Restriction map for
pF1KB5807
(Circular) (Six-base) MAPSORT of: pF1KB5807.seq Check: 3930 from: 1 to: 4529
pF1KB5807 4529 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 124 124
Size: 4529
AccI GT'mk_AC
Cuts at: 1567 1567
Size: 4529
Acc65I G'GTAC_C
Cuts at: 557 1545 557
Size: 988 3541
AclI AA'CG_TT
Cuts at: 1523 4369 1523
Size: 2846 1683
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 853 874 2354 2786 3533 3801 309
Size: 544 21 1480 432 747 268 1037
Fragments arranged by size:
1480 1037 747 544 432 268 21
AflIII A'CryG_T
Cuts at: 1881 2985 3962 1881
Size: 1104 977 2448
Fragments arranged by size:
2448 1104 977
AgeI A'CCGG_T
Cuts at: 2917 2917
Size: 4529
AhdI GACnn_n'nnGTC
Cuts at: 1196 1196
Size: 4529
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 590 590
Size: 4529
AlwNI CAG_nnn'CTG
Cuts at: 1128 1834 3401 1128
Size: 706 1567 2256
Fragments arranged by size:
2256 1567 706
ApaLI G'TGCA_C
Cuts at: 1471 3299 1471
Size: 1828 2701
ApoI r'AATT_y
Cuts at: 1533 3882 4138 1533
Size: 2349 256 1924
Fragments arranged by size:
2349 1924 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4529
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
AvaI C'yCGr_G
Cuts at: 1549 1549
Size: 4529
BamHI G'GATC_C
Cuts at: 1554 1554
Size: 4529
BanI G'GyrC_C
Cuts at: 557 1545 1959 2207 2242 557
Size: 988 414 248 35 2844
Fragments arranged by size:
2844 988 414 248 35
BanII G_rGCy'C
Cuts at: 157 1109 1543 2573 157
Size: 952 434 1030 2113
Fragments arranged by size:
2113 1030 952 434
BbeI G_GCGC'C
Cuts at: 1963 2211 1963
Size: 248 4281
BbsI GAAGACnn'nnnn_
Cuts at: 1327 1327
Size: 4529
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1492 1526 1492
Size: 34 4495
BciVI GTATCCnnnnn_n'
Cuts at: 2427 3188 4529 2427
Size: 761 1341 2427
Fragments arranged by size:
2427 1341 761
BclI T'GATC_A
Cuts at: 2049 2049
Size: 4529
BglI GCCn_nnn'nGGC
Cuts at: 691 691
Size: 4529
BglII A'GATC_T
Cuts at: 1097 2044 4081 1097
Size: 947 2037 1545
Fragments arranged by size:
2037 1545 947
BlpI GC'TnA_GC
Cuts at: 1153 1641 1153
Size: 488 4041
Bme1580I G_kGCm'C
Cuts at: 1475 2154 2247 3303 1475
Size: 679 93 1056 2701
Fragments arranged by size:
2701 1056 679 93
BmgBI CAC'GTC
Cuts at: 1317 1317
Size: 4529
BmrI ACTGGGnnnn_n'
Cuts at: 1389 1741 1822 2157 4301 1389
Size: 352 81 335 2144 1617
Fragments arranged by size:
2144 1617 352 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2896 2896
Size: 4529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 174 535 832 1150 1192 1204 1351 1384
Size: 51 361 297 318 42 12 147 33
Cuts at: 1384 1422 123
Size: 38 3230
Fragments arranged by size:
3230 361 318 297 147 51 42 38
33 12
Bpu10I CC'TnA_GC
Cuts at: 946 946
Size: 4529
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1705 2584 3076 3374 1705
Size: 879 492 298 2860
Fragments arranged by size:
2860 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1183 4233 1183
Size: 3050 1479
BsaAI yAC'GTr
Cuts at: 1882 1882
Size: 4529
BsaBI GATnn'nnATC
Cuts at: 6 2043 4080 6
Size: 2037 2037 455
Fragments arranged by size:
2037 2037 455
BsaHI Gr'CG_yC
Cuts at: 1376 1960 2208 2910 4406 1376
Size: 584 248 702 1496 1499
Fragments arranged by size:
1499 1496 702 584 248
BsaWI w'CCGG_w
Cuts at: 1718 2239 2917 3191 3338 3749 1718
Size: 521 678 274 147 411 2498
Fragments arranged by size:
2498 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1372 1402 1372
Size: 30 4499
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 192 270 532 853 883 886 1054 1201
Size: 66 78 262 321 30 3 168 147
Cuts at: 1201 1264 1377 1380 1392 1425 126
Size: 63 113 3 12 33 3230
Fragments arranged by size:
3230 321 262 168 147 113 78 66
63 33 30 12 3 3
BseYI C'CCAG_C
Cuts at: 235 1148 1262 1954 2712 3289 3785 235
Size: 913 114 692 758 577 496 979
Fragments arranged by size:
979 913 758 692 577 496 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 914 1099 914
Size: 185 4344
BsiEI CG_ry'CG
Cuts at: 104 480 2071 2117 3325 104
Size: 376 1591 46 1208 1308
Fragments arranged by size:
1591 1308 1208 376 46
BsiHKAI G_wGCw'C
Cuts at: 513 1109 1475 1543 2321 2511 3303 513
Size: 596 366 68 778 190 792 1739
Fragments arranged by size:
1739 792 778 596 366 190 68
BsmI GAATG_Cn'
Cuts at: 885 953 885
Size: 68 4461
Bsp1286I G_dGCh'C
Cuts at: 157 513 1109 1475 1543 2154 2247 2321 2511
Size: 356 596 366 68 611 93 74 190
Cuts at: 2511 2573 3303 157
Size: 62 730 1383
Fragments arranged by size:
1383 730 611 596 366 356 190 93
74 68 62
BspEI T'CCGG_A
Cuts at: 3749 3749
Size: 4529
BspHI T'CATG_A
Cuts at: 3705 3705
Size: 4529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 124 743 1494 1579 1793 2095 2476 124
Size: 619 751 85 214 302 381 2177
Fragments arranged by size:
2177 751 619 381 302 214 85
BsrBI CCG'CTC
Cuts at: 50 1085 2821 2875 4359 4524 50
Size: 1035 1736 54 1484 165 55
Fragments arranged by size:
1736 1484 1035 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2441 2441
Size: 4529
BsrFI r'CCGG_y
Cuts at: 2527 2708 2917 2527
Size: 181 209 4139
Fragments arranged by size:
4139 209 181
BssSI C'ACGA_G
Cuts at: 865 1498 2800 3158 865
Size: 633 1302 358 2236
Fragments arranged by size:
2236 1302 633 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1906 1906
Size: 4529
BstBI TT'CG_AA
Cuts at: 2890 2890
Size: 4529
BstEII G'GTnAC_C
Cuts at: 456 456
Size: 4529
BstXI CCAn_nnnn'nTGG
Cuts at: 901 1464 901
Size: 563 3966
BstYI r'GATC_y
Cuts at: 1 545 1097 1223 1304 1554 2021 2044 2379
Size: 544 552 126 81 250 467 23 335
Cuts at: 2379 2625 3637 3723 3735 4081 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 552 544 467 449 346 335 250
246 126 86 81 23 12
Bsu36I CC'TnA_GG
Cuts at: 622 622
Size: 4529
BtgI C'CryG_G
Cuts at: 695 695
Size: 4529
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2479 2660 2823 2479
Size: 181 163 4185
Fragments arranged by size:
4185 181 163
BtsI GCAGTG_nn'
Cuts at: 453 1772 453
Size: 1319 3210
DraI TTT'AAA
Cuts at: 1528 1528
Size: 4529
DrdI GACnn_nn'nnGTC
Cuts at: 2235 3093 2235
Size: 858 3671
EaeI y'GGCC_r
Cuts at: 212 692 797 1058 2114 2288 2679 2706 4051
Size: 480 105 261 1056 174 391 27 1345
Cuts at: 4051 212
Size: 690
Fragments arranged by size:
1345 1056 690 480 391 261 174 105
27
EagI C'GGCC_G
Cuts at: 2114 2114
Size: 4529
EarI CTCTTCn'nnn_
Cuts at: 2552 2762 2552
Size: 210 4319
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2737 3047 3193 4339 2737
Size: 310 146 1146 2927
Fragments arranged by size:
2927 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1107 1541 1107
Size: 434 4095
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 174 309 535 832 853 874 1150 1192
Size: 51 135 226 297 21 21 276 42
Cuts at: 1192 1204 1351 1384 1422 2354 2786 3533 3801
Size: 12 147 33 38 932 432 747 268
Cuts at: 3801 123
Size: 851
Fragments arranged by size:
932 851 747 432 297 276 268 226
147 135 51 42 38 33 21 21
12
EcoNI CCTnn'n_nnAGG
Cuts at: 499 835 1363 499
Size: 336 528 3665
Fragments arranged by size:
3665 528 336
EcoO109I rG'GnC_Cy
Cuts at: 221 618 1506 1668 221
Size: 397 888 162 3082
Fragments arranged by size:
3082 888 397 162
EcoRI G'AATT_C
Cuts at: 1533 1533
Size: 4529
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2548 2548
Size: 4529
FspI TGC'GCA
Cuts at: 739 772 2310 739
Size: 33 1538 2958
Fragments arranged by size:
2958 1538 33
HaeII r_GCGC'y
Cuts at: 1963 2211 3233 4212 1963
Size: 248 1022 979 2280
Fragments arranged by size:
2280 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1129 1161 1182 1214 1417 1449 2371 2401 2403
Size: 32 21 32 203 32 922 30 2
Cuts at: 2403 2433 1129
Size: 30 3225
Fragments arranged by size:
3225 922 203 32 32 32 30 30
21 2
HincII GTy'rAC
Cuts at: 1568 1568
Size: 4529
KasI G'GCGC_C
Cuts at: 1959 2207 1959
Size: 248 4281
KpnI G_GTAC'C
Cuts at: 561 1549 561
Size: 988 3541
MluI A'CGCG_T
Cuts at: 3962 3962
Size: 4529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3200 3384 3200
Size: 184 4345
MscI TGG'CCA
Cuts at: 799 1060 2290 799
Size: 261 1230 3038
Fragments arranged by size:
3038 1230 261
MslI CAynn'nnrTG
Cuts at: 745 1468 2518 2645 745
Size: 723 1050 127 2629
Fragments arranged by size:
2629 1050 723 127
MspA1I CmG'CkG
Cuts at: 570 697 1215 1257 1271 1640 1954 2314 3327
Size: 127 518 42 14 369 314 360 1013
Cuts at: 3327 3572 570
Size: 245 1527
Fragments arranged by size:
1527 1013 518 369 360 314 245 127
42 14
NaeI GCC'GGC
Cuts at: 2710 2710
Size: 4529
NarI GG'CG_CC
Cuts at: 1960 2208 1960
Size: 248 4281
NgoMIV G'CCGG_C
Cuts at: 2708 2708
Size: 4529
NspI r_CATG'y
Cuts at: 1212 1582 2082 2989 1212
Size: 370 500 907 2752
Fragments arranged by size:
2752 907 500 370
PciI A'CATG_T
Cuts at: 2985 2985
Size: 4529
PfoI T'CCnGG_A
Cuts at: 1226 1226
Size: 4529
PmeI GTTT'AAAC
Cuts at: 1528 1528
Size: 4529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1006 1038 3668 3700 1006
Size: 32 2630 32 1835
Fragments arranged by size:
2630 1835 32 32
PpuMI rG'GwC_Cy
Cuts at: 221 618 1506 221
Size: 397 888 3244
Fragments arranged by size:
3244 888 397
PshAI GACnn'nnGTC
Cuts at: 593 593
Size: 4529
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1282 1314 1282
Size: 32 4497
PstI C_TGCA'G
Cuts at: 121 674 1145 1576 2261 121
Size: 553 471 431 685 2389
Fragments arranged by size:
2389 685 553 471 431
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4529
PvuII CAG'CTG
Cuts at: 1215 1257 1954 2314 1215
Size: 42 697 360 3430
Fragments arranged by size:
3430 697 360 42
RsrII CG'GwC_CG
Cuts at: 2724 2724
Size: 4529
SacI G_AGCT'C
Cuts at: 1109 1543 1109
Size: 434 4095
SacII CC_GC'GG
Cuts at: 698 698
Size: 4529
SalI G'TCGA_C
Cuts at: 1566 1566
Size: 4529
SapI GCTCTTCn'nnn_
Cuts at: 2552 2762 2552
Size: 210 4319
SbfI CC_TGCA'GG
Cuts at: 1576 1576
Size: 4529
ScaI AGT'ACT
Cuts at: 4046 4046
Size: 4529
SfcI C'TryA_G
Cuts at: 33 117 591 670 1141 1572 2257 3250 3441
Size: 84 474 79 471 431 685 993 191
Cuts at: 3441 33
Size: 1121
Fragments arranged by size:
1121 993 685 474 471 431 191 84
79
SfoI GGC'GCC
Cuts at: 1961 2209 1961
Size: 248 4281
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4529
SmaI CCC'GGG
Cuts at: 1551 1551
Size: 4529
SmlI C'TyrA_G
Cuts at: 1684 2599 3091 3353 1684
Size: 915 492 262 2860
Fragments arranged by size:
2860 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4529
SphI G_CATG'C
Cuts at: 1212 1582 2082 1212
Size: 370 500 3659
Fragments arranged by size:
3659 500 370
StyI C'CwwG_G
Cuts at: 381 1457 1663 2017 381
Size: 1076 206 354 2893
Fragments arranged by size:
2893 1076 354 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 385 889 1510 2112 2245 2704 2913 385
Size: 504 621 602 133 459 209 2001
Fragments arranged by size:
2001 621 602 504 459 209 133
TatI w'GTAC_w
Cuts at: 1298 4044 1298
Size: 2746 1783
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1372 1404 4046 4078 1372
Size: 32 2642 32 1823
Fragments arranged by size:
2642 1823 32 32
Tth111I GACn'n_nGTC
Cuts at: 2326 2326
Size: 4529
XbaI T'CTAG_A
Cuts at: 1560 1560
Size: 4529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 531 807 1071 1125 1234 1402 2024 531
Size: 276 264 54 109 168 622 3036
Fragments arranged by size:
3036 622 276 264 168 109 54
XmaI C'CCGG_G
Cuts at: 1549 1549
Size: 4529
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PshAI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BmtI BsiWI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PsiI PspOMI PspXI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI