragments arranged by size:
2777 1467 105
NspI r_CATG'y
Cuts at: 561 1035 1402 1902 2809 561
Size: 474 367 500 907 2101
Fragments arranged by size:
2101 907 500 474 367
PciI A'CATG_T
Cuts at: 557 2805 557
Size: 2248 2101
PflMI CCAn_nnn'nTGG
Cuts at: 1299 1299
Size: 4349
PfoI T'CCnGG_A
Cuts at: 210 210
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PmlI CAC'GTG
Cuts at: 516 516
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1318 1350 3488 3520 1318
Size: 32 2138 32 2147
Fragments arranged by size:
2147 2138 32 32
PpuMI rG'GwC_Cy
Cuts at: 748 1215 748
Size: 467 3882
PspOMI G'GGCC_C
Cuts at: 578 578
Size: 4349
PstI C_TGCA'G
Cuts at: 534 923 1070 1396 2081 534
Size: 389 147 326 685 2802
Fragments arranged by size:
2802 685 389 326 147
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 1243 1263 1774 2134 1243
Size: 20 511 360 3458
Fragments arranged by size:
3458 511 360 20
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 1203 1363 1203
Size: 160 4189
SalI G'TCGA_C
Cuts at: 890 1386 890
Size: 496 3853
SanDI GG'GwC_CC
Cuts at: 748 748
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 167 946 1313 2372 2582 167
Size: 779 367 1059 210 1934
Fragments arranged by size:
1934 1059 779 367 210
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 710 3866 710
Size: 3156 1193
SfcI C'TryA_G
Cuts at: 33 530 919 1066 1392 2077 3070 3261 33
Size: 497 389 147 326 685 993 191 1121
Fragments arranged by size:
1121 993 685 497 389 326 191 147
SfoI GGC'GCC
Cuts at: 1781 2029 1781
Size: 248 4101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 593 1371 593
Size: 778 3571
SmlI C'TyrA_G
Cuts at: 1162 1504 2419 2911 3173 1162
Size: 342 915 492 262 2338
Fragments arranged by size:
2338 915 492 342 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1035 1402 1902 1035
Size: 367 500 3482
Fragments arranged by size:
3482 500 367
StyI C'CwwG_G
Cuts at: 219 1226 1483 1837 219
Size: 1007 257 354 2731
Fragments arranged by size:
2731 1007 354 257
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1932 2065 2524 2733 1932
Size: 133 459 209 3548
Fragments arranged by size:
3548 459 209 133
TatI w'GTAC_w
Cuts at: 708 3864 708
Size: 3156 1193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4317
Tth111I GACn'n_nGTC
Cuts at: 2146 2146
Size: 4349
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1844 1844
Size: 4349
XmaI C'CCGG_G
Cuts at: 591 1369 591
Size: 778 3571
XmnI GAAnn'nnTTC
Cuts at: 1255 1255
Size: 4349
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI