Restriction map for
pF1KB5747
(Circular) (Six-base) MAPSORT of: pF1KB5747.seq Check: 174 from: 1 to: 5534
pF1KB5747 5534 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 953 2572 953
Size: 1619 3915
Acc65I G'GTAC_C
Cuts at: 1738 2550 1738
Size: 812 4722
AclI AA'CG_TT
Cuts at: 5374 5374
Size: 5534
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 858 1318 1571 2344 3359 3791 4538 4806
Size: 644 460 253 773 1015 432 747 268
Cuts at: 4806 214
Size: 942
Fragments arranged by size:
1015 942 773 747 644 460 432 268
253
AfeI AGC'GCT
Cuts at: 529 529
Size: 5534
AflIII A'CryG_T
Cuts at: 2886 3990 4967 2886
Size: 1104 977 3453
Fragments arranged by size:
3453 1104 977
AgeI A'CCGG_T
Cuts at: 3922 3922
Size: 5534
AleI CACnn'nnGTG
Cuts at: 788 788
Size: 5534
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1776 1808 1776
Size: 32 5502
AlwNI CAG_nnn'CTG
Cuts at: 2322 2428 2839 4406 2322
Size: 106 411 1567 3450
Fragments arranged by size:
3450 1567 411 106
ApaLI G'TGCA_C
Cuts at: 4304 4304
Size: 5534
ApoI r'AATT_y
Cuts at: 122 187 562 1866 2005 2098 2298 2366 2460
Size: 65 375 1304 139 93 200 68 94
Cuts at: 2460 2538 4887 5143 122
Size: 78 2349 256 513
Fragments arranged by size:
2349 1304 513 375 256 200 139 94
93 78 68 65
AseI AT'TA_AT
Cuts at: 20 20
Size: 5534
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5534
AvaI C'yCGr_G
Cuts at: 2554 2554
Size: 5534
BamHI G'GATC_C
Cuts at: 2559 2559
Size: 5534
BanI G'GyrC_C
Cuts at: 1431 1738 2550 2964 3212 3247 1431
Size: 307 812 414 248 35 3718
Fragments arranged by size:
3718 812 414 307 248 35
BanII G_rGCy'C
Cuts at: 731 1363 2548 3578 731
Size: 632 1185 1030 2687
Fragments arranged by size:
2687 1185 1030 632
BbeI G_GCGC'C
Cuts at: 2968 3216 2968
Size: 248 5286
BbsI GAAGACnn'nnnn_
Cuts at: 2332 2332
Size: 5534
BbvCI CC'TCA_GC
Cuts at: 2037 2037
Size: 5534
BciVI GTATCCnnnnn_n'
Cuts at: 591 3432 4193 5534 591
Size: 2841 761 1341 591
Fragments arranged by size:
2841 1341 761 591
BclI T'GATC_A
Cuts at: 3054 3054
Size: 5534
BfrBI ATG'CAT
Cuts at: 494 779 1578 494
Size: 285 799 4450
Fragments arranged by size:
4450 799 285
BglII A'GATC_T
Cuts at: 3049 5086 3049
Size: 2037 3497
BlpI GC'TnA_GC
Cuts at: 539 2646 539
Size: 2107 3427
Bme1580I G_kGCm'C
Cuts at: 3159 3252 4308 3159
Size: 93 1056 4385
Fragments arranged by size:
4385 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 543 2746 2827 3162 5306 543
Size: 2203 81 335 2144 771
Fragments arranged by size:
2203 2144 771 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3901 3901
Size: 5534
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 1270 2021 2430 468
Size: 802 751 409 3572
Fragments arranged by size:
3572 802 751 409
Bpu10I CC'TnA_GC
Cuts at: 2037 2037
Size: 5534
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 2710 3589 4081 4379 745
Size: 1965 879 492 298 1900
Fragments arranged by size:
1965 1900 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5238 5238
Size: 5534
BsaAI yAC'GTr
Cuts at: 1636 2887 1636
Size: 1251 4283
BsaBI GATnn'nnATC
Cuts at: 6 3048 5085 6
Size: 3042 2037 455
Fragments arranged by size:
3042 2037 455
BsaHI Gr'CG_yC
Cuts at: 2965 3213 3915 5411 2965
Size: 248 702 1496 3088
Fragments arranged by size:
3088 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2723 3244 3922 4196 4343 4754 2723
Size: 521 678 274 147 411 3503
Fragments arranged by size:
3503 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2180 2210 2343 2373 2180
Size: 30 133 30 5341
Fragments arranged by size:
5341 133 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 187 2183 187
Size: 1996 3538
BseYI C'CCAG_C
Cuts at: 2959 3717 4294 4790 2959
Size: 758 577 496 3703
Fragments arranged by size:
3703 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3076 3122 4330 104
Size: 2972 46 1208 1308
Fragments arranged by size:
2972 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 731 1674 2548 3326 3516 4308 731
Size: 943 874 778 190 792 1957
Fragments arranged by size:
1957 943 874 792 778 190
BsmI GAATG_Cn'
Cuts at: 779 779
Size: 5534
Bsp1286I G_dGCh'C
Cuts at: 731 1363 1674 2548 3159 3252 3326 3516 3578
Size: 632 311 874 611 93 74 190 62
Cuts at: 3578 4308 731
Size: 730 1957
Fragments arranged by size:
1957 874 730 632 611 311 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4754 4754
Size: 5534
BspHI T'CATG_A
Cuts at: 4710 4710
Size: 5534
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2584 2798 3100 3481 2584
Size: 214 302 381 4637
Fragments arranged by size:
4637 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3826 3880 5364 5529 50
Size: 3776 54 1484 165 55
Fragments arranged by size:
3776 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3446 3446
Size: 5534
BsrFI r'CCGG_y
Cuts at: 3532 3713 3922 3532
Size: 181 209 5144
Fragments arranged by size:
5144 209 181
BssSI C'ACGA_G
Cuts at: 3805 4163 3805
Size: 358 5176
BstAPI GCAn_nnn'nTGC
Cuts at: 785 2911 785
Size: 2126 3408
BstBI TT'CG_AA
Cuts at: 3895 3895
Size: 5534
BstXI CCAn_nnnn'nTGG
Cuts at: 1494 2519 1494
Size: 1025 4509
BstYI r'GATC_y
Cuts at: 1 2559 3026 3049 3384 3630 4642 4728 4740
Size: 2558 467 23 335 246 1012 86 12
Cuts at: 4740 5086 1
Size: 346 449
Fragments arranged by size:
2558 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 2291 107
Size: 2184 3350
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3484 3665 3828 3484
Size: 181 163 5190
Fragments arranged by size:
5190 181 163
BtsI GCAGTG_nn'
Cuts at: 783 795 1606 2777 783
Size: 12 811 1171 3540
Fragments arranged by size:
3540 1171 811 12
DraI TTT'AAA
Cuts at: 2533 2533
Size: 5534
DrdI GACnn_nn'nnGTC
Cuts at: 3240 4098 3240
Size: 858 4676
EaeI y'GGCC_r
Cuts at: 685 3119 3293 3684 3711 5056 685
Size: 2434 174 391 27 1345 1163
Fragments arranged by size:
2434 1345 1163 391 174 27
EagI C'GGCC_G
Cuts at: 3119 3119
Size: 5534
EarI CTCTTCn'nnn_
Cuts at: 1196 1337 1834 3557 3767 1196
Size: 141 497 1723 210 2963
Fragments arranged by size:
2963 1723 497 210 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3742 4052 4198 5344 3742
Size: 310 146 1146 3932
Fragments arranged by size:
3932 1146 310 146
EcoICRI GAG'CTC
Cuts at: 729 2546 729
Size: 1817 3717
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 468 858 1270 1318 1571 2021 2344 2430
Size: 254 390 412 48 253 450 323 86
Cuts at: 2430 3359 3791 4538 4806 214
Size: 929 432 747 268 942
Fragments arranged by size:
942 929 747 450 432 412 390 323
268 254 253 86 48
EcoNI CCTnn'n_nnAGG
Cuts at: 142 142
Size: 5534
EcoO109I rG'GnC_Cy
Cuts at: 418 2673 418
Size: 2255 3279
EcoRI G'AATT_C
Cuts at: 1866 2005 2538 1866
Size: 139 533 4862
Fragments arranged by size:
4862 533 139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1253 1527 3553 1253
Size: 274 2026 3234
Fragments arranged by size:
3234 2026 274
FspI TGC'GCA
Cuts at: 3315 3315
Size: 5534
HaeII r_GCGC'y
Cuts at: 531 2968 3216 4238 5217 531
Size: 2437 248 1022 979 848
Fragments arranged by size:
2437 1022 979 848 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1345 1377 1548 1580 1843 1875 3376 3406 3408
Size: 32 171 32 263 32 1501 30 2
Cuts at: 3408 3438 1345
Size: 30 3441
Fragments arranged by size:
3441 1501 263 171 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2573 2573
Size: 5534
KasI G'GCGC_C
Cuts at: 2964 3212 2964
Size: 248 5286
KpnI G_GTAC'C
Cuts at: 1742 2554 1742
Size: 812 4722
MluI A'CGCG_T
Cuts at: 4967 4967
Size: 5534
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1897 4205 4389 1897
Size: 2308 184 3042
Fragments arranged by size:
3042 2308 184
MscI TGG'CCA
Cuts at: 687 3295 687
Size: 2608 2926
MslI CAynn'nnrTG
Cuts at: 484 788 1288 2070 3523 3650 484
Size: 304 500 782 1453 127 2368
Fragments arranged by size:
2368 1453 782 500 304 127
MspA1I CmG'CkG
Cuts at: 558 1909 2041 2645 2959 3319 4332 4577 558
Size: 1351 132 604 314 360 1013 245 1515
Fragments arranged by size:
1515 1351 1013 604 360 314 245 132
NaeI GCC'GGC
Cuts at: 3715 3715
Size: 5534
NarI GG'CG_CC
Cuts at: 2965 3213 2965
Size: 248 5286
NcoI C'CATG_G
Cuts at: 107 2291 107
Size: 2184 3350
NdeI CA'TA_TG
Cuts at: 775 1614 775
Size: 839 4695
NgoMIV G'CCGG_C
Cuts at: 3713 3713
Size: 5534
NsiI A_TGCA'T
Cuts at: 496 781 1580 496
Size: 285 799 4450
Fragments arranged by size:
4450 799 285
NspI r_CATG'y
Cuts at: 494 1582 2587 3087 3994 494
Size: 1088 1005 500 907 2034
Fragments arranged by size:
2034 1088 1005 907 500
PasI CC'CwG_GG
Cuts at: 414 414
Size: 5534
PciI A'CATG_T
Cuts at: 3990 3990
Size: 5534
PflMI CCAn_nnn'nTGG
Cuts at: 1295 1321 1295
Size: 26 5508
PfoI T'CCnGG_A
Cuts at: 137 437 893 2331 137
Size: 300 456 1438 3340
Fragments arranged by size:
3340 1438 456 300
PmeI GTTT'AAAC
Cuts at: 2533 2533
Size: 5534
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4673 4705 4673
Size: 32 5502
PpuMI rG'GwC_Cy
Cuts at: 418 418
Size: 5534
PstI C_TGCA'G
Cuts at: 131 790 2424 2581 3266 131
Size: 659 1634 157 685 2399
Fragments arranged by size:
2399 1634 685 659 157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5534
PvuII CAG'CTG
Cuts at: 558 1909 2041 2959 3319 558
Size: 1351 132 918 360 2773
Fragments arranged by size:
2773 1351 918 360 132
RsrII CG'GwC_CG
Cuts at: 3729 3729
Size: 5534
SacI G_AGCT'C
Cuts at: 731 2548 731
Size: 1817 3717
SalI G'TCGA_C
Cuts at: 2571 2571
Size: 5534
SapI GCTCTTCn'nnn_
Cuts at: 1196 3557 3767 1196
Size: 2361 210 2963
Fragments arranged by size:
2963 2361 210
SbfI CC_TGCA'GG
Cuts at: 2581 2581
Size: 5534
ScaI AGT'ACT
Cuts at: 5051 5051
Size: 5534
SfcI C'TryA_G
Cuts at: 33 127 710 786 2258 2420 2577 3262 4255
Size: 94 583 76 1472 162 157 685 993
Cuts at: 4255 4446 33
Size: 191 1121
Fragments arranged by size:
1472 1121 993 685 583 191 162 157
94 76
SfoI GGC'GCC
Cuts at: 2966 3214 2966
Size: 248 5286
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5534
SmaI CCC'GGG
Cuts at: 2556 2556
Size: 5534
SmlI C'TyrA_G
Cuts at: 724 2689 3604 4096 4358 724
Size: 1965 915 492 262 1900
Fragments arranged by size:
1965 1900 915 492 262
SnaBI TAC'GTA
Cuts at: 1636 1636
Size: 5534
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5534
SphI G_CATG'C
Cuts at: 494 2587 3087 494
Size: 2093 500 2941
Fragments arranged by size:
2941 2093 500
SspI AAT'ATT
Cuts at: 1881 2526 1881
Size: 645 4889
StuI AGG'CCT
Cuts at: 915 915
Size: 5534
StyI C'CwwG_G
Cuts at: 107 395 1487 1927 2291 2500 2668 3022 107
Size: 288 1092 440 364 209 168 354 2619
Fragments arranged by size:
2619 1092 440 364 354 288 209 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 427 3117 3250 3709 3918 427
Size: 2690 133 459 209 2043
Fragments arranged by size:
2690 2043 459 209 133
TatI w'GTAC_w
Cuts at: 218 1911 5049 218
Size: 1693 3138 703
Fragments arranged by size:
3138 1693 703
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 402 434 2180 2212 5051 5083 402
Size: 32 1746 32 2839 32 853
Fragments arranged by size:
2839 1746 853 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3331 3331
Size: 5534
XbaI T'CTAG_A
Cuts at: 2211 2565 2211
Size: 354 5180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2516 3029 2516
Size: 513 5021
XmaI C'CCGG_G
Cuts at: 2554 2554
Size: 5534
XmnI GAAnn'nnTTC
Cuts at: 2429 2429
Size: 5534
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI ApaI AscI AvrII
BaeI BcgI BglI BmgBI BmtI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI PacI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SwaI XhoI
ZraI