Restriction map for
pF1KB5813
(Circular) (Six-base) MAPSORT of: pF1KB5813.seq Check: 251 from: 1 to: 4535
pF1KB5813 4535 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 375 658 718 1220 1380 375
Size: 283 60 502 160 3530
Fragments arranged by size:
3530 502 283 160 60
AatII G_ACGT'C
Cuts at: 746 746
Size: 4535
AccI GT'mk_AC
Cuts at: 206 1573 206
Size: 1367 3168
Acc65I G'GTAC_C
Cuts at: 1551 1551
Size: 4535
AclI AA'CG_TT
Cuts at: 4375 4375
Size: 4535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 474 2360 2792 3539 3807 474
Size: 1886 432 747 268 1202
Fragments arranged by size:
1886 1202 747 432 268
AflIII A'CryG_T
Cuts at: 548 722 1103 1887 2991 3968 548
Size: 174 381 784 1104 977 1115
Fragments arranged by size:
1115 1104 977 784 381 174
AgeI A'CCGG_T
Cuts at: 2923 2923
Size: 4535
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 976 976
Size: 4535
AlwNI CAG_nnn'CTG
Cuts at: 798 1226 1840 3407 798
Size: 428 614 1567 1926
Fragments arranged by size:
1926 1567 614 428
ApaLI G'TGCA_C
Cuts at: 3305 3305
Size: 4535
ApoI r'AATT_y
Cuts at: 1539 3888 4144 1539
Size: 2349 256 1930
Fragments arranged by size:
2349 1930 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4535
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
AvaI C'yCGr_G
Cuts at: 316 407 526 1555 316
Size: 91 119 1029 3296
Fragments arranged by size:
3296 1029 119 91
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 535 568 535
Size: 33 4502
BamHI G'GATC_C
Cuts at: 1560 1560
Size: 4535
BanI G'GyrC_C
Cuts at: 646 1208 1392 1551 1965 2213 2248 646
Size: 562 184 159 414 248 35 2933
Fragments arranged by size:
2933 562 414 248 184 159 35
BanII G_rGCy'C
Cuts at: 437 451 606 946 1073 1157 1549 2579 437
Size: 14 155 340 127 84 392 1030 2393
Fragments arranged by size:
2393 1030 392 340 155 127 84 14
BbeI G_GCGC'C
Cuts at: 1969 2217 1969
Size: 248 4287
BbsI GAAGACnn'nnnn_
Cuts at: 738 738
Size: 4535
BbvCI CC'TCA_GC
Cuts at: 1337 1337
Size: 4535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 253 287 253
Size: 34 4501
BciVI GTATCCnnnnn_n'
Cuts at: 2433 3194 4535 2433
Size: 761 1341 2433
Fragments arranged by size:
2433 1341 761
BclI T'GATC_A
Cuts at: 2055 2055
Size: 4535
BglI GCCn_nnn'nGGC
Cuts at: 478 1206 1422 478
Size: 728 216 3591
Fragments arranged by size:
3591 728 216
BglII A'GATC_T
Cuts at: 1163 1428 2050 4087 1163
Size: 265 622 2037 1611
Fragments arranged by size:
2037 1611 622 265
BlpI GC'TnA_GC
Cuts at: 1647 1647
Size: 4535
Bme1580I G_kGCm'C
Cuts at: 649 722 1211 1397 2160 2253 3309 649
Size: 73 489 186 763 93 1056 1875
Fragments arranged by size:
1875 1056 763 489 186 93 73
BmrI ACTGGGnnnn_n'
Cuts at: 1140 1747 1828 2163 4307 1140
Size: 607 81 335 2144 1368
Fragments arranged by size:
2144 1368 607 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 4535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 435 606 1024 1478 388
Size: 47 171 418 454 3445
Fragments arranged by size:
3445 454 418 171 47
Bpu10I CC'TnA_GC
Cuts at: 1337 1337
Size: 4535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1711 2590 3082 3380 766
Size: 945 879 492 298 1921
Fragments arranged by size:
1921 945 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4239 4239
Size: 4535
BsaAI yAC'GTr
Cuts at: 723 1104 1888 723
Size: 381 784 3370
Fragments arranged by size:
3370 784 381
BsaBI GATnn'nnATC
Cuts at: 6 2049 4086 6
Size: 2043 2037 455
Fragments arranged by size:
2043 2037 455
BsaHI Gr'CG_yC
Cuts at: 743 1966 2214 2916 4412 743
Size: 1223 248 702 1496 866
Fragments arranged by size:
1496 1223 866 702 248
BsaWI w'CCGG_w
Cuts at: 1724 2245 2923 3197 3344 3755 1724
Size: 521 678 274 147 411 2504
Fragments arranged by size:
2504 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 579 609 579
Size: 30 4505
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 769 1126 1397 769
Size: 357 271 3907
Fragments arranged by size:
3907 357 271
BseYI C'CCAG_C
Cuts at: 901 1088 1292 1383 1457 1503 1960 2718 3295
Size: 187 204 91 74 46 457 758 577
Cuts at: 3295 3791 901
Size: 496 1645
Fragments arranged by size:
1645 758 577 496 457 204 187 91
74 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 657 496
Size: 161 4374
BsiEI CG_ry'CG
Cuts at: 104 302 1529 2077 2123 3331 104
Size: 198 1227 548 46 1208 1308
Fragments arranged by size:
1308 1227 1208 548 198 46
BsiHKAI G_wGCw'C
Cuts at: 437 946 1001 1073 1157 1549 2327 2517 3309
Size: 509 55 72 84 392 778 190 792
Cuts at: 3309 437
Size: 1663
Fragments arranged by size:
1663 792 778 509 392 190 84 72
55
Bsp1286I G_dGCh'C
Cuts at: 437 451 606 649 722 946 1001 1073 1157
Size: 14 155 43 73 224 55 72 84
Cuts at: 1157 1211 1397 1549 2160 2253 2327 2517 2579
Size: 54 186 152 611 93 74 190 62
Cuts at: 2579 3309 437
Size: 730 1663
Fragments arranged by size:
1663 730 611 224 190 186 155 152
93 84 74 73 72 62 55 54
43 14
BspEI T'CCGG_A
Cuts at: 3755 3755
Size: 4535
BspHI T'CATG_A
Cuts at: 3711 3711
Size: 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 254 375 658 718 1220 1270 1380 1585 1799
Size: 121 283 60 502 50 110 205 214
Cuts at: 1799 2101 2482 254
Size: 302 381 2307
Fragments arranged by size:
2307 502 381 302 283 214 205 121
110 60 50
BsrBI CCG'CTC
Cuts at: 50 2827 2881 4365 4530 50
Size: 2777 54 1484 165 55
Fragments arranged by size:
2777 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 205 1411 2447 205
Size: 1206 1036 2293
Fragments arranged by size:
2293 1206 1036
BsrFI r'CCGG_y
Cuts at: 301 2533 2714 2923 301
Size: 2232 181 209 1913
Fragments arranged by size:
2232 1913 209 181
BssSI C'ACGA_G
Cuts at: 2806 3164 2806
Size: 358 4177
BstAPI GCAn_nnn'nTGC
Cuts at: 1226 1912 1226
Size: 686 3849
BstBI TT'CG_AA
Cuts at: 2896 2896
Size: 4535
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4535
BstYI r'GATC_y
Cuts at: 1 1163 1428 1560 2027 2050 2385 2631 3643
Size: 1162 265 132 467 23 335 246 1012
Cuts at: 3643 3729 3741 4087 1
Size: 86 12 346 449
Fragments arranged by size:
1162 1012 467 449 346 335 265 246
132 86 23 12
BtgI C'CryG_G
Cuts at: 107 127 766 1136 1320 1420 107
Size: 20 639 370 184 100 3222
Fragments arranged by size:
3222 639 370 184 100 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2485 2666 2829 2485
Size: 181 163 4191
Fragments arranged by size:
4191 181 163
BtsI GCAGTG_nn'
Cuts at: 928 1362 1778 928
Size: 434 416 3685
Fragments arranged by size:
3685 434 416
DraI TTT'AAA
Cuts at: 1534 1534
Size: 4535
DraIII CAC_nnn'GTG
Cuts at: 660 660
Size: 4535
DrdI GACnn_nn'nnGTC
Cuts at: 2241 3099 2241
Size: 858 3677
EaeI y'GGCC_r
Cuts at: 299 359 389 470 479 769 1074 1423 1526
Size: 60 30 81 9 290 305 349 103
Cuts at: 1526 2120 2294 2685 2712 4057 299
Size: 594 174 391 27 1345 777
Fragments arranged by size:
1345 777 594 391 349 305 290 174
103 81 60 30 27 9
EagI C'GGCC_G
Cuts at: 299 1526 2120 299
Size: 1227 594 2714
Fragments arranged by size:
2714 1227 594
EarI CTCTTCn'nnn_
Cuts at: 140 188 442 526 2558 2768 140
Size: 48 254 84 2032 210 1907
Fragments arranged by size:
2032 1907 254 210 84 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 320 2743 3053 3199 4345 320
Size: 2423 310 146 1146 510
Fragments arranged by size:
2423 1146 510 310 146
EcoICRI GAG'CTC
Cuts at: 435 944 1071 1155 1547 435
Size: 509 127 84 392 3423
Fragments arranged by size:
3423 509 392 127 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 435 474 606 1024 1478 2360 2792 3539
Size: 47 39 132 418 454 882 432 747
Cuts at: 3539 3807 388
Size: 268 1116
Fragments arranged by size:
1116 882 747 454 432 418 268 132
47 39
EcoNI CCTnn'n_nnAGG
Cuts at: 585 991 585
Size: 406 4129
EcoO109I rG'GnC_Cy
Cuts at: 243 322 567 1507 1515 1674 243
Size: 79 245 940 8 159 3104
Fragments arranged by size:
3104 940 245 159 79 8
EcoRI G'AATT_C
Cuts at: 1539 1539
Size: 4535
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1038 2554 1038
Size: 1516 3019
FspI TGC'GCA
Cuts at: 691 2316 691
Size: 1625 2910
HaeII r_GCGC'y
Cuts at: 189 1969 2217 3239 4218 189
Size: 1780 248 1022 979 506
Fragments arranged by size:
1780 1022 979 506 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 619 651 2377 2407 2409 2439 619
Size: 32 1726 30 2 30 2715
Fragments arranged by size:
2715 1726 32 30 30 2
HincII GTy'rAC
Cuts at: 207 807 1574 207
Size: 600 767 3168
Fragments arranged by size:
3168 767 600
KasI G'GCGC_C
Cuts at: 1965 2213 1965
Size: 248 4287
KpnI G_GTAC'C
Cuts at: 1555 1555
Size: 4535
MluI A'CGCG_T
Cuts at: 3968 3968
Size: 4535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1358 3206 3390 1358
Size: 1848 184 2503
Fragments arranged by size:
2503 1848 184
MscI TGG'CCA
Cuts at: 361 472 481 771 2296 361
Size: 111 9 290 1525 2600
Fragments arranged by size:
2600 1525 290 111 9
MslI CAynn'nnrTG
Cuts at: 1102 2524 2651 1102
Size: 1422 127 2986
Fragments arranged by size:
2986 1422 127
MspA1I CmG'CkG
Cuts at: 267 468 716 905 1119 1223 1277 1498 1646
Size: 201 248 189 214 104 54 221 148
Cuts at: 1646 1960 2320 3333 3578 267
Size: 314 360 1013 245 1224
Fragments arranged by size:
1224 1013 360 314 248 245 221 214
201 189 148 104 54
NaeI GCC'GGC
Cuts at: 303 2716 303
Size: 2413 2122
NarI GG'CG_CC
Cuts at: 1966 2214 1966
Size: 248 4287
NcoI C'CATG_G
Cuts at: 107 766 1136 107
Size: 659 370 3506
Fragments arranged by size:
3506 659 370
NgoMIV G'CCGG_C
Cuts at: 301 2714 301
Size: 2413 2122
NspI r_CATG'y
Cuts at: 501 552 1588 2088 2995 501
Size: 51 1036 500 907 2041
Fragments arranged by size:
2041 1036 907 500 51
PasI CC'CwG_GG
Cuts at: 159 620 159
Size: 461 4074
PciI A'CATG_T
Cuts at: 548 2991 548
Size: 2443 2092
PflMI CCAn_nnn'nTGG
Cuts at: 660 660
Size: 4535
PfoI T'CCnGG_A
Cuts at: 1001 1001
Size: 4535
PmeI GTTT'AAAC
Cuts at: 1534 1534
Size: 4535
PmlI CAC'GTG
Cuts at: 723 1104 723
Size: 381 4154
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3674 3706 3674
Size: 32 4503
PpuMI rG'GwC_Cy
Cuts at: 243 322 1507 1515 243
Size: 79 1185 8 3263
Fragments arranged by size:
3263 1185 79 8
PstI C_TGCA'G
Cuts at: 185 715 863 871 965 1124 1222 1582 2267
Size: 530 148 8 94 159 98 360 685
Cuts at: 2267 185
Size: 2453
Fragments arranged by size:
2453 685 530 360 159 148 98 94
8
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4535
PvuII CAG'CTG
Cuts at: 267 468 905 1119 1223 1498 1960 2320 267
Size: 201 437 214 104 275 462 360 2482
Fragments arranged by size:
2482 462 437 360 275 214 201 104
RsrII CG'GwC_CG
Cuts at: 2730 2730
Size: 4535
SacI G_AGCT'C
Cuts at: 437 946 1073 1157 1549 437
Size: 509 127 84 392 3423
Fragments arranged by size:
3423 509 392 127 84
SalI G'TCGA_C
Cuts at: 205 1572 205
Size: 1367 3168
SanDI GG'GwC_CC
Cuts at: 322 1507 322
Size: 1185 3350
SapI GCTCTTCn'nnn_
Cuts at: 188 442 2558 2768 188
Size: 254 2116 210 1955
Fragments arranged by size:
2116 1955 254 210
SbfI CC_TGCA'GG
Cuts at: 871 1582 871
Size: 711 3824
ScaI AGT'ACT
Cuts at: 538 4052 538
Size: 3514 1021
SfcI C'TryA_G
Cuts at: 33 181 711 859 867 961 1120 1218 1578
Size: 148 530 148 8 94 159 98 360
Cuts at: 1578 2263 3256 3447 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 530 360 191 159 148
148 98 94 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 478 478
Size: 4535
SfoI GGC'GCC
Cuts at: 1967 2215 1967
Size: 248 4287
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4535
SmaI CCC'GGG
Cuts at: 318 409 528 1557 318
Size: 91 119 1029 3296
Fragments arranged by size:
3296 1029 119 91
SmlI C'TyrA_G
Cuts at: 781 1690 2605 3097 3359 781
Size: 909 915 492 262 1957
Fragments arranged by size:
1957 915 909 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4535
SphI G_CATG'C
Cuts at: 1588 2088 1588
Size: 500 4035
StuI AGG'CCT
Cuts at: 865 865
Size: 4535
StyI C'CwwG_G
Cuts at: 107 563 766 1136 1669 2023 107
Size: 456 203 370 533 354 2619
Fragments arranged by size:
2619 533 456 370 354 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 827 2118 2251 2710 2919 827
Size: 1291 133 459 209 2443
Fragments arranged by size:
2443 1291 459 209 133
TatI w'GTAC_w
Cuts at: 536 907 4050 536
Size: 371 3143 1021
Fragments arranged by size:
3143 1021 371
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1341 1373 4052 4084 1341
Size: 32 2679 32 1792
Fragments arranged by size:
2679 1792 32 32
Tth111I GACn'n_nGTC
Cuts at: 2332 2332
Size: 4535
XbaI T'CTAG_A
Cuts at: 1566 1566
Size: 4535
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2030 2030
Size: 4535
XmaI C'CCGG_G
Cuts at: 316 407 526 1555 316
Size: 91 119 1029 3296
Fragments arranged by size:
3296 1029 119 91
ZraI GAC'GTC
Cuts at: 744 744
Size: 4535
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AloI ApaI AscI AvrII
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PspOMI PspXI PsrI SacII SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI