Restriction map for
pF1KB5766
(Circular) (Six-base) MAPSORT of: pF1KB5766.seq Check: 3875 from: 1 to: 5354
pF1KB5766 5354 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 520 630 520
Size: 110 5244
AccI GT'mk_AC
Cuts at: 2392 2392
Size: 5354
Acc65I G'GTAC_C
Cuts at: 2370 2370
Size: 5354
AclI AA'CG_TT
Cuts at: 5194 5194
Size: 5354
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 503 692 1772 2116 2233 2269 3179 3611
Size: 306 189 1080 344 117 36 910 432
Cuts at: 3611 4358 4626 197
Size: 747 268 925
Fragments arranged by size:
1080 925 910 747 432 344 306 268
189 117 36
AflIII A'CryG_T
Cuts at: 2706 3810 4787 2706
Size: 1104 977 3273
Fragments arranged by size:
3273 1104 977
AgeI A'CCGG_T
Cuts at: 3742 3742
Size: 5354
AleI CACnn'nnGTG
Cuts at: 1445 2335 1445
Size: 890 4464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 806 806
Size: 5354
AlwNI CAG_nnn'CTG
Cuts at: 556 1611 1750 2070 2094 2659 4226 556
Size: 1055 139 320 24 565 1567 1684
Fragments arranged by size:
1684 1567 1055 565 320 139 24
ApaLI G'TGCA_C
Cuts at: 4124 4124
Size: 5354
ApoI r'AATT_y
Cuts at: 1597 1894 2358 4707 4963 1597
Size: 297 464 2349 256 1988
Fragments arranged by size:
2349 1988 464 297 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5354
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
AvaI C'yCGr_G
Cuts at: 1169 2374 1169
Size: 1205 4149
BamHI G'GATC_C
Cuts at: 2379 2379
Size: 5354
BanI G'GyrC_C
Cuts at: 2370 2784 3032 3067 2370
Size: 414 248 35 4657
Fragments arranged by size:
4657 414 248 35
BanII G_rGCy'C
Cuts at: 1974 2114 2368 3398 1974
Size: 140 254 1030 3930
Fragments arranged by size:
3930 1030 254 140
BbeI G_GCGC'C
Cuts at: 2788 3036 2788
Size: 248 5106
BbsI GAAGACnn'nnnn_
Cuts at: 1822 1822
Size: 5354
BbvCI CC'TCA_GC
Cuts at: 2087 2087
Size: 5354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5320
BciVI GTATCCnnnnn_n'
Cuts at: 3252 4013 5354 3252
Size: 761 1341 3252
Fragments arranged by size:
3252 1341 761
BclI T'GATC_A
Cuts at: 588 1311 1548 2874 588
Size: 723 237 1326 3068
Fragments arranged by size:
3068 1326 723 237
BglII A'GATC_T
Cuts at: 2869 4906 2869
Size: 2037 3317
BlpI GC'TnA_GC
Cuts at: 2466 2466
Size: 5354
Bme1580I G_kGCm'C
Cuts at: 2979 3072 4128 2979
Size: 93 1056 4205
Fragments arranged by size:
4205 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2566 2647 2982 5126 2566
Size: 81 335 2144 2794
Fragments arranged by size:
2794 2144 335 81
BmtI G_CTAG'C
Cuts at: 2191 2191
Size: 5354
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1641 3721 1641
Size: 2080 3274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 497 1190 1910 2203 269
Size: 228 693 720 293 3420
Fragments arranged by size:
3420 720 693 293 228
Bpu10I CC'TnA_GC
Cuts at: 1130 2087 1130
Size: 957 4397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2530 3409 3901 4199 2530
Size: 879 492 298 3685
Fragments arranged by size:
3685 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1954 5058 1954
Size: 3104 2250
BsaAI yAC'GTr
Cuts at: 2707 2707
Size: 5354
BsaBI GATnn'nnATC
Cuts at: 6 2868 4905 6
Size: 2862 2037 455
Fragments arranged by size:
2862 2037 455
BsaHI Gr'CG_yC
Cuts at: 2785 3033 3735 5231 2785
Size: 248 702 1496 2908
Fragments arranged by size:
2908 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2543 3064 3742 4016 4163 4574 2543
Size: 521 678 274 147 411 3323
Fragments arranged by size:
3323 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2038 2068 2038
Size: 30 5324
BseYI C'CCAG_C
Cuts at: 549 1027 1517 1848 2779 3537 4114 4610 549
Size: 478 490 331 931 758 577 496 1293
Fragments arranged by size:
1293 931 758 577 496 490 478 331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 2359 608
Size: 1751 3603
BsiEI CG_ry'CG
Cuts at: 104 2896 2942 4150 104
Size: 2792 46 1208 1308
Fragments arranged by size:
2792 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2114 2368 3146 3336 4128 2114
Size: 254 778 190 792 3340
Fragments arranged by size:
3340 792 778 254 190
BsmI GAATG_Cn'
Cuts at: 807 807
Size: 5354
BsmBI CGTCTCn'nnnn_
Cuts at: 1657 1657
Size: 5354
Bsp1286I G_dGCh'C
Cuts at: 1974 2114 2368 2979 3072 3146 3336 3398 4128
Size: 140 254 611 93 74 190 62 730
Cuts at: 4128 1974
Size: 3200
Fragments arranged by size:
3200 730 611 254 190 140 93 74
62
BspEI T'CCGG_A
Cuts at: 4574 4574
Size: 5354
BspHI T'CATG_A
Cuts at: 1149 4530 1149
Size: 3381 1973
BspMI ACCTGCnnnn'nnnn_
Cuts at: 520 630 2404 2618 2920 3301 520
Size: 110 1774 214 302 381 2573
Fragments arranged by size:
2573 1774 381 302 214 110
BsrBI CCG'CTC
Cuts at: 50 3646 3700 5184 5349 50
Size: 3596 54 1484 165 55
Fragments arranged by size:
3596 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 184 400 1286 2018 3266 184
Size: 216 886 732 1248 2272
Fragments arranged by size:
2272 1248 886 732 216
BsrFI r'CCGG_y
Cuts at: 3352 3533 3742 3352
Size: 181 209 4964
Fragments arranged by size:
4964 209 181
BsrGI T'GTAC_A
Cuts at: 1949 1949
Size: 5354
BssSI C'ACGA_G
Cuts at: 3625 3983 3625
Size: 358 4996
BstAPI GCAn_nnn'nTGC
Cuts at: 2731 2731
Size: 5354
BstBI TT'CG_AA
Cuts at: 3715 3715
Size: 5354
BstXI CCAn_nnnn'nTGG
Cuts at: 2232 2232
Size: 5354
BstYI r'GATC_y
Cuts at: 1 2379 2846 2869 3204 3450 4462 4548 4560
Size: 2378 467 23 335 246 1012 86 12
Cuts at: 4560 4906 1
Size: 346 449
Fragments arranged by size:
2378 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 569 1567 2120 569
Size: 998 553 3803
Fragments arranged by size:
3803 998 553
BtgI C'CryG_G
Cuts at: 851 1496 1941 851
Size: 645 445 4264
Fragments arranged by size:
4264 645 445
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3304 3485 3648 3304
Size: 181 163 5010
Fragments arranged by size:
5010 181 163
BtsI GCAGTG_nn'
Cuts at: 2597 2597
Size: 5354
ClaI AT'CG_AT
Cuts at: 415 415
Size: 5354
DraI TTT'AAA
Cuts at: 581 1182 2139 2353 581
Size: 601 957 214 3582
Fragments arranged by size:
3582 957 601 214
DrdI GACnn_nn'nnGTC
Cuts at: 3060 3918 3060
Size: 858 4496
EaeI y'GGCC_r
Cuts at: 2939 3113 3504 3531 4876 2939
Size: 174 391 27 1345 3417
Fragments arranged by size:
3417 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2939 2939
Size: 5354
EarI CTCTTCn'nnn_
Cuts at: 367 968 1748 1952 3377 3587 367
Size: 601 780 204 1425 210 2134
Fragments arranged by size:
2134 1425 780 601 210 204
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3562 3872 4018 5164 3562
Size: 310 146 1146 3752
Fragments arranged by size:
3752 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2112 2366 2112
Size: 254 5100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 269 497 503 692 1190 1772 1910 2116
Size: 72 228 6 189 498 582 138 206
Cuts at: 2116 2203 2233 2269 3179 3611 4358 4626 197
Size: 87 30 36 910 432 747 268 925
Fragments arranged by size:
925 910 747 582 498 432 268 228
206 189 138 87 72 36 30 6
EcoO109I rG'GnC_Cy
Cuts at: 2493 2493
Size: 5354
EcoRI G'AATT_C
Cuts at: 1894 2358 1894
Size: 464 4890
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1981 3373 1981
Size: 1392 3962
FspI TGC'GCA
Cuts at: 3135 3135
Size: 5354
HaeII r_GCGC'y
Cuts at: 2315 2788 3036 4058 5037 2315
Size: 473 248 1022 979 2632
Fragments arranged by size:
2632 1022 979 473 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 526 558 868 900 1068 1100 1246 1264 1278
Size: 32 310 32 168 32 146 18 14
Cuts at: 1278 1296 3196 3226 3228 3258 526
Size: 18 1900 30 2 30 2622
Fragments arranged by size:
2622 1900 310 168 146 32 32 32
30 30 18 18 14 2
HincII GTy'rAC
Cuts at: 230 1671 2393 230
Size: 1441 722 3191
Fragments arranged by size:
3191 1441 722
HindIII A'AGCT_T
Cuts at: 1904 1904
Size: 5354
KasI G'GCGC_C
Cuts at: 2784 3032 2784
Size: 248 5106
KpnI G_GTAC'C
Cuts at: 2374 2374
Size: 5354
MluI A'CGCG_T
Cuts at: 4787 4787
Size: 5354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 857 4025 4209 857
Size: 3168 184 2002
Fragments arranged by size:
3168 2002 184
MscI TGG'CCA
Cuts at: 3115 3115
Size: 5354
MslI CAynn'nnrTG
Cuts at: 1445 2335 3343 3470 1445
Size: 890 1008 127 3329
Fragments arranged by size:
3329 1008 890 127
MspA1I CmG'CkG
Cuts at: 1317 1537 1848 2211 2465 2779 3139 4152 4397
Size: 220 311 363 254 314 360 1013 245
Cuts at: 4397 1317
Size: 2274
Fragments arranged by size:
2274 1013 363 360 314 311 254 245
220
NaeI GCC'GGC
Cuts at: 3535 3535
Size: 5354
NarI GG'CG_CC
Cuts at: 2785 3033 2785
Size: 248 5106
NcoI C'CATG_G
Cuts at: 851 1496 851
Size: 645 4709
NdeI CA'TA_TG
Cuts at: 2151 2304 2151
Size: 153 5201
NgoMIV G'CCGG_C
Cuts at: 3533 3533
Size: 5354
NheI G'CTAG_C
Cuts at: 2187 2187
Size: 5354
NspI r_CATG'y
Cuts at: 2407 2907 3814 2407
Size: 500 907 3947
Fragments arranged by size:
3947 907 500
PciI A'CATG_T
Cuts at: 3810 3810
Size: 5354
PfoI T'CCnGG_A
Cuts at: 320 320
Size: 5354
PmeI GTTT'AAAC
Cuts at: 2353 2353
Size: 5354
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4493 4525 4493
Size: 32 5322
PsiI TTA'TAA
Cuts at: 392 392
Size: 5354
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1622 1654 1622
Size: 32 5322
PstI C_TGCA'G
Cuts at: 387 561 785 1056 1419 1830 2401 3086 387
Size: 174 224 271 363 411 571 685 2655
Fragments arranged by size:
2655 685 571 411 363 271 224 174
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5354
PvuII CAG'CTG
Cuts at: 1317 1537 2211 2779 3139 1317
Size: 220 674 568 360 3532
Fragments arranged by size:
3532 674 568 360 220
RsrII CG'GwC_CG
Cuts at: 3549 3549
Size: 5354
SacI G_AGCT'C
Cuts at: 2114 2368 2114
Size: 254 5100
SalI G'TCGA_C
Cuts at: 2391 2391
Size: 5354
SapI GCTCTTCn'nnn_
Cuts at: 3377 3587 3377
Size: 210 5144
SbfI CC_TGCA'GG
Cuts at: 2401 2401
Size: 5354
ScaI AGT'ACT
Cuts at: 2047 4871 2047
Size: 2824 2530
SfcI C'TryA_G
Cuts at: 33 383 557 781 1052 1415 1826 2062 2397
Size: 350 174 224 271 363 411 236 335
Cuts at: 2397 3082 4075 4266 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 411 363 350 335 271
236 224 191 174
SfoI GGC'GCC
Cuts at: 2786 3034 2786
Size: 248 5106
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
SmaI CCC'GGG
Cuts at: 2376 2376
Size: 5354
SmlI C'TyrA_G
Cuts at: 2509 3424 3916 4178 2509
Size: 915 492 262 3685
Fragments arranged by size:
3685 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5354
SphI G_CATG'C
Cuts at: 2407 2907 2407
Size: 500 4854
StyI C'CwwG_G
Cuts at: 851 1373 1496 2488 2842 851
Size: 522 123 992 354 3363
Fragments arranged by size:
3363 992 522 354 123
SwaI ATTT'AAAT
Cuts at: 2139 2139
Size: 5354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 534 1211 1915 2937 3070 3529 3738 534
Size: 677 704 1022 133 459 209 2150
Fragments arranged by size:
2150 1022 704 677 459 209 133
TatI w'GTAC_w
Cuts at: 1805 1949 2045 4869 1805
Size: 144 96 2824 2290
Fragments arranged by size:
2824 2290 144 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1826 1858 4871 4903 1826
Size: 32 3013 32 2277
Fragments arranged by size:
3013 2277 32 32
Tth111I GACn'n_nGTC
Cuts at: 3151 3151
Size: 5354
XbaI T'CTAG_A
Cuts at: 2385 2385
Size: 5354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2849 2849
Size: 5354
XmaI C'CCGG_G
Cuts at: 2374 2374
Size: 5354
XmnI GAAnn'nnTTC
Cuts at: 1214 1214
Size: 5354
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PciI PfoI PmeI
PpiI PsiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BfrBI BglI BmgBI BseRI BsiWI BssHII BstEII
BstZ17I CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI XhoI ZraI