Restriction map for
pF1KB5801
(Circular) (Six-base) MAPSORT of: pF1KB5801.seq Check: 6915 from: 1 to: 4226
pF1KB5801 4226 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 1264 1264
Size: 4226
Acc65I G'GTAC_C
Cuts at: 1242 1242
Size: 4226
AclI AA'CG_TT
Cuts at: 4066 4066
Size: 4226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2483 3230 3498 2051
Size: 432 747 268 2779
Fragments arranged by size:
2779 747 432 268
AflIII A'CryG_T
Cuts at: 722 1578 2682 3659 722
Size: 856 1104 977 1289
Fragments arranged by size:
1289 1104 977 856
AgeI A'CCGG_T
Cuts at: 2614 2614
Size: 4226
AlwNI CAG_nnn'CTG
Cuts at: 1100 1531 3098 1100
Size: 431 1567 2228
Fragments arranged by size:
2228 1567 431
ApaLI G'TGCA_C
Cuts at: 2996 2996
Size: 4226
ApoI r'AATT_y
Cuts at: 163 396 444 1204 1230 3579 3835 163
Size: 233 48 760 26 2349 256 554
Fragments arranged by size:
2349 760 554 256 233 48 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 4226
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
AvaI C'yCGr_G
Cuts at: 600 670 878 1246 600
Size: 70 208 368 3580
Fragments arranged by size:
3580 368 208 70
BamHI G'GATC_C
Cuts at: 665 1050 1251 665
Size: 385 201 3640
Fragments arranged by size:
3640 385 201
BanI G'GyrC_C
Cuts at: 1242 1656 1904 1939 1242
Size: 414 248 35 3529
Fragments arranged by size:
3529 414 248 35
BanII G_rGCy'C
Cuts at: 639 910 1240 2270 639
Size: 271 330 1030 2595
Fragments arranged by size:
2595 1030 330 271
BbeI G_GCGC'C
Cuts at: 1660 1908 1660
Size: 248 3978
BbsI GAAGACnn'nnnn_
Cuts at: 549 748 940 549
Size: 199 192 3835
Fragments arranged by size:
3835 199 192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 250 284 805 839 1067 1101 250
Size: 34 521 34 228 34 3375
Fragments arranged by size:
3375 521 228 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2124 2885 4226 2124
Size: 761 1341 2124
Fragments arranged by size:
2124 1341 761
BclI T'GATC_A
Cuts at: 1746 1746
Size: 4226
BglII A'GATC_T
Cuts at: 135 1741 3778 135
Size: 1606 2037 583
Fragments arranged by size:
2037 1606 583
BlpI GC'TnA_GC
Cuts at: 1338 1338
Size: 4226
Bme1580I G_kGCm'C
Cuts at: 1851 1944 3000 1851
Size: 93 1056 3077
Fragments arranged by size:
3077 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 823 913 1139 1438 1519 1854 3998 823
Size: 90 226 299 81 335 2144 1051
Fragments arranged by size:
2144 1051 335 299 226 90 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 4226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 777 777
Size: 4226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 2281 2773 3071 1402
Size: 879 492 298 2557
Fragments arranged by size:
2557 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 573 3930 573
Size: 3357 869
BsaAI yAC'GTr
Cuts at: 1579 1579
Size: 4226
BsaBI GATnn'nnATC
Cuts at: 6 1740 3777 6
Size: 1734 2037 455
Fragments arranged by size:
2037 1734 455
BsaHI Gr'CG_yC
Cuts at: 1657 1905 2607 4103 1657
Size: 248 702 1496 1780
Fragments arranged by size:
1780 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1415 1936 2614 2888 3035 3446 1415
Size: 521 678 274 147 411 2195
Fragments arranged by size:
2195 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 536 566 624 654 536
Size: 30 58 30 4108
Fragments arranged by size:
4108 58 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 712 715 718 862 865 712
Size: 3 3 144 3 4073
Fragments arranged by size:
4073 144 3 3 3
BseYI C'CCAG_C
Cuts at: 974 1651 2409 2986 3482 974
Size: 677 758 577 496 1718
Fragments arranged by size:
1718 758 677 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 318
Size: 4226
BsiEI CG_ry'CG
Cuts at: 104 1768 1814 3022 104
Size: 1664 46 1208 1308
Fragments arranged by size:
1664 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 639 1240 2018 2208 3000 639
Size: 601 778 190 792 1865
Fragments arranged by size:
1865 792 778 601 190
Bsp1286I G_dGCh'C
Cuts at: 639 910 1240 1851 1944 2018 2208 2270 3000
Size: 271 330 611 93 74 190 62 730
Cuts at: 3000 639
Size: 1865
Fragments arranged by size:
1865 730 611 330 271 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3446 3446
Size: 4226
BspHI T'CATG_A
Cuts at: 3402 3402
Size: 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 782 1276 1490 1792 2173 782
Size: 494 214 302 381 2835
Fragments arranged by size:
2835 494 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2518 2572 4056 4221 50
Size: 2468 54 1484 165 55
Fragments arranged by size:
2468 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 494 1062 2138 494
Size: 568 1076 2582
Fragments arranged by size:
2582 1076 568
BsrFI r'CCGG_y
Cuts at: 1103 2224 2405 2614 1103
Size: 1121 181 209 2715
Fragments arranged by size:
2715 1121 209 181
BssSI C'ACGA_G
Cuts at: 252 2497 2855 252
Size: 2245 358 1623
Fragments arranged by size:
2245 1623 358
BstAPI GCAn_nnn'nTGC
Cuts at: 296 493 1100 1603 296
Size: 197 607 503 2919
Fragments arranged by size:
2919 607 503 197
BstBI TT'CG_AA
Cuts at: 1011 2587 1011
Size: 1576 2650
BstXI CCAn_nnnn'nTGG
Cuts at: 974 997 974
Size: 23 4203
BstYI r'GATC_y
Cuts at: 1 135 665 1050 1251 1718 1741 2076 2322
Size: 134 530 385 201 467 23 335 246
Cuts at: 2322 3334 3420 3432 3778 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 530 467 449 385 346 335 246
201 134 86 23 12
BtgI C'CryG_G
Cuts at: 107 188 537 692 746 107
Size: 81 349 155 54 3587
Fragments arranged by size:
3587 349 155 81 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 340 2176 2357 2520 340
Size: 1836 181 163 2046
Fragments arranged by size:
2046 1836 181 163
BtsI GCAGTG_nn'
Cuts at: 1469 1469
Size: 4226
DraI TTT'AAA
Cuts at: 467 1225 467
Size: 758 3468
DrdI GACnn_nn'nnGTC
Cuts at: 1932 2790 1932
Size: 858 3368
EaeI y'GGCC_r
Cuts at: 1811 1985 2376 2403 3748 1811
Size: 174 391 27 1345 2289
Fragments arranged by size:
2289 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1811 1811
Size: 4226
EarI CTCTTCn'nnn_
Cuts at: 704 1022 2249 2459 704
Size: 318 1227 210 2471
Fragments arranged by size:
2471 1227 318 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2434 2744 2890 4036 2434
Size: 310 146 1146 2624
Fragments arranged by size:
2624 1146 310 146
EcoICRI GAG'CTC
Cuts at: 637 1238 637
Size: 601 3625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 777 2051 2483 3230 3498 777
Size: 1274 432 747 268 1505
Fragments arranged by size:
1505 1274 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1076 1198 1076
Size: 122 4104
EcoO109I rG'GnC_Cy
Cuts at: 284 659 674 1134 1365 284
Size: 375 15 460 231 3145
Fragments arranged by size:
3145 460 375 231 15
EcoRI G'AATT_C
Cuts at: 396 1230 396
Size: 834 3392
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 171 508 2245 171
Size: 337 1737 2152
Fragments arranged by size:
2152 1737 337
FspI TGC'GCA
Cuts at: 2007 2007
Size: 4226
HaeII r_GCGC'y
Cuts at: 1660 1908 2930 3909 1660
Size: 248 1022 979 1977
Fragments arranged by size:
1977 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 400 432 536 556 568 588 624 656 2068
Size: 32 104 20 12 20 36 32 1412
Cuts at: 2068 2098 2100 2130 400
Size: 30 2 30 2496
Fragments arranged by size:
2496 1412 104 36 32 32 30 30
20 20 12 2
HincII GTy'rAC
Cuts at: 1265 1265
Size: 4226
HindIII A'AGCT_T
Cuts at: 519 519
Size: 4226
KasI G'GCGC_C
Cuts at: 1656 1904 1656
Size: 248 3978
KpnI G_GTAC'C
Cuts at: 1246 1246
Size: 4226
MluI A'CGCG_T
Cuts at: 3659 3659
Size: 4226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 437 587 836 2897 3081 437
Size: 150 249 2061 184 1582
Fragments arranged by size:
2061 1582 249 184 150
MscI TGG'CCA
Cuts at: 1987 1987
Size: 4226
MslI CAynn'nnrTG
Cuts at: 727 2215 2342 727
Size: 1488 127 2611
Fragments arranged by size:
2611 1488 127
MspA1I CmG'CkG
Cuts at: 1337 1651 2011 3024 3269 1337
Size: 314 360 1013 245 2294
Fragments arranged by size:
2294 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1105 2407 1105
Size: 1302 2924
NarI GG'CG_CC
Cuts at: 1657 1905 1657
Size: 248 3978
NcoI C'CATG_G
Cuts at: 107 188 692 107
Size: 81 504 3641
Fragments arranged by size:
3641 504 81
NgoMIV G'CCGG_C
Cuts at: 1103 2405 1103
Size: 1302 2924
NspI r_CATG'y
Cuts at: 726 1180 1279 1779 2686 726
Size: 454 99 500 907 2266
Fragments arranged by size:
2266 907 500 454 99
PciI A'CATG_T
Cuts at: 722 2682 722
Size: 1960 2266
PfoI T'CCnGG_A
Cuts at: 613 661 613
Size: 48 4178
PmeI GTTT'AAAC
Cuts at: 1225 1225
Size: 4226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 4194
PpuMI rG'GwC_Cy
Cuts at: 284 659 674 1134 284
Size: 375 15 460 3376
Fragments arranged by size:
3376 460 375 15
PshAI GACnn'nnGTC
Cuts at: 1132 1132
Size: 4226
PstI C_TGCA'G
Cuts at: 263 871 961 1096 1273 1958 263
Size: 608 90 135 177 685 2531
Fragments arranged by size:
2531 685 608 177 135 90
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4226
PvuII CAG'CTG
Cuts at: 1651 2011 1651
Size: 360 3866
RsrII CG'GwC_CG
Cuts at: 2421 2421
Size: 4226
SacI G_AGCT'C
Cuts at: 639 1240 639
Size: 601 3625
SalI G'TCGA_C
Cuts at: 1263 1263
Size: 4226
SanDI GG'GwC_CC
Cuts at: 674 674
Size: 4226
SapI GCTCTTCn'nnn_
Cuts at: 2249 2459 2249
Size: 210 4016
SbfI CC_TGCA'GG
Cuts at: 1273 1273
Size: 4226
ScaI AGT'ACT
Cuts at: 3743 3743
Size: 4226
SexAI A'CCwGG_T
Cuts at: 649 649
Size: 4226
SfcI C'TryA_G
Cuts at: 33 259 279 867 957 1092 1269 1954 2947
Size: 226 20 588 90 135 177 685 993
Cuts at: 2947 3138 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 588 226 191 177 135
90 20
SfoI GGC'GCC
Cuts at: 1658 1906 1658
Size: 248 3978
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
SmaI CCC'GGG
Cuts at: 602 1248 602
Size: 646 3580
SmlI C'TyrA_G
Cuts at: 878 1381 2296 2788 3050 878
Size: 503 915 492 262 2054
Fragments arranged by size:
2054 915 503 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4226
SphI G_CATG'C
Cuts at: 1180 1279 1779 1180
Size: 99 500 3627
Fragments arranged by size:
3627 500 99
StyI C'CwwG_G
Cuts at: 107 188 692 1360 1714 107
Size: 81 504 668 354 2619
Fragments arranged by size:
2619 668 504 354 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1809 1942 2401 2610 1809
Size: 133 459 209 3425
Fragments arranged by size:
3425 459 209 133
TatI w'GTAC_w
Cuts at: 719 3741 719
Size: 3022 1204
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3743 3775 3743
Size: 32 4194
Tth111I GACn'n_nGTC
Cuts at: 2023 2023
Size: 4226
XbaI T'CTAG_A
Cuts at: 1257 1257
Size: 4226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1721 1721
Size: 4226
XhoI C'TCGA_G
Cuts at: 878 878
Size: 4226
XmaI C'CCGG_G
Cuts at: 600 1246 600
Size: 646 3580
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PsiI PspOMI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI