Restriction map for
pF1KB5754
(Circular) (Six-base) MAPSORT of: pF1KB5754.seq Check: 359 from: 1 to: 5360
pF1KB5754 5360 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 2398 2398
Size: 5360
Acc65I G'GTAC_C
Cuts at: 2376 2376
Size: 5360
AclI AA'CG_TT
Cuts at: 5200 5200
Size: 5360
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1978 2031 3185 3617 4364 4632 1978
Size: 53 1154 432 747 268 2706
Fragments arranged by size:
2706 1154 747 432 268 53
AflIII A'CryG_T
Cuts at: 2712 3816 4793 2712
Size: 1104 977 3279
Fragments arranged by size:
3279 1104 977
AgeI A'CCGG_T
Cuts at: 3748 3748
Size: 5360
AhdI GACnn_n'nnGTC
Cuts at: 970 970
Size: 5360
AleI CACnn'nnGTG
Cuts at: 193 193
Size: 5360
AlwNI CAG_nnn'CTG
Cuts at: 1690 2665 4232 1690
Size: 975 1567 2818
Fragments arranged by size:
2818 1567 975
ApaLI G'TGCA_C
Cuts at: 4130 4130
Size: 5360
ApoI r'AATT_y
Cuts at: 334 755 1150 1219 1696 2364 4713 4969 334
Size: 421 395 69 477 668 2349 256 725
Fragments arranged by size:
2349 725 668 477 421 395 256 69
AseI AT'TA_AT
Cuts at: 20 860 20
Size: 840 4520
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
AvaI C'yCGr_G
Cuts at: 2380 2380
Size: 5360
BamHI G'GATC_C
Cuts at: 345 2385 345
Size: 2040 3320
BanI G'GyrC_C
Cuts at: 2376 2790 3038 3073 2376
Size: 414 248 35 4663
Fragments arranged by size:
4663 414 248 35
BanII G_rGCy'C
Cuts at: 1763 2374 3404 1763
Size: 611 1030 3719
Fragments arranged by size:
3719 1030 611
BbeI G_GCGC'C
Cuts at: 2794 3042 2794
Size: 248 5112
BbsI GAAGACnn'nnnn_
Cuts at: 577 1093 2271 577
Size: 516 1178 3666
Fragments arranged by size:
3666 1178 516
BciVI GTATCCnnnnn_n'
Cuts at: 1790 3258 4019 5360 1790
Size: 1468 761 1341 1790
Fragments arranged by size:
1790 1468 1341 761
BclI T'GATC_A
Cuts at: 561 2880 561
Size: 2319 3041
BglII A'GATC_T
Cuts at: 2875 4912 2875
Size: 2037 3323
BlpI GC'TnA_GC
Cuts at: 2472 2472
Size: 5360
Bme1580I G_kGCm'C
Cuts at: 2985 3078 4134 2985
Size: 93 1056 4211
Fragments arranged by size:
4211 1056 93
BmgBI CAC'GTC
Cuts at: 1561 1561
Size: 5360
BmrI ACTGGGnnnn_n'
Cuts at: 2572 2653 2988 5132 2572
Size: 81 335 2144 2800
Fragments arranged by size:
2800 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3727 3727
Size: 5360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 2048 215
Size: 1833 3527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2536 3415 3907 4205 2536
Size: 879 492 298 3691
Fragments arranged by size:
3691 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 319 1788 5064 319
Size: 1469 3276 615
Fragments arranged by size:
3276 1469 615
BsaAI yAC'GTr
Cuts at: 184 661 2713 184
Size: 477 2052 2831
Fragments arranged by size:
2831 2052 477
BsaBI GATnn'nnATC
Cuts at: 6 350 2874 4911 6
Size: 344 2524 2037 455
Fragments arranged by size:
2524 2037 455 344
BsaHI Gr'CG_yC
Cuts at: 2791 3039 3741 5237 2791
Size: 248 702 1496 2914
Fragments arranged by size:
2914 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2549 3070 3748 4022 4169 4580 2549
Size: 521 678 274 147 411 3329
Fragments arranged by size:
3329 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 136 166 361 391 136
Size: 30 195 30 5105
Fragments arranged by size:
5105 195 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 868 868
Size: 5360
BseYI C'CCAG_C
Cuts at: 1564 1587 2785 3543 4120 4616 1564
Size: 23 1198 758 577 496 2308
Fragments arranged by size:
2308 1198 758 577 496 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 1055 641
Size: 414 4946
BsiEI CG_ry'CG
Cuts at: 104 2902 2948 4156 104
Size: 2798 46 1208 1308
Fragments arranged by size:
2798 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 149 2374 3152 3342 4134 149
Size: 2225 778 190 792 1375
Fragments arranged by size:
2225 1375 792 778 190
BsmI GAATG_Cn'
Cuts at: 651 2173 651
Size: 1522 3838
Bsp1286I G_dGCh'C
Cuts at: 149 1763 2374 2985 3078 3152 3342 3404 4134
Size: 1614 611 611 93 74 190 62 730
Cuts at: 4134 149
Size: 1375
Fragments arranged by size:
1614 1375 730 611 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4580 4580
Size: 5360
BspHI T'CATG_A
Cuts at: 4536 4536
Size: 5360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1781 2410 2624 2926 3307 1781
Size: 629 214 302 381 3834
Fragments arranged by size:
3834 629 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3652 3706 5190 5355 50
Size: 3602 54 1484 165 55
Fragments arranged by size:
3602 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3272 3272
Size: 5360
BsrFI r'CCGG_y
Cuts at: 3358 3539 3748 3358
Size: 181 209 4970
Fragments arranged by size:
4970 209 181
BssSI C'ACGA_G
Cuts at: 3631 3989 3631
Size: 358 5002
BstAPI GCAn_nnn'nTGC
Cuts at: 2737 2737
Size: 5360
BstBI TT'CG_AA
Cuts at: 3721 3721
Size: 5360
BstYI r'GATC_y
Cuts at: 1 120 234 345 1686 2385 2852 2875 3210
Size: 119 114 111 1341 699 467 23 335
Cuts at: 3210 3456 4468 4554 4566 4912 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1341 1012 699 467 449 346 335 246
119 114 111 86 23 12
BtgI C'CryG_G
Cuts at: 710 2240 710
Size: 1530 3830
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3310 3491 3654 3310
Size: 181 163 5016
Fragments arranged by size:
5016 181 163
BtsI GCAGTG_nn'
Cuts at: 1296 2603 1296
Size: 1307 4053
DraI TTT'AAA
Cuts at: 1977 2359 1977
Size: 382 4978
DrdI GACnn_nn'nnGTC
Cuts at: 3066 3924 3066
Size: 858 4502
EaeI y'GGCC_r
Cuts at: 2033 2945 3119 3510 3537 4882 2033
Size: 912 174 391 27 1345 2511
Fragments arranged by size:
2511 1345 912 391 174 27
EagI C'GGCC_G
Cuts at: 2945 2945
Size: 5360
EarI CTCTTCn'nnn_
Cuts at: 274 2084 3383 3593 274
Size: 1810 1299 210 2041
Fragments arranged by size:
2041 1810 1299 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3568 3878 4024 5170 3568
Size: 310 146 1146 3758
Fragments arranged by size:
3758 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2372 2372
Size: 5360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 1978 2031 2048 3185 3617 4364 4632 215
Size: 1763 53 17 1137 432 747 268 943
Fragments arranged by size:
1763 1137 943 747 432 268 53 17
EcoNI CCTnn'n_nnAGG
Cuts at: 1802 1802
Size: 5360
EcoO109I rG'GnC_Cy
Cuts at: 206 2499 206
Size: 2293 3067
EcoRI G'AATT_C
Cuts at: 755 2364 755
Size: 1609 3751
EcoRV GAT'ATC
Cuts at: 987 987
Size: 5360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 225 639 1932 3379 225
Size: 414 1293 1447 2206
Fragments arranged by size:
2206 1447 1293 414
FspI TGC'GCA
Cuts at: 3141 3141
Size: 5360
HaeII r_GCGC'y
Cuts at: 2794 3042 4064 5043 2794
Size: 248 1022 979 3111
Fragments arranged by size:
3111 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 956 988 1411 1443 2116 2148 3202 3232 3234
Size: 32 423 32 673 32 1054 30 2
Cuts at: 3234 3264 956
Size: 30 3052
Fragments arranged by size:
3052 1054 673 423 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 812 2399 812
Size: 1587 3773
KasI G'GCGC_C
Cuts at: 2790 3038 2790
Size: 248 5112
KpnI G_GTAC'C
Cuts at: 2380 2380
Size: 5360
MluI A'CGCG_T
Cuts at: 4793 4793
Size: 5360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1477 4031 4215 1477
Size: 2554 184 2622
Fragments arranged by size:
2622 2554 184
MscI TGG'CCA
Cuts at: 2035 3121 2035
Size: 1086 4274
MslI CAynn'nnrTG
Cuts at: 139 193 493 2224 3349 3476 139
Size: 54 300 1731 1125 127 2023
Fragments arranged by size:
2023 1731 1125 300 127 54
MspA1I CmG'CkG
Cuts at: 1678 2471 2785 3145 4158 4403 1678
Size: 793 314 360 1013 245 2635
Fragments arranged by size:
2635 1013 793 360 314 245
NaeI GCC'GGC
Cuts at: 3541 3541
Size: 5360
NarI GG'CG_CC
Cuts at: 2791 3039 2791
Size: 248 5112
NcoI C'CATG_G
Cuts at: 710 2240 710
Size: 1530 3830
NgoMIV G'CCGG_C
Cuts at: 3539 3539
Size: 5360
NspI r_CATG'y
Cuts at: 222 2413 2913 3820 222
Size: 2191 500 907 1762
Fragments arranged by size:
2191 1762 907 500
PciI A'CATG_T
Cuts at: 3816 3816
Size: 5360
PmeI GTTT'AAAC
Cuts at: 2359 2359
Size: 5360
PmlI CAC'GTG
Cuts at: 661 661
Size: 5360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 4499
Size: 32 5328
PshAI GACnn'nnGTC
Cuts at: 1856 1856
Size: 5360
PstI C_TGCA'G
Cuts at: 2407 3092 2407
Size: 685 4675
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5360
PvuII CAG'CTG
Cuts at: 1678 2785 3145 1678
Size: 1107 360 3893
Fragments arranged by size:
3893 1107 360
RsrII CG'GwC_CG
Cuts at: 3555 3555
Size: 5360
SacI G_AGCT'C
Cuts at: 2374 2374
Size: 5360
SalI G'TCGA_C
Cuts at: 2397 2397
Size: 5360
SapI GCTCTTCn'nnn_
Cuts at: 3383 3593 3383
Size: 210 5150
SbfI CC_TGCA'GG
Cuts at: 2407 2407
Size: 5360
ScaI AGT'ACT
Cuts at: 4877 4877
Size: 5360
SexAI A'CCwGG_T
Cuts at: 799 799
Size: 5360
SfcI C'TryA_G
Cuts at: 33 1679 2403 3088 4081 4272 33
Size: 1646 724 685 993 191 1121
Fragments arranged by size:
1646 1121 993 724 685 191
SfoI GGC'GCC
Cuts at: 2792 3040 2792
Size: 248 5112
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5360
SmaI CCC'GGG
Cuts at: 2382 2382
Size: 5360
SmlI C'TyrA_G
Cuts at: 2515 3430 3922 4184 2515
Size: 915 492 262 3691
Fragments arranged by size:
3691 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5360
SphI G_CATG'C
Cuts at: 2413 2913 2413
Size: 500 4860
SspI AAT'ATT
Cuts at: 1377 1377
Size: 5360
StyI C'CwwG_G
Cuts at: 710 1520 2074 2240 2494 2848 710
Size: 810 554 166 254 354 3222
Fragments arranged by size:
3222 810 554 354 254 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1267 2943 3076 3535 3744 1267
Size: 1676 133 459 209 2883
Fragments arranged by size:
2883 1676 459 209 133
TatI w'GTAC_w
Cuts at: 2054 4875 2054
Size: 2821 2539
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 139 171 4877 4909 139
Size: 32 4706 32 590
Fragments arranged by size:
4706 590 32 32
Tth111I GACn'n_nGTC
Cuts at: 3157 3157
Size: 5360
XbaI T'CTAG_A
Cuts at: 501 2391 501
Size: 1890 3470
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 396 2855 396
Size: 2459 2901
XmaI C'CCGG_G
Cuts at: 2380 2380
Size: 5360
XmnI GAAnn'nnTTC
Cuts at: 497 574 497
Size: 77 5283
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PmlI PpiI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PpuMI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI